EP1831670A4 - Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences - Google Patents

Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences

Info

Publication number
EP1831670A4
EP1831670A4 EP05855662A EP05855662A EP1831670A4 EP 1831670 A4 EP1831670 A4 EP 1831670A4 EP 05855662 A EP05855662 A EP 05855662A EP 05855662 A EP05855662 A EP 05855662A EP 1831670 A4 EP1831670 A4 EP 1831670A4
Authority
EP
European Patent Office
Prior art keywords
electrodes
dna sequence
recognition
target dna
polynucleotide sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP05855662A
Other languages
German (de)
French (fr)
Other versions
EP1831670A2 (en
Inventor
Jean-Christophe P Gabriel
Shripal Gandhi
George Gruner
Charles Steven Joiner Jr
Ron Sosnowski
Alexander Star
Eugene Tu
Christian Valcke
Joseph Niemann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanomix Inc
Original Assignee
Nanomix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/212,026 external-priority patent/US20070178477A1/en
Priority claimed from US11/274,747 external-priority patent/US20070208243A1/en
Application filed by Nanomix Inc filed Critical Nanomix Inc
Publication of EP1831670A2 publication Critical patent/EP1831670A2/en
Publication of EP1831670A4 publication Critical patent/EP1831670A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4146—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS involving nanosized elements, e.g. nanotubes, nanowires
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01L—SEMICONDUCTOR DEVICES NOT COVERED BY CLASS H10
    • H01L2924/00—Indexing scheme for arrangements or methods for connecting or disconnecting semiconductor or solid-state bodies as covered by H01L24/00
    • H01L2924/0001—Technical content checked by a classifier
    • H01L2924/0002—Not covered by any one of groups H01L24/00, H01L24/00 and H01L2224/00

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • Nanotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Zoology (AREA)
  • Electrochemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)

Abstract

A nanotube device is configured as an electronic sensor for a target DNA sequence. A film of nanotubes is deposited over electrodes on a substrate. A solution of single-strand DNA is prepared so as to be complementary to a target DNA sequence. The DNA solution is deposited over the electrodes, dried, and removed from the substrate except in a region between the electrodes. The resulting structure includes strands of the desired DNA sequence in direct contact with nanotubes between opposing electrodes, to form a sensor that is electrically responsive to the presence of target DNA strands. Alternative assay embodiments are described which employ linker groups to attach ssDNA probes to the nanotube sensor device.
EP05855662A 2004-12-28 2005-12-23 Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences Withdrawn EP1831670A4 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US63995404P 2004-12-28 2004-12-28
US65727505P 2005-02-28 2005-02-28
US66887905P 2005-04-05 2005-04-05
US11/212,026 US20070178477A1 (en) 2002-01-16 2005-08-24 Nanotube sensor devices for DNA detection
US73090505P 2005-10-27 2005-10-27
US11/274,747 US20070208243A1 (en) 2002-01-16 2005-11-15 Nanoelectronic glucose sensors
US73869405P 2005-11-21 2005-11-21
US74883405P 2005-12-09 2005-12-09
PCT/US2005/047143 WO2006071895A2 (en) 2004-12-28 2005-12-23 Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences

Publications (2)

Publication Number Publication Date
EP1831670A2 EP1831670A2 (en) 2007-09-12
EP1831670A4 true EP1831670A4 (en) 2010-09-15

Family

ID=36615493

Family Applications (1)

Application Number Title Priority Date Filing Date
EP05855662A Withdrawn EP1831670A4 (en) 2004-12-28 2005-12-23 Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences

Country Status (3)

