EP1944080A1 - Appareil et procédé pour mouvement d'un liquide dans une cavité - Google Patents

Appareil et procédé pour mouvement d'un liquide dans une cavité Download PDF

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Publication number
EP1944080A1
EP1944080A1 EP07000479A EP07000479A EP1944080A1 EP 1944080 A1 EP1944080 A1 EP 1944080A1 EP 07000479 A EP07000479 A EP 07000479A EP 07000479 A EP07000479 A EP 07000479A EP 1944080 A1 EP1944080 A1 EP 1944080A1
Authority
EP
European Patent Office
Prior art keywords
container
cavity
movement
flow guide
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP07000479A
Other languages
German (de)
English (en)
Inventor
Stephan Korner
Emad Sarofim
Vuk Siljegovic
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Original Assignee
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG, Roche Diagnostics GmbH filed Critical F Hoffmann La Roche AG
Priority to EP07000479A priority Critical patent/EP1944080A1/fr
Publication of EP1944080A1 publication Critical patent/EP1944080A1/fr
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/40—Parts or components, e.g. receptacles, feeding or discharging means
    • B01F29/401—Receptacles, e.g. provided with liners
    • B01F29/402—Receptacles, e.g. provided with liners characterised by the relative disposition or configuration of the interior of the receptacles
    • B01F29/4022—Configuration of the interior
    • B01F29/40221—Configuration of the interior provided with baffles, plates or bars on the wall or the bottom
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/80—Mixers with rotating receptacles rotating about a substantially vertical axis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/10—Mixers with shaking, oscillating, or vibrating mechanisms with a mixing receptacle rotating alternately in opposite directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/24—Mixing the contents of independent containers, e.g. test tubes the containers being submitted to a rectilinear movement
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/30—Driving arrangements; Transmissions; Couplings; Brakes
    • B01F35/33—Transmissions; Means for modifying the speed or direction of rotation
    • B01F35/331—Transmissions; Means for modifying the speed or direction of rotation alternately changing the speed of rotation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/50—Mixing receptacles
    • B01F35/53—Mixing receptacles characterised by the configuration of the interior, e.g. baffles for facilitating the mixing of components
    • B01F35/531—Mixing receptacles characterised by the configuration of the interior, e.g. baffles for facilitating the mixing of components with baffles, plates or bars on the wall or the bottom
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Definitions

