EP1644765A1 - Source lumineuse pourvue d'un element optique microstructure et microscope a source lumineuse - Google Patents

Source lumineuse pourvue d'un element optique microstructure et microscope a source lumineuse

Info

Publication number
EP1644765A1
EP1644765A1 EP04766239A EP04766239A EP1644765A1 EP 1644765 A1 EP1644765 A1 EP 1644765A1 EP 04766239 A EP04766239 A EP 04766239A EP 04766239 A EP04766239 A EP 04766239A EP 1644765 A1 EP1644765 A1 EP 1644765A1
Authority
EP
European Patent Office
Prior art keywords
light
light source
microscope
optics
optical element
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP04766239A
Other languages
German (de)
English (en)
Inventor
Kyra MÖLLMANN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leica Microsystems CMS GmbH
Original Assignee
Leica Microsystems Heidelberg GmbH
Leica Microsystems CMS GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leica Microsystems Heidelberg GmbH, Leica Microsystems CMS GmbH filed Critical Leica Microsystems Heidelberg GmbH
Publication of EP1644765A1 publication Critical patent/EP1644765A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/24—Coupling light guides
    • G02B6/42—Coupling light guides with opto-electronic elements
    • G02B6/4201—Packages, e.g. shape, construction, internal or external details
    • G02B6/4204—Packages, e.g. shape, construction, internal or external details the coupling comprising intermediate optical elements, e.g. lenses, holograms
    • G02B6/421—Packages, e.g. shape, construction, internal or external details the coupling comprising intermediate optical elements, e.g. lenses, holograms the intermediate optical component consisting of a short length of fibre, e.g. fibre stub
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/02—Optical fibres with cladding with or without a coating
    • G02B6/02295—Microstructured optical fibre

