EP1723248A2 - Procedes et systemes servant a produire de l'ethanol au moyen d'amidon brut et d'un fractionnement - Google Patents
Procedes et systemes servant a produire de l'ethanol au moyen d'amidon brut et d'un fractionnementInfo
- Publication number
- EP1723248A2 EP1723248A2 EP05725366A EP05725366A EP1723248A2 EP 1723248 A2 EP1723248 A2 EP 1723248A2 EP 05725366 A EP05725366 A EP 05725366A EP 05725366 A EP05725366 A EP 05725366A EP 1723248 A2 EP1723248 A2 EP 1723248A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- fermenting
- plant material
- saccharifying
- fermentation
- ethanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Definitions
- the present invention relates to methods for producing high levels of alcohol during fe ⁇ nentation of plant material, and to the high alcohol beer produced.
- the method can include fractionating the plant material.
- the present invention also relates to methods for producing high protein distiller's dried grain from fermentation of plant material, and to the high protein distiller's dried grain produced.
- the method can include drying a co-product by ring drying, flash drying, or fluid bed drying.
- the present invention further relates to reduced stack emissions from drying distillation products from the production of ethanol.
- the present invention relates to methods for producing high levels of alcohol during fermentation of plant material, and to the high alcohol beer produced.
- the method can include fractionating the plant material.
- the present invention also relates to methods for producing high protein distiller's dried grain from fermentation of plant material, and to the high protein distiller's dried grain produced.
- the method can include drying a co-product by ring drying, flash drying, or fluid bed drying.
- the present invention relates to a process for producing ethanol from plant material (e.g., fractionated plant material).
- This method includes fractionating the plant material; grinding the plant material (e.g., fractionated plant material) to produce ground plant material (e.g., fractionated plant material) including starch; saccharifying the starch, without cooking; fermenting the incubated starch; and recovering the ethanol from the fermentation.
- the present method can include varying the temperature during fermentation.
- the present method can include employing plant material (e.g., fractionated plant material) with a particle size such that more than 50% of the material fits though a sieve with a 0.5 mm mesh.
- the present method can yield a composition including at least 18 vol-% ethanol.
- the present invention relates to a process for producing high protein distiller's dried grain from plant material (e.g., fractionated plant material).
- This method includes fractionating the plant material; grinding the plant material (e.g., fractionated plant material) to produce ground plant material (e.g., fractionated plant material) including starch; producing sugars from the starch without cooking; fermenting the uncooked sugars to yield a composition including ethanol; and recovering distiller's dried grain from the fermentation.
- the distiller's dried grain can include at least about 30% protein.
- the distillers dried grain can include increased levels of the protein zein.
- the present invention relates to a process of producing ethanol from corn. This process includes producing starch from corn and ethanol from the starch; producing dryer stack emissions including a significantly lower level of volatile organic compounds than conventional technologies.
- Figure 1 schematically illustrates a fermentation system according to an embodiment of the present invention.
- Figures 2A through 2C schematically illustrate that the present process provides improved efficiency for fermentation of com fractions produced by dry milling fractionation processes.
- Figures 3 A through 3C schematically illustrate that the present process provides improved efficiency for fermentation of corn fractions produced by dry milling fractionation processes.
- without cooking refers to a process for converting starch to ethanol without heat treatment for gelatinization and dextrinization of starch using alpha-amylase.
- without cooking refers to maintaining a temperature below starch gelatinization temperatures, so that saccharification occurs directly from the raw native insoluble starch to soluble glucose while bypassing conventional starch gelatinization conditions.
- Starch gelatinization temperatures are typically in a range of 57 °C to 93 °C depending on the starch source and polymer type.
- dextrinization of starch using conventional liquefaction techniques is not necessary for efficient fermentation of the carbohydrate in the grain.
- plant material refers to all or part of any plant (e.g., cereal grain), typically a material including starch.
- Suitable plant material includes grains such as maize (com, e.g., whole ground com), sorghum (milo), barley, wheat, rye, rice, and millet; and starchy root crops, tubers, or roots such as sweet potato and cassava.
- the plant material can be a mixture of such materials and byproducts of such materials, e.g., com fiber, com cobs, stover, or other cellulose and hemicellulose containing materials such as wood or plant residues.
- Suitable plant materials include com, either standard com or waxy com.
- fractionated plant material refers to plant material that includes only a portion or fraction of the total plant material, typically a material including starch.
- Fractionated plant material can include fractionated grains such as fractionated maize (fractionated com), fractionated sorghum (fractionated milo), fractionated barley, fractionated wheat, fractionated rye, fractionated rice, and fractionated millet; and fractionated starchy root crops, tubers, or roots such as fractionated sweet potato and fractionated cassava.
- Suitable fractionated plant materials include fractionated com, either fractionated standard com or fractionated waxy com.
- saccharification and “saccharifying” refer to the process of converting starch to smaller polysaccharides and eventually to monosaccharides, such as glucose.
- Conventional saccharification uses liquefaction of gelatinized starch to create soluble dextrinized substrate which glucoamylase enzyme hydrolyzes to glucose.
- saccharification refers to converting raw starch to glucose with enzymes, e.g., glucoamylase and acid fungal amylase (AFAU).
- AFAU acid fungal amylase
- the raw starch is not subjected to conventional liquefaction and gelatinization to create a conventional dextrinized substrate.
- a unit of acid fungal amylase activity refers to the standard Novozymes units for measuring acid fungal amylase activity.
- the Novozymes units are described in a Novozymes technical bulletin SOP No. : EB-SM-0259.02/01. Such units can be measured by detecting products of starch degradation by iodine titration. 1 unit is defined as the amount of enzyme that degrades 5.260 mg starch dry matter per hour under standard conditions.
- a unit of glucoamylase activity refers to the standard Novozymes units for measuring glucoamylase activity.
- the Novozymes units and assays for determining glucoamylase activity are described in a publicly available Novozymes technical bulletin.
- a unit of amyloglucosidase activity refers to the standard Novozymes units for measuring amyloglucosidase activity.
- the Novozymes units are described in a Novozymes technical bulletin SOP No. : EB-SM-0131.02/01. Such units can be measured by detecting conversion of maltose to glucose.
- the glucose can be determined using the glucose dehydrogenase reaction.
- 1 unit is defined as the amount of enzyme that catalyzes the conversion of 1 mmol maltose per minute under the given conditions.
- the term "about” modifying any amount refers to the variation in that amount encountered in real world conditions of producing sugars and ethanol, e.g., in the lab, pilot plant, or production facility.
- an amount of an ingredient employed in a mixture when modified by “about” includes the variation and degree of care typically employed in measuring in an ethanol production plant or lab.
- the amount of a component of a product when modified by “about” includes the variation between batches in an ethanol production plant or lab and the variation inherent in the analytical method. Whether or not modified by “about,” the amounts include equivalents to those amounts. Any quantity stated herein and modified by “about” can also be employed in the present invention as the amount not modified by "about.”
- the present invention relates to methods for producing high levels of alcohol during fermentation of plant material (e.g., fractionated plant material), and to the high alcohol beer produced.
- the present invention also relates to methods for producing high protein distiller's dried grain from fermentation of plant material (e.g., fractionated plant material), to the high protein distiller's dried grain produced, and to the cleaner dryer stack emissions.
- the present method converts starch from plant material (e.g., fractionated plant material) to ethanol.
- the present method can include preparing the plant material (e.g., fractionated plant material) for saccharification, converting the prepared plant material (e.g., fractionated plant material) to sugars without cooking, and fermenting the sugars.
- the plant material (e.g., fractionated plant material) can be prepared for saccharification by any a variety of methods, e.g., by grinding, to make the starch available for saccharification and fermentation.
- the vegetable material can be ground so that a substantial portion, e.g., a majority, of the ground material fits through a sieve with a 0.1-0.5 mm screen.
- a substantial portion e.g., a majority
- the ground vegetable material can fit through a sieve with a 0.1- 0.5 mm screen.
- about 70% or more, of the ground vegetable material can fit through a sieve with a 0.1- 0.5 mm screen.
- the reduced plant material e.g., fractionated plant material
- liquid at about 20 to about 50 wt-% or about 25 to about 45 wt-% dry reduced plant material (e.g., fractionated plant material).
- the present process can include converting reduced plant material (e.g., fractionated plant material) to sugars that can be fermented by a microorganism such as yeast. This conversion can be effected by saccharifying the reduced plant material (e.g., fractionated plant material) with an enzyme preparation, such as a saccharifying enzyme composition.
- a saccharifying enzyme composition can include any of a variety of known enzymes suitable for converting reduced plant material (e.g., fractionated plant material) to fermentable sugars, such as amylases (e.g., ⁇ -amylase and/or glucoamylase).
- saccharification is conducted at a pH of about 6.0 or less, for example, about 4.5 to about 5.0, for example, about 4.5 to about 4.8.
- the present process includes fermenting sugars from reduced plant material
- fermentation can be effected by a microorganism, such as yeast.
- fermentation is conducted at a pH of about 6 or less, for example, about 4.5 to about 5, for example, about 4.5 to about 4.8.
- the present method can include varying the pH.
- fermentation can include filling the fermenter at pH of about 3 to about 4.5 during the first half of fill and at a pH of about 4.5 to about 6 (e.g., about 4.5 to about 4.8) during the second half of the fermenter fill cycle.
- fermentation is conducted at a temperature of about 25 to about 40 °C or about 30 to about 35 °C.
