EP1637227B1 - Sealing device for covering a filtration unit - Google Patents

Sealing device for covering a filtration unit Download PDF

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Publication number
EP1637227B1
EP1637227B1 EP05017680.9A EP05017680A EP1637227B1 EP 1637227 B1 EP1637227 B1 EP 1637227B1 EP 05017680 A EP05017680 A EP 05017680A EP 1637227 B1 EP1637227 B1 EP 1637227B1
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EP
European Patent Office
Prior art keywords
filter device
plate
mat
reaction vessels
filter
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EP05017680.9A
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German (de)
French (fr)
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EP1637227A1 (en
Inventor
Kurt Harnack
Kirsten Schicke
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Eppendorf SE
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Eppendorf SE
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Publication of EP1637227A1 publication Critical patent/EP1637227A1/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration

Definitions

  • the invention relates to a device according to the preamble of claim 1.
  • Generic devices are used in the parallel work-up of several liquid samples by means of vacuum filtration in filter devices, which have a plurality of reaction vessels formed together in a two-dimensional composite.
  • Typical representatives of such filter devices are, for example, microtiter filter plates, which, like conventional microtiter plates, may, for example, have 96 or 384 reaction vessels, also referred to as cavities.
  • the reaction vessels provided in microtiter filter plates have an upper and a lower opening and a filter arranged between the openings.
  • first sample liquid is pipetted into the reaction vessels of the filter device.
  • the lower openings are then vacuumed and the sample liquid is drawn through the filters.
  • a collecting device for the liquid is provided below the filter device.
  • microtiter filter plates e.g. a microtiter plate for collecting the soaked liquid can be arranged.
  • a vacuum chamber into which the filter device can be used such that the upper openings are substantially freely accessible, while the lower openings are applied to the vacuum generated in the chamber.
  • the upper openings of the unfilled reaction vessels are e.g. be taped.
  • the describes DE 101 42 960 the use of an elastically deformable sealing mat, which is such that it selectively seals only the non-filled reaction vessels in a flat arrangement on the filter device.
  • This effect is essentially due to the fact that in the non-filled reaction vessels after applying the vacuum initially higher suction prevails than in the filled reaction vessels. Due to the higher suction, the mat is selectively sucked to the upper openings of the unfilled reaction vessels and then sealed.
  • the object of the invention is to provide a further device for the selective sealing of portions of filter devices, which in particular enables an improved automated handling.
  • the device according to the invention also has an elastically deformable mat.
  • the mat is arranged flat on the underside of a rigid plate whose dimensions are adapted to the upper surface of the filter device.
  • Plates with adapted to the upper surface of the filter device dimensions are essentially all plates that can be easily arranged on the filter device.
  • these are advantageously plates whose dimensions correspond to the upper surface of the filter device or, in the case of smaller plates, coincide with the upper surface of the filter device with respect to the extension in one of the surface axes so that they can be easily separated from, for example, the Handle gripping device of a workstation.
  • the inventively used, held on the plate mat is such that when the plate is placed on the filter device in sealing engagement in such a way that when applied to the lower openings vacuum between Mat and filter device can not flow air between the upper openings.
  • the mat used according to the invention additionally covers the upper openings of the reaction vessels and then seals them off directly.
  • the mat has a flat surface, d. H. not or only slightly protrudes into the reaction vessels. It is also conceivable, however, for the mat to have projections which seal against the upper inner wall regions of the reaction vessels when the mat is arranged on the filter device.
  • the size of the mat must be adapted to the size of the desired to be covered partial area of the filter device, or in areas where no sealing is desired, the plate provided in the device according to the invention on its underside Also do not wear a mat, or do not cover these areas together with the mat.
  • This embodiment is particularly suitable when the devices according to the invention are to be kept prefabricated for different subareas to be covered. It is conceivable, for example, that the different devices are arranged in defined storage locations of a work station and then automatically selected and processed by the gripping device in a preprogrammed manner depending on the filling state of the filter device to be covered.
  • a generic further variant of the invention provides that the device is individually adaptable to the partial surface areas of the filter device to be covered.
  • mat and plate advantageously correspond in their dimensions to the upper surface of the filter device to be covered. Holes are provided in the mat and in the plate which, when the plate is arranged on the upper surface of the filter means, allow passage of air flow from the top of the plate through the mat into a reaction vessel.
  • a film suitable for a particular partial surface area of the filter device can be selectively selected from a set of films of different sizes and arranged on the upper side of the plate.
  • the film used according to this embodiment may be made relatively thin. Since it is placed on top of the plate, theoretically no further foil holding means is required. In any case, sufficient facilities that prevent slipping of the film.
  • the plate it is conceivable, for example, for the plate to have a number of through bores corresponding to the number of reaction vessels, and to have no through holes in the part surface areas of the filter device to be covered by mats which can be connected to the plate. Again, different mats for different partial surface areas of the filter devices are provided, which are each selected the filling state of the filter device and attached to the plate. It is understood that in this case no permanent connection between the plate and mat is provided, but a detachable.
  • One embodiment provides that both plate and mat match in number and orientation of the through holes with the number and orientation of the reaction vessels in the filter device.
  • the main field of application of the device according to the invention is the suction-tight covering of partial surface areas of microtiter filter plates.
  • the usual application is that the covered partial surface areas comprise one or more of the reaction vessels usually arranged in rows next to one another in such plates.
  • the covered partial surface areas comprise one or more of the reaction vessels usually arranged in rows next to one another in such plates.
  • another more individualized coverage is conceivable. It can not be ruled out that several non-contiguous surface areas can be covered.
  • the invention is not limited to the format of the microtiter filter plates. At least theoretically, other filter devices are conceivable in which a plurality of reaction vessels are formed. These filter devices should also be detected.
  • plate used in the description is not limiting. It should also be included, e.g. lid-like facilities.
  • the plate used according to the invention has a weight which is sufficient to sufficiently press the mat arranged thereon after arrangement on the filter device, so that the desired sealing effect can occur after application of the vacuum.
  • the plate should be so heavy be that slipping is prevented by any vibrations of the vacuum pump.
  • the plate can be made of steel, aluminum or brass, to name just a few examples.
  • the plate may, but need not, have means to adjust it on the filter device.
  • means to adjust it on the filter device In particular, in automatic handling in work stations such facilities are not mandatory.
  • the mat used according to the invention may consist of silicone or rubber, for example.
  • mat material and thickness of the mat are to be selected so that when the mat is placed on the filter device and applied vacuum either the openings of the covered reaction vessels are sealed directly, or only one directed through a through hole in the mat air flow in each case an opening is possible , It is optimal if the mat already seals after applying the vacuum.
  • Mats are also suitable, however, which, in addition to the applied vacuum, require slight pressure for the production of the sealing effect, which can be exerted, for example, by the weight of the plate lying on the mat or else manually or by a gripping device of a workstation.
  • Suitable are all common elastomers, such as rubber or silicone, to name only two examples, in particular Shore A hardnesses between 40-60.
  • Suitable thicknesses are, for example, in the range between 0.5 mm and 1 mm. Of course, but other hardnesses or thicknesses can be selected.
  • Fig. 1 shows an embodiment of the device 10 according to the invention in on a filter device (in the case shown, a microtiter filter plate 11), attached state.
  • the microtiter filter plate 11 has a plurality of reaction vessels 12a-f, of which, for reasons of clarity, only the vessels 12a, c and f are designated.
  • the reaction vessels 12 a - f each have an upper opening 13 and a lower opening 14. Furthermore, a filter 15 is provided in the region of the lower opening in the reaction vessels 12 a-f.
  • the device 10 has a plate 16 which, in the case shown, is designed as a cover which can be placed on the microtiter filter plate 11.
  • the plate 16 may have a through bore 22 through which air can flow after applying a vacuum. It is also conceivable, for example, to use plates that do not rest completely sealed on the microtiter filter plate.
  • an elastically deformable mat 18 is arranged on the plate 16, the suction-tight in a partial surface area 19 of the filter plate 11 to areas 19 which surrounds the openings 13 of the unfilled reaction vessels 12 a - 12 c and seals them.
  • the microtiter filter plate 31 is inserted into a vacuum chamber 50, which is only indicated, in which a microtiter plate 60 arranged underneath the microtiter filter plate 11 is additionally provided for collecting the filtered liquid. It has been dispensed with a detailed illustration of the vacuum chamber, since such chambers are commercially available and familiar to the expert.
  • FIG. 2 Another embodiment of the invention shows Fig. 2 , One recognizes here an embodiment of the device 30 according to the invention, again in a state arranged on a microtiter filter plate 31.
  • the microtiter filter plate 31 is in turn inserted into the vacuum chamber 50 shown only indicated.
  • the microtiter filter plate 31 has reaction vessels 32 a - g, of which the vessels 32 e - 32 g are filled with liquid 40.
  • the vessels 32 a - g have upper openings 33 and lower openings 34, wherein in the region of the lower opening 34, a filter 35 is provided.
  • the inventive device 30 has a mat 48 which is arranged on the underside of a plate 46.
  • the mat 48 and the plate 46 are respectively with the upper openings 33 of the reaction vessels 32 a - g aligned through holes 49 and 50 are provided, which allow in the region of the reaction vessel 32 e - 32 g, the passage of an air flow from the top of the plate 46 into the reaction vessels.
  • the through holes 50 in the plate 46 are covered by a suction-tight on the top of the plate 46 arranged film 51.
  • the passage of air into these reaction vessels is therefore not possible, wherein in turn, after applying a vacuum, the undesired side air effect can be avoided in a simple manner.
  • the foil 51 can theoretically be arranged on the plate 46 without fastening means. In the case shown, one recognizes one of a plurality of thorns 52 which are intended to prevent a lateral slippage of the film 51.
  • the mat is in sealing contact both on the surface of the filter device in regions 59 surrounding the openings and on the underside of the plate.
  • the film 51 can be replaced in a particularly simple manner against other films with different dimensions.
  • FIGS. 3a-c shown, each having a plate 56 arranged thereon, held on domes 72, different films 61a-d shows.
  • Fig. 1 and Fig. 2 which show a basic structure, have the in Figs. 3a-c shown plates through holes 70, which correspond in number and arrangement to a conventional 96-well microtiter plate format.