Definitions

  • the invention relates to a light source with a microstructured optical element that spectrally broadened the light from a primary source, and with an optic that forms the spectrally broadened light into an illuminating light beam.
  • the invention relates to a microscope that includes a light source with a microstructured optical element that spectrally broadens the light from a primary source and with optics that form the spectrally broadened light into an illuminating light beam.
  • the patent US 6,097,870 discloses an arrangement for generating a broadband spectrum in the visible and infrared spectral range.
  • the arrangement is based on a microstructured fiber into which the light from a pump laser is coupled.
  • the pump light is broadened in the microstructured fiber by nonlinear effects.
  • So-called photonic band gap material or "photon crystal fibers”, “holey fibers” or “microstructured fibers” are also used as microstructured fibers. Refinements are also known as so-called “hollow fibers”.
  • Another arrangement for generating a broadband spectrum is shown in US Pat Publication by Birks et al .: “Supercontinuum generation in tapered fibers", Opt.Lett. Vol. 25, pJ415 (2000).
  • a conventional optical fiber with a fiber core is used which has a taper at least along a section
  • Optical fibers of this type are known as so-called “tapered fibers”.
  • Biological tissue or, for example, prepared with fluorescent dyes are used as samples
  • the illuminating light reflected from the sample is often detected, and solid-state lasers and dye lasers, as well as fiber lasers and optical parametric oscillators (OPO), which are preceded by a pump laser, are also frequently used.
  • OPO optical parametric oscillators
  • the object is achieved by a light source which is characterized in that the optics compensate for the different divergences of the spectral components of the spectrally broadened light.
  • the light source according to the invention advantageously generates an illuminating light beam, the light of which can be collimated simultaneously for all of its spectral components. This has an extremely favorable effect, particularly in scanning microscopy, since it is of crucial importance that all spectral components of the illuminating light beam find their focus in the sample plane to be observed.
  • the optics have a different focal length for light-different wavelengths. It is sufficient for many applications that the optics take into account and correct a linear dependence of the divergence on the wavelength in the first order. For highly specialized applications, the optics are preferably adapted exactly to the spectral properties of the microstructured fiber.
  • the optics focus the shorter-wave spectral components of the spectrally broadened light more than the longer-wave spectral components of the spectrally broadened light.
  • the microstructured optical element preferably contains photonic band-gab material and is additionally preferably configured as an optical fiber (photonic crystal fiber PCS; Holey fiber, etc.).
  • the microstructured optical element designed as an optical fiber has a tapered fiber.
  • an aperture is provided which blocks out the marginal rays of the spectrally broadened light.
  • This aperture takes into account the effect that, with increasing wavelength, more light enters the cladding of the microstructured optical fiber, which is visible at the output of the microstructured optical fiber, even though the light output portion of this light is significantly less than that of the spectrally broadened, which emerges directly from the core light.
  • the optics preferably have the spectrally broadened light Illumination light beam forms a suitable aperture.
  • the optics are part of a microscope, in particular a scanning microscope or a confocal scanning microscope. It is particularly advantageous if the optics are designed as lenses.
  • the light source according to the invention can also be used, for example, in a flow cytometer or an endoscope or a chromatograph or a lithography device.
  • the microstructured optical element is constructed from a multiplicity of microoptical structural elements which have at least two different optical densities.
  • the optical element includes a first region and a second region, the first region having a homogeneous structure and a microscopic structure formed from micro-optical structure elements in the second region. It is also advantageous if the first area encloses the second area.
  • the micro-optical structural elements are preferably cannulas, webs, honeycombs, tubes or cavities.
  • the microstructured optical element consists of glass or plastic material and cavities arranged side by side.
  • the variant in which the microstructured optical element consists of photonic band gap material and is designed as an optical fiber is particularly preferred.
  • An optical diode is preferably provided between the laser and the optical fiber, which suppresses back reflections of the light beam which originate from the ends of the optical fiber.
  • a very particularly preferred and easy-to-implement embodiment variant includes, as a microstructured optical element, a conventional optical fiber with a fiber core diameter of approximately 9 ⁇ m, which has a taper at least along a section.
  • Optical fibers of this type are known as so-called “tapered fibers”.
  • the optical fiber is a total of 1 m long and has a taper over a length of 30 mm to 90 mm.
  • the diameter of the entire fiber in the region of the taper is approximately 2 ⁇ m.
  • Fig. 2 shows the beam path of spectrally broadened light without
  • FIG. 5 shows a further light source according to the invention and FIG. 6 shows a confocal scanning microscope according to the invention.
  • the microstructured optical fiber 5 widens the light 7 of a primary light source 9, which is designed as a pulse laser 11.
  • a primary light source 9 which is designed as a pulse laser 11.
  • an optical system 13 which forms the spectrally broadened light into an illuminating light beam 15, the optical system 13 compensating for the different divergences of the spectral components of the spectrally broadened light which emerges from the microstructured optical fiber 5.
  • a focusing optics 17 which focuses the light of the primary light source onto the entry end of the microstructured optical fiber 5.
  • the components are located in a housing 19. 2 shows an example of the course of a first light bundle 21 from the red spectral range and a second light bundle 23 from the blue spectral range of the spectrally broadened light as it emerges from a microstructured optical fiber 5.
  • the microstructured optical fiber 5 has a cladding 25 and a fiber core 27 , It can also be seen schematically that the first light bundle 21 partly exits from the red spectral range from the cladding 25, while the second light bundle 23 essentially emerges from the fiber core from the blue spectral range.
  • the optics preferably also compensate for this fact of afocality.
  • FIG. 3 shows an arrangement according to the prior art, in which the optics 13 are designed as achromatic optics 29 for shaping an illuminating light beam 15. While the second light bundle 23, which contains light from the blue spectral range, is collimated by the achromatic optics 29, the light from the first light bundle 21, which contains light from the red spectral range, is disadvantageously focused. The two light beams 21, 23 consequently do not simultaneously form a parallel beam and therefore only an illuminating light beam of reduced optical beam quality.
  • FIG. 3 shows an arrangement according to the prior art, in which the optics 13 are designed as achromatic optics 29 for shaping an illuminating light beam 15. While the second light bundle 23, which contains light from the blue spectral range, is collimated by the achromatic optics 29, the light from the first light bundle 21, which contains light from the red spectral range, is disadvantageously focused. The two light beams 21, 23 consequently do not simultaneously form a parallel beam and therefore only an illuminating light beam of reduced optical beam quality.
  • FIG. 4 illustrates the course of the first light bundle 21, which contains light from a red spectral component, and of the second light bundle 23, which contains light from a blue spectral component, when using an optical system 13, which performs optical system 31 that is matched to the spectral properties of the microstructured optical optical fiber 5 is.
  • both the first light bundle 21, which contains light from the red spectral range of the spectrally dispersed light, and the light of the second light bundle 23, which contains light from the blue spectral range of the spectrally broadened light run collimated parallel to one another and form one Illuminating light beam with perfect optical beam properties.
  • FIG. 5 shows an embodiment variant of the light source in which a variable diaphragm 33 is provided in order to block out the light emerging from the cladding 25 of the microstructured optical element 3.
  • FIG. 6 shows a scanning microscope according to the invention, which is designed as a confocal scanning microscope.
  • the illuminating light beam 15 emanating from a light source 1 with a microstructured optical element not shown in this figure is focused by the lens 35 onto the illuminating pinhole 37 and then arrives at the main beam splitter 39, which directs the illuminating light beam 15 to the beam deflecting device 41, which has a gimbal-mounted scanning mirror 43 includes, directs.
  • the beam deflection device 41 guides the illuminating light beam 15 through the scan lens 45 and the tube lens 47 as well as through the objective 49 over or through the sample 51.
  • the detection light 53 emanating from the sample arrives in the reverse light path , namely through the lens 49, the tube lens 47 and through the scan lens 45 back to the beam deflection device 41 and to the main beam splitter 39, passes this and after passing through the detection pinhole 55 reaches the detector 57, which is designed as a multiband detector 59.
  • the detection light is detected in various spectral detection channels and electrical signals proportional to the power are generated, which are forwarded to a processing system (not shown) for displaying an image of the sample 51.