- the temperature is decreased from about 40 °C to about 30 °C or about 25 °C, or from about 35 °C to about 30 °C, during the first half of the fermentation, and the temperature is held at the lower temperature for the second half of the fermentation.
- fermentation is conducted for about 25 (e.g., 24) to about to 150 hours, for example, for about 48 (e.g., 47) to about 96 hours.
- the present process can include simultaneously converting reduced plant material (e.g., fractionated plant material) to sugars and fermenting those sugars with a microorganism such as yeast.
- the product of the fermentation process is referred to herein as "beer".
- Ethanol can be recovered from the fermentation mixture, from the beer, by any of a variety of known processes, such as by distilling.
- the remaining stillage includes both liquid and solid material.
- the liquid and solid can be separated by, for example, centrifugation.
- Preparing the Plant Material converts starch from plant material (e.g., fractionated plant material) to ethanol.
- the plant material e.g., fractionated plant material
- the plant material e.g., fractionated plant material
- vegetable material such as kernels of com
- the use of emulsion technology, rotary pulsation, and other means of particle size reduction can be employed to increase surface area of plant material (e.g., fractionated plant material) while raising the effectiveness of flowing the liquefied media.
- the prepared plant material e.g., fractionated plant material
- the prepared plant material can be refe ⁇ ed to as being or including "raw starch".
- a fine grind exposes more surface area of the plant material (e.g., fractionated plant material), or vegetable material, and can facilitate saccharification and fermentation.
- the vegetable material is ground so that a substantial portion, e.g., a majority, of the ground material fits through a sieve with a 0.1-0.5 mm screen. In an embodiment, about 35% or more of the ground vegetable material can fit through a sieve with a 0.1-0.5 mm screen. In an embodiment, about 35 to about 70% of the ground vegetable material can fit through a sieve with a 0.1-0.5 mm screen. In an embodiment, about 50% or more of the ground vegetable material can fit through a sieve with a 0.1-0.5 mm screen. In an embodiment, about 90% of the ground vegetable material can fit through a sieve with a 0.1-0.5 mm screen. In an embodiment, all of the ground vegetable material can fit through a sieve with a 0.1-0.5 mm screen. In an embodiment, the ground vegetable material has an average particle size of about 0.25 mm.
- Plant Material Reduction Preparing the plant material (e.g., fractionated plant material) can employ any of a variety of techniques for plant material (e.g., fractionated plant material) reduction.
- the present method of preparing plant material can employ emulsion technology, rotary pulsation, sonication, magnetostriction, ferromagnetic materials, or the like. These methods of plant material reduction can be employed for substrate pretreatment. Although not limiting to the present invention, it is believed that these methods can increase surface area of plant material (e.g., fractionated plant material) while raising the effectiveness of flowing of liquefied media (i.e. decreased viscosity).
- These methods can include electrical to mechanical, mechanical to electrical, pulse, and sound based vibrations at varying speeds. This can provide varying frequencies over a wide range of frequencies, which can be effective for pretreating the plant material (e.g., fractionated plant material) and/or reducing particle size.
- plant material e.g., fractionated plant material
- reducing particle size e.g., reducing particle size.
- certain of these sonic methods create low pressure around a particle of plant material (e.g., fractionated plant material) and induce cavitation of the particle or disruption of the particle structure.
- the cavitated or disrupted particle can increase availability of plant material (e.g., starch) to an enzyme, for example, by increasing surface area. It is believed that such pretreatment can decrease quantity of enzyme rates in the present method for ethanol production.
- the present method includes vibrating plant material (e.g., fractionated plant material) and cavitating the fluid containing the plant material. This can result in dismpting the plant material and/or decreasing the size of the plant material (e.g., fractionated plant material).
- the present method includes treating plant material (e.g., fractionated plant material) with emulsion technology, with rotary pulsation, with magnetostriction, or with ferromagnetic materials. This can result in disrupting the plant material and/or decreasing the size of the plant material (e.g., fractionated plant material).
- the present method includes sonicating the plant material (e.g., fractionated plant material).
- the present method can include employing sound waves for reducing plant material (e.g., fractionated plant material).
- the sound waves can be ultrasound.
- the present method can include sonicating the plant material (e.g., fractionated plant material).
- the method can include sonicating the plant material at a frequency (e.g., measured in kHz), power (e.g., measured in watts), and for a time effective to reduce (or to assist in reducing) the particle size to sizes described hereinabove.
- the method can include sonicating the plant material (e.g., fractionated plant material) at 20,000 Hz and up to about 3000 W for a sufficient time and at a suitable temperature.
- sonicating can be carried out with commercially available apparatus, such as high powered ultrasonics available from ETREMA (Ames, LA).
- the present method can include employing rotary pulsation for reducing plant material (e.g., fractionated plant material).
- the method can include rotary pulsating the plant material (e.g., fractionated plant material) at a frequency (e.g., measured in Hz), power (e.g., measured in watts), and for a time effective to reduce (or to assist in reducing) the particle size to sizes described hereinabove.
- rotary pulsating can be carried out with known apparatus, such as apparatus described in U.S. Patent No. 6,648,500, the disclosure of which is incorporated herein by reference.
- the present method can include employing pulse wave technology for reducing plant material (e.g., fractionated plant material).
- the method can include rotary pulsing the plant material at a frequency (e.g., measured in Hz), power (e.g., measured in watts), and for a time effective to reduce (or to assist in reducing) the particle size to sizes described hereinabove.
- a frequency e.g., measured in Hz
- power e.g., measured in watts
- Such pulsing can be carried out with known apparatus, such as apparatus described in U.S. Patent No. 6,726,133, the disclosure of which is incorporated herein by reference.
- the vegetable material can be fractionated into one or more components.
- a vegetable material such as a cereal grain or com can be fractionated into components such as fiber (e.g., com fiber), germ (e.g., com germ), and a mixture of starch and protein (e.g., a mixture of com starch and com protein).
- fiber e.g., com fiber
- germ e.g., com germ
- starch and protein e.g., a mixture of com starch and com protein
- One or a mixture of these components can be fermented in a process according to the present invention.
- Fractionation of com or another plant material can be accomplished by any of a variety of methods or apparatus.
- a system manufactured by Satake can be used to fractionate plant material such as com.
- the germ and fiber components of the vegetable material can be fractionated and separated from the remaining portion of the vegetable material.
- the remaining portion of the vegetable material can be further milled and reduced in particle size and then combined with the larger pieces of the fractioned germ and fiber components for fermenting.
- the vegetable material can be milled to access value added products (such as neutraceuticals, leutein, carotenoids, xanthrophils, pectin, cellulose, lignin, mannose, xylose, arabinose, galactose, galacturonic acid, GAB A, com oil, albumins, globulins, prolamins, gluetelins, zein and the like).
- Fractionation can be accomplished by any of a variety of methods and apparatus, such as those disclosed in U.S. Patent Application Publication No. 2004/0043117, the disclosure of which is incorporated herein by reference. Suitable methods and apparatus for fractionation include a sieve, sieving, and elutriation.
- Suitable apparatus include a frictional mill such as a rice or grain polishing mill (e.g., those manufactured by Satake, Kett, or Rapsco)
- a frictional mill such as a rice or grain polishing mill (e.g., those manufactured by Satake, Kett, or Rapsco)
- Saccharification and Fermentation Saccharification The present process can include converting reduced plant material (e.g., fractionated plant material) to sugars that can be fermented by a microorganism such as yeast.
- This conversion can be effected by saccharifying the reduced plant material (e.g., fractionated plant material) with any of a variety of known saccharifying enzyme compositions.
- the saccharifying enzyme composition includes an amylase, such as an alpha amylase (e.g., an acid fungal amylase).
- the enzyme preparation can also include glucoamylase.
- the enzyme preparation need not, and, in an embodiment, does not include protease.
- ethanol production methods according to the present invention can conserve water by reusing process waters (backset) which may contain protease.
- the present method employs acid fungal amylase for hydrolyzing raw starch.
- Saccharifying can be conducted without cooking.
- saccharifying can be conducted by mixing source of saccharifying enzyme composition (e.g., commercial enzyme), yeast, and fermentation ingredients with ground grain and process waters without cooking.
- saccharifying can include mixing the reduced plant material
- the method includes mixing the reduced plant material (e.g., fractionated plant material) and liquid and then adding the saccharifying enzyme composition.
- adding enzyme composition can precede or occur simultaneously with mixing.
- the reduced plant material e.g., fractionated plant material
- the reduced plant material can be mixed with liquid at about 20 to about 50 wt-%, about 25 to about 45 (e.g., 44) wt-%, about 30 to about 40 (e.g., 39) wt-%, or about 35 wt-% dry reduced plant material (e.g., fractionated plant material).
- wt-% of reduced plant material in a liquid refers to the percentage of dry substance reduced plant material or dry solids.
- the method of the present invention can convert raw or native starch (e.g., in dry reduced plant material) to ethanol at a faster rate at higher dry solids levels compared to conventional saccharification with cooking.
- the present method can be practiced at higher dry solids levels because, unlike the conventional process, it does not include gelatinization, which increases viscosity.
- Suitable liquids include water and a mixture of water and process waters, such as stillage (backset), scrubber water, evaporator condensate or distillate, side stripper water from distillation, or other ethanol plant process waters.
- the liquid includes water. In an embodiment, the liquid includes water in a mixture with about 1 to about 70 vol-% stillage, about 15 to about 60 vol-% stillage, about 30 to about 50 vol-% stillage, or about 40 vol-% stillage.