Country Link
EP (1) EP1831670A4 (en)
JP (1) JP2008525822A (en)
WO (1) WO2006071895A2 (en)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8154093B2 (en) 2002-01-16 2012-04-10 Nanomix, Inc. Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices
US7948041B2 (en) 2005-05-19 2011-05-24 Nanomix, Inc. Sensor having a thin-film inhibition layer
JP2008511008A (en) * 2004-08-24 2008-04-10 ナノミックス・インコーポレーテッド Nanotube sensor device for DNA detection
JP2009532064A (en) * 2006-04-04 2009-09-10 ナノミックス・インコーポレーテッド Nanoelectronic detection of biomolecules using analyte amplification and reporter
ES2289948B1 (en) * 2006-07-19 2008-09-16 Starlab Barcelona, S.L. ELECTROPHYSIOLOGICAL SENSOR.
JP5061342B2 (en) * 2006-09-11 2012-10-31 国立大学法人大阪大学 Carbon nanotube electrode and sensor using the electrode
EP2160612A4 (en) * 2007-05-23 2016-04-27 Univ Arizona Systems and methods for integrated electrochemical and electrical detection
CN101939329A (en) * 2007-08-30 2011-01-05 滑铁卢大学 Amino acid pairing-based self assembling peptides and methods
JP5235215B2 (en) * 2008-07-10 2013-07-10 株式会社イデアルスター Gas sensor
AU2009298497B2 (en) 2008-10-02 2013-12-19 12-15 Molecular Diagnostics, Inc. Bionanosensor detection device
GB0822733D0 (en) * 2008-12-12 2009-01-21 Univ Warwick Nanotube electrochemistry
US8993346B2 (en) 2009-08-07 2015-03-31 Nanomix, Inc. Magnetic carbon nanotube based biodetection
JP2011220803A (en) * 2010-04-08 2011-11-04 Mitsumi Electric Co Ltd Biosensor including field-effect transistor element
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
CN103328981B (en) 2010-10-04 2017-04-12 吉纳普赛斯股份有限公司 Systems and methods for automated reusable parallel biological reactions
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
CN103717753B (en) * 2011-05-27 2016-12-07 吉纳普赛斯股份有限公司 For heredity and the system and method for bioanalysis
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
JP6193252B2 (en) 2011-12-01 2017-09-06 ジナプシス インコーポレイテッド System and method for high efficiency electronic sequencing and detection
EP2909614B1 (en) 2012-10-16 2016-12-14 Koninklijke Philips N.V. Integrated circuit with chemfet-sensors and measuring method
CN102938397B (en) * 2012-12-05 2015-09-09 苏州纳格光电科技有限公司 Electronic device and preparation method thereof
WO2014152625A1 (en) 2013-03-15 2014-09-25 Genapsys, Inc. Systems and methods for biological analysis
WO2014191892A1 (en) * 2013-05-29 2014-12-04 Csir A field effect transistor and a gas detector including a plurality of field effect transistors
US10125393B2 (en) 2013-12-11 2018-11-13 Genapsys, Inc. Systems and methods for biological analysis and computation
WO2015086654A1 (en) * 2013-12-12 2015-06-18 Altra Tech Limited A capacitive sensor and method of use
EP3556864B1 (en) 2014-04-18 2020-12-09 Genapsys, Inc. Methods and systems for nucleic acid amplification
US20180133679A1 (en) * 2015-04-15 2018-05-17 Alastair J. Malcolm Methods of manufacturing semiconductor arrays
WO2017033922A1 (en) * 2015-08-24 2017-03-02 国立大学法人 東京大学 Method of detecting target substance using field-effect transistor
CN109790575A (en) 2016-07-20 2019-05-21 吉纳普赛斯股份有限公司 System and method for nucleic acid sequencing
JP2021500858A (en) 2017-09-21 2021-01-14 ジナプシス インコーポレイテッド Systems and methods for nucleic acid sequencing
US20190219512A1 (en) * 2018-01-12 2019-07-18 Clifford H. Kern, III Device, procedure and system for detecting bacterial pathogens including methicillin-resistant staphylococcus aureus or clostridium difficile
CN113588605B (en) * 2021-06-18 2024-08-06 山东师范大学 Method for detecting estrogen in multiple environments and application thereof
US11845079B2 (en) * 2021-12-08 2023-12-19 International Business Machines Corporation Integrated silicon platform for electronic biosensors