  • the invention relates to a device for moving a liquid in a cavity, in particular for mixing, dissolving, dispersing, emulsifying, suspending or homogenizing a substance, with a cavity optionally confining the opening under the provision of an opening, which at least one projecting from a container wall and in having the cavity projecting static deflecting body, and a container on a trajectory cyclically forward and backward moving actuator.
  • the invention further relates to a corresponding method for moving a liquid.
  • the object of the invention is to avoid the disadvantages of the prior art and to optimize a device or a method of the type specified in the sense of improved mixing efficiency and reduced contamination, with an aim of the invention also being to provide a suitable device Mixing container consists.
  • a manufacturing technology advantageous embodiment provides that the deflecting body is integrally formed on the container wall.
  • the flow guide surfaces are at mutually different angles of attack on the adjacent container wall. It is advantageous if the angles of attack in the range between 0 ° and 85 ° with respect to the solder in the base of the flow on the container wall, and if the difference in the angle of attack of the associated flow control between 5 ° and 85 °.
  • the flow control surfaces are curved or arched.
  • the desired effect is achieved if the mutually associated flow control surfaces with respect to the movement path have a different average curvature or slope.
  • the flow guide surfaces then do not run with respect to a center plane perpendicular to the movement path mirror-symmetrical to each other.
  • a further increase in efficiency can be achieved in that a plurality of deflecting bodies are arranged in the shape of a sawtooth or matrix on the container wall.
  • the volume of the cavity is preferably between 5 ⁇ l and 50 ml.
  • the movement path is at least partially linear or circular.
  • complex container movements can be performed, such as two mutually perpendicular linear movements that are performed simultaneously or sequentially or temporally nested.
  • the amplitude of the linear movement of the container is in a range of 20 microns to 20 mm, or the amplitude of the circular movement of the container in a range of 0.5 ° to nx 360 °, where n is a natural number is.
  • the frequency of the movement can also be in a wide range of 0.1 to 10,000 Hz.
  • the container is designed as a disposable article preferably for receiving body fluids to be examined.
  • the container For parallel processing, it is also possible for the container to have a multiplicity of cavities, in particular in the form of a microtiter plate.
  • the container In order to carry out analytical investigations, it is possible for the container to have a test surface which is designed in particular as a sensor and can be acted upon by the moving liquid.
  • the opening may form an inlet or outlet.
  • the actuator comprises a holder for the container and a coupled thereto, oscillating drive unit.
  • a further improvement can be achieved in that the actuator is formed by a laboratory device comprising a control device.
  • the invention also relates to a container having at least one deflecting body projecting on the inside for use in a device according to the invention.
  • the deflecting body does not have mirror-symmetrical flow guidance surfaces.
  • the object mentioned in the introduction is achieved by introducing the liquid into the cavity defined by a container is, and the container is moved cyclically back and forth on a trajectory, wherein at least one deflecting protrudes into the cavity, and wherein the liquid is deflected during the container movement by at least two relative to the trajectory differently oriented flow control surfaces of the deflecting and / or the container is moved in a forward and backward phase of the cyclic container movement with phase-wise different course.
  • the apparatus shown in the drawing enables an effective flow movement of a liquid, in particular for mixing, dissolving, dispersing, emulsifying, suspending or homogenizing a further substance in the liquid.
  • the device 10 comprises a container 12 with at least one cavity 14 for receiving the liquid 16 and an actuator 18 for a cyclic forward and backward running Mixed movement of the container 12 on an at least partially linear or curved trajectory.
  • the container 12 in the respective cavity 14 projecting deflecting body 20.
  • These are integrally formed on a container wall 22 and have at least two relative to the movement path or movement axis (double arrow 24) differently oriented inclined flow guide 26, 28. It is also conceivable that the flow guide are arranged mirror-symmetrically with respect to a plane of symmetry perpendicular to the movement path, and the mixing movement is different in the forward and reverse phases, as explained below.
  • the actuator 18 comprises a holder 30 for the container 12 and a coupled to the holder, oscillating drive unit 32.
  • this arrangement is formed by a laboratory device, which also has a control device 34 for an automatic process sequence.
  • Fig. 2 shows a section in the movement axis of the linear forward or backward movement (arrows 24 ', 24'') in the region of the deflecting body 20.
  • Their flow guide surfaces 26, 28 run towards the interior of the cavity 14 asymmetrically toward each other in a wedge shape. Accordingly own the angle of attack ⁇ 1,2 can be determined, for example, with respect to the solder 29 at the base of the respective flow guide 26, 28 on the container wall 22.
  • the flow guide surface 28 has an angle of incidence ⁇ 2 of approximately 57 °, while the angle of incidence ⁇ 1 of the steeper flow guide surface 26 is approximately 10 ° with respect to the vertical 29 on the container wall 22.