- stillage provides nutrients for efficient yeast fermentation, especially free amino nitrogen (FAN) required by yeast.
- FAN free amino nitrogen
- the present invention can provide effective fermentation with reduced levels of stillage and even without added stillage.
- the present method employs a preparation of plant material (e.g., fractionated plant material) that supplies sufficient quantity and quality of nitrogen for efficient fermentation under high gravity conditions (e.g., in the presence of high levels of reduced plant material).
- the present method provides the flexibility to employ high levels of stillage if desired.
- the present method does not employ conventional liquefaction.
- Conventional liquefaction increases viscosity of the fermentation mixture and the resulting stillage.
- the present method produces lower viscosity stillage. Therefore, in an embodiment, increased levels of stillage can be employed in the present method without detrimental increases in viscosity of the fermentation mixture or resulting stillage.
- conventional saccharification and fermentation processes require added FAN due to undesirable "Maillard Reactions" which occur during high temperature gelatinization and liquefaction. The Maillard Reactions consume FAN during cooking.
- Saccharification can employ any of a variety of known enzyme sources (e.g., a microorganism) or compositions to produce fermentable sugars from the reduced plant material (e.g., fractionated plant material).
- the saccharifying enzyme composition includes an amylase, such as an alpha amylase (e.g., an acid fungal amylase) or a glucoamylase.
- saccharification is conducted at a pH of about 6.0 or less, pH of about 3.0 to about 6.0, about 3.5 to about 6.0, about 4.0 to about 5.0, about 4.0 to about 4.5, about 4.5 to about 5.0, or about 4.5 to about 4.8.
- saccharification is conducted at a pH of about 4.1 to about 4.6 or about 4.9 to about 5.3.
- the initial pH of the saccharification mixture can be adjusted by addition of, for example, ammonia, sulfuric acid, phosphoric acid, process waters (e.g., stillage (backset), evaporator condensate (distillate), side stripper bottoms, and the like), and the like.
- saccharifying enzyme compositions e.g., one including acid fungal amylase
- saccharification is conducted at a temperature of about 25 to 5 about 40 °C or about 30 to about 35 °C.
- saccharifying can be carried out employing quantities of saccharifying enzyme composition selected to maintain low concentrations of dextrin in the fermentation broth.
- the present process can employ quantities of saccharifying enzyme composition selected to maintain maltotriose (DP3) at levels at or0 below about 0.2 wt-% or at or below about 0.1 wt-%.
- DP3 maltotriose
- the present process can employ quantities of saccharifying enzyme composition selected to maintain dextrin with a degree of polymerization of 4 or more (DP4+) at levels at or below about 1 wt-% or at or below about 0.5 wt-%.
- saccharifying can be carried out employing quantities of5 saccharifying enzyme composition selected to maintain low concentrations of maltose in the fermentation broth.
- the present process can employ quantities of saccharifying enzyme composition selected to maintain maltose at levels at or below about 0.3 wt-%.
- suitable levels of acid fungal amylase and glucoamylase include about 0.05 to about 3 AFAU/ gram dry solids0 reduced plant material (e.g., DSC) of acid fungal amylase and about 1 to about 2.5 (e.g., 2.4) AGU per gram dry solids reduced plant material (e.g., DSC) of glucoamylase.
- the reaction mixture includes about 0.1 to about 2 AFAU/ gram dry solids reduced plant material (e.g., DSC) of acid fungal amylase and about 1 to about 2.5 AGU per gram dry solids reduced plant material (e.g., DSC) of glucoamylase.
- the reaction mixture includes about 0.3 to about 2 AFAU/ gram dry solids reduced plant material (e.g., DSC) of acid fungal amylase and about 1 to about 2.5
- the reaction mixture includes about 1 to about 2 AFAU/ gram dry solids reduced plant material (e.g., DSC) of acid fungal amylase and about 1 to about 1.50 AGU per gram dry solids reduced plant material (e.g., DSC) of glucoamylase.
- Glucoamylase In certain embodiments, the present method can employ a glucoamylase. Glucoamylase is also known as amyloglucosidase and has the systematic name 1,4- alpha-D-glucan glucohydrolase (E.G. 3.2.1.3).
- Glucoamylase refers to an enzyme that removes successive glucose units from the non-reducing ends of starch.
- certain glucoamylases can hydrolyze both the linear and branched glucosidic linkages of starch, amylose, and amylopectin.
- suitable glucoamylases are known and commercially available.
- suppliers such as Novozymes and Genencor provide glucoamylases.
- the glucoamylase can be of fungal origin.
- the amount of glucoamylase employed in the present process can vary according to the enzymatic activity of the amylase preparation.
- Suitable amounts include about 0.05 to about 6.0 glucoamylase units (AGU) per gram dry solids reduced plant material (e.g., DSC).
- the reaction mixture can include about 1 to about 6 AGU per gram dry solids reduced plant material (e.g., DSC).
- the reaction mixture can include about 1 to about 3 AGU per gram dry solids reduced plant material (e.g., DSC).
- the reaction mixture can include about 1 to about 2.5 (e.g., 2.4) AGU per gram dry solids reduced plant material (e.g., DSC).
- the reaction mixture can include about 1 to about 2 AGU per gram dry solids reduced plant material (e.g., DSC).
- the reaction mixture can include about 1 to about 1.5 AGU per gram dry solids reduced plant material (e.g., DSC). In an embodiment, the reaction mixture can include about 1.2 to about 1.5 AGU per gram dry solids reduced plant material (e.g., DSC).
- the present method employs an ⁇ -amylase.
- the - amylase can be one produced by fungi.
- the ⁇ -amylase can be one characterized by its ability to hydrolyze carbohydrates under acidic conditions.
- An amylase produced by fungi and able to hydrolyze carbohydrates under acidic conditions is referred to herein as acid fungal amylase, and is also known as an acid stable fungal ⁇ -amylase.
- Acid fungal amylase can catalyze the hydrolysis of partially hydrolyzed starch and large oligosaccharides to sugars such as glucose.
- the acid fungal amylase that can be employed in the present process can be characterized by its ability to aid the hydrolysis of raw or native starch, enhancing the saccharification provided by glucoamylase.
- the acid fungal amylase produces more maltose than conventional (e.g., bacterial) ⁇ -amylases.
- Suitable acid fungal amylase can be isolated from any of a variety of fungal species, including Aspergillus, Rhizopus, Mucor, Candida, Coriolus, Endothia, Enthomophtora, Irpex, Penicillium, Sclerotium and Torulopsis species.
- the acid fungal amylase is thermally stable and is isolated from Aspergillus species, such as A.
- the acid fungal amylase is isolated from Aspergillus niger.
- the acid fungal amylase activity can be supplied as an activity in a glucoamylase preparation, or it can be added as a separate enzyme.
- a suitable acid fungal amylase can be obtained from Novozymes, for example in combination with glucoamylase.
- the amount of acid fungal amylase employed in the present process can vary according to the enzymatic activity of the amylase preparation.
- Suitable amounts include about 0.1 to about 10 acid fungal amylase units (AFAU) per gram of dry solids reduced plant material (e.g., dry solids com (DSC)).
- the reaction mixture can include about 0.05 to about 3 AFAU/ gram dry solids reduced plant material (e.g., DSC).
- the reaction mixture can include about 0.1 to about 3 AFAU/ gram dry solids reduced plant material (e.g., DSC).
- the reaction mixture can include about 0.3 to about 3 AFAU/ gram dry solids reduced plant material (e.g., DSC).
- the reaction mixture can include about 1 to about 2 AFAU/ gram dry solids reduced plant material (e.g., DSC).
- Fermenting The present process includes fermenting sugars from reduced plant material (e.g., fractionated plant material) to ethanol. Fermenting can be effected by a microorganism, such as yeast.
- the fermentation mixture need not, and in an embodiment does not, include protease. However, the process waters may contain protease. The amount of protease can be less than that used in the conventional process.
- fermenting is conducted on a starch composition that has not been cooked, h an embodiment, the present fermentation process produces potable alcohol. Potable alcohol has only acceptable, nontoxic levels of other alcohols, such as fusel oils.
- Fermenting can include contacting a mixture including sugars from the reduced plant material (e.g., fractionated plant material) with yeast under conditions suitable for growth of the yeast and production of ethanol.
- fermenting employs the saccharification mixture.
- Any of a variety of yeasts can be employed as the yeast starter in the present process.
- Suitable yeasts include any of a variety of commercially available yeasts, such as commercial strains of Saccharomyces cerevisiae. Suitable strains include "Fali” (Fleischmann's), Thermosac (Alltech), Ethanol Red (LeSafre), BioFerm AFT (North American Bioproducts), and the like.
- the yeast is selected to provide rapid growth and fermentation rates in the presence of high temperature and high ethanol levels.
- Fali yeast has been found to provide good performance as measured by final alcohol content of greater than 17% by volume.
- the amount of yeast starter employed is selected to effectively produce a commercially significant quantity of ethanol in a suitable time, e.g., less than 75 hours.
- yeast starter can be added to the fermentation by any of a variety of methods known for adding yeast to fermentation processes. For example, yeast starter can be added as a dry batch, or by conditioning/propagating. In an embodiment, yeast starter is added as a single inoculation.
- yeast is added to the fermentation during the fermenter fill at a rate of 5 to 100 pounds of active dry yeast (ADY) per 100,000 gallons of fermentation mash.