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microscoopes, Condenser (AREA)
  • Optical Couplings Of Light Guides (AREA)
  • Lenses (AREA)

Abstract

L'invention concerne une source lumineuse pourvue d'un élément optique microstructuré qui disperse spectralement la lumière d'une source primaire. Cette source lumineuse présente une optique qui forme un faisceau lumineux d'éclairage à partir de la lumière dispersée spectralement. Cette optique compense les différentes divergences des composantes spectrales de la lumière dispersée spectralement. Cette invention concerne également un microscope.
EP04766239A 2003-07-15 2004-07-15 Source lumineuse pourvue d'un element optique microstructure et microscope a source lumineuse Withdrawn EP1644765A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10331906A DE10331906B4 (de) 2003-07-15 2003-07-15 Lichtquelle mit einem Mikrostruktuierten optischen Element und Mikroskop mit Lichtquelle
PCT/EP2004/051515 WO2005006042A1 (fr) 2003-07-15 2004-07-15 Source lumineuse pourvue d'un element optique microstructure et microscope a source lumineuse

Publications (1)

Publication Number Publication Date
EP1644765A1 true EP1644765A1 (fr) 2006-04-12

Family

ID=34041860

Family Applications (1)

Application Number Title Priority Date Filing Date
EP04766239A Withdrawn EP1644765A1 (fr) 2003-07-15 2004-07-15 Source lumineuse pourvue d'un element optique microstructure et microscope a source lumineuse

Country Status (7)

Country Link
US (1) US20060165359A1 (fr)
EP (1) EP1644765A1 (fr)
JP (1) JP2009513994A (fr)
CN (1) CN1823288A (fr)
DE (1) DE10331906B4 (fr)
RU (1) RU2006104323A (fr)
WO (1) WO2005006042A1 (fr)

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US6831781B2 (en) * 1998-02-26 2004-12-14 The General Hospital Corporation Confocal microscopy with multi-spectral encoding and system and apparatus for spectroscopically encoded confocal microscopy
DE102004055321B4 (de) * 2004-11-16 2020-12-24 Leica Microsystems Cms Gmbh Optische Anordnung für ein Mikroskop und ein Mikroskop
US7519253B2 (en) 2005-11-18 2009-04-14 Omni Sciences, Inc. Broadband or mid-infrared fiber light sources
KR101584102B1 (ko) * 2007-12-18 2016-01-12 코닌클리케 필립스 엔.브이. 광 결정 ｌｅｄ
DE202009007789U1 (de) 2009-06-03 2009-08-20 Carl Zeiss Microimaging Gmbh Breitbandige Lichtquelle und Mikroskop
DE102009024941A1 (de) * 2009-06-09 2010-12-23 Carl Zeiss Surgical Gmbh Beleuchtungsvorrichtung und medizinisch-optisches Beobachtungsgerät

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JP2505892B2 (ja) * 1989-08-03 1996-06-12 浜松ホトニクス株式会社 パラメトリツクパルスレ―ザ
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DE19612846C2 (de) * 1996-03-30 2000-04-20 Zeiss Carl Jena Gmbh Anordnung zur Erzeugung eines definierten Farblängsfehlers in einem konfokalen mikroskopischen Strahlengang
DE19758744C2 (de) * 1997-01-27 2003-08-07 Zeiss Carl Jena Gmbh Laser-Scanning-Mikroskop
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Also Published As

Publication number Publication date
DE10331906A1 (de) 2005-02-17
DE10331906B4 (de) 2005-06-16
US20060165359A1 (en) 2006-07-27
RU2006104323A (ru) 2007-09-20
JP2009513994A (ja) 2009-04-02
WO2005006042A1 (fr) 2005-01-20
CN1823288A (zh) 2006-08-23

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