  • the transmission of kinetic energy to the liquid 16 is primarily in the immediate vicinity of the oscillating flow guide 26, 28. Due to the inertia of the liquid, a shock wave is generated, which propagates in the cavity. As illustrated by arrows 36, 38, the generated pulse has a different direction in the forward phase 24 'and the back phase 24 ". Since in this case the pulse from the forward phase 24 'is not completely compensated by an opposite pulse from the back phase 24 ", the liquid undergoes a net movement In this way, an effective swirling of the liquid can be achieved with static deflection bodies 20, ie fixed on the container wall ,
  • the amplitude of the linear movement phases 24 'and 24''of the container 12 is in a range of 20 microns to 20 mm. Accordingly, the frequency the movement can be selected in a wider range from 0.1 to 10,000 Hz.
  • FIG. 3 to 12 and 14 Further embodiments of containers 12 with asymmetric deflecting bodies 20 are shown by way of example. Identical parts are provided with the same reference numerals, as described above.
  • Fig. 3 shows a cuvette-like configuration of a container 12, in which the deflecting body 20 is formed asymmetrically with respect to the axis of a (horizontal) linear movement.
  • the volume of the cavity 14 bounded by the container can be in a wide range between 5 ⁇ l and 50 ml.
  • Such a container may be formed as a disposable for receiving body fluids to be examined.
  • Fig. 4 shows a cylindrical container 12 for a circular oscillating movement (double arrow 24) about the container axis 40.
  • the movement amplitude can be in a wide range of 0.5 ° to nx 360 °, where n is a natural number.
  • the flow guide 26, 28 in their transverse orientation with respect to the circular path of movement 24 are different steep.
  • Fig. 5a and b show container 12 similar to Fig. 3 but with a plurality of on a container wall 22nd Sawtooth-shaped deflecting bodies 20.
  • the path of movement 24 extends horizontally, wherein the deflection body 20 are arranged on the bottom side
  • Fig. 5b illustrates a vertical trajectory 24 in the gravity axis, wherein the deflecting body 20 protrude from a side wall 22.
  • deflectors 20 may be arranged side by side to provide with counter-oriented flow control surfaces for additional mixing flows in the cavity 14.
  • a plurality of deflecting bodies 20 are arranged like a matrix on the container bottom.
  • the mixing movement of the container 12 takes place in two mutually perpendicular trajectories 24 1, 2 in such a way that the irregularly tetrahedral deflecting bodies 20 each have at least two flow guide surfaces oriented differently relative to the respective trajectory.
  • Fig. 8 shows a similar embodiment with prismatic deflecting bodies 20.
  • the flow guide 26,28 perpendicular to the container bottom, but at different angles with respect to the trajectory 24th
  • mixing vessels 12 are shown, which are provided for a circular mixing movement.
  • the deflection body 20 are here on the bottom side or coat side into the container inside, with different steep flow guide surfaces 26, 28 provide in the circular motion for a resulting liquid flow.
  • the cylindrical container 12 are provided with an upper end opening 42 to fill the mix. In principle, it is also conceivable that the containers 12 have an inlet and outlet for the continuous loading of the cavity.
  • the container 12 has a test area 44, for example in the form of an electrode or reagent layer, which responds to an analyte in the liquid within the cavity 14.
  • the mixing bodies 20 ensure an effective flow over the test surface 24.
  • Fig. 12 shows a further embodiment of a container 12, wherein the cavity 14 annularly rotates about an inner structure 46.
  • Fig. 13 shows a container 12, in which a deflecting body 20 is configured symmetrically with respect to the axis of rotation 48 of the circular trajectory 24.
  • the Flow guide surfaces 26, 28 provide here, due to a phase-wise different course of motion for a resulting fluid flow.
  • the loci (rotation angle plotted against time) of the movement phases in the clockwise and counterclockwise direction are not mirror images or congruent.
  • the movement in one direction of rotation can be faster than in the other direction of rotation.
  • Such an asymmetrical course of motion can also be realized for a linear forward and backward movement.
  • FIGS. 14 and 15 show a disc-shaped container assembly which is rotatable about the central axis 48 back and forth and laterally closed cavities 14, in which deflecting body 20 provide for a mixing movement of the liquid via an inlet channel 50 einleitbaren. The displaced upon introduction of the liquid air can escape via an outlet channel 52.
  • dry chemicals can react with an analyte, so that the arrangement operates as it were a mini-laboratory.
  • the cavities 14 are bounded on all sides in the disk-shaped container 12, keeping the mouths of the channels 50, 52 free.
  • the container 12 is composed of a base part 54 and a lid 56.
  • the base member 54 includes the cavities 14 including the baffles 20 and the channels 50, 52 as laterally applied (punched-in) recesses, while the lid 56 provides a laterally sealed termination in the joint plane.
  • the base member 54 and the lid 56 are connected to each other by suitable bonding methods such as gluing, laser welding, thermal sealing, ultrasonic welding together. Such an arrangement can be provided as a disposable part or disposable.
  • the base part 54 may suitably consist of an injection-molded part made of a thermoplastic material, preferably polystyrene (PS), polycarbonate (PC), polymethymethacrylate (PMMA), polypropylene (PP), polyethylene (PE) or cyclo-olefin copolymer (COC).
  • a thermoplastic material preferably polystyrene (PS), polycarbonate (PC), polymethymethacrylate (PMMA), polypropylene (PP), polyethylene (PE) or cyclo-olefin copolymer (COC).
  • PS polystyrene
  • PC polycarbonate
  • PMMA polymethymethacrylate
  • PP polypropylene
  • PE polyethylene
  • COC cyclo-olefin copolymer

