EP1614468B1 - Pipette évitant la formation de goutte et son mode de fonctionnement - Google Patents

Pipette évitant la formation de goutte et son mode de fonctionnement Download PDF

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Publication number
EP1614468B1
EP1614468B1 EP04015682A EP04015682A EP1614468B1 EP 1614468 B1 EP1614468 B1 EP 1614468B1 EP 04015682 A EP04015682 A EP 04015682A EP 04015682 A EP04015682 A EP 04015682A EP 1614468 B1 EP1614468 B1 EP 1614468B1
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EP
European Patent Office
Prior art keywords
liquid
gas
vessel
pressure
gas pressure
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Application number
EP04015682A
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German (de)
English (en)
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EP1614468A1 (fr
Inventor
Renato Nay
Markus Bentz
René Ackermann
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Hamilton Bonaduz AG
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Hamilton Bonaduz AG
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Priority to EP04015682A priority Critical patent/EP1614468B1/fr
Priority to ES04015682T priority patent/ES2274349T3/es
Priority to AT04015682T priority patent/ATE348659T1/de
Priority to DE502004002377T priority patent/DE502004002377D1/de
Publication of EP1614468A1 publication Critical patent/EP1614468A1/fr
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Publication of EP1614468B1 publication Critical patent/EP1614468B1/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0227—Details of motor drive means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0615—Loss of fluid by dripping
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/146—Employing pressure sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/148—Specific details about calibrations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/024—Storing results with means integrated into the container

Definitions

  • the present invention relates to a method for preventing drop losses in remplisstechniksdosiervortechniken.
  • a device of the type mentioned is known from DE 44 21 303 A1.
  • a pipetting device which sucks in or expels an amount of liquid into a portion of a pipetting tip. This is done by changing the gas pressure of a gas trapped between a piston, cylinder walls and the liquid.
  • the pressure of the trapped gas and the prevailing ambient pressure are measured. From the measured values is under Taking into account the geometric shape of the cylinder and the pipette tip calculates a correction value in order to obtain as accurately as possible the distance to be traveled by the piston as a setpoint for the control of the Koblen Gay. The controller then initiates movement of the piston based on the corrected setpoint.
  • DE 44 21 303 A1 be thought to monitor the pressure of the gas between the piston and the amount of liquid between the termination of the fluid intake and the beginning of the liquid delivery to determine leaks on the pipette or the like.
  • WO 97/02893 A1 discloses a method and a device for correcting a temperature-dependent error during the metering of a liquid from a pipette.
  • the known device comprises two chambers connected in series to one another by a gas passage, namely a first chamber in the pipetting tip and a second chamber in the piston-cylinder system to which the pipetting tip is connected.
  • the pipetting tip which is provided with an opening, is immersed in it to absorb liquid.
  • a vessel wall of the second chamber is formed by a movable piston.
  • the second chamber is complete, the first chamber at least partially filled with a gas. The amount of gas is trapped between the piston and liquid in the two chambers.
  • WO 97/02893 A1 proposes to measure the change in temperature of the gas flowing from the first to the second chamber when the liquid is taken up by the piston movement and the change in volume caused by the piston movement in the second chamber to correct based on the measured temperature change during the liquid acquisition process. This is provided at least one temperature sensor.
  • the method known from WO 97/02893 A1 merely serves to correct the piston movement during fluid intake.
  • EP 0 747 689 B1 shows an apparatus and a method for withdrawing a liquid from a tightly closed container.
  • the tightly closed container contains a quantity of gas.
  • the gas pressure inside the container is monitored by a pressure sensor. Before that, the gas pressure inside the sealed vessel is brought to ambient pressure by piercing the seal with a hollow needle.
  • the present application is based on the following problem:
  • the liquid In vessels in which an amount of liquid is delivered or received by varying the pressure of a gas trapped by vessel walls and the liquid, the liquid is kept in the vessel for a considerable time between a liquid receiving operation and a liquid discharging operation, such as to overcome transport routes. During this time, the pressure difference between the ambient pressure and the pressure of the trapped gas as well as frictional and adhesive forces acting between the liquid and the wetted wall maintain the liquid in the vessel. In this case, the pressure difference between the ambient pressure and the gas pressure in the interior of the vessel has the largest share of the force holding the liquid in the vessel.