- the yeast can be acclimated or conditioned by incubating about 5 to 50 pounds of ADY per 10,000 gallon volume of fermenter volume in a prefermenter or propagation tank. Incubation can be from 8 to 16 hours during the propagation stage, which is also aerated to encourage yeast growth.
- the prefermenter used to inoculate the main fermenter can be from 1 to 10% by volume capacity of the main fermenter, for example, from 2.5 to 5% by volume capacity relative to the main fermenter.
- the fermentation is conducted at a pH of about 6 or less, pH of about 3 to about 6, about 3 to about 4.5, about 3.5 to about 6, about 4 to about 5, about 4 to about 4.5, about 4.5 to about 5, or about 4.5 to about 4.8.
- the initial pH of the fermentation mixture can be adjusted by addition of, for example, ammonia, sulfuric acid, phosphoric acid, process waters (e.g., stillage (backset), evaporator condensate (distillate), side stripper bottoms, and the like), and the like.
- process waters e.g., stillage (backset), evaporator condensate (distillate), side stripper bottoms, and the like
- distillery yeast grow well over the pH range of 3 to 6, but are more tolerant of lower pH's down to 3.0 than most contaminant bacterial strains.
- the present method can include varying the pH. It is believed that varying the pH can be conducted to reduce the likelihood of contamination early in fermentation and/or to increase yeast growth and fermentation during the latter stages of fermentation.
- fermentation can include filling the fermenter at pH of about 3 to about 4.5 during the first half of fill.
- Fermentation can include increasing the slurry pH to pH of about 4.5 to about 6 during the second half of the fermenter fill cycle.
- Fermentation can include maintaining pH by adding fresh substrate slurry at the desired pH as described above.
- pH is not adjusted. Rather, in this embodiment, the pH is determined by the pH of the components during filling. In an embodiment, the pH is decreased to about five (5) or below in the com process waters. In an embodiment, the pH is about pH 4 (e.g. 4.1) at the start of fermentation fill and is increased to about pH 5 (e.g. 5.2) toward the end of fermentation fill. In an embodiment, the method includes stopping pH control of the mash slurry after the yeast culture becomes established during the initial process of filling the fermenter, and then allowing the pH to drift up in the com process waters during the end stages of filling the fermenter.
- the method includes stopping pH control of the mash slurry after the yeast culture becomes established during the initial process of filling the fermenter, and then allowing the pH to drift up in the com process waters during the end stages of filling the fermenter.
- fermentation is conducted for about to 25 (e.g., 24) to about to 150 hours, about 25 (e.g., 24) to about 96 hours, about 40 to about 96 hours, about 45 (e.g., 44) to about 96 hours, about 48 (e.g., 47) to about 96 hours.
- fermentation can be conducted for about 30, about 40, about 50, about 60, or about 70 hours.
- fermentation can be conducted for about 35, about 45, about 55, about 65, or about 75 hours.
- fermentation is conducted at a temperature of about 25 to about 40 °C or about 30 to about 35 °C.
- the temperature is decreased from about 40 °C to about 30 °C or about 25 °C, or from about 35 °C to about 30 °C, during the first half of the fermentation, and the temperature is held at the lower temperature for the second half of the fermentation.
- the temperature can be decreased as ethanol is produced.
- the temperature can be as high as about 99 °F and then reduced to about 79 °F. This temperature reduction can be coordinated with increased ethanol titers (%) in the fermenter.
- the present method includes solids staging. Solids staging includes filling at a disproportionately higher level of solids during the initial phase of the fermenter fill cycle to increase initial fermentation rates.
- the solids concentration of the mash entering the fermenter can then be decreased as ethanol titers increase and/or as the fermenter fill cycle nears completion.
- the solids concentration can be about 40% (e.g. 41%) during the first half of the fermentation fill. This can be decreased to about 25% after the fermenter is 50% full and continuing until the fermenter fill cycle is concluded.
- such a strategy results in a full fermenter with solids at 33%. It is believed that solids staging can accelerate enzyme hydrolysis rates and encourage a rapid onset to fermentation by using higher initial fill solids. It is believed that lowering solids in the last half of fill can reduce osmotic pressure related stress effects on the yeast. By maintaining overall fermenter fill solids within a specified range of fermentability, solids staging improves the capacity of the yeast to ferment high gravity mashes toward the end of fermentation.
- Simultaneous Saccharification and Fermentation can include simultaneously converting reduced plant material (e.g., fractionated plant material) to sugars and fermenting those sugars with a microorganism such as yeast.
- Simultaneous saccharifying and fermenting can be conducted using the reagents and conditions described above for saccharifying and fermenting.
- saccharification and fermentation is conducted at a temperature of about 25 to about 40 °C or about 30 to about 35 °C.
- the temperature is decreased from about 40 to about 25 °C or from about 35 to about 30 °C during the first half of the saccharification, and the temperature is held at the lower temperature for the second half of the saccharification.
- higher temperatures early during saccharification and fermentation can increase conversion of starch to fermentable sugar when ethanol concentrations are low. This can aid in increasing ethanol yield. At higher ethanol concentrations, this alcohol can adversely affect the yeast.
- lower temperatures later during saccharification and fermentation are beneficial to decrease stress on the yeast. This can aid in increasing ethanol yield.
- higher temperatures early during saccharification and fermentation can reduce viscosity during at least a portion of the fermentation. This can aid in temperature control. It is also believed that lower temperatures later during saccharification and fermentation are beneficial to reduce the formation of glucose after the yeast has stopped fermenting.
- saccharification and fermentation is conducted at a pH of about 6 or less, pH of about 3 to about 6, about 3.5 to about 6, about 4 to about 5, about 4 to about 4.5, about 4.5 to about 5, or about 4.5 to about 4.8.
- the initial pH of the saccharification and fermentation mixture can be adjusted by addition of, for example, ammonia, sulfuric acid, phosphoric acid, process waters (e.g., stillage (backset), evaporator condensate (distillate), side stripper bottoms, and the like), and the like.
- saccharification and fermentation is conducted for about to 25 (e.g., 24) to about to 150 hours, about 25 (e.g., 24) to about 72 hours, about 45 to about 55 hours, about 50 (e.g., 48) to about 96 hours, about 50 to about 75 hours, or about 60 to about 70 hours.
- saccharification and fermentation can be conducted for about 30, about 40, about 50, about 60, or about 70 hours.
- saccharification and fermentation can be conducted for about 35, about 45, about 55, about 65, or about 75 hours.
- simultaneous saccharifying and fermenting can be carried out employing quantities of enzyme and yeast selected to maintain high concentrations of yeast and high levels of budding of the yeast in the fermentation broth.
- the present process can employ quantities of enzyme and yeast selected to maintain yeast at or above about 200 cells/mL, at or above about 300 cells/mL, or at about 300 to about 600 cells/mL.
- simultaneous saccharifying and fermenting can be carried out employing quantities of enzyme and yeast selected for effective fermentation without added exogenous nitrogen; without added protease; and/or without added backset. Backset can be added, if desired, to consume process water and reduce the amount of wastewater produced by the process.
- the present process maintains low viscosity during saccharifying and fermenting.
- simultaneous saccharifying and fermenting can be carried out employing quantities of enzyme and yeast selected to maintain low concentrations of soluble sugar in the fermentation broth.
- simultaneous saccharifying and fermenting can be carried out employing quantities of enzyme and yeast selected to maintain low concentrations of glucose in the fermentation broth.
- the present process can employ quantities of enzyme and yeast selected to maintain glucose at levels at or below about 2 wt-%, at or below about 1 wt-%, at or below about 0.5 wt-%, or at or below about 0.1 wt-%.
- the present process can employ quantities of enzyme and yeast selected to maintain glucose at levels at or below about 2 wt-% during saccharifying and fermenting.
- the present process can employ quantities of enzyme and yeast selected to maintain glucose at levels at or below about 2 wt-% from hours 0-10 (or from 0 to about 15% of the time) of saccharifying and fermenting.
- the present process can employ quantities of enzyme and yeast selected to maintain glucose at levels at or below about 1 wt-%, at or below about 0.5 wt-%, or at or below about 0.1 wt-% from hours 12-54 (or from about 15% to about 80% of the time) of saccharifying and fermenting.
- the present process can employ quantities of enzyme and yeast selected to maintain glucose at levels at or below about 1 wt-% from hours 54-66 (or about from 80% to about 100% of the time) of saccharifying and fermenting.
- simultaneous saccharifying and fermenting can be carried out employing quantities of enzyme and yeast selected to maintain low concentrations of maltose (DP2) in the fermentation broth.
- DP2 maltose
- the present process can employ quantities of enzyme and yeast selected to maintain maltose at levels at or below about 0.5 wt-% or at or below about 0.2 wt-%.
- simultaneous saccharifying and fermenting can be carried out employing quantities of enzyme and yeast selected to maintain low concentrations of dextrin in the fermentation broth.
- the present process can employ quantities of enzyme and yeast selected to maintain maltotriose (DP3) at levels at or below about 0.5 wt-%, at or below about 0.2 wt-%, or at or below about 0.1 wt-%.
- DP3 maltotriose
- the present process can employ quantities of enzyme and yeast selected to maintain dextrin with a degree of polymerization of 4 or more (DP4+) at levels at or below about 1 wt-% or at or below about 0.5 wt-%.
- simultaneous saccharifying and fermenting can be carried out employing quantities of enzyme and yeast selected to maintain low concentrations of fusel oils in the fermentation broth.
- the present process can employ quantities of enzyme and yeast selected to maintain fusel oils at levels at or below about 0.4 to about 0.5 wt-%.