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP07000479A 2007-01-11 2007-01-11 Appareil et procédé pour mouvement d'un liquide dans une cavité Ceased EP1944080A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07000479A EP1944080A1 (fr) 2007-01-11 2007-01-11 Appareil et procédé pour mouvement d'un liquide dans une cavité

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP07000479A EP1944080A1 (fr) 2007-01-11 2007-01-11 Appareil et procédé pour mouvement d'un liquide dans une cavité

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EP1944080A1 true EP1944080A1 (fr) 2008-07-16

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008008256A1 (de) * 2007-10-08 2009-04-09 M2P-Labs Gmbh Mikroreaktor
WO2011026559A1 (fr) * 2009-09-05 2011-03-10 Lonza Biologics Plc Système de plaque de puits profonds à couvercle
FR3002462A1 (fr) * 2013-02-25 2014-08-29 Jean-Pierre Solignac Procede et dispositif de melange avec au moins une cuve tournante integrant au moins un element mecanique fixe
CN105195054A (zh) * 2015-09-28 2015-12-30 苏州中亚油墨有限公司 一种油墨振动搅拌混合机
WO2016042204A1 (fr) * 2014-09-16 2016-03-24 Outotec (Finland) Oy Appareil de mélange et son utilisation
EP3030701A1 (fr) * 2013-08-08 2016-06-15 Ricoh Company, Ltd. Appareil et procédé pour la fabrication de cristal de nitrure d'élément du groupe 13
WO2016208575A1 (fr) * 2015-06-25 2016-12-29 シャープ株式会社 Cuve d'agitation et dispositif d'agitation
US20230120357A1 (en) * 2020-05-22 2023-04-20 Politecnico Di Milano Device, method and composition for transfection of cells with nucleic acids

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US5225164A (en) * 1991-09-30 1993-07-06 Astle Thomas W Microplate laboratory tray with rectilinear wells
DE29911370U1 (de) * 1999-07-01 1999-10-07 Sueverkruep Richard Schüttelapparatur zur Prüfung der Redispergierbarkeit von Suspensionen, Dispersionen bzw. Verflüssigung von thixotropen Gelen
WO2000072902A1 (fr) * 1999-05-28 2000-12-07 Integrated Biosystems Decongelation amelioree de solutions biopharmaceutiques sous l'effet d'un mouvement oscillatoire
WO2001083093A1 (fr) * 2000-05-03 2001-11-08 Silva Joe D Procede et dispositif pour produire des formulations posologiques liquides
WO2002087761A1 (fr) * 2001-04-26 2002-11-07 Vrije Universiteit Brussel Procede d'acceleration et intensification de la liaison du recepteur a la cible et dispositifs correspondants
DE10158645A1 (de) * 2001-11-22 2003-06-12 Jerini Ag Wannenförmiges Inkubationsgefäß
US20060013063A1 (en) * 2004-07-19 2006-01-19 Vijay Singh Method and apparatus for resonant wave mixing in closed containers
US20060187746A1 (en) * 2005-01-27 2006-08-24 Graham Packaging Company, L.P. Agitator base for plastic container

