EP1979493A1 - Procedes de mesure de l'activite d'une kinase - Google Patents

Procedes de mesure de l'activite d'une kinase

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Publication number
EP1979493A1
EP1979493A1 EP07717310A EP07717310A EP1979493A1 EP 1979493 A1 EP1979493 A1 EP 1979493A1 EP 07717310 A EP07717310 A EP 07717310A EP 07717310 A EP07717310 A EP 07717310A EP 1979493 A1 EP1979493 A1 EP 1979493A1
Authority
EP
European Patent Office
Prior art keywords
substituted
kinase
alkyl
amino
heteroaryl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP07717310A
Other languages
German (de)
English (en)
Other versions
EP1979493A4 (fr
Inventor
Erik M. Schaefer
Xiao-Dong Qian
Min Li
Kyle R. Gee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
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Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Publication of EP1979493A1 publication Critical patent/EP1979493A1/fr
Publication of EP1979493A4 publication Critical patent/EP1979493A4/fr
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Definitions

  • the present invention relates to methods for detecting and/or measuring the activity of a specific kinase of interest.
  • Protein kinases are believed to play a role in the formation of many different diseases. Therefore, new drug candidates and treatment protocols are being identified using methods for understanding and identifying protein kinase activity and molecules that affect that activity.
  • a protein kinase catalyzes the transfer of a phosphate group from adenosine triphosphate (ATP) to a serine, threonine or tyrosine residue in a peptide or protein sequence.
  • ATP adenosine triphosphate
  • Protein kinases are involved in all aspects of regulation within cells. The traditional method for assaying kinase activity is discontinuous and requires 32 P-labelled ATP, which requires special handling.
  • kinase recognition motif or amino acid sequence that a particular kinase recognizes and phosphorylates. Once captured, ATP can be added to the assay and a signaling moiety can then generate or alter a signal upon a phosphorylation event.
  • each kinase may be specific for a particular kinase recognition motif; however, there are some sequence similarities in the amino acid sequences of the kinase recognition motif surrounding the target amino acid among kinases.
  • kinase recognition motif to capture a particular kinase may result in capturing a population of different kinases that all recognize the same or similar kinase recognition motif.
  • the results of these assays have a propensity to generate false positive results when trying to identify and/or measure the activity of a specific kinase.
  • What is needed, therefore, is a method of improving the specificity of the kinase activity assays to lower the incidences of false positives, thus adding value to assay results that assess kinase activity.
  • the present invention relates to methods for detecting and/or measuring the activity of a specific kinase, with the methods comprising contacting one or more kinases with a binding agent to isolate a specific kinase of interest.
  • the isolated kinase is then contacted with a kinase activity sensor, where the kinase activity sensor is comprised of a kinase recognition motif that is capable of being recognized by the isolated kinase, and at least one phosphorylation site.
  • the isolated kinase if present, phosphorylates the amino acid target of the kinase activity sensor and levels of the phosphorylated amino acid target can then be quantified.
  • One aspect of the present invention provides a method of measuring the activity of a specific kinase, said method comprising:
  • kinase activity sensor comprises at least one peptide capable of being phosphorylated and a chelator
  • kinase activity sensor forms a ternary complex with the metal ion and phosphorylated peptide to generate a detectable signal
  • the binding agent comprises an antibody or a functional fragment thereof.
  • the support is glass, plastic, metal, polymeric particle, polymeric gel or a polymeric membrane.
  • the kinase activity sensor has an increased fluorescence signal when complexed with the metal ion.
  • the kinase activity sensor further comprises a signaling moiety that is separate from the chelator. More particular still, the signaling moiety comprises a coumarin, cyanine, benzofuran, a quinoline, a quinazolinone, an indole, a benzazole, a borapolyazaindacene or a xanthene.
  • the chelator comprises a signaling moiety. More particular still, the signaling moiety comprises a coumarin, cyanine, benzofuran, a quinoline, a quinazolinone, an indole, a benzazole, a borapolyazaindacene or a xanthene. In another embodiment, the chelator comprises a quinoline or a derivative thereof, phenanthrolines or derivatives thereof, BAPTA, IDA, DTPA and derivatives thereof.
  • the metal ion is Ca 2+ , Zn 2+ , Mg 2+ , Ga 3+ , Tb 3+ , La 3+ , Pb 2+ , Hg 2+ , Cd 2+ , Cu 2+ , Ni 2+ , Co 2+ , Fe 2+ , Mn 2+ , Ba 2+ , or Sr 2+ .
  • the peptide comprises an amino acid that when phosphorylated complexes magnesium.
  • the method further comprises contacting the immobilized specific kinase with ATP.
  • the kinase activity sensor comprises the formula:
  • R group is -SO 2 X, where X is —OR” or — NR"R"'; R' is hydroxy, amino, or thiol; R" is C 1-6 alkyl; R'" is hydrogen or alkyl; and n is 1, 2 or 3.
  • the kinase activity sensor has the formula:
  • R 1 is H, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, acylamino, acyloxy, amino, substituted amino, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, aminocarbonyloxy, aminosulfonyl, aminosulfonyloxy, aminosulfonylamino, amidino, carboxyl, carboxyl ester, (carboxyl ester)amino, (carboxyl ester)oxy, cyano, halo, hydroxy, nitro, SO 3 H, sulfonyl, substituted sulfonyl, sulfonyloxy, thioacyl, thiol, alkylthio, substituted alkylthio, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, heterocycl
  • R 2 is a fluorophore, H, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, acylamino, acyloxy, amino, substituted amino, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, aminocarbonyloxy, aminosulfonyl, aminosulfonyloxy, aminosulfonylamino, amidino, carboxyl, carboxyl ester, (carboxyl ester)amino, (carboxyl ester)oxy, cyano, halo, hydroxy, nitro, SO 3 H, sulfonyl, substituted sulfonyl, sulfonyloxy, thioacyl, thiol, alkylthio, substituted alkylthio, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloal
  • R 3 is a fluorophore, H, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, acylamino, acyloxy, amino, substituted amino, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, aminocarbonyloxy, aminosulfonyl, aminosulfonyloxy, aminosulfonylamino, amidino, carboxyl, carboxyl ester, (carboxyl ester)amino, (carboxyl ester)oxy, cyano, halo, hydroxy, nitro, SO 3 H, sulfonyl, substituted sulfonyl, sulfonyloxy, thioacyl, thiol, alkylthio, substituted alkylthio, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloal
  • R 4 is a fluorophore, H, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, acylamino, acyloxy, amino, substituted amino, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, aminocarbonyloxy, aminosulfonyl, aminosulfonyloxy, aminosulfonylamino, amidino, carboxyl, carboxyl ester, (carboxyl ester)amino, (carboxyl ester)oxy, cyano, halo, hydroxy, nitro, SO 3 H, sulfonyl, substituted sulfonyl, sulfonyloxy, thioacyl, thiol, alkylthio, substituted alkylthio, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloal
  • R 6 is a fluorophore, H 5 alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, acylamino, acyloxy, amino, substituted amino, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, aminocarbonyloxy, aminosulfonyl, aminosulfonyloxy, aminosulfonylamino, amidino, carboxyl, carboxyl ester, (carboxyl ester)amino, (carboxyl ester)oxy, cyano, halo, hydroxy, nitro, SO 3 H, sulfonyl, substituted sulfonyl, sulfonyloxy, thioacyl, thiol, alkylthio, substituted alkylthio, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloal
  • R 7 is H, alkyl, substituted alkyl, carbonyl, or a protecting group
  • R 8 is H, alkyl, substituted alkyl, carbonyl, or a protecting group
  • L is a linker
  • Y is a peptide; or a tautomer, stereoisomer, or salt thereof.
  • At least one of R 2 , R 3 , R 4 , R 5 and R 6 is a fluorophore.
  • the peptide is at least one of SEQ ID NO. 1 through SEQ ID NO. 133.
  • composition comprising at least one of SEQ ID NO. 1 through SEQ ID NO. 133.
  • composition comprising:
  • a kinase activity sensor comprising at least one peptide capable of being phosphorylated and a chelator.
  • kits for detecting the activity of a specific kinase comprising a binding agent that binds to the specific kinase and a kinase activity sensor, wherein said kinase activity sensor comprises at least one peptide capable of being phosphorylated and a chelator. More particularly, the kit further comprises ATP and a metal ion that has affinity for both the chelator and the phosphorylated peptide.
  • Figure 1 depicts the kinase activity of p38 kinase as assessed using the methods of the present invention.
  • Figure 2 depicts the kinase activity of Erkl/2 kinase as assessed using the methods of the present invention.
  • Figure 3 depicts AMI Activity in Crude Lysates from PDGF-Treated or Control MH3T3 Cells.
  • Figure 4 depicts Akt3 Activity in Crude Lysates from PDGF-Treated or Control NIH3T3 Cells.
  • Figure 5 depicts ERK1/2 Activity in Crude Lysates from PDGF-Treated or Control NIH3T3 Cells
  • Figure 6 depicts p70-S6K Activity in Crude Lysates from PDGF-Treated or Control MCF-7 Cells
  • Figure 7 depicts RSK Activity in Crude Lysates from PMA-Treated or Control HeLa Cells
  • Figure 8 depicts c-Src Activity in Crude Lysates from c-Src-Transfected CEF Cells.
  • Figure 9 depicts B-Raf Activity in Crude Lysates from NGF Treated or Control PC 12
  • Figure 10 depicts ERK1/2 Activity in Crude Lysates from PDGF-Treated or Control NIH3T3 Cells.
  • Figure 11 depicts MEKl Activity in Crude Lysates from PDGF-Treated or Control NIH3T3 Cells.
  • Figure 12 depicts p38 MAPK Activity in Crude Lysates from Anisomycin Treated or Control RAW Cells.
  • Figure 13 depicts Omnia Tyrosine Kinase Recombinant Assays Signal to Noise Ratios for various kinase targets, wherein the kinase recognition motif for each of the figures are as follows: Figure 13A corresponds to SEQ ID NO. 42 (-2.8 fold); Figure 13B corresponds to SEQ ID NO. 43 (-2.2 fold); Figure 13C corresponds to SEQ ID NO. 45 (-2.4 fold); Figure 13D corresponds to SEQ ID NO. 44 (-1.8 fold); Figure 13E corresponds to SEQ ID NO. 46 (-8.3 fold); Figure 13F corresponds to SEQ ID NO. 48 (-6.8 fold); Figure 13G corresponds to SEQ ID NO. 47 (-7.9 fold); and Figure 13H corresponds to SEQ ID NO. 49 (-6.9 fold).
  • FIG. 14 depicts a Cell-based (Magnetic Bead Capture) Kinase Assay for Akt/PKB kinase.