- simultaneous saccharifying and fermenting can employ acid fungal amylase at about 0.05 to about 10 AFAU per gram of dry solids reduced plant material (e.g., DSC) and glucoamylase at about 0.5 to about 6 AGU per gram dry solids reduced plant material (e.g., DSC).
- simultaneous saccharifying and fermenting can employ acid fungal amylase at about 0.1 to about 10 AFAU per gram of dry solids reduced plant material (e.g., DSC) and glucoamylase at about 0.5 to about 6 AGU per gram dry solids reduced plant material (e.g., DSC).
- simultaneous saccharifying and fermenting can employ acid fungal amylase at about 0.3 to about 3 AFAU per gram of dry solids reduced plant material (e.g., DSC) and glucoamylase at about 1 to about 3 AGU per gram dry solids reduced plant material (e.g., DSC).
- simultaneous saccharifying and fermenting can employ acid fungal amylase at about 1 to about 2 AFAU per gram of dry solids reduced plant material (e.g., DSC) and glucoamylase at about 1 to about 1.5 AGU per gram dry solids reduced plant material (e.g., DSC).
- the saccharification and/or fermentation mixture can include additional ingredients to increase the effectiveness of the process.
- the mixture can include added nutrients (e.g., yeast micronutrients), antibiotics, salts, added enzymes, and the like.
- Nutrients can be derived from stillage or backset added to the liquid.
- Suitable salts can include zinc or magnesium salts, such as zinc sulfate, magnesium sulfate, and the like.
- Suitable added enzymes include those added to conventional processes, such as protease, phytase, cellulase, hemicellulase, exo- and endo- glucanase, xylanase, and the like.
- Ethanol can be recovered from the fermentation mixture, from the beer, by any of a variety of known processes.
- ethanol can be recovered by distillation.
- the remaining stillage includes both liquid and solid material.
- the liquid and solid can be separated by, for example, centrifugation.
- the recovered liquid, thin stillage can be employed as at least part of the liquid for forming the saccharification and fermentation mixture for subsequent batches or runs.
- the recovered solids, distiller's dried grain include unfermented grain solids and spent yeast solids.
- Thin stillage can be concentrated to a syrup, which can be added to the distiller's dried grain and the mixture then dried to form distiller's dried grain plus solubles.
- Distiller's dried grain and/or distiller's dried grain plus solubles can be sold as animal feed.
- Bum-out of Residual Starches for Subsequent Secondary Fermentation the present method can include heat treatment of the beer or stillage, e.g., between the beer well and distillation.
- the present method can include heat treatment of the beer or stillage and enzyme addition, e.g., between the beer well and distillation. This heat treatment can convert starches to dextrins and sugars for subsequent fermentation in a process known as bum-out.
- Such a treatment step can also reduce fouling of distillation trays and evaporator heat exchange surfaces.
- heat treatment staging can be performed on whole stillage or thin stillage. Following enzymatic treatment of the residual starches, in an embodiment, the resulting dextrins and sugars can be fermented within the main fermentation process as recycled backset or processed in a separate fermentation train to produce ethanol. In an embodiment, the liquefaction and saccharification on whole stillage or thin stillage produced by centrifugation can be accelerated after distillation.
- Fractionation of Solids from Fermentation Large pieces of germ and fiber can ferment the residual starch in the fermenter. After fermentation, the fractions could be removed prior to or after distillation. Removal can be effected with a surface skimmer before to distillation. In an embodiment, screening can be performed on the beer. The screened material can then be separated from the ethanol/water mix by, for example, centrifugation and rotary steam drum drying, which can remove the residual ethanol from the cake. In embodiments in which the larger fiber and germ pieces are removed prior to bulk beer distillation, a separate stripper column for the fiber/germ stream can be utilized. Alternatively, fiber and germ could be removed by screening the whole stillage after distillation. In an embodiment, all the components are blended and dried together.
- the fiber and germ can be removed from the finished product by aspiration and/or size classification.
- the fiber from the DDGS can be aspirated. Removal of fiber by aspiration after drying can increase the amount of oil and protein in the residual DDGS, for example, by 0.2 to 1.9% and 0.4 to 1.4%, respectively.
- the amount of NDF in the residual DDGS can decrease, for example, by 0.1 to 2.8%.
- fractionation can employ the larger fiber and germ pieces to increase the particle size of that part of the DDGS derived from the endosperm, as well as to improve syrup carrying capacity.
- a ring dryer disintegrator can provide some particle size reduction and homogenization.
- the beer produced by fermentation includes ethanol, other liquids, and solid material. Centrifugation and/or distillation of the beer can yield solids known as wet cake and liquids known as thin stillage.
- the wet cake can be dried to produce distiller's dried grain.
- the thin stillage can be concentrated to a syrup, which can be added to the wet cake or distiller's dried grain and the mixture then dried to form distiller's dried grain plus solubles.
- the present method can include drying the wet cake to produce distiller's dried grain.
- the present method can include drying the syrup plus distiller's dried grain to produce distiller's dried grain plus solubles.
- the distiller's dried grain can be produced from whole grain (e.g., com) or from fractionated grain (e.g., com).
- the present method can produce high protein distiller's dried grain and/or distiller's dried grain with improved physical characteristics. Such distiller's dried grains are described hereinbelow.
- Conventional ethanol production processes employed drum dryers.
- the present method and system can employ a flash or ring dryer. Flash or ring dryers have not previously been employed in processes like the present one. Configurations of flash and ring dryers are known. Briefly, a flash or ring dryer can include a vertical column through which a pre-heated air stream moves the wet cake.
- a flash or ring dryer can include one or more inlets that provide entry of heat or heated air into the dryer. This dries the wet cake. The dried wet cake is transported to the top of a column. In a ring dryer, further drying can be accomplished by moving the wet cake through one or more rings connected to the column.
- a ring dryer can include one or more inlets through which heated air enters a ring structure which propels or circulates the wet cake in or around the ring structure. The dried wet cake can then be pneumatically conveyed to down-stream separating equipment such as a cyclone or dust collector.
- the present method can include employing a flash dryer to dry (i.e., flash drying) the wet cake and to produce distiller's dried grain.
- the present method can include employing a flash dryer to dry (i.e., flash drying) the syrup plus distiller's dried grain to produce distiller's dried grain plus solubles.
- Employing a flash dryer can produce high protein distiller's dried grain and/or distiller's dried grain with improved physical characteristics. Such distiller's dried grains are described hereinbelow.
- the present method can include employing a ring dryer to dry (i.e., ring drying) the wet cake and to produce distiller's dried grain.
- the present method can include employing a ring dryer (i.e., ring drying) to dry the syrup plus distiller's dried grain to produce distiller's dried grain plus solubles.
- a ring dryer i.e., ring drying
- Employing a ring dryer can produce high protein distiller's dried grain and/or distiller's dried grain with improved physical characteristics. Such distiller's dried grains are described hereinbelow.
- the present method can include employing a fluid bed dryer to dry (i.e., fluid bed drying) the wet cake and to produce distiller's dried grain.
- the present method can include employing a fluid bed dryer to dry (i.e., fluid bed drying) the syrup plus distiller's dried grain to produce distiller's dried grain plus solubles.
- the present method can include adding syrup (backset or thin stillage) to the wet cake before, during, or after drying.
- the present method includes adding syrup (backset or thin stillage) to the wet cake during drying.
- the method can include mixing wet cake and syrup in the dryer.
- the method can include flowing or injecting syrup into the flash, ring, or fluid bed dryer.
- the present method includes adding syrup into the column or ring of the dryer in the presence of wet cake and/or distiller's dried grain.
- flash and/or ring dryers differ from rotary or drum dryers by providing decreased exposure of wet cake to high temperatures of the drying process.
- a rotary or drum dryer generally has high temperature metal that is in prolonged contact with the wet cake product. It is believed that prolonged contact of this high temperature metal with the wet cake can result in browned, burned, or denatured distiller's dried grains or distiller's dried grains plus solubles. Further, the internal air temperature can be higher in a rotary or drum dryer.
- the present method can include drying the wet cake or wet cake plus syrup for a shorter time than employed with a rotary or dram dryer, and obtaining distiller's dried grain or distiller's dried grain plus solubles that has been sufficiently dried. Accordingly, in an embodiment, the present method can include drying the wet cake or wet cake plus syrup at a lower temperature than employed with a rotary or drum dryer, and obtaining distiller's dried grain or distiller's dried grain plus solubles that has been sufficiently dried. In an embodiment, the method includes changing the drying temperature during drying. Although not limiting to the present invention, in certain embodiments, such drying systems and methods can provide one or more advantages such as decreased energy consumption in drying, decreased leakage from the drying system.
- An embodiment of this invention is the use of flash or ring dryer(s) to change the conditions inside the dryer system to increase or decrease temperature.
- An embodiment of this invention is the use of flash or ring dryer(s) to change the conditions inside the dryer system to increase or decrease the moisture.
- An embodiment of this invention is the use of flash or ring dryer(s) to change the conditions inside the dryer system to increase or decrease recycle speed.
- An embodiment of this invention is the use of flash or ring dryer(s) to change the conditions inside the dryer system to increase or decrease the feed rate into the dryer system.
- a continuous process includes moving (pumping) the saccharifying and/or fermenting mixtures through a series of vessels (e.g., tanks) to provide a sufficient duration for the process.
- vessels e.g., tanks
- a multiple stage fermentation system can be employed for a continuous process with 48-96 hours residence time.
