EP1749203A4 - Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays - Google Patents

Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays

Info

Publication number
EP1749203A4
EP1749203A4 EP05722991A EP05722991A EP1749203A4 EP 1749203 A4 EP1749203 A4 EP 1749203A4 EP 05722991 A EP05722991 A EP 05722991A EP 05722991 A EP05722991 A EP 05722991A EP 1749203 A4 EP1749203 A4 EP 1749203A4
Authority
EP
European Patent Office
Prior art keywords
real
applications
sensing system
time electronic
cell sensing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP05722991A
Other languages
German (de)
French (fr)
Other versions
EP1749203A2 (en
Inventor
Xiao Xu
Yama Abbassi
Xiaobo Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/987,732 external-priority patent/US7192752B2/en
Application filed by Individual filed Critical Individual
Priority to EP13171137.6A priority Critical patent/EP2639576B1/en
Priority to EP11193882.5A priority patent/EP2450698B1/en
Publication of EP1749203A2 publication Critical patent/EP1749203A2/en
Publication of EP1749203A4 publication Critical patent/EP1749203A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
    • G01N33/4836—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures using multielectrode arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06—Plates; Walls; Drawers; Multilayer plates
    • C12M25/08—Plates; Walls; Drawers; Multilayer plates electrically charged
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/021—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance before and after chemical transformation of the material
EP05722991A 2004-02-09 2005-02-09 Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays Withdrawn EP1749203A4 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP13171137.6A EP2639576B1 (en) 2004-02-09 2005-02-09 Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
EP11193882.5A EP2450698B1 (en) 2004-02-09 2005-02-09 Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US54292704P 2004-02-09 2004-02-09
US54871304P 2004-02-27 2004-02-27
US61460104P 2004-09-29 2004-09-29
US10/987,732 US7192752B2 (en) 2002-12-20 2004-11-12 Real time electronic cell sensing systems and applications for cell-based assays
PCT/US2005/004481 WO2005077104A2 (en) 2004-02-09 2005-02-09 Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP13171137.6A Division EP2639576B1 (en) 2004-02-09 2005-02-09 Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
EP11193882.5A Division EP2450698B1 (en) 2004-02-09 2005-02-09 Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays

Publications (2)

Publication Number Publication Date
EP1749203A2 EP1749203A2 (en) 2007-02-07
EP1749203A4 true EP1749203A4 (en) 2009-05-06

Family

ID=40518464

Family Applications (1)

Application Number Title Priority Date Filing Date
EP05722991A Withdrawn EP1749203A4 (en) 2004-02-09 2005-02-09 Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays

Country Status (4)

Country Link
EP (1) EP1749203A4 (en)
CN (1) CN101400780B (en)
CA (1) CA2556219A1 (en)
WO (1) WO2005077104A2 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7192752B2 (en) 2002-12-20 2007-03-20 Acea Biosciences Real time electronic cell sensing systems and applications for cell-based assays
US7560269B2 (en) 2002-12-20 2009-07-14 Acea Biosciences, Inc. Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
US8263375B2 (en) 2002-12-20 2012-09-11 Acea Biosciences Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology
US10539523B2 (en) 2002-12-20 2020-01-21 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology, and electrophysiological properties
US11346797B2 (en) 2002-12-20 2022-05-31 Agilent Technologies, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology and electrophysiological properties
US10215748B2 (en) 2002-12-20 2019-02-26 Acea Biosciences, Inc. Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways
US10551371B2 (en) 2003-11-10 2020-02-04 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability and morphology and for screening for pharmacological agents which may induce cardiotoxicity or modulate cardiomyocyte function
CA2575573A1 (en) 2004-08-04 2006-02-16 Acea Biosciences, Inc. Dynamic monitoring of activation of g-protein (gpcr) and receptor tyrosine kinase (rtk) in living cells using real-time microelectronic cell sensing technology
PT1977241E (en) 2006-01-04 2010-09-16 Novartis Ag Antibody-dependent cellular cytotoxicity assay
WO2008036375A2 (en) * 2006-09-20 2008-03-27 Acea Biosciences Corporation Use of impedance-based cytological profiling to classify cellular response profiles upon exposure to biologically active agents
ATE518136T1 (en) 2008-02-25 2011-08-15 Univ Leipzig DEVICE AND METHOD FOR MEASURING IMPEDANCE IN ORGANIC TISSUE
EP2291645B1 (en) 2008-05-05 2015-09-09 Acea Biosciences, Inc. Label-free monitoring of excitation-contraction coupling and excitable cells using impedance based systems with millisecond time resolution
US8771202B2 (en) 2010-01-19 2014-07-08 Becton Dickinson And Company Electrode layout for blood test sensor strip
WO2011146531A1 (en) 2010-05-18 2011-11-24 Acea Biosciences, Inc Data analysis of impedance-based cardiomyocyte-beating signals as detected on real-time cell analysis (rtca) cardio instruments
CN103675031B (en) * 2013-12-18 2015-09-02 江苏大学 A kind of high-flux cell detection method of toxicity
EP3035052A1 (en) 2014-12-18 2016-06-22 Helmholtz-Zentrum für Infektionsforschung GmbH Method and measuring device for determination of the growth rate of biofilm
US20180246079A1 (en) * 2015-11-20 2018-08-30 Acea Biosciences, Inc. Cell-substrate impedance monitoring of cancer cells
CN110582569B (en) 2017-03-03 2024-04-02 安捷伦科技有限公司 Methods and systems for functional maturation of iPSC and ESC-derived cardiomyocytes
WO2019028122A1 (en) * 2017-08-01 2019-02-07 Acea Biosciences, Inc. Cell-substrate impedance monitoring of cancer cells of different lineage, origin, or stage
US11733323B2 (en) 2018-06-13 2023-08-22 Centrul International De Biodinamica Systems and methods for measuring cellular response to target analytes by controlled application of an oscillating stimulus
RO133794A2 (en) * 2018-06-13 2019-12-30 Centrul Internaţional De Biodinamică Method and device for detecting bioactive, e.g. cytotoxic, compounds, using sensors with stimulated cells
CN111751522B (en) * 2019-03-27 2023-06-20 中国科学院宁波工业技术研究院慈溪生物医学工程研究所 Cell detection analyzer and detection method of cell proliferation information
CN110568037B (en) * 2019-09-02 2021-12-03 江南大学 Construction method and application of electrochemical cell sensor for acetamiprid and imidacloprid combined toxicity evaluation
CN111735752A (en) * 2020-07-01 2020-10-02 京东方科技集团股份有限公司 Cell detection device and cell detection system
CN112485301B (en) * 2020-11-25 2023-07-14 三诺生物传感股份有限公司 Test method, system, equipment and medium of electrochemical test device