Description

Die Erfindung bezieht sich auf eine Vorrichtung nach dem Oberbegriff des Anspruches 1.The invention relates to a device according to the preamble of claim 1.

Gattungsgemäße Vorrichtungen kommen zum Einsatz bei der parallelen Aufarbeitung mehrerer flüssiger Proben mittels Vakuum-Filtration in Filtereinrichtungen, die mehrere in einem flächigen Verbund gemeinsam ausgebildete Reaktionsgefäße aufweisen. Typische Vertreter solcher Filtereinrichtungen sind z.B. Mikrotiterfilterplatten, die wie herkömmliche Mikrotiterplatten beispielsweise 96 oder 384 Reaktionsgefäße, auch als Kavitäten bezeichnet, aufweisen können. Im Gegensatz zu Mikrotiterplatten weisen die in Mikrotiterfilterplatten vorgesehenen Reaktionsgefäße eine obere und eine untere Öffnung sowie einen zwischen den Öffnungen angeordneten Filter auf.Generic devices are used in the parallel work-up of several liquid samples by means of vacuum filtration in filter devices, which have a plurality of reaction vessels formed together in a two-dimensional composite. Typical representatives of such filter devices are, for example, microtiter filter plates, which, like conventional microtiter plates, may, for example, have 96 or 384 reaction vessels, also referred to as cavities. In contrast to microtiter plates, the reaction vessels provided in microtiter filter plates have an upper and a lower opening and a filter arranged between the openings.