  • Dynabeads M-280 w/Sheep anti-Mouse IgG were used along with Akt Mouse Monoclonal Antibody (Invitrogen). 2ug anti-Akt mAb to 400ug lysate were added and incubated at 4 0 C overnight. 20ul Dynabeads were added to each sample at 4 0 C for 2 hrs and then beads were washed 3X. 2OuM Aktide were added with ImM ATP and results were read in M5 for 5hrs. The isolation and kinase activity sensor increased selectivity and adjustable signal.
  • Alkyl refers to monovalent saturated aliphatic hydrocarbyl groups having from 1 to 10 carbon atoms and preferably 1 to 6 carbon atoms. This term includes, by way of example, linear and branched hydrocarbyl groups such as methyl (CH 3 -), ethyl (CH 3 CH 2 -), n-propyl (CH 3 CH 2 CH 2 -), isopropyl ((CHs) 2 CH-), "-butyl (CH 3 CH 2 CH 2 CH 2 -), isobutyl ((CHs) 2 CHCH 2 -), .sec-butyl ((CH 3 )(CH 3 CH 2 )CH-), t-butyl ((CHs) 3 C-), n-pentyl (CH 3 CH 2 CH 2 CH 2 CH 2 -), and neopentyl ((CH 3 ) 3 CCH 2 -).
  • Substituted alkyl refers to an alkyl group having from 1 to 5, preferably 1 to 3, or more preferably 1 to 2 substituents selected from the group consisting of alkoxy, substituted alkoxy, acyl, acylamino, acyloxy, amino, substituted amino, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, aminocarbonyloxy, aminosulfonyl, aminosulfonyloxy, aminosulfonylamino, amidino, aryl, substituted aryl, aryloxy, substituted aryloxy, arylthio, substituted arylthio, carboxyl, carboxyl ester, (carboxyl ester)amino, (carboxyl ester)oxy, cyano, cycloalkyl, substituted cycloalkyl, cycloalkyloxy, substituted cycloalkyloxy, cycloalkyloxy,
  • Alkoxy refers to the group -O-alkyl wherein alkyl is defined herein. Alkoxy includes, by way of example, methoxy, ethoxy, «-propoxy, isopropoxy, «-butoxy, t-butoxy, sec-bx ⁇ oxy, and «-pentoxy.
  • Substituted alkoxy refers to the group -O-(substituted alkyl) wherein substituted alkyl is defined herein.
  • Acyl refers to the groups H-C(O)-, alkyl-C(O)-, substituted alkyl-C(O)-, alkenyl-C(O)-, substituted alkenyl-C(O)-, alkynyl-C(O)-, substituted alkynyl-C(O)-, cycloalkyl-C(O)-, substituted cycloalkyl-C(O)-, cycloalkenyl-C(O)-, substituted cycloalkenyl-C(O)-, aryl-C(O)-, substituted aryl-C(O)-, heteroaryl-C(O)-, substituted heteroaryl-C(O)-, heterocyclic-C(O)-, and substituted heterocyclic-C(O)-, wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, substituted
  • Acylamino refers to the groups -NRC(O)alkyl, -NRC(O)substituted alkyl, -NRC(O)cycloalkyl, -NRC(O)substituted cycloalkyl, -NRC(O)cycloalkenyl, -NRC(O)substituted cycloalkenyl, -NRC(O)alkenyl, -NRC(O)substituted alkenyl, -NRC(O)alkynyl, - NRC(O)substituted alkynyl, -NRC(O)aryl, -NRC(O)substituted aryl, -NRC(O)heteroaryl, -NRC(O)substituted heteroaryl, -NRC(O)heterocyclic, and -NRC(O)substituted heterocyclic where
  • Acyloxy refers to the groups alkyl-C(O)O-, substituted alkyl-C(O)O-, alkenyl-C(O)O-, substituted alkenyl-C(O)O-, alkynyl-C(O)O-, substituted alkynyl-C(O)O-, aryl-C(O)O-, substituted aryl-C(O)O-, cycloalkyl-C(0)0-, substituted cycloalkyl-C(O)O-, cycloalkenyl-C(O)O-, substituted cycloalkenyl-C(0)0-, heteroaryl-C(O)O-, substituted heteroaryl-C(O)O-, heterocyclic-C(O)O-, and substituted heterocyclic-C(O)O- wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl,
  • Amino refers to the group -NH 2 .
  • Substituted amino refers to the group -NR 5 R" where R' and R" are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, heteroaryl, substituted heteroaryl, heterocyclic, substituted heterocyclic, -SO 2 -alkyl, -SO 2 -substituted alkyl, -SO 2 -alkenyl, -SO 2 -substituted alkenyl, -SO 2 -cycloalkyl, -SCVsubstituted cylcoalkyl, -SO 2 -cycloalkenyl, -SO 2 -substituted cylcoalkenyl, -SO 2
  • R' is hydrogen and R" is alkyl
  • the substituted amino group is sometimes referred to herein as alkylamino.
  • R' and R" are alkyl
  • the substituted amino group is sometimes referred to herein as dialkylamino.
  • a monosubstituted amino it is meant that either R' or R" is hydrogen but not both.
  • a disubstituted amino it is meant that neither R' nor R" are hydrogen.
  • Aminocarbonyl refers to the group -C(O)N R 10 R 11 where R 10 and R 11 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic and where R 10 and R 11 are optionally joined together with the nitrogen bound thereto to form a heterocyclic or substituted heterocyclic group, and wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl,
  • Aminothiocarbonyl refers to the group -C(S)NR 10 R 11 where R 10 and R 11 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic and where R 10 and R 11 are optionally joined together with the nitrogen bound thereto to form a heterocyclic or substituted heterocyclic group, and wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl
  • Aminocarbonylamino refers to the group -NRC(O)NR 10 R 11 where R is hydrogen or alkyl and R 10 and R 11 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic and where R 10 and R 11 are optionally joined together with the nitrogen bound thereto to form a heterocyclic or substituted heterocyclic group, and wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycl
  • Aminothiocarbonylamino refers to the group -NRC(S)NR 10 R 11 where R is hydrogen or alkyl and R 10 and R 11 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic and where R 10 and R 11 are optionally joined together with the nitrogen bound thereto to form a heterocyclic or substituted heterocyclic group, and wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted
  • Aminocarbonyloxy refers to the group -0-C(O)NR 10 R 11 where R 10 and R 11 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic and where R 10 and R 11 are optionally joined together with the nitrogen bound thereto to form a heterocyclic or substituted heterocyclic group, and wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl
  • Aminosulfonyl refers to the group -SO 2 NR 10 R 11 where R 10 and R 11 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic and where R 10 and R 11 are optionally joined together with the nitrogen bound thereto to form a heterocyclic or substituted heterocyclic group, and wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, aryl
  • Aminosulfonyloxy refers to the group -0-SO 2 NR 10 R 11 where R 10 and R 11 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic and where R 10 and R 11 are optionally joined together with the nitrogen bound thereto to form a heterocyclic or substituted heterocyclic group, and wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkenyl, substituted cycloal
  • Aminosulfonylamino refers to the group -NR-SO 2 NR 10 R 11 where R is hydrogen or alkyl and R 10 and R 11 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, cycloalkenyl, substituted cycloalkyenyl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic and where R 10 and R 11 are optionally joined together with the nitrogen bound thereto to form a heterocyclic or substituted heterocyclic group, and wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, cycloalkenyl,
  • Aryl refers to a monovalent aromatic carbocyclic group of from 6 to 14 carbon atoms having a single ring (e.g., phenyl) or multiple condensed rings (e.g., naphthyl or anthryl) which condensed rings may or may not be aromatic (e.g., 2-benzoxazolinone, 2H- 1,4- benzoxazin-3(4H)-one-7-yl, and the like) provided that the point of attachment is at an aromatic carbon atom.
  • Preferred aryl groups include phenyl and naphthyl.
  • Substituted aryl refers to aryl groups which are substituted with 1 to 5, preferably 1 to 3, or more preferably 1 to 2 substituents selected from the group consisting of alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, alkoxy, substituted alkoxy, acyl, acylamino, acyloxy, amino, substituted amino, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, arninothiocarbonylarnino, aminocarbonyloxy, aminosulfonyl, aminosulfonyloxy, aminosulfonylamino, amidino, aryl, substituted aryl, aryloxy, substituted aryloxy, arylthio, substituted arylthio, carboxyl, carboxyl ester, (carboxyl ester)amino, (carboxyl ester)oxy,
  • Aryloxy refers to the group -O-aryl, where aryl is as defined herein, that includes, by way of example, phenoxy and naphthoxy.
  • Substituted aryloxy refers to the group -O-(substituted aryl) where substituted aryl is as defined herein.
  • Arylthio refers to the group -S-aryl, where aryl is as defined herein.
  • Substituted arylthio refers to the group -S-(substituted aryl), where substituted aryl is as defined herein.
  • Alkenyl refers to alkenyl groups having from 2 to 6 carbon atoms and preferably 2 to 4 carbon atoms and having at least 1 and preferably from 1 to 2 sites of alkenyl unsaturation. Such groups are exemplified, for example, by vinyl, allyl, and but-3-en-l-yl.
  • Substituted alkenyl refers to alkenyl groups having from 1 to 3 substituents, and preferably 1 to 2 substituents, selected from the group consisting of alkoxy, substituted alkoxy, acyl, acylamino, acyloxy, amino, substituted amino, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, aminocarbonyloxy, aminosulfonyl, aminosulfonyloxy, aminosulfonylamino, amidino, aryl, substituted aryl, aryloxy, substituted aryloxy, arylthio, substituted arylthio, carboxyl, carboxyl ester, (carboxyl ester)amino, (carboxyl ester)oxy, cyano, cycloalkyl, substituted cycloalkyl, cycloalkyloxy, substituted cycloalkyloxy, cycloalkyloxy,
  • Alkynyl refers to alkynyl groups having from 2 to 6 carbon atoms and preferably 2 to 3 carbon atoms and having at least 1 and preferably from 1 to 2 sites of alkynyl unsaturation.
  • Substituted alkynyl refers to alkynyl groups having from 1 to 3 substituents, and preferably 1 to 2 substituents, selected from the group consisting of alkoxy, substituted alkoxy, acyl, acylamino, acyloxy, amino, substituted amino, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, aminocarbonyloxy, aminosulfonyl, aminosulfonyloxy, aminosulfonylamino, amidino, aryl, substituted aryl, aryloxy, substituted aryloxy, arylthio, substituted arylthio, carboxyl, carboxyl ester, (carboxyl ester)amino, (carboxyl ester)oxy, cyano, cycloalkyl, substituted cycloalkyl, cycloalkyloxy, substituted cycloalkyloxy, cycloalkyloxy
  • Carboxyl or “carboxy” refers to -COOH or salts thereof.