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5187096A (en) * 1991-08-08 1993-02-16 Rensselaer Polytechnic Institute Cell substrate electrical impedance sensor with multiple electrode array
WO2004010103A2 (en) * 2002-07-20 2004-01-29 Acea Biosciences, Inc. Impedance based devices and methods for use in assays
WO2005047482A2 (en) * 2003-11-12 2005-05-26 Xiao Xu Real time electronic cell sensing systems and applications for cell-based assays

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3890201A (en) * 1974-09-26 1975-06-17 Bactomatic Inc Multi-chamber impedance measuring module-cap combination
US4067951A (en) * 1975-11-19 1978-01-10 Bactomatic Inc. Process for making impedance measuring module
JPH11187865A (en) * 1997-12-25 1999-07-13 Matsushita Electric Ind Co Ltd Electrode for measuring cell potential and measuring apparatus by using the same
US6477479B1 (en) * 1998-12-11 2002-11-05 Symyx Technologies Sensor array for rapid materials characterization
IL148650A0 (en) * 1999-10-01 2002-09-12 Sophion Bioscience As Substrate and a method for determining and/or monitoring electrophysiological properties of ion channels
TWI245895B (en) * 2001-11-29 2005-12-21 Sun-Wing Tong Molecular detection and assay by electrobiochip micro-array

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5187096A (en) * 1991-08-08 1993-02-16 Rensselaer Polytechnic Institute Cell substrate electrical impedance sensor with multiple electrode array
WO2004010103A2 (en) * 2002-07-20 2004-01-29 Acea Biosciences, Inc. Impedance based devices and methods for use in assays
WO2005047482A2 (en) * 2003-11-12 2005-05-26 Xiao Xu Real time electronic cell sensing systems and applications for cell-based assays

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
EHRET R ET AL: "Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structures", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 12, no. 1, 1 January 1997 (1997-01-01), pages 29 - 41, XP002272321, ISSN: 0956-5663 *
WOLF B ET AL: "Monitoring of cellular signalling and metabolism with modular sensor-technique: the PhysioControl-Microsystem (PCM)", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 13, no. 5, 15 March 1998 (1998-03-15), pages 501 - 509, XP002408458, ISSN: 0956-5663 *
XIAO C ET AL: "An In-Depth Analysis of Electric Cell-Substrate Impedance Sensing to Study the Attachment and Spreading of Mammalian Cells", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 74, no. 6, 15 March 2002 (2002-03-15), pages 1333 - 1339, XP002977290, ISSN: 0003-2700 *

Also Published As

Publication number Publication date
CN101400780A (en) 2009-04-01
CA2556219A1 (en) 2005-08-25
EP1749203A2 (en) 2007-02-07
WO2005077104A2 (en) 2005-08-25
CN101400780B (en) 2012-06-20
WO2005077104A3 (en) 2008-08-14

Similar Documents

Publication Publication Date Title
EP1749203A4 (en) Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
TWI315570B (en) Electronic package
EP1729933A4 (en) Electronic fastening tool
GB0506908D0 (en) Electronic trading systems
EP1733243A4 (en) Power line sensors and systems incorporating same
EP1855315A4 (en) Electronic tag chip
IL186425A0 (en) Stilbazium research assays
GB0423742D0 (en) Electronic trading system
GB2431519B (en) Superconducting systems
SG120217A1 (en) Die anti-tampering sensor
GB2427628B (en) Improvements in or relating to wall systems
GB0525449D0 (en) Forming Electronic Devices
EP1942337A4 (en) Sensor chip and sensor system
GB0617745D0 (en) Analysing electronic devices
GB2424286B (en) Tamper proof utility metering
GB2388881B (en) Threaded bolt fastener
GB0503318D0 (en) Testing electronic systems
GB0402435D0 (en) Electronic trading system
EP1988094A4 (en) Hapten compound and antibody
SG122043A1 (en) Ligand-receptor tracking assays
GB0508578D0 (en) Measuring systems and devices
GB0511005D0 (en) Loose-proof screw bolt
GB0413780D0 (en) Electronic sensor
GB2417537B (en) Improvements relating to threaded fastening systems
GB0414044D0 (en) Electronic distance measurement system

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20060906

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL BA HR LV MK YU

DAX Request for extension of the european patent (deleted)
PUAK Availability of information related to the publication of the international search report

Free format text: ORIGINAL CODE: 0009015

A4 Supplementary search report drawn up and despatched

Effective date: 20090403

17Q First examination report despatched

Effective date: 20110608

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20111220