Bei einer üblichen Aufarbeitung wird zunächst Probeflüssigkeit in die Reaktionsgefäße der Filtereinrichtung pipettiert. Die unteren Öffnungen werden dann mit Vakuum beaufschlagt und die Probeflüssigkeit durch die Filter gesogen. In der Regel ist unterhalb der Filtereinrichtung eine Auffangeinrichtung für die Flüssigkeit vorgesehen. Bei Aufarbeitungen mit Mikrotiterfilterplatten kann z.B. eine Mikrotiterplatte zum Auffangen der durchgesogenen Flüssigkeit angeordnet werden.In a conventional workup, first sample liquid is pipetted into the reaction vessels of the filter device. The lower openings are then vacuumed and the sample liquid is drawn through the filters. As a rule, a collecting device for the liquid is provided below the filter device. For work-up with microtiter filter plates, e.g. a microtiter plate for collecting the soaked liquid can be arranged.

Üblicherweise verwendet man zur Erzeugung des Vakuums eine Vakuumkammer, in die die Filtereinrichtung dergestalt eingesetzt werden kann, daß die oberen Öffnungen im wesentlichen frei zugänglich sind, während die unteren Öffnungen mit dem in der Kammer erzeugten Vakuum beaufschlagt werden.Typically, one uses to create the vacuum, a vacuum chamber into which the filter device can be used such that the upper openings are substantially freely accessible, while the lower openings are applied to the vacuum generated in the chamber.

Probleme stellen sich immer dann ein, wenn nicht alle Reaktionsgefäße der Filtereinrichtung mit Probeflüssigkeit befüllt sind. Die ungefüllten Reaktionsgefäße können dann einen Nebenlufteffekt verursachen, der das angelegte Vakuum beeinträchtigt, d.h. der erforderliche Unterdruck wird nicht erreicht, weil die leeren Gefäße einen geringeren Strömungswiderstand haben.Problems always arise when not all the reaction vessels of the filter device are filled with sample liquid. The unfilled reaction vessels may then cause a side air effect that affects the applied vacuum, i. the required negative pressure is not reached, because the empty vessels have a lower flow resistance.

Zur Vermeidung des Nebenlufteffektes ist bekannt, daß die oberen Öffnungen der nicht befüllten Reaktionsgefäße z.B. abgeklebt werden.To avoid the side air effect, it is known that the upper openings of the unfilled reaction vessels are e.g. be taped.

Alternativ beschreibt die DE 101 42 960 den Einsatz einer elastisch verformbaren Dichtmatte, die so beschaffen ist, daß sie bei flächiger Anordnung auf der Filtereinrichtung selektiv nur die nicht befüllten Reaktionsgefäße saugdicht verschließt. Dieser Effekt kommt im wesentlichen dadurch zustande, daß in den nicht befüllten Reaktionsgefäßen nach Anlegen des Vakuums zunächst eine höhere Saugwirkung herrscht als in den befüllten Reaktionsgefäßen. Aufgrund der höheren Saugwirkung wird die Matte selektiv an die oberen Öffnungen der nicht befüllten Reaktionsgefäße gesogen und versiegelt diese dann.Alternatively, the describes DE 101 42 960 the use of an elastically deformable sealing mat, which is such that it selectively seals only the non-filled reaction vessels in a flat arrangement on the filter device. This effect is essentially due to the fact that in the non-filled reaction vessels after applying the vacuum initially higher suction prevails than in the filled reaction vessels. Due to the higher suction, the mat is selectively sucked to the upper openings of the unfilled reaction vessels and then sealed.

Aufgabe der Erfindung ist es, eine weitere Vorrichtung zur selektiven Abdichtung von Teilbereichen von Filtereinrichtungen bereitzustellen, die insbesondere ein verbessertes automatisiertes Handling ermöglicht.The object of the invention is to provide a further device for the selective sealing of portions of filter devices, which in particular enables an improved automated handling.

Gelöst wird die Aufgabe mit einer Vorrichtung, die die kennzeichnenden Merkmale des Anspruches 1 aufweist. Vorteilhafte Ausgestaltungen der Erfindung sind in den Unteransprüchen angegeben.The problem is solved with a device having the characterizing features of claim 1. Advantageous embodiments of the invention are specified in the subclaims.

Wie im Stand der Technik, so weist auch die erfindungsgemäße Vorrichtung eine elastisch verformbare Matte auf.As in the prior art, the device according to the invention also has an elastically deformable mat.