- reduced plant material e.g., fractionated plant material
- Partially incubated and fermented mixture can then be drawn out of the bottom of the first vessel and fed in to the top of a second vessel, and so on.
- the present method is more suitable than conventional methods for running as a continuous process. It is believed that the present process provides reduced opportunity for growth of contaminating organisms in a continuous process.
- the majority of dry grind ethanol facilities employ batch fermentation technology. This is in part due to the difficulty of preventing losses due to contamination in these conventional processes.
- the conventional belief is that a separate saccharification stage prior to fermentation is necessary to pre-saccharify the mash for fermentation. Such pre-saccharification insures that there is adequate fermentable glucose for the continuous fermentation process.
- the present method achieves efficient production of high concentrations of ethanol without a liquefaction or saccharification stage prior to fermentation.
- the present process can ferment a portion of a reduced plant material, such as com.
- the process can ferment at least one of endosperm, fiber, or germ.
- the present process can increase ethanol production from such a portion of com.
- the present process can saccharify and ferment endosperm.
- Endosperm fermentation is lower in free amino nitrogen (FAN) towards the beginning of fermentation due to the removal of germ, which contains FAN.
- FAN free amino nitrogen
- the present process can, for example, preserve the FAN quality of the endosperm compared to conventional high temperature liquefaction.
- An embodiment of the present invention includes the use of endosperm FAN, which can increase flexibility and efficiency of fermentation.
- the present process can employ endogenous enzyme activity in the grain.
- dramatic increase in FAN in whole com and defibered com fermentations are reached compared to the initial mash slurry.
- Conventional grain dry milling operations separate germ (containing oil) and bran or pericarp (fiber fraction) from the endosperm (starch and protein) portion of the grain using a series of steps and procedures. These steps and procedures include: grain cleaning, tempering, degerming, particle size reduction, roller milling, aspirating, and sifting. This process differs from the traditional wet milling of grains (commonly com) which are more expensive and water intensive, but capable of achieving cleaner separations of the components of the grain.
- Dry milling processes offer a version of separating components using lower capital costs for facilities. Also, these processes require less water for operation. The tempering process in dry milling requires less water than required in wet milling. The competitiveness of dry grain fractionation processes is enhanced when the process of the present invention is utilized for ethanol conversion of these fractions.
- dry milling processes produce various grades of each fraction (germ, bran, and endosperm).
- the present method provides bran and endosperm fractions that can be more readily fermented. Depending on the desired purity of each fraction, the fractions can either be pooled to create composites of each stream, or the fractions can be processed individually.
- Yeast uses FAN in the present process.
- FAN levels fall throughout fermentation as yeast cells assimilate and metabolize available FAN during the course of fermentation.
- FAN utilization kinetics in the raw starch process is more rapid.
- Some of the increase of FAN is due to yeast cell death resulting from the accelerated fermentation.
- High Alcohol Beer The present invention also relates to a high alcohol beer. In an embodiment, the process of the present invention produces beer containing greater than 18 vol-% ethanol.
- the present process can produce such a high alcohol beer in about 40 to about 96 hours or about 45 to about 96 hours.
- the beer includes 18 vol-% to about 23 vol-% ethanol.
- the present method can produce alcohol contents in the fermenter of 18 to 23 % by volume in about 45 to 96 hours.
- the present method can produce alcohol content in the fermenter of 18 to 23 % by volume in about 45 to 96 hours.
- the majority of the alcohol 80% or more of the final concentration
- an additional 2 to 5 vol-% alcohol can be produced in the final 12-48 hours. Concentrations of ethanol up to 23 vol-% can be achieved with fermentation time up to 96 hours.
- the present beer can include this high level of ethanol even when it includes high levels of residual starch.
- the present beer can include ethanol at 18 to 23 vol-% when it contains 0 to 30% residual starch.
- the present beer can contain residual starches as low as 0% to as high as 20% residual starch.
- high levels of residual starch indicate inefficient fermentation, which yields only low levels of ethanol.
- it is believed that the present method results in fewer Maillard type reaction products and more efficient yeast fermentation (e.g., reduced levels of secondary metabolites).
- the present method can produce more alcohol even with higher levels of residual starch.
- the present beer includes fewer residual byproducts than conventional beers, even though residual starch can be higher.
- residual glucose, maltose, and higher dextrins can be as much as 0.8 wt-% lower than in conventional beers produced under similar fermentation conditions.
- residual glycerol can be as much as 0.7 wt-% less. Lactic acid and fusel oils can also be significantly reduced.
- the present beer can include less than or equal to about 0.2 wt-% glucose, about 0.4 wt-%, about 0.1 wt-% DP3, undetectable DP4+, 0.7 wt-% glycerol, about 0.01 wt-% lactic acid, and/or about 0.4 wt-% fusel oils.
- the present invention also relates to a distiller's dried grain product.
- the distiller's dried grain can also include elevated levels of one or more of protein, fat, fiber (e.g., neutral detergent fiber (NDF)), and starch.
- NDF neutral detergent fiber
- the present distiller's dried grain can include 34 or more wt-% protein, about 25 to about 60 wt-% protein, about 25 to about 50 wt-% protein, or about 30 to about 45 wt-% protein. In certain circumstances the amount of protein is about 1 to about 2 wt-% more protein than produced by the conventional process.
- the distiller's dried grain can include 15 or more wt-% fat, about 13 to about 17 wt-% fat, or about 1 to about 6 wt-% more fat than produced by the conventional process.
- the distiller's dried grain can include 31 or more wt-% fiber, about 23 to about 37 wt-% fiber, or about 3 to about 13 wt-% more fiber than produced by the conventional process.
- the distiller's dried grain can include 12 or more wt-% starch, about 1 to about 23 wt-% starch, or about 1 to about 18 wt-% more starch than produced by the conventional process.
- the present distiller's dried grain includes elevated levels of B vitamins, vitamin C, vitamin E, folic acid, and/or vitamin A, compared to conventional distiller's dried grain products.
- the present distiller's dried grain has a richer gold color compared to conventional distiller's dried grain products.
- the present invention also relates to a distiller's dried grain with one or more improved physical characteristics, such as decreased caking or compaction or increased ability to flow.
- the present process can produce such an improved distiller's dried grain.
- the present process can produce fermentation solids including higher molecular weight forms of carbohydrates.
- Such fermentation solids can, it is believed, exhibit a higher glass transition temperature (i.e. higher T g values) compared to solids from the conventional process.
- T g values glass transition temperature
- residual starches can have a high T g value.
- the present process can manufacture DDG or DDGS with target T g values.
- adding an alkaline syrup blend e.g., syrup plus added lime or other alkaline material
- the fermentation solids e.g., distiller's dried grains
- DDGS distiller's dried grain with solubles
- organic acids such as lactic, acetic, and succinic acids which are produced in fermentation have a lower T g value than their corresponding calcium salts.
- DDG or DDGS of or produced by the method of the present invention flows more readily than DDG or DDGS produced by the conventional process.
- process of the present invention can need not destroy protein in the fermented plant material (e.g., fractionated plant material).
- Com contains prolamins, such as zein.
- Grain sorghum for example, contains a class of zein-like proteins known as kafirins, which resemble zein in amino acid composition.
- the thermal degradation that occurs during liquefaction, distillation, and high temperature drying produces DDG and DDGS including significant amounts of degraded protein.
- the process of the present invention can provides improved levels of the prolamin fraction of cereal grains. It is believed that extended exposure to high alcohol concentrations that can be achieved by the present process can condition the proteins in the plant material (e.g., fractionated plant material). This can solubilize some of the proteins. For example, it is believed that in distillation the ethanol concentration reaches levels that can solubilize prolamins (e.g., zein) in the beer.
- prolamins e.g., zein
- prolamins such as zein
- DDG and DDGS can be recovered in concentrated form in DDG and DDGS.
- the resulting high protein content of DDG and DDGS can be advantageous for various end uses of DDG and DDGS, for example in further processing or compounding.
- efficient fermentation of the present process removes from the DDG or DDGS non zein components such as starch.
- Fractionating the plant material, e.g., com can also increase levels of proteins, such as zein, in the DDG or DDGS.
- removing the bran and germ fractions prior to fermentation can concentrate zein in the substrate.
- Zein in com is isolated in the endosperm. Fermentation of zein enriched endosperm results in concentration of the zein in the residuals from fermentation.
- the present method can operate on fractionated plant material
- the present method operated on fractionated plant material can produce a DDG enriched in prolamin, such as zein.
- the process of the present invention can provide DDG and DDGS with different, predetermined T g values.
- the process of the present invention can ferment fractions containing high, medium, or low levels of zein, thus varying the glass transition temperature of the resulting DDG or DDGS.
- the resulting co-product T g can be directly proportional to the prolamin protein (such as zein) content.
- the process of the current invention is desirable for the fermentation of high protein com.
- the wet cake fraction produced by the present method which can be dried separately to produce DDG, can be higher in prolamin protein (such as zein) than conventional DDG.
- the present process allows syrup and wet cake blend ratios to be varied. This results in DDG/DDGS with varying ratios of prolamin protein (such as zein) and residual starch. As the residual starch in the wet cake reduces the protein in the wet cake increases. This indicates an inverse relationship. A similar response occurs in the syrup fraction.
- starch can segregate into the liquid fraction.
- the amount of starch in the DDGS can be varied by blending syrup at rates ranging from 0 lbs. dry weight of syrup solids to 1.2 lbs. of syrup solids per lb. of wet cake solids before, and various times during drying to create the final DDGS product.