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040202603A1 (en) * 1994-12-08 2004-10-14 Hyperion Catalysis International, Inc. Functionalized nanotubes
US7416699B2 (en) * 1998-08-14 2008-08-26 The Board Of Trustees Of The Leland Stanford Junior University Carbon nanotube devices
DE60039632D1 (en) * 1999-12-15 2008-09-04 Univ R CARBON NANO-TUBE DEVICE
KR20090049095A (en) * 2000-12-11 2009-05-15 프레지던트 앤드 펠로우즈 오브 하버드 칼리지 Nanosensors
JP2003322653A (en) * 2002-05-07 2003-11-14 Toshiba Corp Support and carrier for fixing probe
WO2004044586A1 (en) * 2002-11-08 2004-05-27 Nanomix, Inc. Nanotube-based electronic detection of biological molecules
JP2004347532A (en) * 2003-05-23 2004-12-09 Japan Science & Technology Agency Biosensor

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
No further relevant documents disclosed *

Also Published As

Publication number Publication date
WO2006071895A3 (en) 2007-02-08
JP2008525822A (en) 2008-07-17
EP1831670A2 (en) 2007-09-12
WO2006071895A2 (en) 2006-07-06

Similar Documents

Publication Publication Date Title
EP1831670A4 (en) Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences
WO2006024023A3 (en) Nanotube sensor devices for dna detection
Roy et al. Direct electrical measurements on single-molecule genomic DNA using single-walled carbon nanotubes
Liu et al. Electrochemical detection of avian influenza virus H5N1 gene sequence using a DNA aptamer immobilized onto a hybrid nanomaterial-modified electrode
He et al. Transparent, flexible, all-reduced graphene oxide thin film transistors
Gao et al. Silicon-nanowire-based CMOS-compatible field-effect transistor nanosensors for ultrasensitive electrical detection of nucleic acids
EP4023764A3 (en) Binding probe circuits for molecular sensors
MX2016009127A (en) Salivary biosensors and biofuel cells.
EP2691771A1 (en) Aptamer coated measurement and reference electrodes and methods using same for biomarker detection
WO2009049566A3 (en) Device for production of layer of nanofibres through electrostatic spinning of polymer matrices and collecting electrode for such device
WO2008033419A3 (en) Electrochemical sensor with interdigitated microelectrodes and conducted polymer
CN101846648A (en) Electrochemical biosensor modified by graphene quantum dot and preparation method thereof
WO2008106903A3 (en) Device for production of nanofibres and/or nanoparticles from solutions or melts of polymers in electrostatic field
WO2010044932A3 (en) Nanofluidic channels with integrated charge sensors and methods based thereon
ATE500506T1 (en) BIOSENSOR WITH NANOWIRE AND PRODUCTION METHOD THEREOF
SG130107A1 (en) Polymer/nanoparticle composites, film and molecular detection device
WO2014060894A3 (en) Wide dynamic range fluid sensor based on nanowire platform
EP2112580A3 (en) Coordinate detection apparatus
FR2875007B1 (en) BIOLOGICAL CHIP COMPRISING A NETWORK OF ELECTRODES ON A SUBSTRATE
WO2007101223A3 (en) Analyte sensors and methods of use
EP2445006A3 (en) Light-emitting device and method for manufacturing the same
WO2015054663A3 (en) Biomolecular interaction detection devices and methods
WO2011017077A3 (en) Nanochannel-based sensor system with controlled sensitivity
WO2007146369A8 (en) Ammonia gas sensor with dissimilar electrodes
CN105319241A (en) Flexible gas-sensitive sensor and making method thereof

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20070619

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

DAX Request for extension of the european patent (deleted)
RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: NANOMIX, INC.

A4 Supplementary search report drawn up and despatched

Effective date: 20100818

RIC1 Information provided on ipc code assigned before grant

Ipc: C12M 3/00 20060101ALI20100812BHEP

Ipc: G01N 27/00 20060101ALI20100812BHEP

Ipc: G01N 27/12 20060101ALI20100812BHEP

Ipc: G01N 21/00 20060101AFI20070709BHEP

Ipc: G01N 33/543 20060101ALI20100812BHEP

Ipc: C12Q 1/68 20060101ALI20100812BHEP

Ipc: G01N 33/00 20060101ALI20100812BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20110317