  • Carboxyl ester or “carboxy ester” refers to the groups -C(O)O-alkyl, -C(O)O-substituted alkyl, -C(O)O-alkenyl, -C(O)O-substituted alkenyl, -C(O)O-alkynyl, -C(O)O-substituted alkynyl, -C(O)O-aryl, -C(O)O-substituted aryl, -C(O)O-cycloalkyl, -C(O)O-substituted cycloalkyl, -C(O)O-cycloalkenyl, -C(O)O-substituted cycloalkenyl, -C(O)O-hetero
  • (Carboxyl ester)amino refers to the group -NR-C(O)O-alkyl, substituted -NR-C(O)O-alkyl, -NR-C(O)O-alkenyl, -NR-C(O)O-substituted alkenyl, -NR-C(O)O-alkynyl, -NR-C(O)O-substituted alkynyl, -NR-C(O)O-aryl, -NR-C(O)O-substituted aryl, -NR-C(O)O-cycloalkyl, -NR-C(O)O-substituted cycloalkyl, -NR-C(O)O-cycloalkenyl, - NR-C(O)O-substituted cycloalkenyl, -NR-C(O)O-heteroaryl, -NR-NR-
  • (Carboxyl este ⁇ xy) refers to the group -O-C(O)O-alkyl, substituted -O-C(O)O-alkyl, -O-C(O)O-alkenyl, -O-C(O)O-substituted alkenyl, -O-C(O)O-alkynyl, -O-C(O)O-substituted alkynyl, -O-C(O)O-aryl, -O-C(O)O-substituted aryl, -O-C(O)O-cycloalkyl, -O-C(O)O-substituted cycloalkyl, -O-C(O)O-cycloalkenyl, -O-C(O)O-substituted cycloalkenyl, -O-C(O)O-heteroaryl, -O-C(O)
  • Cyano refers to the group -CN.
  • Cycloalkyl refers to cyclic alkyl groups of from 3 to 10 carbon atoms having single or multiple cyclic rings including fused, bridged, and spiro ring systems. Examples of suitable cycloalkyl groups include, for instance, adamantyl, cyclopropyl, cyclobutyl, cyclopentyl, and cyclooctyl.
  • Substituted cycloalkyl and “substituted cycloalkenyl” refers to a cycloalkyl or cycloalkenyl group having from 1 to 5 or preferably 1 to 3 substituents selected from the group consisting of oxo, thione, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, alkoxy, substituted alkoxy, acyl, acylamino, acyloxy, amino, substituted amino, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, aminocarbonyloxy, aminosulfonyl, aminosulfonyloxy, aminosulfonylamino, amidino, aryl, substituted aryl, aryloxy, substituted aryloxy, arylthio, substituted arylthio, carboxyl, carboxyl, carboxy
  • Cycloalkyloxy refers to -O-cycloalkyl.
  • Substituted cycloalkyloxy refers to -O-(substit ⁇ ted cycloalkyl).
  • Cycloalkylthio refers to -S-cycloalkyl.
  • Substituted cycloalkylthio refers to -S-(substituted cycloalkyl).
  • Cycloalkenyloxy refers to -O-cycloalkenyl.
  • Substituted cycloalkenyloxy refers to -O-(substituted cycloalkenyl).
  • Cycloalkenylthio refers to -S-cycloalkenyl.
  • Substituted cycloalkenylthio refers to -S-(substituted cycloalkenyl).
  • FMOC refers to a compound having the general formula (or derivatives thereof):
  • Halo or "halogen” refers to fluoro, chloro, bromo and iodo.
  • Heteroaryl refers to an aromatic group of from 1 to 10 carbon atoms and 1 to 4 heteroatoms selected from the group consisting of oxygen, nitrogen and sulfur within the ring.
  • Such heteroaryl groups can have a single ring (e.g., pyridinyl or furyl) or multiple condensed rings (e.g., indolizinyl or benzothienyl) wherein the condensed rings may or may not be aromatic and/or contain a heteroatom provided that the point of attachment is through an atom of the aromatic heteroaryl group.
  • the nitrogen and/or the sulfur ring atom(s) of the heteroaryl group are optionally oxidized to provide for the N-oxide (N- ⁇ O), sulf ⁇ nyl, or sulfonyl moieties.
  • Preferred heteroaryls include pyridinyl, pyrrolyl, indolyl, thiophenyl, and furanyl.
  • Substituted heteroaryl refers to heteroaryl groups that are substituted with from 1 to 5, preferably 1 to 3, or more preferably 1 to 2 substituents selected from the group consisting of the same group of substituents defined for substituted aryl.
  • Heteroaryloxy refers to -O-heteroaryl.
  • Substituted heteroaryloxy refers to the group -O-(substituted heteroaryl).
  • Heteroarylthio refers to the group -S-heteroaryl.
  • Substituted heteroarylthio refers to the group -S-(substituted heteroaryl).
  • Heterocycle or “heterocyclic” or “heterocycloalkyl” or “heterocyclyl” refers to a saturated or unsaturated group having a single ring or multiple condensed rings, including fused bridged and spiro ring systems, from 1 to 10 carbon atoms and from 1 to 4 hetero atoms selected from the group consisting of nitrogen, sulfur or oxygen within the ring wherein, in fused ring systems, one or more the rings can be cycloalkyl, aryl or heteroaryl provided that the point of attachment is through the non-aromatic ring.
  • the nitrogen and/or sulfur atom(s) of the heterocyclic group are optionally oxidized to provide for the N-oxide, sulfmyl, sulfonyl moieties.
  • Substituted heterocyclic or “substituted heterocycloalkyl” or “substituted heterocyclyl” refers to heterocyclyl groups that are substituted with from 1 to 5 or preferably 1 to 3 of the same substituents as defined for substituted cycloalkyl.
  • Heterocyclyloxy refers to the group -O-heterocycyl.
  • Substituted heterocyclyloxy refers to the group -O-(substituted heterocycyl).
  • Heterocyclylthio refers to the group -S-heterocycyl.
  • Substituted heterocyclylthio refers to the group -S-(substituted heterocycyl).
  • heterocycle and heteroaryls include, but are not limited to, azetidine, pyrrole, imidazole, pyrazole, pyridine, pyrazine, pyrimidine, pyridazine, indolizine, isoindole, indole, dihydroindole, indazole, purine, quinolizine, isoquinoline, quinoline, phthalazine, naphthylpyridine, quinoxaline, quinazoline, cinnoline, pteridine, carbazole, carboline, phenanthridine, acridine, phenanthroline, isothiazole, phenazine, isoxazole, phenoxazine, phenothiazine, imidazolidine,
  • Niro refers to the group -NO 2 .
  • Spirocyclyl refers to divalent saturated cyclic group from 3 to 10 carbon atoms having a cycloalkyl or heterocyclyl ring with a spiro union (the union formed by a single atom which is the only common member of the rings) as exemplified by the following structure:
  • Sulfonyl refers to the divalent group -S(O) 2 -.
  • Substituted sulfonyl refers to the group -SO 2 -alkyl, -SO 2 -substituted alkyl,
  • Substituted sulfonyl includes groups such as methyl-SO 2 -, phenyl-SO 2 -, and 4-methylphenyl-SO 2 -.
  • “Sulfonyloxy” refers to the group -OSO 2 -alkyl, -OSO 2 -substituted alkyl, -OSO 2 - alkenyl, -OSO 2 -substituted alkenyl, -OSO 2 -cycloalkyl, -OSO 2 -substiruted cylcoalkyl, -OSO 2 - cycloalkenyl, -OSO 2 -substituted cylcoalkenyl,-OSO 2 -aryl, -OSO 2 -substituted aryl, -OSO 2 - heteroaryl, -OS ⁇ 2-substituted heteroaryl, -OS0 2 -heterocyclic,
  • Thioacyl refers to the groups H-C(S)-, alkyl-C(S)-, substituted alkyl-C(S)-, alkenyl-C(S)-, substituted alkenyl-C(S)-, alkynyl-C(S)-, substituted alkynyl-C(S)-, cycloalkyl-C(S)-, substituted cycloalkyl-C(S)-, cycloalkenyl-C(S)-, substituted cycloalkenyl-C(S)-, aryl-C(S)-, substituted aryl-C(S)-, heteroaryl-C(S)-, substituted heteroaryl-C(S)-, heterocyclic-C(S)-, and substituted heterocyclic-C(S)-, wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, substituted
  • Thiol refers to the group -SH.
  • Alkylthio refers to the group -S-alkyl wherein alkyl is as defined herein.
  • Substituted alkylthio refers to the group -S -(substituted alkyl) wherein substituted alkyl is as defined herein.
  • protected or a "protecting group” with respect to hydroxyl groups, amine groups, and sulfhydryl groups refers to forms of these functionalities which are protected from undesirable reaction with a protecting group known to those skilled in the art such as those set forth in Protective Groups in Organic Synthesis, Greene, T. W. , Solon Wiley & Sons, New York, NY, (1st Edition, 1981) which can be added or removed using the procedures set forth therein.
  • Examples of protected hydroxyl groups include, but are not limited to, silyl ethers such as those obtained by reaction of a hydroxyl group with a reagent such as, but not limited to, t- butyldimethyl-chlorosilane, trimethylchlorosilane, triisopropylchlorosilane, triethylchlorosilane; substituted methyl and ethyl ethers such as, but not limited to methoxymethyl ether, methythiomethyl ether, benzyloxymethyl ether, t- butoxymethyl ether, 2- methoxyethoxymethyl ether, tetrahydropyranyl ethers, 1-ethoxyethyl ether, allyl ether, benzyl ether; esters such as, but not limited to, benzoylformate, formate, acetate, trichloroacetate, and trifluoracetate.
  • a reagent such as, but not limited to
  • protected amine groups include, but are not limited to, benzyl or dibenzyl, amides such as, fonnamide, acetamide, trifluoroacetamide, and benzamide; imides, such as phthalimide, BOC (tBoc), FMOC, and dithiosuccinimide; and others.
  • a protecting group for amines is an FMOC group.
  • protected sulfhydryl groups include, but are not limited to, thioethers such as S-benzyl thioether, and S-4-picolyl thioether; substituted S-methyl derivatives such as hemithio, dithio and aminothio acetals; and others.
  • Stereoisomer or “stereoisomers” refer to compounds that differ in the chirality of one or more stereocenters. Stereoisomers include enantiomers and diastereomers.
  • affinity refers to the strength of the binding interaction of two molecules, such as a metal-chelating compound and a metal ion.
  • detectable response refers to an occurrence of, or a change in, a signal that is directly or indirectly detectable either by observation or by instrumentation.
  • the detectable response is an optical response resulting in a change in the wavelength distribution patterns or intensity of absorbance or fluorescence or a change in light scatter, fluorescence lifetime, fluorescence polarization, or a combination of these parameters.
  • the detectable response is an occurrence of a signal wherein the dye is inherently fluorescent and does not produce a change in signal upon binding to a metal ion or phosphorylated target molecule.