  • the pressure of the gas trapped in the vessel may change due to evaporation or due to temperature compensation processes.
  • Evaporates for example, absorbed liquid, so the increases Gas pressure in the vessel.
  • the gas pressure usually increases more than the weight of the not yet evaporated liquid decreases.
  • the described processes can lead to the unwanted increased gas pressure, a part of the liquid absorbed amount is undesirably ejected from the vessel. The ejected liquid then drips off the vessel. As a result, undesirably erroneous amounts of liquid can be dispensed despite first correctly taken in liquid quantities.
  • long transport distances can be covered or the liquid metering device can be stopped without loss of metered liquid after receiving the liquid for important short-term required interventions.
  • control device is a control device which is adapted to control at least during a control period between liquid intake and liquid delivery, the gas pressure changing device in response to the detected state variable such that the actual gas pressure in Vessel is held during the control period substantially at a predetermined target gas pressure.
  • the gas pressure is maintained at a predetermined target gas pressure in a control period, which control period comprises a time period in the first half, preferably in the first quarter, between the end time of the liquid receiving device and the start time of the liquid dispensing operation.
  • control period comprises a time period in the first half, preferably in the first quarter, between the end time of the liquid receiving device and the start time of the liquid dispensing operation.
  • the evaporation and temperature compensation processes are fastest and cause a faster change in gas pressure compared to a later period between fluid intake and fluid delivery. It is therefore further advantageous for the reliable prevention of dripping when the control period comprises the first quarter or particularly advantageously the first half of the period lying between the end time of the liquid receiving operation and the start time of the liquid dispensing operation.
  • control period covers the entire time between the end time of the liquid intake operation and the start time of the liquid discharge operation Period covers.
  • each gas pressure can serve as the target gas pressure which is present in the vessel in the first 10 seconds from the end time of the liquid intake process.
  • a pressure sensor arrangement is understood to mean a device for measuring the pressure with at least one pressure sensor.
  • the vessel may comprise a piston-cylinder arrangement and a pipetting tip arranged thereon, the pressure sensor arrangement then being provided on the piston-cylinder arrangement for cost reasons. Otherwise, each pipetting tip would have to be provided with a pressure sensor arrangement, and the respective pressure sensor arrangement should be coupled to the control device after the pipetting tip has been received. This represents a considerable effort.
  • the gas pressure varying device is a mechanical device having a drive and a driven by the component which forms part of the vessel wall, so that movement of the component to an increase or decrease of the gas volume in the vessel and thus to a pressure drop or Pressure increase of the gas pressure in the vessel leads.
  • a direction of change of the gas pressure i. rising or falling, assigned a direction of movement of the component.
  • a movement play must be overcome.
  • the mentioned play of motion can be the cause of inaccuracies in the amount of fluid taken or delivered, such as when the amount of fluid dispensed or received is calculated from the movement of the drive or other sensed quantities related to the drive. Because of the play of motion, namely, there are driving activities that actually do not change the gas pressure and therefore do not change the one present in the vessel Effect amount of liquid.
  • control device is designed to determine the movement play that they follow the drive in a first drive direction in the opposite second drive direction drives until the state quantity detection device detects a change in the at least one state variable.
  • control device may be designed to control the drive in the determination of the movement play step by step. This makes it possible to decay dynamic effects before detection of the gas pressure inside the vessel.
  • each liquid metering device can individually determine its system-inherent movement play.
  • the posstechniksdosiervorraum comprises a memory device, so that the individually determined movement play can be stored therein and retrieved when needed. If the liquid metering device is intended for use under changing environmental conditions, movement games can be determined together with further variables, so that movement games determined in the storage device are stored as a function of further variables. Thus, the movement play depending on different ambient temperatures and / or ambient pressures and / or operating periods and / or component positions, etc. may be stored.
  • the aforementioned drive-movable component may be a movable piston forming a vessel wall section. However, it may also be a wall of a bellows connected to the vessel.