Erfindungsgemäß ist vorgesehen, dass die Matte flächig auf der Unterseite einer starren Platte angeordnet ist, deren Abmessungen der oberen Fläche der Filtereinrichtung angepaßt sind. Platten mit an die obere Fläche der Filtereinrichtung angepaßten Abmessungen sind im wesentlichen alle Platten, die sich problemlos auf der Filtereinrichtung anordnen lassen. Im Hinblick auf das gewünschte automatische Handling handelt es sich vorteilhafterweise um Platten, deren Abmessungen der oberen Fläche der Filtereinrichtung entsprechen oder bei kleineren Platten bezüglich der Erstreckung in einer der Flächenachsen mit der oberen Fläche der Filtereinrichtung übereinstimmen, damit sie sich in einfacher Weise von z.B der Greifeinrichtung einer Work-Station handhaben lassen.According to the invention it is provided that the mat is arranged flat on the underside of a rigid plate whose dimensions are adapted to the upper surface of the filter device. Plates with adapted to the upper surface of the filter device dimensions are essentially all plates that can be easily arranged on the filter device. With regard to the desired automatic handling, these are advantageously plates whose dimensions correspond to the upper surface of the filter device or, in the case of smaller plates, coincide with the upper surface of the filter device with respect to the extension in one of the surface axes so that they can be easily separated from, for example, the Handle gripping device of a workstation.

Die erfindungsgemäß eingesetzte, an der Platte gehaltene Matte ist so beschaffen, daß sie bei Anordnung der Platte auf der Filtereinrichtung in dichtenden Eingriff gelangt dergestalt, daß bei an die unteren Öffnungen angelegtem Vakuum zwischen Matte und Filtereinrichtung keine Luft zwischen den oberen Öffnungen strömen kann.The inventively used, held on the plate mat is such that when the plate is placed on the filter device in sealing engagement in such a way that when applied to the lower openings vacuum between Mat and filter device can not flow air between the upper openings.

Im einfachsten Fall deckt die erfindungsgemäß eingesetzte Matte zusätzlich die oberen Öffnungen der Reaktionsgefäße ab und dichtet diese dann direkt ab.In the simplest case, the mat used according to the invention additionally covers the upper openings of the reaction vessels and then seals them off directly.

Im Hinblick auf das Kontaminationsrisiko ist vorteilhaft vorgesehen, dass die Matte eine plane Oberfläche aufweist, d. h. nicht oder nur geringfügig in die Reaktionsgefäße hineinragt. Denkbar ist aber auch, dass die Matte Vorsprünge aufweist, die sich bei Anordnung der Matte auf der Filtereinrichtung gegen die oberen Innenwandbereiche der Reaktionsgefäße dichtend anlegen.With regard to the risk of contamination is advantageously provided that the mat has a flat surface, d. H. not or only slightly protrudes into the reaction vessels. It is also conceivable, however, for the mat to have projections which seal against the upper inner wall regions of the reaction vessels when the mat is arranged on the filter device.

Es versteht sich, dass in der oben beschriebenen Ausgestaltung die Größe der Matte an die Größe des gewünschten abzudeckenden Teilflächenbereiches der Filtereinrichtung angepaßt werden muss, bzw. in Bereichen, in denen keine Abdichtung gewünscht ist, würde die in der erfindungsgemäßen Vorrichtung vorgesehene Platte auf ihrer Unterseite auch keine Matte tragen, bzw diese Bereiche gemeinsam mit der Matte nicht abdecken.It is understood that in the embodiment described above, the size of the mat must be adapted to the size of the desired to be covered partial area of the filter device, or in areas where no sealing is desired, the plate provided in the device according to the invention on its underside Also do not wear a mat, or do not cover these areas together with the mat.

Diese Ausgestaltung bietet sich insbesondere dann an, wenn die erfindungsgemäßen Vorrichtungen für unterschiedliche abzudeckende Teilflächenbereiche vorgefertigt bereitgehalten werden sollen. Vorstellbar ist z.B., daß man die unterschiedlichen Vorrichtungen in definierten Aufbewahrungsorten einer Work-Station anordnet und diese dann in Abhängigkeit von dem Befüllungszustand der abzudeckenden Filtereinrichtung vorprogrammiert automatisch von der Greifeinrichtung gewählt und prozessiert werden.This embodiment is particularly suitable when the devices according to the invention are to be kept prefabricated for different subareas to be covered. It is conceivable, for example, that the different devices are arranged in defined storage locations of a work station and then automatically selected and processed by the gripping device in a preprogrammed manner depending on the filling state of the filter device to be covered.

Die oben beschriebenen Ausgestaltungen eignen sich insbesondere für den Massendurchsatz.The embodiments described above are particularly suitable for mass flow.

Für andere Anwendungsfälle sieht eine gattungsgemäße weitere Variante der Erfindung vor, daß die Vorrichtung individuell an die abzudeckenden Teilflächenbereiche der Filtereinrichtung anpaßbar ist. Bei dieser Variante entsprechen Matte und Platte vorteilhafterweise in ihren Abmessungen der oberen Fläche der abzudeckenden Filtereinrichtung. In der Matte und in der Platte sind Durchbohrungen vorgesehen, die bei Anordnung der Platte auf der oberen Fläche der Filtereinrichtung jeweils das Durchtreten eines Luftstroms von der Oberseite der Platte durch die Matte in ein Reaktionsgefäß erlauben.For other applications, a generic further variant of the invention provides that the device is individually adaptable to the partial surface areas of the filter device to be covered. In this variant, mat and plate advantageously correspond in their dimensions to the upper surface of the filter device to be covered. Holes are provided in the mat and in the plate which, when the plate is arranged on the upper surface of the filter means, allow passage of air flow from the top of the plate through the mat into a reaction vessel.

Zusätzlich ist eine auf der Oberseite der Platte anordenbare Folie vorgesehen, mit der sich die Durchbohrungen in der Platte saugdicht abdecken lassen. Selbstverständlich ist auch hier wiederum vorgesehen, daß aus einem Set unterschiedlich großer Folien wahlweise eine für einen bestimmten Teilflächenbereich der Filtereinrichtung geeignete Folie ausgewählt und auf der Oberseite der Platte angeordnet werden kann.In addition, a can be arranged on the top of the plate foil, with which the holes in the plate can cover absorbent. Of course, it is again provided here that a film suitable for a particular partial surface area of the filter device can be selectively selected from a set of films of different sizes and arranged on the upper side of the plate.