  • the detectable response is the result of a signal, such as color, fluorescence, radioactivity or another physical property of the detectable label becoming spatially localized in a subset of a sample such as in a gel, on a blot, or an array, in a well of a micoplate, in a microfluidic chamber, or on a microparticle as the result of formation of a ternary complex of the invention that comprises a phosphorylated target molecule.
  • a signal such as color, fluorescence, radioactivity or another physical property of the detectable label becoming spatially localized in a subset of a sample such as in a gel, on a blot, or an array, in a well of a micoplate, in a microfluidic chamber, or on a microparticle as the result of formation of a ternary complex of the invention that comprises a phosphorylated target molecule.
  • enzyme refers to a protein molecule produced by living organisms, or through chemical modification of a natural protein molecule, that catalyzes chemical reaction of other substances without itself being destroyed or altered upon completion of the reactions.
  • other substances include, but are not limited to chemiluminescent, chromogenic and fluorogenic substances or protein-based substrates.
  • a "kinase” is an enzyme capable of phosphorylating a substrate. Due to the extreme versatility of the methods and components described herein, the present invention finds use in the detection of activity from all kinases.
  • kinase activity sensor refers to a compound or composition comprising at least one peptide substrate capable of being phosphorylated, a chelator and a detection moiety, wherein the detection moiety is the chelator per se or includes an additional detection moiety, such as a fluorophore.
  • a preferred kinase activity sensor of the present invention has the formula:
  • fluorophore refers to a composition that is inherently fluorescent or demonstrates a change in fluorescence upon binding to a biological compound or metal ion, i.e., fluorogenic. Fluorophores may contain substitutents that alter the solubility, spectral properties or physical properties of the fluorophore.
  • fluorophores include, but are not limited to coumarin, cyanine, benzofuran, a quinoline, a quinazolinone, an indole, a benzazole, a borapolyazaindacene and xanthenes including fluoroscein, rhodamine and rhodol as well as semiconductor nanocrystals and other fluorophores described in RICHARD P. HAUGLAND, MOLECULAR PROBES HANDBOOK OF FLUORESCENT PROBES AND RESEARCH CHEMICALS (10 th edition, 2005).
  • linking refers to a divalent moiety capable of linking two particles, either as a single covalent bond or a series of stable covalent bonds incorporating 1-30 nonhydrogen atoms selected from the group consisting of C, N, O, S and P that covalently attach the fluorophore, quinoline group and amino acids to form the present kinase activity sensor.
  • exemplary linking members include a moiety that includes -C(O)NH-, -C(O)O-, -NH-, -S-, -O-, and the like.
  • linkers include covalent bonds, amino acids, succinimidyl derivatives, methines, and alkenyl groups, alkyl and substituted alkyl groups, ethyleyene, proypylene, polyethylene, and polypropylene glycols, esters, ethers, amides, carbamates, and carbonyl containing moieties, diones, squarate, adipic acid as well as other groups, such as those described in Chemistry of Protein Conjugation and Cross-Linking by Susan Wong.
  • a "cleavable linker” is a linker that has one or more cleavable groups that may be broken by the result of a reaction or condition.
  • the term “cleavable group” refers to a moiety that allows for release of a portion, e.g., a reporter molecule, carrier molecule or solid support, of a conjugate from the remainder of the conjugate by cleaving a bond linking the released moiety to the remainder of the conjugate. Such cleavage is either chemical in nature, or enzymatically mediated.
  • Exemplary enzymatically cleavable groups include natural amino acids or peptide sequences that end with a natural amino acid.
  • the present invention relates to methods for detecting and/or measuring the activity of a specific kinase.
  • Specific kinase is used to indicate a known kinase of interest, where at least some information about the activity and/or structure is known to the practitioner prior to implementing the methods of the present invention.
  • the methods comprise contacting a sample comprising one or more kinases with a binding agent to isolate the specific kinase of interest.
  • the one or more kinases may be present in any environment including but not limited to, a cell, a tissue, a body fluid, a buffer solution or another synthetic composition or fluid. Where more than one type of kinase is present, the collection of kinases represents a plurality or population of kinases. Not every member of the population of kinases need be a specific kinase, as understood herein. Rather, the sample, which could comprise a population of different kinases, should comprise or may be suspected to comprise the specific kinase being assayed.
  • binding agent is used to mean a composition that binds specifically to the known biomarker.
  • binding agents include, but are not limited to, receptors, antibodies and functional fragments thereof.
  • antibody is used to mean immunoglobulin molecules and functional fragments thereof, regardless of the source or method of producing the fragment.
  • a "functional fragment" of an immunoglobulin is a portion of the immunoglobulin molecule that specifically binds to a binding target.
  • antibody encompasses whole antibodies, such as antibodies with isotypes that include but are not limited to TgG, TgM, TgA, TgD, TgE and TgY.
  • Whole antibodies may be monoclonal or polyclonal, and they may be humanized or chimeric.
  • the term "monoclonal antibody” as used herein is not limited to antibodies produced through hybridoma technology. Rather the term “monoclonal antibody” refers to an antibody that is derived from a single clone, including any eukaryotic, prokaryotic, or phage clone, and not the method by which it is produced.
  • antibody also encompasses functional fragments of immunoglobulins, including but not limited to Fab fragments, Fab' fragments, F(ab') 2 fragments and Fd fragments. "Antibody” also encompasses fragments of immunoglobulins that comprise at least a portion of a V L and/or V H domain, such as single chain antibodies, a single-chain Fv (scFv), disulfide-linked Fvs and the like.
  • the antibodies used in the present invention may be monospecific, bispecif ⁇ c, trispecific or of even greater multispecificity.
  • the antibodies may be monovalent, bivalent, trivalent or of even greater multivalency.
  • the antibodies of the invention may be from any animal origin including, but not limited to, birds and mammals.
  • the antibodies are human, murine, rat, sheep, rabbit, goat, guinea pig, horse, or chicken.
  • "human” antibodies include antibodies having the amino acid sequence of a human immunoglobulin and include antibodies isolated from human immunoglobulin libraries or from animals transgenic for one or more human immunoglobulin and that do not express endogenous immunoglobulins, as described in United States Patent No. 5,939,598, which is herein incorporated by reference.
  • the antibodies used in the present invention may be described or specified in terms of the epitope(s) or portion(s) of a polypeptide to which they recognize or specifically bind. Or the antibodies may be described based upon their ability to bind to specific conformations of the antigen. In one embodiment, a single antibody used in the methods of the present invention is specific towards an epitope presented on the specific kinase of interest.
  • binding affinities encompassed in the present invention include but are not limited to those with a dissociation constant (Kd) less than 5xlO "2 M, 10 "2 M, 5xlO ⁇ 3 M, 10 "3 M, 5xlO "4 M, 10 "4 M, 5xlO "5 M, 10 "5 M, 5xlO "6 M, 1(T 6 M, 5xlO "7 M, 1(T 7 M, 5xlO "8 M 5 10 "8 M, 5xlO- 9 M, 10 “9 M, 5x10 40 M, 10 "10 M, 5x10 "11 M, 10 "n M, 5x10 -12 M, 1(T 12 M, 5xl O "13 M 5 10 "13 M, 5xlO -14 M 5 10 "14 M 5 5xl(T 15 M
  • the antibodies used in the invention also include derivatives that are modified, for example, by covalent attachment of any type of molecule to the antibody such that covalent attachment does not prevent the antibody from generating an anti-idiotypic response.
  • modifications to antibodies include but are not limited to, glycosylation, acetylation, pegylation, phosphorylation, amidation, derivatization by known protecting/blocking groups, proteolytic cleavage, linkage to a cellular ligand or other composition, such as a signaling moiety, a label etc.
  • the antibodies may be linked or attached to solid substrates, such as, but not limited to, beads, particles, glass surfaces, plastic surfaces, ceramic surfaces, metal surfaces.
  • the antibodies used in the present invention may be generated by any suitable method known in the art.
  • Polyclonal antibodies can be produced by various procedures well known in the art.
  • a kinase or an epitope on the kinase can be administered to various host animals including, but not limited to, rabbits, mice, rats, to induce the production of sera containing polyclonal antibodies specific for the antigen.
  • adjuvants may be used to increase the immunological response, depending on the host species, and include but are not limited to, Freund's (complete and incomplete), mineral gels such as aluminum hydroxide, surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, keyhole limpet hemocyanins, dinitrophenol, and potentially useful human adjuvants such as BCG (bacille Calmette-Guerin) and corynebacteriwn parvum. Such adjuvants are also well known in the art.
  • portions of the peptide chain making up the kinase may be attached to multiarm peptides known as multiple antigentic peptides (MAPs).
  • MAPs multiple antigentic peptides
  • the MAP can then be administered to an animal to generate an immune response that is highly tailored to the epitope presented on the arm or arms of MAP.
  • Methods of generating antibodies using MAP technology are well-known in the art. Methods of generating antibodies using MAP technology is described in Tarn, JP, Proc. Natl Acad. Sd. USA 85: 5409, (1988) and Olive, C. et al., Mini Rev. Med. Chem. 1 :429, (2001), which are incorporated by reference.
  • Monoclonal antibodies can be prepared using a wide variety of techniques known in the art including the use of hybridoma, recombinant, and phage display technologies, or a combination thereof.
  • monoclonal antibodies can be produced using hybridoma techniques including those known in the art and taught, for example, in Harlow et al, Antibodies: A Laboratory Manual, (Cold Spring Harbor Laboratory Press, 2nd ed. 1988); Hammerling, et al, in: Monoclonal Antibodies and T-CeIl Hybridomas 563-681 (Elsevier, N.Y., 1981) (both of which are incorporated by reference).
  • Methods for producing and screening for specific antibodies using hybridoma technology are routine and well known in the art such as, but not limited to, immunizing a mouse. Once an immune response is detected, the mouse spleen is harvested and splenocytes isolated. The splenocytes are then fused by well known techniques to any suitable myeloma cells, for example cells from cell line SP20 available from the ATCC. Hybridomas are selected and cloned by limited dilution. The hybridoma clones can then be assayed by methods known in the art for cells that secrete antibodies capable of binding a biomarker of the present invention. Ascites fluid, which generally contains high levels of antibodies, can be generated by immunizing mice with positive hybridoma clones.
  • the antibodies used in the present invention can also be generated using various phage display methods known in the art.
  • phage display methods functional antibody domains are displayed on the surface of phage particles which carry the polynucleotide sequences encoding them.
  • phage can be utilized to display antigen binding domains expressed from a repertoire or combinatorial antibody library.
  • Phage expressing an antigen binding domain that binds the antigen of interest can be selected or identified with the antigen of interest, such as using a labeled antigen or antigen bound or captured to a solid surface or bead.
  • the phage used in these methods are typically filamentous phage including, but not limited to, fd and Ml 3 binding domains expressed from phage with Fab, Fv or disulfide stabilized Fv antibody domains recombinantly fused to either the phage gene III or gene VIII protein.