- the disproportionate segregation of residual starches into the backset or thin stillage fraction can provide both the aforementioned bum-out and secondary fermentation to be performed on these fractions. Since the thin stillage is evaporated to produce syrup, the centrifuge mass balance also enables DDGS production at various T s values depending on the desired properties and their dependence on T g .
- Emissions benefits result in the reduction in byproducts created in the ethanol manufacturing process. There is a marked reduction in extraction of fats and oils in the mash from the germ fraction of cereal grains. There is a reduction of byproducts from Maillard reactions typically formed during cooking and liquefaction. And there is a reduction in fermentation byproducts. These observations result in reduced emissions during the recovery of co- products.
- the concentration and emission rates of volatile organic compounds (NOC), carbon monoxide (CO), nitric oxide compounds ( ⁇ Ox), sulfur oxides (SO2), and other emissions are considerably lower. See Table 1. Note that other manufacturers have attempted to lower emissions by manufacturing wet cake instead of drying to DDG or DDGS.
- the present invention also relates to volatile organic compounds (VOC), such as those produced by drying products of a fermentation process.
- VOC volatile organic compounds
- the present method includes producing ethanol, distiller's dried grain, and additional useful fermentation products with production of lower levels of VOC compared to conventional processes.
- drying distillation products e.g., spent grain
- Conventional fermentation processes using com produces about 2.1 pounds of NOC's from drying distillation products from each ton of com processed.
- the actual stack emissions can be less due to pollution control equipment.
- the present method results in at least 30% reduction in VOC production to about 1.47 or less pounds per ton of com processed.
- VOC produced by fermentation processes include ethanol, acetic acid, formaldehyde, methanol, acetaldehyde, acrolein, furfural, lactic acid, formic acid, and glycerol.
- the present invention also relates to carbon monoxide (CO), such as those produced by drying products of a fermentation process.
- CO carbon monoxide
- the present method includes producing ethanol, distiller's dried grain, and additional useful fermentation products with production of lower levels of CO compared to conventional processes. For example, in the present method, drying distillation products (e.g., spent grain) produces reduced levels of CO.
- the invention relates to a system that produces ethanol.
- the present system can include a saccharification apparatus 1, a fermentation apparatus 2, a distillation apparatus 3, and a dryer apparatus 4.
- the saccharification apparatus 1 can be any of a variety of apparatus suitable for containing or conducting saccharification.
- the saccharification apparatus 1 can be, for example, a vessel in which reduced plant material can be converted to a sugar, which can be fermented by a microorganism such as yeast.
- the saccharification apparatus 1 can be configured to maintain a saccharification mixture under conditions suitable for saccharification.
- the saccharification apparatus 1 can be configured to provide for the conversion of reduced plant material with the addition of enzymes.
- the saccharification apparatus 1 is configured for mixing reduced plant material with a liquid and adding a saccharifying enzyme composition to the liquid.
- the saccharification apparatus 1 is configured for saccharification at a variety of pHs and temperatures, but preferably at a pH of 6.0 or less, and at a temperature of about 25 to about 40 °C.
- the fermentation apparatus 2 can be any of a variety of apparatus suitable for containing or conducting fermentation.
- the saccharification apparatus 1 can be, for example, a vessel in which sugar from reduced plant material can be fermented to ethanol.
- the fermentation apparatus 2 can be configured to maintain a fermentation mixture under conditions suitable for fermentation.
- the fermentation apparatus 2 can be configured for fermenting through use of a microorganism, such as yeast.
- the fermentation apparatus 2 can be configured to ferment a starch composition that has not been cooked, specifically the saccharification mixture.
- the apparatus can employ any variety of yeasts that yields a commercially significant quantity of ethanol in a suitable time.
- Yeast can be added to the apparatus by any of a variety of methods known for adding yeast to a system that conducts fermentation.
- the fermentation apparatus 2 can be configured for fermentation for about 25 to 150 hours at a temperature of about 25 to about 40 degrees C.
- the saccharification apparatus 1 and the fermentation apparatus 2 can be a single, integrated apparatus. In an embodiment, this apparatus is configured to provide higher temperatures early on during simultaneous conversion of reduced plant material to sugars and fermentation of those sugars.
- this apparatus is configured to provide lower temperatures later during the simultaneous saccharification and fermentation.
- the apparatus also may utilize the reagents and conditions described above for saccharification and fermentation, including enzymes and yeast.
- the distillation apparatus 3 can be any of a variety of apparatus suitable for distilling products of fermentation.
- the distillation apparatus 3 can be, for example, configured to recover ethanol from the fermentation mixture ("beer").
- the fermentation mixture is treated with heat prior to entering the distillation apparatus 3.
- fractions of large pieces of germ and fiber are removed with a surface skimmer or screen prior to or after entering the distillation apparatus 3.
- the dryer apparatus 4 can be any of a variety of apparatus suitable for drying solids remaining after distillation (and optional centrifugation, for example, in a centrifuge system).
- the dryer apparatus 4 is configured to dry recovered solids, which can result in production of distiller's dried grain. After the distillation system separates the ethanol from the beer, recovered solids remain. These recovered solids can then be dried in the dryer apparatus 4. This produces distiller's dried grain and/or distiller's dried grain plus solubles.
- the dryer apparatus 4 can be or include a ring dryer. In an embodiment, the dryer apparatus 4 can be or include a flash dryer. In an embodiment, the dryer apparatus 4 can be or include a fluid bed dryer.
- Example 1 - The Present Process Provides Improved Efficiency With Substrates Derived From Grain Dry Milling Operations (Endosperm, Fiber, & Germ)
- the present invention provides an improved method for fermenting substrates derived from grain milling (dry fractionation) processes.
- the present process is useful for endosperm fermentation since FAN levels in the mash are reduced to the removal of germ.
- the present process contributes to the endogenous enzymes activity in the grain. Dramatic increase in FAN in whole com and defibered com fermentations are reached compared to the initial mash slurry.
- the present process also enables the endogenous enzyme activity in the grain to contribute to the generation of soluble sugars and amino nitrogen in the mash. These beneficial activities are lost during the conventional liquefaction stage.
- the kinetics of FAN utilization is illustrated in Figure 2B for fermentation of various dry milled grain fractions. It is interesting to note that FAN kinetics in the conventional process all follow a similar utilization pathway for each com fraction. During the first half of fermentation, FAN is consumed in the course of yeast growth. Later, FAN levels are observed to increase, presumably due the liberation of cellular FAN corresponding to yeast cell death and lysis. Initial FAN utilization in the raw starch process is observed to be much more rapid. Also note the dramatic increase in FAN at the end of raw starch fermentations.
- FIG. 2C illustrates the impact of FAN on com fraction fermentations ran in the absence of backset, comparing and contrasting the sensitivity of the two processes to additional FAN addition. It is apparent that the process of the present invention significantly improves the potential substrate quality from a dry milling fractionation facility for fermentation, reducing the importance of additional FAN.
- the present process is superior to the conventional liquefaction process, since the conventional liquefaction process is more sensitive to disruptive impact of substrate quality as measured by FAN levels.
- Example 2 The Present Method Produced High Protein DDG From Fractionated Plant Product The present invention demonstrated that fractionation of com prior to fermentation provides high levels of protein in the resulting DDG.
- compositions were fractionated prior to fermentation through use of a Satake fractionation system. After fractionation, the com was fermented according to the present invention employing for saccharification glucoamylase and acid fungal amylase without cooking. The fermentation was conducted at 90 °F and at a pH of 5. After the com solids were fermented, the ethanol was distilled out. The remaining solids were then dried, and samples of fiber, germ, and starch were taken. All fractionation samples were ground for twenty seconds on a Knifetec. These samples were then analyzed for starch, protein, fat, and neutral detergent fiber content. The percent ethanol yield was also calculated for each sample. See also the Materials and Methods sections for the other examples for additional information about how these experiments were conducted.
- Table 2 shows results for ethanol and DDG produced from two representative samples of each of fiber, starch, and germ samples. Fermentations B and C, the representative starch samples, resulted in the highest yield of ethanol and produced DDG with the largest percentage of protein (Table 2). The two germ samples generated the lowest yield of ethanol and the highest percentage of fat (Table 2). The fiber samples produced the lowest amount of protein (Table 2). In general, this table illustrates that fractionation increased the rate of protein retention throughout the fermentation and distillation process (Table 2).
- Example 3 The Present Process Provided Improved Corn Fiber Fermentation
- the present invention provides an improved method for fermenting com fiber substrates derived from grain milling (dry fractionation) processes.
- the present process was useful for gentler removal of starch from com fiber fractions via fermentation.
- com fiber fractions contain recalcitrant starch deposits.
- the present method provided improved access to the starch present in the com fiber.
- the present process provided effective com fiber fermentation (Table 3).
- Table 3 indicate the positive impact of fiber fermentation as measured by ethanol yield using the present method. Varying the temperature displays the effect on ethanol recovery, with efficient ethanol recovery produced at lower temperatures.
- the present method effectively fermented a com fiber fraction that in a conventional process typically stalls fermentation.
- Example 4 The Present Process Provided Improved Ethanol Kinetics In Endosperm Fermentation Via Additional Germ or Germ Meal
- the present invention provides an improved method for fermenting fractionated grain, such as fractionated com derived from a grain milling (dry fractionation) process.