Diese Variante ermöglicht in besonders eleganter Weise eine Anpassung der erfindungsgemäßen Vorrichtung an die Größe des abzudichtenden Flächenbereiches. Im Gegensatz zu der auf einer Unterseite der Platte vorgesehenen Matte kann die gemäß dieser Ausgestaltung eingesetzte Folie relativ dünn ausgebildet sein. Da sie auf der Oberseite der Platte angeordnet wird, ist theoretisch überhaupt keine weitere die Folie haltende Einrichtung erforderlich. Auf jeden Fall reichen Einrichtungen, die ein Verrutschen der Folie verhindern.This variant makes it possible in a particularly elegant manner to adapt the device according to the invention to the size of the area to be sealed. In contrast to the mat provided on a lower side of the plate, the film used according to this embodiment may be made relatively thin. Since it is placed on top of the plate, theoretically no further foil holding means is required. In any case, sufficient facilities that prevent slipping of the film.

Bei dieser Variante ist z.B. denkbar, daß die Platte eine der Anzahl der Reaktionsgefäße entsprechende Anzahl von Durchbohrungen aufweist, und die mit mit der Platte verbindbare Matten in den abzudeckenden Teilflächenbereichen der Filtereinrichtung keine Durchbohrungen aufweist. Auch hier können unterschiedliche Matten für unterschiedliche Teilflächenbereiche der Filtereinrichtungen vorgesehen werden, die jeweils dem Befüllzustand der Filtereinrichtung ausgewählt und an der Platte befestigt werden. Es versteht sich, daß in diesem Fall keine dauerhafte Verbindung zwischen Platte und Matte vorgesehen wird, sondern eine lösbare.In this variant, it is conceivable, for example, for the plate to have a number of through bores corresponding to the number of reaction vessels, and to have no through holes in the part surface areas of the filter device to be covered by mats which can be connected to the plate. Again, different mats for different partial surface areas of the filter devices are provided, which are each selected the filling state of the filter device and attached to the plate. It is understood that in this case no permanent connection between the plate and mat is provided, but a detachable.

Eine Ausgestaltung sieht vor, daß sowohl Platte als auch Matte bezüglich Anzahl und Ausrichtung der Durchbohrungen mit der Anzahl und Ausrichtung der Reaktionsgefäße in der Filtereinrichtung übereinstimmen. Bei dieser Ausgestaltung istOne embodiment provides that both plate and mat match in number and orientation of the through holes with the number and orientation of the reaction vessels in the filter device. In this embodiment

Es ist davon auszugehen, dass das Haupteinsatzgebiet der erfindungsgemäßen Vorrichtung die saugdichte Abdeckung von Teilflächenbereichen von Mikrotiterfilterplatten ist. Der übliche Anwendungsfall ist, daß die abgedeckten Teilflächenbereiche eine bzw. mehrere der in solchen Platten üblicherweise in Reihen nebeneinander angeordneten Reaktionsgefäßen umfassen. Denkbar ist natürlich auch eine weitere individuellere Abdeckung. Nicht auszuschließen ist, daß mehrere nicht zusammenhängende Teilflächenbereiche abgedeckt werden können.It can be assumed that the main field of application of the device according to the invention is the suction-tight covering of partial surface areas of microtiter filter plates. The usual application is that the covered partial surface areas comprise one or more of the reaction vessels usually arranged in rows next to one another in such plates. Of course, another more individualized coverage is conceivable. It can not be ruled out that several non-contiguous surface areas can be covered.

Selbstverständlich ist die Erfindung nicht auf das Format der Mikrotiterfilterplatten beschränkt. Es sind zumindest theoretisch auch andere Filtereinrichtungen denkbar, in denen mehrere Reaktionsgefäße ausgebildet sind. Auch diese Filtereinrichtungen sollen erfaßt werden.Of course, the invention is not limited to the format of the microtiter filter plates. At least theoretically, other filter devices are conceivable in which a plurality of reaction vessels are formed. These filter devices should also be detected.

Der im Rahmen der Beschreibung verwendete Begriff "Platte" ist nicht limitierend. Umfaßt werden sollen damit auch z.B. deckelähnliche Einrichtungen.The term "plate" used in the description is not limiting. It should also be included, e.g. lid-like facilities.

Vorteilhafterweise weist die erfindungsgemäß eingesetzte Platte ein Gewicht auf, das ausreicht, die daran angeordnete Matte nach Anordnung auf der Filtereinrichtung ausreichend fest anzudrücken, damit der gewünschte Dichteffekt nach Anlegen des Vakuums eintreten kann. In wesentlichen sollte die Platte so schwe-re sein, dass ein Verrutschen durch eventuelle Vibrationen der Vakuumpumpe verhindert wird. Die Platte kann z.B. aus Stahl, Aluminium oder Messing bestehen, um nur einige Beispiele zu nennen.Advantageously, the plate used according to the invention has a weight which is sufficient to sufficiently press the mat arranged thereon after arrangement on the filter device, so that the desired sealing effect can occur after application of the vacuum. In essence, the plate should be so heavy be that slipping is prevented by any vibrations of the vacuum pump. The plate can be made of steel, aluminum or brass, to name just a few examples.