  • phage display methods that can be used to make the antibodies of the present invention include those disclosed in Brinkman et al, J. Immunol. Methods 182:41-50 (1995); Ames etal, J. Immunol Methods 184:177-186 (1995); Kettleborough et al, Eur. J.
  • Antibody fragments which recognize specific epitopes may be generated by known techniques.
  • Fab and F(ab') 2 fragments of the invention may be produced by proteolytic cleavage of immunoglobulin molecules, using enzymes such as papain (to produce Fab fragments) or pepsin (to produce F(ab') 2 fragments).
  • F(ab') 2 fragments contain the variable region, the light chain constant region and the CHl domain of the heavy chain.
  • Fab' and F(ab') 2 fragments are disclosed in PCT publication WO 92/22324; Mullinax et al., BioTechniques 12(6):864-869 (1992); and Sawai et al., AJRI 34:26-34 (1995); and Better et al., Science 240: 1041-1043 (1988), which are herein incorporated by reference.
  • the antibody coding regions from the phage can be isolated and used to generate whole antibodies, including human antibodies, or any other desired antigen binding fragment, and expressed in any desired host, including mammalian cells, insect cells, plant cells, yeast, and bacteria.
  • a chimeric antibody is a molecule in which different portions of the antibody are derived from different animal species, such as antibodies having a variable region derived from a murine monoclonal antibody and a human immunoglobulin constant region.
  • Methods for producing chimeric antibodies are known in the art. See e.g., Morrison, Science 229:1202 (1985); Oi et al, BioTechniques 4:214 (1986); Gillies et al, J. Immunol. Methods 125:191-202(1989); United States Patent Nos. 5,807,715; 4,816,567; and 4,816,397, all of which are herein incorporated by reference.
  • Humanized antibodies are antibody molecules from non-human species antibody that binds the desired antigen having one or more complementarity determining regions (CDRs) from the non-human species and framework regions from a human immunoglobulin molecule.
  • CDRs complementarity determining regions
  • framework residues in the human framework regions will be substituted with the corresponding residue from the CDR donor antibody to alter, preferably improve, antigen binding.
  • These framework substitutions are identified by methods well known in the art, e.g., by modeling of the interactions of the CDR and framework residues to identify framework residues important for antigen binding and sequence comparison to identify unusual framework residues at particular positions. (See United States Patent No. 5,585,089; Riechmann et al, Nature 332:323 (1988), both of which are herein incorporated by reference.
  • Antibodies can be humanized using a variety of techniques known in the art including, for example, CDR-grafting (EP 239,400; PCT publication WO 91/09967; United States Patent Nos. 5,225,539; 5,530,101; and 5,585,089), veneering or resurfacing (EP 592,106; EP 519,596; Padlan, Molecular Immunology 28(4/5):489- 498 (1991); Studnicka et al, Protein Engineering 7(6):805-814 (1994); Roguska. etal, Proc. Natl Acad. Set 91:969-913 (1994)), and chain shuffling (United States Patent No. 5,565,332), all of which are hereby incorporated by reference.
  • CDR-grafting EP 239,400; PCT publication WO 91/09967; United States Patent Nos. 5,225,539; 5,530,101; and 5,585,089)
  • veneering or resurfacing EP 592,106; EP 519
  • Human antibodies may be particularly desirable for therapeutic treatment or diagnosis of human patients.
  • Human antibodies can be made by a variety of methods known in the art including phage display methods described above using antibody libraries derived from human immunoglobulin sequences. See also. U.S. Pat. Nos. 4,444,887 and 4,716,111; and PCT publications WO 98/46645, WO 98/50433, WO 98/24893, WO 98/16654, WO 96/34096, WO 96/33735, and WO 91/10741; each of which is incorporated by reference.
  • Human antibodies can also be produced using transgenic mice which are incapable of expressing functional endogenous immunoglobulins, but which can express human immunoglobulin genes.
  • the human heavy and light chain immunoglobulin gene complexes may be introduced randomly or by homologous recombination into mouse embryonic stem cells.
  • the human variable region, constant region, and diversity region may be introduced into mouse embryonic stem cells in addition to the human heavy and light chain genes.
  • the mouse heavy and light chain immunoglobulin genes may be rendered non-functional separately or simultaneously with the introduction of human immunoglobulin loci by homologous recombination. In particular, homozygous deletion of the JH region prevents endogenous antibody production.
  • the modified embryonic stem cells are expanded and microinjected into blastocysts to produce chimeric mice.
  • the chimeric mice are then bred to produce homozygous offspring which express human antibodies.
  • the transgenic mice are immunized in the normal fashion with a selected antigen. Monoclonal antibodies directed against the antigen can be obtained from the immunized, transgenic mice using conventional hybridoma technology.
  • the human immunoglobulin transgenes harbored by the transgenic mice rearrange during B cell differentiation, and subsequently undergo class switching and somatic mutation.
  • Still another approach for generating human antibodies utilizes a technique referred to as guided selection.
  • guided selection a selected non-human monoclonal antibody, e.g., a mouse antibody, is used to guide the selection of a completely human antibody recognizing the same epitope. (Jespers et al., Biotechnology 12:899-903 (1988), herein incorporated by reference).
  • a kinase activity sensor is a composition comprising a kinase recognition motif with at least one phosphorylation site.
  • the phosphorylation site may or may not be present within the kinase recognition motif of the kinase activity sensor.
  • the phosphorylation site of is an amino acids reside that is capable being phosphorylated.
  • the kinase activity sensor comprises a peptide that is capable of being phosphroylated. Examples of amino acids capable of being phosphorylated include, but are not limited to, naturally occurring hydroxyl-containing amino acid residues, such as serine, threonine and tyrosine, and non- naturally occurring hydroxyl-containing amino acid residues.
  • kinase recognition motif can be used in accordance with the present invention. While still functional, kinase recognition motifs with acidic residues may be less desirable because of the acidic conditions that these residues may create during the assay. Examples of recognition motifs that can be used in the kinase activity sensors include but are not limited to, the motifs shown in Table I:
  • PLC Protein Kinase C
  • PLC Protein Kinase C
  • ERKl & ERK2 AHLQRQL(pS)I(dP) AHLQRQLSI(dP) and p38 alpha (vs. beta, gamma, delta) AHLQRQL(pS)I(dP)
  • a preferred embodiment of the present invention provides a composition comprising at least one of SEQ ID NO. 1-133. More particularly, the invention provides a composition comprising or consisting of SEQ ID NO. 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, or SEQ ID NO. 49. More particular still, at least one of SEQ ID NO. 1-133 is used as a kinase recognition motif described herein.
  • the isolated kinase will then phosphorylate the amino acid target of the kinase activity sensor.
  • the inventors have discovered that kinases isolated and immobilized with binding agents are still active enzymes. Thus, the active specific kinase is available to phosphorylate the target amino acid residue or residues.
  • adenosine triphosphate (ATP) and a metal ion that has affinity for the chelator of the kinase activity sensor and the phosphorylated amino acid are added to the assay either, before, during or after, the isolated kinase is contacted with the kinase activity sensor.
  • the metal ion is magnesium, however any metal ion as described above is part of the invention.
  • the kinase activity sensor further comprises a signaling moiety that is capable of generating or altering a signal in response to a phosphorylation event on the target phosphorylation site.
  • the signaling moiety is capable of generating a fluorescent signal. Examples of a signaling moiety include but are not limited to Fluorescent Magnesium Indicators (Molecular Probes, Eugene, Oregon, USA) and metal-binding amino acids that fluoresce upon a chelation event. These amino acids are described in United States Patent No. 6,906,194, which is hereby incorporated by reference.
  • metal chelator is used as it is in the art. Namely, a metal chelator is a compound that can form two or more coordination bonds with metal ion.
  • coordination bond is also well known in the art and is used to indicate a coordinate covalent bond between the metal ion and the chelator.
  • the present metal-chelating moieties are moieties that simultaneously bind metal ions and have affinity for exposed phosphate groups on serine, threonine, or tyrosine residues of the kinase substrate, wherein a ternary complex is formed between the metal-chelating moiety, the metal ion and the phosphorylated serine, threonine, or tyrosine residues.
  • Metal ions that have been found to bind phosphate groups include Ca 2+ , Zn 2+ , Mg 2+ , Ga 3+ , Tb 3+ , La 3+ , Pb 2+ , Hg 2+ , Cd 2+ , Cu 2+ , Ni 2+ , Co 2+ , Fe 2+ , Mn 2+ , Ba 2+ , and Sr 2+ .
  • a preferred metal ion for chelation by the kinase activity sensor of the present invention is magnesium.
  • the metal-chelating moieties must 1) bind metal ions that have affinity for phosphate groups, 2) not interfere with the binding of the metal ion for the phosphate groups and 3) maintain a stable ternary complex.
  • Metal- chelating moieties that fit these three criteria include quinoline or a derivative thereof, phenanthrolines or deriviatives thereof, BAPTA, IDA, DTPA and derivatives thereof.
  • the present kinase activity sensor is represented by the following formula:
  • R 1 , R 2 , R 3 , R 4 , R 5 and R 6 are independently a fluorophore, H, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, acylamino, acyloxy, amino, substituted amino, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, aminocarbonyloxy, aminosulfonyl, aminosulfonyloxy, aminosulfonylamino, amidino, carboxyl, carboxyl ester, (carboxyl ester)amino, (carboxyl ester)oxy, cyano, halo, hydroxy, nitro, SO 3 H, sulfonyl, substituted sulfonyl, sulfonyloxy, thioacyl, thiol, alkylthio, substituted alkylthio, aryl, substituted aryl, heteroary
  • the kinase activity sensor has the formula:
  • R 4 is a sulfonamide group or a fluorophore
  • R 7 and R 8 are independently H, alkyl, substituted alkyl, carbonyl, or FMOC; and L is a linker; and Y is a peptide or H; or a tautomer, stereoisomer, or salt thereof.
  • the kinase activity sensor has the formula:
  • R 7 and R 8 are independently H, alkyl, substituted alkyl, carbonyl, or FMOC; and L is a linker; and Y is a peptide; or a tautomer, stereoisomer, or salt thereof.
  • the fluorophore on the kinase activity sensor has the formula:
  • R 9 and R 10 are each independently a halogen, -SO 3 H, substituted sulfonyl, or H.
  • the kinase activity sensor has the formula
  • R 7 is H, or a protecting group
  • R 9 is a halogen, -SO3H, substituted sulfonyl, or H;
  • R 10 is a halogen, -SO 3 H, substituted sulfonyl, or H;
  • L is a linker
  • Y is a peptide or H
  • R 4 is a fluorophore.
  • the fluorophore is dansyl, xanthene, cyanine, borapolyazaindacene, pyrene, naphthalene, coumarin, oxazine, or derivatives thereof.
  • the fluorophore is a coumarin, a xanthene or a derivative thereof.