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5225164A (en) * 1991-09-30 1993-07-06 Astle Thomas W Microplate laboratory tray with rectilinear wells
WO2000072902A1 (fr) * 1999-05-28 2000-12-07 Integrated Biosystems Decongelation amelioree de solutions biopharmaceutiques sous l'effet d'un mouvement oscillatoire
DE29911370U1 (de) * 1999-07-01 1999-10-07 Sueverkruep Richard Schüttelapparatur zur Prüfung der Redispergierbarkeit von Suspensionen, Dispersionen bzw. Verflüssigung von thixotropen Gelen
WO2001083093A1 (fr) * 2000-05-03 2001-11-08 Silva Joe D Procede et dispositif pour produire des formulations posologiques liquides
WO2002087761A1 (fr) * 2001-04-26 2002-11-07 Vrije Universiteit Brussel Procede d'acceleration et intensification de la liaison du recepteur a la cible et dispositifs correspondants
DE10158645A1 (de) * 2001-11-22 2003-06-12 Jerini Ag Wannenförmiges Inkubationsgefäß
US20060013063A1 (en) * 2004-07-19 2006-01-19 Vijay Singh Method and apparatus for resonant wave mixing in closed containers
US20060187746A1 (en) * 2005-01-27 2006-08-24 Graham Packaging Company, L.P. Agitator base for plastic container

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8828337B2 (en) 2007-10-08 2014-09-09 M2P-Labs Gmbh Microreactor
WO2009046697A2 (fr) * 2007-10-08 2009-04-16 M2P-Labs Gmbh Microréacteur
WO2009046697A3 (fr) * 2007-10-08 2009-09-11 M2P-Labs Gmbh Microréacteur
JP2011501689A (ja) * 2007-10-08 2011-01-13 エム２ピー−ラブス ゲーエムベーハー マイクロリアクタ
DE102008008256A1 (de) * 2007-10-08 2009-04-09 M2P-Labs Gmbh Mikroreaktor
US9783770B2 (en) 2009-09-05 2017-10-10 Lonza Biologics Plc Deepwell plate system with lid
JP2013504041A (ja) * 2009-09-05 2013-02-04 ロンザ バイオロジクス ピーエルシー 蓋付きディープウェルプレートシステム
CN102481572A (zh) * 2009-09-05 2012-05-30 英国龙沙生物医药股份有限公司 有罩的深孔板系统
WO2011026559A1 (fr) * 2009-09-05 2011-03-10 Lonza Biologics Plc Système de plaque de puits profonds à couvercle
FR3002462A1 (fr) * 2013-02-25 2014-08-29 Jean-Pierre Solignac Procede et dispositif de melange avec au moins une cuve tournante integrant au moins un element mecanique fixe
CN105745365A (zh) * 2013-08-08 2016-07-06 株式会社理光 用于制造第13族氮化物晶体的设备和方法
EP3030701A1 (fr) * 2013-08-08 2016-06-15 Ricoh Company, Ltd. Appareil et procédé pour la fabrication de cristal de nitrure d'élément du groupe 13
EP3030701A4 (fr) * 2013-08-08 2016-09-21 Ricoh Co Ltd Appareil et procédé pour la fabrication de cristal de nitrure d'élément du groupe 13
WO2016042204A1 (fr) * 2014-09-16 2016-03-24 Outotec (Finland) Oy Appareil de mélange et son utilisation
EP3406330A1 (fr) * 2014-09-16 2018-11-28 Outotec (Finland) Oy Appareil de mélange et son utilisation
EA032019B1 (ru) * 2014-09-16 2019-03-29 Оутотек (Финлэнд) Ой Смеситель и его применение
US10744471B2 (en) 2014-09-16 2020-08-18 Outotec (Finland) Oy Mixing apparatus and its use
WO2016208575A1 (fr) * 2015-06-25 2016-12-29 シャープ株式会社 Cuve d'agitation et dispositif d'agitation
CN105195054A (zh) * 2015-09-28 2015-12-30 苏州中亚油墨有限公司 一种油墨振动搅拌混合机
US20230120357A1 (en) * 2020-05-22 2023-04-20 Politecnico Di Milano Device, method and composition for transfection of cells with nucleic acids

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