Denkbar ist selbstverständlich aber auch, z.B. leichtere Platten ggf. auch aus anderen Materialien einzusetzen, die von sich aus die Matte nicht fest genug andrücken, um den gewünschten Dichteffekt zu gewährleisten, und diese Platten manuell bzw. durch die in Work-Stations eingesetzte Greifeinrichtung so lange anzudrücken, bis der Dichteffekt eingetreten ist.Of course, it is also conceivable, however, e.g. If necessary, also use lighter panels of other materials that do not press the mat firmly enough to ensure the desired sealing effect, and press these panels manually or through the gripping device used in work stations until the sealing effect has occurred is.

Die Platte kann, muß aber nicht, Einrichtungen aufweisen, die sie auf der Filtereinrichtung justieren. Insbesondere beim automatischen Handling in Work-Stations sind solche Einrichtungen nicht zwingend erforderlich.The plate may, but need not, have means to adjust it on the filter device. In particular, in automatic handling in work stations such facilities are not mandatory.

Die erfindungsgemäß eingesetzte Matte kann z.B. aus Silikon oder Gummi bestehen. Grundsätzlich sind Mattenmaterial und Dicke der Matte so zu wählen, dass bei Anordnung der Matte auf der Filtereinrichtung und angelegtem Vakuum entweder die Öffnungen der abgedeckten Reaktionsgefäße direkt abgedichtet werden, oder aber nur ein durch eine Durchbohrung in der Matte gerichteter Luftstrom in jeweils eine Öffnung möglich ist. Optimal ist, wenn die Matte bereits nach Anlegen des Vakuums dichtend anliegt. Geeignet sind aber auch Matten, die für die Herstellung der Dichtwirkung zusätzlich zu dem angelegten Vakuum einen leichten Andruck benötigen, der z.B. durch das Gewicht der auf der Matte liegenden Platte oder aber auch manuell bzw. durch eine Greifeinrichtung einer Workstation ausgeübt werden kann. Geeignet sind alle gängigen Elastomere, wie z.B. Gummi oder Silikon, um nur zwei Beispiele zu nennen, mit insbesondere Shore-A Härten zwischen 40-60. Geeignete Dicken liegen z.B. im Bereich zwischen 0,5 mm und 1mm. Selbstverständlich können aber auch andere Härten bzw. Dicken gewählt werden.The mat used according to the invention may consist of silicone or rubber, for example. Basically, mat material and thickness of the mat are to be selected so that when the mat is placed on the filter device and applied vacuum either the openings of the covered reaction vessels are sealed directly, or only one directed through a through hole in the mat air flow in each case an opening is possible , It is optimal if the mat already seals after applying the vacuum. Mats are also suitable, however, which, in addition to the applied vacuum, require slight pressure for the production of the sealing effect, which can be exerted, for example, by the weight of the plate lying on the mat or else manually or by a gripping device of a workstation. Suitable are all common elastomers, such as rubber or silicone, to name only two examples, in particular Shore A hardnesses between 40-60. Suitable thicknesses are, for example, in the range between 0.5 mm and 1 mm. Of course, but other hardnesses or thicknesses can be selected.

Im Folgenden soll die Erfindung anhand von Zeichnungen näher beschrieben werden. Dabei zeigt:

Fig. 1
ein Ausführungsbeispiel der erfindungsgemäßen Vorrichtung,
Fig. 2
ein weiteres Ausführungsbeispiel und
Fig. 3a-c
Aufsichten auf das Ausführungsbeispiel gemäß Fig. 2 jeweils angepaßt an die Abdichtung unterschiedlicher Teilbereiche.
In the following, the invention will be described in more detail with reference to drawings. Showing:
Fig. 1
an embodiment of the device according to the invention,
Fig. 2
another embodiment and
Fig. 3a-c
Supervision of the embodiment according to Fig. 2 each adapted to the sealing of different sections.

Fig. 1 zeigt eine Ausgestaltung der erfindungsgemäßen Vorrichtung 10 in auf eine Filtereinrichtung (im gezeigten Fall eine Mikrotiterfilterplatte 11), aufgesetztem Zustand. Fig. 1 shows an embodiment of the device 10 according to the invention in on a filter device (in the case shown, a microtiter filter plate 11), attached state.

Die Mikrotiterfilterplatte 11 weist mehrere Reaktionsgefäße 12 a - f auf, von denen aus Übersichtlichkeitsgründen nur die Gefäße 12 a, c und f bezeichnet sind. Die Reaktionsgefäße 12 a - f weisen jeweils eine obere Öffnung 13 und eine untere Öffnung 14 auf. Weiterhin ist im Bereich der unteren Öffnung in den Reaktionsgefäßen 12 a - f ein Filter 15 vorgesehen.The microtiter filter plate 11 has a plurality of reaction vessels 12a-f, of which, for reasons of clarity, only the vessels 12a, c and f are designated. The reaction vessels 12 a - f each have an upper opening 13 and a lower opening 14. Furthermore, a filter 15 is provided in the region of the lower opening in the reaction vessels 12 a-f.

Die erfindungsgemäße Vorrichtung 10 weist eine Platte 16 auf, die im gezeigten Fall als auf die Mikrotiterfilterplatte 11 aufsetzbarer Deckel ausgebildet ist. Die Platte 16 kann dabei eine Durchbohrung 22 aufweisen, durch die nach Anlegen eines Vakuums Luft nachströmen kann. Denkbar ist aber auch z.B. Platten zu verwenden, die nicht vollständig abgedichtet auf der Mikrotiterfilterplatte aufliegen. Auf ihrer Unterseite 17 ist an der Platte 16 eine elastisch verformbare Matte 18 angeordnet, die sich in einem Teilflächenbereich 19 der Filterplatte 11 saugdicht an Bereiche 19 anlegt, die die Öffnungen 13 der nicht befüllten Reaktionsgefäße 12 a - 12 c umgeben und diese abdichtet.The device 10 according to the invention has a plate 16 which, in the case shown, is designed as a cover which can be placed on the microtiter filter plate 11. The plate 16 may have a through bore 22 through which air can flow after applying a vacuum. It is also conceivable, for example, to use plates that do not rest completely sealed on the microtiter filter plate. On its underside 17, an elastically deformable mat 18 is arranged on the plate 16, the suction-tight in a partial surface area 19 of the filter plate 11 to areas 19 which surrounds the openings 13 of the unfilled reaction vessels 12 a - 12 c and seals them.