  • R 1 is hydroxy.
  • R 2 , R 3 , R 5 and R 6 are all H.
  • R 7 is H or FMOC.
  • R 8 is H.
  • Y is a peptide.
  • the peptide comprises a kinase recognition site and at least one amino acid residue selected from serine, threonine, or tyrosine that is subject to phosphorylation by a kinase.
  • the position indicated with a ⁇ bond is in the S configuration; alternatively it is in the R-configuration; alternatively the kinase activity sensor is a racemate.
  • the salt of the kinase activity sensor is magnesium.
  • L is a covalent bond.
  • Y is H.
  • R 9 and R 10 are both fluorine.
  • the present fluorophores can be any fluorophore known in the art that when conjugated to a chelating moiety are fluorogenic or essentially non-fluorescence.
  • a fluorophore of the present invention is any chemical moiety that exhibits an absorption maximum beyond 280 nm, that when part of a kinase activity sensor compound retains its unique spectral properties to provide a detectable signal.
  • the present fluorphores are a separate moiety from the chelating moiety of the kinase activity sensor.
  • the chelator is also the fluorophore.
  • fluorophores examples include, but are not limited to; a pyrene, an anthracene, a naphthalene, an acridine, a stilbene, an indole or benzindole, an oxazole or benzoxazole, a thiazole or benzothiazole, a 4-amino-7-nitrobenz-2- oxa-1, 3-diazole (NBD), a carbocyanine (including any corresponding compounds in US Serial Nos. 09/557,275; 09/968,401 and 09/969,853 and US patent Nos.
  • oxazines include resorufms (including any corresponding compounds disclosed in 5,242,805), aminooxazinones, diaminooxazines, and their benzo-substituted analogs.
  • the fluorophore of the present invention is selected from the group consisting of acridine, anthracene, benzofuran, indole, dansyl, cyanine, borapolyazaindacene, pyrene, naphthalene, coumarin, oxazine, boron dipyror ⁇ ethene difhioride, and xanthenes, including but not limited to fluorescein, rhodamine, and rhodol, and derivatives thereof Additional fluorophores that may be used in the present invention are listed in Richard P.
  • the xanthenes are fluorescein, or rhodamine.
  • the fluorophores may be substituted to adjust solubility, spectral or other physical properties.
  • the fluorophore may, but need not be, a fluorescein, a rhodol (including any corresponding compounds disclosed in US Patent Nos. 5,227,487 and 5,442,045), a rosamine or a rhodamine (including any corresponding compounds in US Patent Nos. 5,798,276; 5,846,737; 5,847,162; 6,017,712; 6,025,505; 6,080,852; 6,716,979; 6,562,632).
  • fluorescein includes benzo- or dibenzofluoresceins, seminaphthofluoresceins, or naphthofluoresceins.
  • rhodol includes seminaphthorhodafluors (including any corresponding compounds disclosed in U.S. Patent No. 4,945,171).
  • Fluorinated xanthene fluorophores have been described previously as possessing particularly useful fluorescence properties (Int. Publ. No. WO 97/39064 and U.S. Patent No. 6,162,931) including those sold under the tradename OREGON GREEN.
  • the Oregon Green precursor will allow for strategic placement of a very bright and highly absorbing pH-insensitive fluorescein derivative in peptide kinase substrates.
  • the fluorescence will initially be quenched via PET by the 8-hydroxyquinoline moiety, but chelation induced fluorescence increase, mediated by magnesium(II) ion will be observed upon phosphorylation of the appropriate Ser/Thr/Tyr residue that is separated from the dye by a beta-turn dipeptide sequence.
  • the fluorescence (490 nm excitation/520 nm emission) increase will be similar in mechanism and magnitude to that observed upon using Fluo-4 for calcium measurements.
  • the fluorophore-substituted amino acid is placed two amino acid residues away from the serine to be phosphorylated so that the metal chelating portion of the Oregon Green alanine derivative can be oriented toward the phosphoserine moiety.
  • the hydroxyquinoline-fluorophore moiety is constructed so that the fluorescence is quenched in the absence of metal ion chelation, and the hydroxyquinoline-amino acid connection point is also chosen for optimal geometry.
  • the fluorophore will contain one or more aromatic or heteroaromatic rings, that are optionally substituted one or more times by a variety of substituents, including without limitation, halogen, nitro, sulfo, cyano, alkyl, perfluoroalkyl, alkoxy, alkenyl, alkynyl, cycloalkyl, arylalkyl, acyl, aryl or heteroaryl ring system, benzo, or other substituents typically present on chromophores or fluorophores known in the art.
  • substituents including without limitation, halogen, nitro, sulfo, cyano, alkyl, perfluoroalkyl, alkoxy, alkenyl, alkynyl, cycloalkyl, arylalkyl, acyl, aryl or heteroaryl ring system, benzo, or other substituents typically present on chromophores or fluorophores known in the art.
  • the fluorophores are independently substituted by substituents selected from the group consisting of hydrogen, halogen, amino, substituted amino, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkoxy, sulfo, reactive group and carrier molecule.
  • the xanthene fluorophores of this invention comprise both compounds substituted and unsubstituted on the carbon atom of the central ring of the xanthene by substituents typically found in the xanthene-based fluorophores such as phenyl and substituted-phenyl moieties.
  • the fluorophores used in the amino acids and compositions of the present invention are rhodamine, fluorescein, dansyl, naphthalene and derivatives thereof.
  • the choice of the fluorophore attached to the chelating moiety will determine the absorption and fluorescence emission properties of the amino acids and compositions of the present invention as well as its live cell properties.
  • Selected sulfonated fluorophores also exhibit advantageous properties, and include sulfonated pyrenes, coumarins, carbocyanines, and xanthenes (as described in U.S. Patent Nos. 5,132,432; 5,696,157; 5,268,486; 6,130,101). Sulfonated pyrenes and coumarins are typically excited at wavelengths below about 450 nm (US Patent Nos. 5,132,432 and 5,696,157).
  • the label is a fluorophore selected from the group consisting of fluorescein, coumarins, rhodamines, 5-TMRIA (tetramethylrhodamine-5-iodoacetamide), (9- (2(or4)-(N-(2-maleimdylethyl)-sulfonamidyl)-4(or 2)-sulfophenyl)-2,3,6,7,12,13,16,17- octahydro-(lH 5 5H,llH,15H-xan.theno(23,4-ij:5,6,7-i'j')(iiquinolizm-18-ium salt) (Texas Red®), 2-(5-( 1 -(6-(N-(2-maleimdylethyl)-amino)-6-oxohexyl)- 1 ,3-dihydro-3 ,3 -dimethyl-5-sulfo-2H- in
  • the fluorophore has a Stokes shift larger than about
  • the fluorophore has a Stokes shift larger than about 100 nm or larger than about 150 nm. In still more embodiments, the present compounds have a Stokes shift larger than about 200 nm or larger than about 250 nm.
  • the term "Stokes shift” as used herein refers to the difference in wavelength between absorbed and emitted energy. Specifically, the Stokes shift is the difference (usually in frequency units) between the spectral positions and the band maxima (or band origin) of the absorption and luminescence arising from the same electronic transitions.
  • S/T or Y peptide substrate sampler plates with 14 substrates (1 substrate per well and 6 wells per substrate) dried onto the surface of a 96-well plate are provided. Each substrate is resuspended in reaction buffer and the kinase(s) of interest are added to each well, followed by monitoring the increase in fluorescence in real time.
  • Another embodiment of the present invention provides a method of detecting kinase activity comprising
  • a kinase activity sensor comprising a metal chelator, one or more amino acids and a fluorophore, wherein the chelator comprises a quinoline group and both the fluorophore and amino acid are conjugated to the quinoline group and, and wherein the amino acids comprise a kinase recognition site and a phosphorylation site;
  • kinase activity sensor comprises at least one peptide capable of being phosphorylated and a chelator
  • kinase activity sensor forms a ternary complex with the metal ion and phosphorylated peptide to generate a detectable signal
  • the measured fluorescence is selected from the group consisting of intensity, excitation or emission wavelength, distribution of fluorescence, fluorescence lifetime, fluorescence polarization, or a combination thereof.
  • Another embodiment of the present invention provides a method of detecting kinase activity, wherein the method comprises: contacting one or more kinases with a binding agent to isolate said specific kinase, thereby forming an isolated kinase;
  • kinase activity sensor comprises a kinase recognition motif that is capable of being recognized by said isolated kinase, and at least one phosphorylation site;
  • Scheme 1 provides an illustration of one embodiment of the present invention employing a kinase activity sensor:
  • the magnesium complex modifies the emission and/or excitation spectrum of the kinase activity sensor, such that phophorylation of the peptide can be detected and/or quantified.
  • the present kinase activity sensors comprise one or more amino acids.
  • the amino acids form a peptide that comprise at least one kinase recognition motif, wherein the kinase recognition motifs comprise at least one phosphorylatable amino acid residue.
  • Any kinase recognition motif can be used in accordance with the present invention.
  • Recognition sequences with acidic residues may show a lesser magnitude of fluorescence increase upon phosphorylation than comparable sequences, as the affinity of the unphosphorylated peptide for magnesium tends to increase under acidic conditions.
  • Phosphorylation sites within the kinase recognition motif accordance with the present invention include, but are not limited to, amino acids comprising hydroxyl groups. Examples include, but are not limited to, naturally occurring hydroxyl-containing amino acid residues, such as serine, threonine and tyrosine, as well as non-naturally occurring hydroxyl-containing amino acid residues.
  • Preferred recognition motifs are listed in Tables I, II, and III.
  • phosphorylation site specific antibodies that can recognize and bind to phosphorylated amino acid residues.
  • antibodies that recognize phosphorylated amino acid residues include those antibodies described in United Stated Patent Application Publication No. 2003/0162330, Mandel, J., Phosphorylation State-Specific Antibodies, Amer. J. Pathol., 163:1687 (2003) and Glenney, JR etal, Monoclonal antibodies to phosphotyrosin. J. Immunol. Methods. 109:277 (1988), which are hereby incorporated by reference.
  • the methods of the present invention are useful for drug discovery settings.
  • the methods of the present invention comprise exposing a cell or tissue to compound, such as drug or drug candidate, prior to isolating the specific kinase of interest.
  • compound such as drug or drug candidate
  • the collection of kinases is present in the cells or tissue and the drug or drug candidate is administered to the cells or tissue.
  • the cells tissue can be lysed and the specific kinase of interest can then be isolated and its activity quantified using the methods of the present invention.
  • the compounds or binding agents (e.g. antibodies) of the invention are bonded to a solid or semi-solid support.
  • a support suitable for use in the present invention is typically substantially insoluble in liquid phases.
  • Solid or semi-solid supports of the current invention are not limited to a specific type of support. Rather, a large number of supports are available and are known to one of ordinary skill in the art.