- Liquizyme SC AA (0.30 ml of a 25X) was used. Fermentation standard ingredients at plant equivalent dose (lab dose) to include 660 L Spirizyme Plus glucoamylase (.25 ml of a 10X), 33 L protease (0.13 ml of a 100X), 4.4 lbs Lactrol (0.16 ml of a 2,000X), and no urea liquor. Fermentation temperature staging conditions included 90°F from 0-24 hours, 84°F from 24-48 hours, and 82°F from 48-65 hours.
- Yeast propagator standard ingredients at laboratory dose to include 230 mL deionized water, 100 mL backset, 70 grams maltodextrin MO40, 0.44 mL of a 5x, 1.76 mL of a 100X, 1.07 grams, 1.07 grams, 1.70 mL of a 1000X, 0.13 grams zinc sulfate, 0.48 grams Fali Yeast for an eight (8) hour propagation, propagation temperature of ninety degrees (90 °F), with a 2.88 mL transfer of yeast propagator to each fermenter for inoculation.
- Plant scale dosages refer to 550,000 gallon fermenters with 80 mL lab fermenters used. The grams of flour used and the makeup water added was adjusted for each fermenter to keep the starch content consistent.
- the pH of all fermenters was adjusted to 6.0 with sulfuric acid. All endosperm flour used was collected from BEI already ground, and all germ flour was ground in the KnifeTech mill (3x10 sec). The whole com used as control was ground through a Lab 1.0 mm Screen. The pH of all drop samples was adjusted to less than 3.50 with sulfuric acid to deactivate residual enzyme activity prior to drying samples for proximate analysis.
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Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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EP10181812.8A EP2281898B1 (fr) | 2004-03-10 | 2005-03-10 | Distiller's grains sechées avec haute percentage en proteines et méthode de production |
Applications Claiming Priority (4)
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US55210804P | 2004-03-10 | 2004-03-10 | |
US61491604P | 2004-09-30 | 2004-09-30 | |
US61515504P | 2004-10-01 | 2004-10-01 | |
PCT/US2005/008156 WO2005087938A2 (fr) | 2004-03-10 | 2005-03-10 | Procedes et systemes servant a produire de l'ethanol au moyen d'amidon brut et d'un fractionnement |
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EP10181812.8A Division EP2281898B1 (fr) | 2004-03-10 | 2005-03-10 | Distiller's grains sechées avec haute percentage en proteines et méthode de production |
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EP1723248A2 true EP1723248A2 (fr) | 2006-11-22 |
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EP10181812.8A Active EP2281898B1 (fr) | 2004-03-10 | 2005-03-10 | Distiller's grains sechées avec haute percentage en proteines et méthode de production |
EP05725366A Withdrawn EP1723248A2 (fr) | 2004-03-10 | 2005-03-10 | Procedes et systemes servant a produire de l'ethanol au moyen d'amidon brut et d'un fractionnement |
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EP10181812.8A Active EP2281898B1 (fr) | 2004-03-10 | 2005-03-10 | Distiller's grains sechées avec haute percentage en proteines et méthode de production |
Country Status (5)
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EP (2) | EP2281898B1 (fr) |
AU (1) | AU2005222426A1 (fr) |
BR (1) | BRPI0508606A (fr) |
CA (1) | CA2559015C (fr) |
WO (2) | WO2005087937A2 (fr) |
Families Citing this family (19)
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JP5087407B2 (ja) | 2004-12-30 | 2012-12-05 | ジェネンコー・インターナショナル・インク | 酸性真菌プロテアーゼ |
US20080003653A1 (en) * | 2006-06-29 | 2008-01-03 | Wenzel J Douglas | Supplementation of ethanol fermentations and processes including supplemental components |
DE102006033791A1 (de) * | 2006-07-19 | 2008-01-24 | Prokon Nord Energiesysteme Gmbh | Verbesserte Verfahren zur Herstellung von Ethanol, Gluten und und Kleie aus Getreide |
US9499635B2 (en) | 2006-10-13 | 2016-11-22 | Sweetwater Energy, Inc. | Integrated wood processing and sugar production |
DE102007028012A1 (de) * | 2007-04-10 | 2008-10-16 | Fritzmeier, Ursula | Verfahren zur Herstellung von Ethanol und Ethanolreaktor |
EP2329033A4 (fr) * | 2008-08-27 | 2012-05-16 | Edeniq Inc | Matériel et méthodes de conversion de biomasse en biocarburant |
WO2010068637A1 (fr) | 2008-12-09 | 2010-06-17 | Jerry Wayne Horton | Biomasse d'ensilage et appareil à phases multiples pour une hydrolyse de biomasse ensilée |
US9175315B2 (en) | 2010-06-18 | 2015-11-03 | Butamax Advanced Biofuels Llc | Production of alcohol esters and in situ product removal during alcohol fermentation |
HUE032506T2 (en) * | 2011-06-17 | 2017-09-28 | Butamax Advanced Biofuels Llc | By-products from biofuel processes and processes |
US8765430B2 (en) | 2012-02-10 | 2014-07-01 | Sweetwater Energy, Inc. | Enhancing fermentation of starch- and sugar-based feedstocks |
US8563277B1 (en) | 2012-04-13 | 2013-10-22 | Sweetwater Energy, Inc. | Methods and systems for saccharification of biomass |
US8722911B2 (en) | 2012-06-20 | 2014-05-13 | Valicor, Inc. | Process and method for improving the water reuse, energy efficiency, fermentation, and products of an ethanol fermentation plant |
US9334516B2 (en) | 2013-03-14 | 2016-05-10 | Abengoa Bioenergy New Technologies, Inc. | Method for adding enzymes to obtain high ethanol yield from cereal mash |
WO2014143753A1 (fr) | 2013-03-15 | 2014-09-18 | Sweetwater Energy, Inc. | Purification de carbone de courants de sucre concentré issus de biomasse prétraitée |
CA2969840A1 (fr) | 2014-12-09 | 2016-06-16 | Sweetwater Energy, Inc. | Pretraitement rapide |
BR112017017831A2 (pt) * | 2015-03-04 | 2018-04-10 | Danisco Us Inc | processo de produção de bioálcool |
BR112019017106A2 (pt) | 2017-02-16 | 2020-04-28 | Sweetwater Energy Inc | formação de zona de alta pressão para pré-tratamento |
AU2020287166A1 (en) * | 2019-06-05 | 2022-01-06 | Danisco Us Inc | Methods for improving the amino acid content of animal feed products |
EP4077490A1 (fr) | 2019-12-22 | 2022-10-26 | Sweetwater Energy, Inc. | Procédés de fabrication de lignine et de produits de lignine spécialisés à partir de biomasse |
Citations (2)
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US20030180415A1 (en) * | 2002-03-20 | 2003-09-25 | Stiefel Michael J. | High protein concentrate from cereal grain and methods of use thereof |
US20030232109A1 (en) * | 2001-12-04 | 2003-12-18 | Dawley Larry J. | High protein corn product production and use |
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US4279747A (en) * | 1980-01-17 | 1981-07-21 | Fabcon Incorporated | Rapid continuous clarifier |
US5231017A (en) * | 1991-05-17 | 1993-07-27 | Solvay Enzymes, Inc. | Process for producing ethanol |
KR0152482B1 (ko) * | 1995-09-29 | 1998-10-01 | 최차용 | 발효에 의한 균주 대사산물의 연속적 제조 방법 |
US6726133B2 (en) | 1997-07-18 | 2004-04-27 | Pulsewave Llc | Process for micronizing materials |
US6648500B2 (en) | 1999-04-13 | 2003-11-18 | International Process Equipment And Technology, Inc. | Rotary pulsation device |
US7141260B2 (en) | 2002-08-29 | 2006-11-28 | Pioneer Hi-Bred International, Inc. | Apparatus and method for removal of seed pericarp |
CA2517920C (fr) * | 2003-03-10 | 2012-12-18 | Broin And Associates, Inc. | Procede de production d'ethanol a partir d'amidon brut |
-
2005
- 2005-03-10 AU AU2005222426A patent/AU2005222426A1/en not_active Abandoned
- 2005-03-10 WO PCT/US2005/008155 patent/WO2005087937A2/fr active Application Filing
- 2005-03-10 EP EP10181812.8A patent/EP2281898B1/fr active Active
- 2005-03-10 EP EP05725366A patent/EP1723248A2/fr not_active Withdrawn
- 2005-03-10 BR BRPI0508606-0A patent/BRPI0508606A/pt not_active Application Discontinuation
- 2005-03-10 WO PCT/US2005/008156 patent/WO2005087938A2/fr active Application Filing
- 2005-03-10 CA CA2559015A patent/CA2559015C/fr active Active
Patent Citations (2)
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US20030232109A1 (en) * | 2001-12-04 | 2003-12-18 | Dawley Larry J. | High protein corn product production and use |
US20030180415A1 (en) * | 2002-03-20 | 2003-09-25 | Stiefel Michael J. | High protein concentrate from cereal grain and methods of use thereof |
Also Published As
Publication number | Publication date |
---|---|
WO2005087937A3 (fr) | 2006-02-09 |
EP2281898B1 (fr) | 2016-08-10 |
AU2005222426A1 (en) | 2005-09-22 |
WO2005087937A2 (fr) | 2005-09-22 |
CA2559015C (fr) | 2014-07-29 |
EP2281898A1 (fr) | 2011-02-09 |
WO2005087938A2 (fr) | 2005-09-22 |
BRPI0508606A (pt) | 2007-07-31 |
CA2559015A1 (fr) | 2005-09-22 |
WO2005087938A3 (fr) | 2006-05-26 |
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