Die mit Flüssigkeit 20 befüllten Reaktionsgefäße 12 d - f werden von der Matte 18 nicht abgedeckt.The filled with liquid 20 reaction vessels 12 d - f are not covered by the mat 18.

Die Mikrotiterfilterplatte 31 ist in eine nur angedeutet dargestellte Vakuumkammer 50 eingesetzt, in der zusätzlich eine unterhalb der Mikrotiterfilterplatte 11 angeordnete Mikrotiterplatte 60 zum Auffangen der filtrierten Flüssigkeit vorgesehen ist. Es wurde auf eine detaillierte Darstellung der Vakuumkammer verzichtet, da solche Kammern im Markt erhältlich und dem Fachmann geläufig sind.The microtiter filter plate 31 is inserted into a vacuum chamber 50, which is only indicated, in which a microtiter plate 60 arranged underneath the microtiter filter plate 11 is additionally provided for collecting the filtered liquid. It has been dispensed with a detailed illustration of the vacuum chamber, since such chambers are commercially available and familiar to the expert.

Legt man nun ein Vakuum an die unteren Öffnungen 14 der Reaktionsgefäße 12 a - f an, so erfolgt eine Luftströmung nur durch die Gefäße 12 d - f. Auf diese Weise ließe sich der Nebenlufteffekt, der durch nicht verschlossene Reaktionsgefäßen 12 a - c eintreten würde, besonders einfach vermeiden.If a vacuum is now applied to the lower openings 14 of the reaction vessels 12 a - f, an air flow takes place only through the vessels 12 d - f. In this way, it would be particularly easy to avoid the secondary air effect, which would occur through non-closed reaction vessels 12 a - c.

Eine weitere Ausführungsform der Erfindung zeigt Fig. 2. Man erkennt hier eine Ausführungsform der erfindungsgemäßen Vorrichtung 30, wiederum in auf einer Mikrotiterfilterplatte 31 angeordnetem Zustand.Another embodiment of the invention shows Fig. 2 , One recognizes here an embodiment of the device 30 according to the invention, again in a state arranged on a microtiter filter plate 31.

Die Mikrotiterfilterplatte 31 ist wiederum in die nur angedeutet dargestellte Vakuumkammer 50 eingesetzt.The microtiter filter plate 31 is in turn inserted into the vacuum chamber 50 shown only indicated.

Die Mikrotiterfilterplatte 31 weist Reaktionsgefäße 32 a - g auf, von denen die Gefäße 32 e - 32 g mit Flüssigkeit 40 gefüllt sind. Die Gefäße 32 a - g weisen obere Öffnungen 33 und untere Öffnungen 34 auf, wobei im Bereich der unteren Öffnung 34 ein Filter 35 vorgesehen ist.The microtiter filter plate 31 has reaction vessels 32 a - g, of which the vessels 32 e - 32 g are filled with liquid 40. The vessels 32 a - g have upper openings 33 and lower openings 34, wherein in the region of the lower opening 34, a filter 35 is provided.

Die erfindungsgemäße Vorrichtung 30 weist eine Matte 48 auf, die an der Unterseite einer Platte 46 angeordnet ist. In der Matte 48 und der Platte 46 sind jeweils mit den oberen Öffnungen 33 der Reaktionsgefäße 32 a - g fluchtende Durchbohrungen 49 und 50 vorgesehen, die im Bereich der Reaktionsgefäße 32 e - 32 g den Durchtritt eines Luftstroms von der Oberseite der Platte 46 in die Reaktionsgefäße ermöglichen.The inventive device 30 has a mat 48 which is arranged on the underside of a plate 46. In the mat 48 and the plate 46 are respectively with the upper openings 33 of the reaction vessels 32 a - g aligned through holes 49 and 50 are provided, which allow in the region of the reaction vessel 32 e - 32 g, the passage of an air flow from the top of the plate 46 into the reaction vessels.

Im Bereich der Reaktionsgefäße 32 a - 32 d sind die Durchbohrungen 50 in der Platte 46 durch eine auf der Oberseite der Platte 46 angeordnete Folie 51 saugdicht abgedeckt. Der Durchtritt von Luft in diese Reaktionsgefäße ist also nicht möglich, wobei wiederum nach Anlegen eines Vakuums der unerwünschte Nebenlufteffekt in einfacher Weise vermieden werden kann. Die Folie 51 kann theoretisch ohne Befestigungsmittel auf der Platte 46 angeordnet werden. Im gezeigten Fall erkennt man einen von mehreren Dornen 52, die ein seitliches Verrutschen der Folie 51 verhindern sollen. Im gezeigten Ausführungsbeispiel liegt die Matte sowohl an der Oberfläche der Filtereinrichtung in die Öffnungen umgebenden Bereichen 59 als auch an der Unterseite der Platte dichtend an.In the area of the reaction vessels 32 a - 32 d, the through holes 50 in the plate 46 are covered by a suction-tight on the top of the plate 46 arranged film 51. The passage of air into these reaction vessels is therefore not possible, wherein in turn, after applying a vacuum, the undesired side air effect can be avoided in a simple manner. The foil 51 can theoretically be arranged on the plate 46 without fastening means. In the case shown, one recognizes one of a plurality of thorns 52 which are intended to prevent a lateral slippage of the film 51. In the exemplary embodiment shown, the mat is in sealing contact both on the surface of the filter device in regions 59 surrounding the openings and on the underside of the plate.