  • useful solid supports include semi-solids, such as aerogels and hydrogels, resins, beads, biochips (including thin film coated biochips), multi-well plates (also referred to as microtitre plates), membranes, conducting and nonconducting metals and magnetic supports.
  • useful solid supports include silica gels, polymeric membranes, particles, derivatized plastic films, glass beads, cotton, plastic beads, alumina gels, polysaccharides such as Sepharose, poly(acrylate), polystyrene, poly(acrylamide), polyol, agarose, agar, cellulose, dextran, starch, FICOLL, heparin, glycogen, amylopectin, mannan, inulin, nitrocellulose, diazocellulose, polyvinylchloride, polypropylene, polyethylene (including poly(ethylene glycol)), nylon, latex bead, magnetic bead, paramagnetic bead, superparamagnetic bead (Dynabeads (e.g. M-280)), starch and the like.
  • Preferred supports include agarose and magnetic beads.
  • the solid or semi-solid support may include a support reactive functional group, including, but not limited to, hydroxyl, carboxyl, amino, thiol, aldehyde, halogen, nitro, cyano, amido, urea, carbonate, carbamate, isocyanate, sulfone, sulfonate, sulfonamide, sulfoxide, etc., for attaching the compounds of the invention.
  • the solid supports contain a nucleophile (x), such as amino, thiol, or hydroxyl.
  • a suitable solid or semi-solid support can be selected on the basis of desired end use and suitability for various synthetic protocols.
  • resins generally useful in peptide synthesis may be employed, such as polystyrene (e.g., PAM-resin obtained from Bachem Inc., Peninsula Laboratories, etc.), POLYHIPETM resin (obtained from Aminotech, Canada), polyamide resin (obtained from Peninsula Laboratories), polystyrene resin grafted with polyethylene glycol (TentaGelTM, Rapp Polymere, Tubingen, Germany), polydimethyl- acrylamide resin (available from Milligen/Biosearch, California), or PEGA beads (obtained from Polymer Laboratories).
  • polystyrene e.g., PAM-resin obtained from Bachem Inc., Peninsula Laboratories, etc.
  • POLYHIPETM resin obtained from Aminotech, Canada
  • polyamide resin obtained from Peninsula Laboratories
  • kits comprising the compositions described herein for use in detection of kinase activity.
  • the kits include instructions on how to best detect the kinase of interest.
  • kits for detecting the activity of a specific kinase comprising a binding agent that binds to the specific kinase and a kinase activity sensor, wherein said kinase activity sensor comprises at least one peptide capable of being phosphorylated and a chelator. More particularly, the kit further comprises ATP and a metal ion that has affinity for both the chelator and the phosphorylated peptide.
  • S/T or Y peptide substrate sampler plates with 14 substrates (1 substrate per well and 6 wells per substrate) dried onto the surface of a 96-well plate are provided. Each substrate is resuspended in reaction buffer and the kinase(s) of interest are added to each well, followed by monitoring the increase in fluorescence in real time.
  • a preferred kit of the invention includes 14 Sox-based peptide substrates that can be used to measure the activity of a variety of Ser/Thr kinases.
  • the peptides are arranged in sets of 6 replicates with a bottom row of 12 replicates of a control peptide. These substrates correspond to peptides listed in SEQ ID NOs 20-33.
  • Another preferred kit includes 14 Sox-based peptide substrates (kinase activity sensors) that can be used to measure the activity of a variety of Ser/Thr kinases.
  • the peptides are arranged in sets of 6 replicates with a bottom row of 12 replicates of a control peptide. These substrates correspond to peptides listed in SEQ ID NOs 4 & 34-36.
  • additional kinase activity sensors for detection of Ser/Thr kinases.
  • Another preferred kit includes 14 different kinase activity sensors that can be used to measure the activity of a variety of Tyr kinases.
  • the peptides are arranged in sets of 6 replicates with a bottom row of 12 replicates of a control peptide. These substrates correspond to peptides listed in SEQ ID NOs 42-49.
  • additional kinase activity sensors for the detection of tyrosine kinases.
  • kits and/or stabilizers are present in the kit components. Further components include gels, beads, supports, reagents and the like.
  • the kits comprise indicator solutions or indicator "dipsticks", blotters, culture media, cuvettes, and the like.
  • the kits comprise indicator cartridges (where a kit component is bound to a solid support) for use in an automated detector.
  • the kit further comprises addititives, wherein said additives are selected from phosphorylated and non- phosphorylated polypeptides, calcium-binding and non-calcium binding polypeptides, sulfonated and non- sulfonated polypeptides, and sialylated and non-sialylated polypeptides.
  • the kit further comprises a member selected from a fixing solution, a detection reagent, a standard, a wash solution, and combinations thereof.
  • the sample or medium in which the complex of the phosphorylated amino acid, metal ion and chelator of the kinase activity sensor of the present invention is present is illuminated with a wavelength of light selected to give a detectable optical response, and observed with a means for detecting the optical response.
  • Equipment that is useful for illuminating the present compounds and compositions of the invention includes, but is not limited to, hand-held ultraviolet lamps, mercury arc lamps, xenon lamps, lasers and laser diodes. These illumination sources are optically integrated into laser scanners, fluorescence microplate readers or standard or microfluorometers.
  • the the kinase activity sensor of the invention may, at any time after or during an assay, be illuminated with a wavelength of light that results in a detectable optical response, and observed with a means for detecting the optical response.
  • Selected equipment that is useful for illuminating the kinase activity sensor of the invention includes, but is not limited to, hand-held ultraviolet lamps, mercury arc lamps, xenon lamps, argon lasers, laser diodes, and YAG lasers. These illumination sources are optionally integrated into laser scanners, fluorescence microplate readers, standard or mini fluorometers, or chromatographic detectors.
  • This fluorescence emission is optionally detected by visual inspection, or by use of any of the following devices: CCD cameras, video cameras, photographic film, laser scanning devices, fluorometers, photodiodes, quantum counters, epifluorescence microscopes, scanning microscopes, flow cytometers, fluorescence microplate readers, or by means for amplifying the signal such as photomultiplier tubes.
  • a mouse monoclonal antibody specific for p38 kinase was attached to the wells of a 96-well plate.
  • the concentrations of p38 antibody in carbonate buffer were 3 ⁇ g/ml, 6 ⁇ g/ml and 12 ⁇ g/ml and 100 ⁇ l of each concentration was used per well.
  • Murine macrophage cells (RAW264.7) were exposed to anisomycine and incubated for about 2 hours in culture. Control cells were untreated. After incubation, cell culture was removed from the cells and the cells were washed. Cells were then lysed using commercially available cell lysis reagents, and the cell lysate was added to the wells of the coated plate.
  • the kinase activity sensor was added to the wells (50 ng) along with ImM ATP.
  • the kinase recognition motif of the activity sensor comprised the amino acid sequence : AHLQRLSI(dP) (SEQ ID NO. 134), where the serine was the phosphorylation target site.
  • the kinase activity sensor further comprised the metal binding amino acid that is described in United States Patent No. 6,906,194 and referred to as SOX.
  • SOX amino acid fluoresces upon chelation of magnesium.
  • Figure 1 depicts levels phosphorylation activity that can be solely attributed to p38.
  • a rabbit monoclonal antibody specific for Erkl/2 kinase was attached to the wells of a 96-well plate.
  • the concentrations of Erkl/2 antibody in carbonate buffer were 3 ⁇ g/ml, 6 ⁇ g/ml and 12 ⁇ g/ml and 100 ⁇ l of each concentration was used per well.
  • Murine embryonic fibroblast cells (3T3) were exposed to platelet derived growth factor (PDGF) and incubated for about 2 hours in culture. Control cells were untreated. After incubation, cell culture was removed from the cells and the cells were washed. Cells were then lysed using commercially available cell lysis reagents, and the cell lysate was added to the wells of the coated plate.
  • PDGF platelet derived growth factor
  • the kinase activity sensor was added to the wells (50 ng) along with ImM ATP.
  • the kinase recognition motif of the activity sensor comprised the amino acid sequence : AHLQRLSI(dP) (SEQ ID NO. 134), where the serine was the phosphorylation target site.
  • the kinase activity sensor further comprised the metal binding amino acid that is described in United States Patent No. 6,906,194 and referred to as SOX. The SOX amino acid fluoresces upon chelation of magnesium.
  • Figure 2 depicts levels phosphorylation activity that can be solely attributed to Erkl/2 kinase.
  • Example 3 Measurement ofAktl Activity in Crude Lysates from PDGF-Treated or Control NIH3T3 Cells.
  • NIH3T3 cells were seeded in 100 mm dishes and grown in DMEM plus 10% fetal bovine serum until 90% confluent. The cells were then incubated overnight in serum-free medium to induce quiescence, followed by treatment with PDGF A/B (50 ng/mL, 10 min) or control media. Cell lysates were prepared and Aktl activity was assayed as described below in the assay procedure.
  • the PDGF-treated sample showed a reaction rate of 1.13 RFU/sec, whereas the control treated sample had a markedly reduced rate of 0.12 RFU/sec, resulting in a dramatic signal-to-noise ratio of 9.
  • Other assay control groups (bead only group or bead and antibody only group) also had reaction rates less than 0.12 RFU/sec. Activity was assayed as described in the Assay Procedure section below using SEQ ID NO. 25. Results are shown in Figure 3.
  • Wash Buffer (prepare Ix stock): Dilute an appropriate amount of the 10x Wash
  • Buffer Concentrate 10-fold with ultrapure water (e.g., 5 nxL of 10x Wash Buffer + 45 mL of ultrapure water).
  • OmniaTM Kinase Reaction Buffer (prepare Ix stock): Dilute an appropriate amount of the 10x OmniaTM Kinase Reaction Buffer 10-fold with ultrapure water and add DTT (provided) to a final concentration of 0.2 mM (e.g., 500 ⁇ L of 10x OmniaTM Kinase Reaction Buffer + 10 ⁇ L of 100 mM DTT solution + 4490 ⁇ L ultrapure water).
  • a useful reaction buffer for phosphorylation Ser/Thr kinases comprises: 20 mM Tris, pH 7.5; 15 mM MgCl 2 ; 1 mM EGTA; 5 mM beta-glycerophosphate; 5% glycerol; 1 mM ATP (added from separate stock); 0.2 mM DTT (added from separate stock; 1.5 mM CaCl 2 (PKC only; added from separate stock); 2.5 ug/mL phosphatidylserine (PKC only; added from separate stock); and 0.5 ug/mL diacylglycerol (PKC only; added from separate stock).
  • Tyr kinases comprises: 20 mM Tris, pH 7.5; 5 mM MgCl 2 ; 1 mM EGTA; 5 mM beta- glycerophosphate; 5% glycerol; 1 mM ATP (added from separate stock); and 0.2 mM DTT (added from separate stock).
  • Kinase Activity Sensor (prepare 100 ⁇ M stock): Dilute an appropriate amount of the peptide substrate solution (1 mM) 10-fold with Ix OmniaTM Kinase Reaction Buffer (e.g., 10 ⁇ L of 1 mM peptide + 90 ⁇ L of Ix OmniamKinase Reaction Buffer).
  • ATP Solution (prepare 2 mM stock): Dilute an appropriate amount of 100 mM
  • Ix OmniaTM Kinase Reaction Buffer e.g. 10 ⁇ L of 100 mM ATP + 490 ⁇ L of Ix OmniaTM Kinase Reaction Buffer.
  • Cell lysates Dilute the lysate to 0.5 to 1 mg/mL total protein with OmniaTM Cell
  • Lysis Buffer The amount of cell lysate protein used in the assay varies depending on the quantity and activity of kinase enzyme in the individual cell line.
  • a useful buffer for preserving the kinase activity of kinases from crude cell or tissue lysates comprises: 50 mM Tris, pH 7.5; 150 mM NaCl; 2 mM EGTA; 30 mM NaF; 10 mM Na 4 P 2 O 7 ; 100 mM Na 3 VO 4 ; 1% Triton X-100; 50 mM b-glycerophosphate; 1 mM DTT; Sigma Protease Inhibitor cocktail (Cat. # P8340) and Sigma Phosphatase Inhibitor cocktail 1 (Cat. # P2850).
  • Kinase Mix (prepare Ix stock): Dilute an appropriate amount of the Kinase solution with Ix OmniaTM Kinase Reaction Buffer (e.g., 10 ⁇ L of Kinase Mix + 290 ⁇ L of Ix Kinase Reaction Buffer).
  • Ix OmniaTM Kinase Reaction Buffer e.g. 10 ⁇ L of Kinase Mix + 290 ⁇ L of Ix Kinase Reaction Buffer.
  • Kinase control Recombinant kinase enzyme can be used as a positive control to quantify the activity of kinase in the cell lysates.
  • Cell Lysis Buffer is added to each sample well in the antibody coated plate strips. The plates are sealed with Adhesive Plate Cover Strips and incubated at room temperature for 2 hours. Cell lysate is removed and discarded and wells and plates are washed multiple times with 200 ⁇ L of Wash Buffer. Wash buffer is aspirated or decanted and any residual liquid is removed between washes by tapping the plate firmly on absorbent paper. [00213] 30 ⁇ L of the kinase mix is added to 20 ⁇ L of the kinase activity sensor solution
  • the final concentration of kinase activity sensor in the reaction mixture is 20 ⁇ M and the final concentration of ATP is 1 mM.
  • the final volume of the reaction is 100 ⁇ L.
  • the solution is incubated at 3O 0 C for 1 hour.
  • the plate is transferred to a fluorescence plate reader (such as SpectraMax M5® by Molecular Devices or comparable instrument).
  • the fluorescence values are read from each well every 30 seconds at an excitation wavelength of 360 nm and an emission wavelength of 485 nm for up to 5 hours at 3O 0 C in a kinetic mode.
  • Example 4 Measurement ofAktS Activity in Crude Lysates from PDGF-Treated or Control NIH3T3 Cells.
  • NIH3T3 cells were seeded in 100 mm dishes and grown in DMEM plus 10% fetal bovine serum until 90% confluent. The cells were then incubated overnight in serum-free medium to induce quiescence, followed by treatment with PDGF A/B (50 ng/mL, 10 rnin) or control media. Cell lysates were prepared and Akt3 activity was assayed as described in the Assay Procedure of Example 3. The PDGF-treated sample showed a reaction rate of 1.24 RFU/see, whereas the control treated sample had a markedly reduced rate of 0.28 RFU/sec, resulting in a dramatic signal-to-noise ratio of 4.42.
  • PDGF A/B 50 ng/mL, 10 rnin
  • NIH3T3 cells were seeded in 100 mm dishes and grown in DMEM plus 10% fetal bovine serum until 90% confluent. The cells were then incubated overnight in serum-free medium to induce quiescence, followed by treatment with PDGF A/B (50 ng/mL, 10 min) or control media. Cell lysates were prepared and ERK1/2 activity was assayed as described in the Assay Procedure of Example 3. The PDGF-treated sample showed a reaction rate of 1.55 RFU/sec, whereas the control treated sample had a markedly reduced rate of 0.27 RFU/sec, resulting in a dramatic signal-to-noise ratio of 5.7.
  • MCF-7 cells were seeded in 100 mm dishes and grown in DMEM plus 10% fetal bovine serum until 90% confluent. The cells were then incubated overnight in serum-free medium to induce quiescence, followed by treatment with insulin (100 nM, 10 min) or control media. Cell lysates were prepared and p70-S6K activity was assayed as described in the Assay Procedure of Example 3. The insulin-treated sample showed a reaction rate of 1.13 RFU/sec, whereas the control treated sample had a markedly reduced rate of 0.12 RFU/sec, resulting in a dramatic signal-to-noise ratio of 9.4. Other assay control groups (bead only group or bead and antibody only group) also had reaction rates less than 0.12 RFU/sec. Activity was assayed as described in the Assay Procedure section using SEQ ID NO. 42.
  • Example 7 Measurement of RSK Activity in Crude Lysates from PMA-Treated or
  • HeLa cells were seeded in 100 mm dishes and grown in DMEM plus 10% fetal bovine serum until 90% confluent. The cells were then incubated overnight in serum-free medium to induce quiescence, followed by treatment with PMA(200 nM, 30 min) or control media. Cell lysates were prepared and RSK activity was assayed as described in the Assay Procedure of Example 3. The PMA-treated sample showed a reaction rate of 3.17 RFU/sec, whereas the control treated sample had a markedly reduced rate of 0.64 RFU/sec, resulting in a dramatic signal-to-noise ratio of 4.95. Other assay control groups (bead only group or bead and antibody only group) also had reaction rates less than 0.64 RFU/sec. Activity was assayed as described in the Assay Procedure section using SEQ ID NO. 30. Results are shown in Figure 7.
  • C-Src transfected and mock transfected chicken embryo fibroblast (CEF) cell lysates were prepared as described (Thomas, et al, (1999) J. Biol. Chem., 74:36684-36692).
  • the c-Src transfected CEF sample showed a reaction rate of 50 RFU/sec, whereas the control treated sample had a markedly reduced rate of 0.048 RFU/sec, resulting in a dramatic signal-to-noise ratio of 10.5.
  • Other assay control groups (bead only group or bead and antibody only group) also had reaction rates less than 0.05 RFU/sec.
  • Activity was assayed as described in the Assay Procedure of Example 3 using SEQ ID NO. 48. Results are shown in Figure 8.
  • Example 9 Measurement of B-Raf Activity in Crude Lysatesfrom NGF Treated or Control PC 12 Cells.
  • PC12 cells were seeded in 100 mm Petri dishes and grown in DMEM plus 2.5% fetal bovine serum and 15% horse serum. Cells were then treated with NGF (50 ng/mL, 15 minutes) or with control media. Cell lysates were prepared and B-Raf activity was assayed as described in Example 3. The NGF-treated sample produced fluorescence signals that reached a plateau at 150 minutes of incubation (3200 RFU), while the control group reached a plateau at 240 minutes after the reaction started. Activity was assayed as described in the Assay Procedure section using a cascade reaction with SEQ ID NO. 27, used to measure the level of activated MK2 enzyme. Results are shown in Figure 9.
  • NIH3T3 cells were seeded in 100 mm Petri dishes and grown in DMEM plus 10% fetal bovine serum. Cells then were treated with PDGF (50 ng/mL, 15 minutes) or with control media. Cell lysates then were prepared and ERKl/2 activity was assayed as described in Example 1. The PDGF-treated sample produced a fluorescence signal of 3000 RFU that reached a plateau at 335 minutes of incubation, while the control group produced fluorescence signal of 845 RFU. Activity was assayed as described in the Example 3 using a cascade reaction with SEQ ID NO. 27 used to measure the level of activated MK2 enzyme. Results are shown in Figure 10.
  • NIH3T3 cells were seeded in 100 mm Petri dishes and grown in DMEM plus
  • Example 12 Measurement ofp38 MAPK Activity in Crude Lysates from
  • RAW cells were seeded in 100 mm Petri dishes and grown in DMEM plus 10% fetal bovine serum. Cells then were treated with anisomycin (10 ⁇ g/mL, 15 minutes) or with control media. Cell lysates then were prepared and p38 MAPK activity was assayed as described in the Assay Procedures of Example 3. The anisomycin treated sample produced fluorescence signal of 3350 RFU that reached a plateau at 60 minutes of incubation, while the control group produced fluorescence signal of 1250 RFU. Activity was assayed as described in the Assay Procedure section using a cascade reaction with SEQ ID NO. 27 used to measure the level of activated MK2 enzyme. Results are shown in Figure 12.

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Abstract

La présente invention concerne des procédés de détection et/ou de mesure de l'activité d'une kinase spécifique, ces procédés consistant à mettre en contact une ou plusieurs kinases avec un agent de liaison afin d'isoler une kinase spécifique présentant un intérêt. La kinase isolée est ensuite mise en contact avec un capteur d'activité kinase qui est lui-même constitué d'un motif de reconnaissance pour la kinase pouvant être reconnu par la kinase isolée et d'un site de phosphorylation. La kinase isolée phosphoryle la cible acide aminé du capteur d'activité kinase et les niveaux de l'acide aminé cible phosphorylé peuvent ainsi être quantifiés.
EP07717310A 2006-01-18 2007-01-18 Procedes de mesure de l'activite d'une kinase Withdrawn EP1979493A4 (fr)

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US7964729B2 (en) * 2006-08-28 2011-06-21 Massachusetts Institute Of Technology Sox-based kinase sensor
JP2011505847A (ja) * 2007-12-20 2011-03-03 エフ．ホフマン−ラ ロシュ アーゲー 遺伝毒性の予測方法
WO2010040024A2 (fr) * 2008-10-03 2010-04-08 President And Fellows Of Harvard College Procédés, compositions et kits pour le criblage d’activité kinase à rendement élevé utilisant la spectrométrie de masse et des isotopes stables
WO2011025546A1 (fr) 2009-08-31 2011-03-03 Massachusetts Institute Of Technology 1, 2, 3-triazole contenant des détecteurs de protéine kinase
KR101740026B1 (ko) 2016-07-07 2017-05-26 원광대학교산학협력단 4-(4-메틸피페라지닐)스티릴피리딘 유도체가 다중 결합된 라텍스 비드 복합체 및 이를 유효성분으로 포함하는 표적물질 검출용 키트
KR102127289B1 (ko) * 2018-09-18 2020-06-26 고려대학교 산학협력단 암 세포를 표적화하기 위한 plk 선택적 형광 프로브 화합물 및 이를 포함하는 plk 검출용 형광 센서

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