Es versteht sich, daß die Folie 51 in besonders einfacher Weise gegen andere Folien mit anderen Abmessungen ausgetauscht werden kann. Dies ist in den Figuren 3a-c dargestellt, die jeweils eine Platte 56 mit darauf angeordneten, an Domen 72 gehaltenen, unterschiedlichen Folien 61a-d zeigt. Im Gegensatz zu den Darstellungen in Fig. 1 und Fig. 2, die einen prinzipiellen Aufbau zeigen, weisen die in Figs. 3a-c gezeigten Platten Durchbohrungen 70 auf, die in Anzahl und Anordnung einem üblichen 96er-Mikrotiterfilterplattenformat entsprechen.It is understood that the film 51 can be replaced in a particularly simple manner against other films with different dimensions. This is in the FIGS. 3a-c shown, each having a plate 56 arranged thereon, held on domes 72, different films 61a-d shows. In contrast to the representations in Fig. 1 and Fig. 2 , which show a basic structure, have the in Figs. 3a-c shown plates through holes 70, which correspond in number and arrangement to a conventional 96-well microtiter plate format.

Man erkennt, dass sich bereits mit den gezeigten 4 unterschiedlich grossen Folien 61 a-d eine Vielzahl unterschiedlicher Teilflächenbereiche abdecken lässt.It can be seen that a large number of different partial surface areas can already be covered with the 4 differently sized films 61 a-d shown.

Claims (7)

  1. Filter device having an apparatus for covering it in a suction-tight manner, wherein the filter device has reaction vessels (12a-f) which are formed jointly in a sheet-like composite, said reaction vessels (12a-f) each having an upper (13) and a lower (14) orifice and the upper orifices of said reaction vessels (12a-f) being located substantially in an upper surface of the filter device, and the apparatus has an elastically deformable mat (18) that is arrangeable flat on the filter device, wherein when the mat is arranged on the upper surface of the filter device, reaction vessels located only in a partial surface region of the filter device are covered in a suction-tight manner, characterized in that the mat (18) is arranged flat on the underside (17) of a rigid plate (16), the dimensions of which are matched to the upper surface of the filter device (11), and the mat (18) has a size that matches the size of the desired partial surface region, to be covered, of the filter device and is designed such that when the plate (16) is arranged on the upper surface of the filter device (11), the mat (18) passes into sealing engagement with in each case upper wall regions of the upper orifices (13, 33) and/or regions (19, 59) of the filter device (11) that surround the upper orifices, in such a way that when a vacuum is applied between the mat (18) and the filter device (11) at the lower orifices (14), no air can flow between the upper orifices (13).
  2. Filter device according to Claim 1, characterized in that the dimensions of the plate (16, 46, 56) correspond to the upper surface of the filter device (11, 31).
  3. Filter device having an apparatus for covering it in a suction-tight manner, wherein the filter device has reaction vessels (32a-g) which are formed jointly in a sheet-like composite, said reaction vessels (32a-g) each having an upper (33) and a lower (34) orifice and the upper orifices of said reaction vessels (32a-g) being located substantially in an upper surface of the filter device, and the apparatus has an elastically deformable mat (48) that is arrangeable flat on the filter device, wherein when the mat is arranged on the upper surface of the filter device, reaction vessels located only in a partial surface region of the filter device are covered in a suction-tight manner, characterized in that the dimensions of the mat (48) correspond to the upper surface of the filter device (31), wherein through-holes (49, 50, 70) are provided in the mat (48) and the plate (46, 56), said through-holes (49, 50, 70) allowing in each case a flow of air to pass through the mat (48) into a reaction vessel (32e-g) from the top side of the plate (46, 56) when the plate (46, 56) is arranged on the upper surface of the filter device (31), wherein a film (51, 61a-d) which is arrangeable on the top side of the plate (46, 56) is additionally provided, it being possible to cover the through-holes (50, 70) in the plate (46, 56) in a suction-tight manner by way of said film (51, 61a-d).
  4. Filter device according to Claim 3, characterized in that the dimensions of the plate (46, 56) correspond to the upper surface of the filter device (31).
  5. Filter device according to Claim 3 or 4, characterized in that the number and arrangement of through-holes (49, 50) in the mat (48) and the plate (46) correspond to the number and arrangement of upper orifices (33) in the reaction vessels (32a-32g) in the filter device (31).
  6. Filter device according to Claim 5, characterized in that a set of films (61a-d) of different sizes is provided, it being possible to arrange said films (61a-d) optionally on the top side of the plate (56).
  7. Filter device according to one of the preceding claims, characterized in that the filter device (11, 31) is a microtitre filter plate.
EP05017680.9A 2004-09-15 2005-08-13 Sealing device for covering a filtration unit Active EP1637227B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102004045054A DE102004045054A1 (en) 2004-09-15 2004-09-15 Device for the suction-tight covering of a filter device

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EP1637227A1 EP1637227A1 (en) 2006-03-22
EP1637227B1 true EP1637227B1 (en) 2013-10-02

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EP (1) EP1637227B1 (en)
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CN103100247B (en) * 2013-02-02 2015-06-03 苏州晶云药物科技有限公司 Vacuum filtration device

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EP1637227A1 (en) 2006-03-22
US20070084777A1 (en) 2007-04-19
DE102004045054A1 (en) 2006-03-30
US20060054546A1 (en) 2006-03-16

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