  • the gas pressure can advantageously be regulated in a time period which comprises a time range in the first half, preferably in the first quarter of the period lying between the end time of the fluid intake operation and the start time of the fluid delivery operation. The greatest possible security is obtained when the detection step and the control step are carried out during the entire period of time between said points in time.
  • the control step in a specific embodiment advantageously comprises driving the drive in dependence from the detected state variable.
  • a movement described above can be determined with the aid of the method according to the invention by following the drive in the opposite second drive direction following a first drive direction until the state variable detection device detects a change in the at least one state variable.
  • the driving of the drive in the second drive direction can take place step by step, wherein each activation step is associated with a detection of the at least one state variable, preferably the detection of the at least one state variable after the activation of the drive ,
  • the highest possible accuracy in the determination of the movement play can be achieved by detecting further variables during the determination of the movement play, such as the position of the component relative to the vessel or / and a temperature, in particular Ambient temperature or / and the ambient pressure.
  • the at least one determined movement play is stored, possibly together with the previously mentioned further variables assigned to the respective movement play to be stored. If necessary, then the movement play, possibly depending on currently available operating parameters, retrieved from the memory and taken into account in the control of the drive.
  • a liquid metering device according to the invention is designated generally by 10.
  • the liquid metering device 10 comprises a piston-cylinder system 12 with a piston 14, which is movably guided in a cylinder 16 in the direction of the double arrow K.
  • a replaceable pipette tip 18 is received, in which a liquid 20 is present.
  • the pipette tip 18 forms, together with the cylinder 16 and the piston 14, a vessel 20 receiving the liquid.
  • the pipette tip 18 has an opening 22, through which the liquid 20 has been received in the pipetting tip 20 and can be discharged therefrom.
  • the piston 14 is substantially gas-tight against the inner wall 16 a of the cylinder 16.
  • the piston surface 14a facing the pipette tip 18 forms a vessel boundary wall.
  • a gas 24, such as air is enclosed.
  • air it is also possible to use any other gas, for example nitrogen or a noble gas, if reactions with the liquid 20 to be taken up are to be avoided in any case.
  • the liquid 20 was introduced in a manner known per se by immersing the opening 22 in a liquid reservoir and moving the piston 14 with the opening immersed such that the volume of the enclosed gas 24 is increased, through the opening 22 into the pipette tip 18 sucked.
  • the pipetting tip 18 of Figure 1, as well as of Figure 2a and b, has already completed the liquid intake and is no longer immersed in the liquid supply.
  • a pressure sensor 26 Connected to the interior of the vessel is a pressure sensor 26 for detecting the gas pressure of the enclosed gas 24.
  • a pressure sensor 26 Connected to the interior of the vessel is a pressure sensor 26 for detecting the gas pressure of the enclosed gas 24.
  • the pressure sensor 26 detects the pressure of the gas 24 and supplies a signal representing the gas pressure via the line 28 to a control device 30, which is adapted to a drive 32 for displacement of the piston 14 in the direction of the double arrow K in response to one of the pressure sensor 26th operated signal to operate.
  • the pressure sensor 26 may be an absolute value of the pressure of the gas 24 or may provide a relative value, approximately based on the ambient pressure, to the control device 30.
  • the pressure value detected by the pressure sensor 26 and delivered to the control device 30 is indicated by a pointer 34.
  • FIG. 2 a indicates how liquid particles V evaporate from the surface 20 a into the space occupied by the gas 24.
  • the liquid 20 releases heat W to the gas 24.
  • the pressure of the gas 24 in the vessel 16 of cylinder 16, piston 14 and pipette tip 18 increases. This pressure increase is detected by the pressure sensor 26, as indicated by the changed position of the pointer 34 with respect to FIG. Without control intervention, this pressure increase would lead to a discharge of liquid 20 from the opening 22.
  • the control device 30 moves depending on the pressure sensor via the line 28 delivered pressure value to the piston in the direction of arrow 36 in Figure 2b and increases the volume of the gas 24 in the vessel from the elements 14, 16, 18 to a predetermined, explained below, target gas pressure is reached. This reduces the pressure increase due to evaporation and heat transfer.
  • the pressure of the gas 24 again reaches the value which has prevailed immediately after the reception of the liquid 20 in the pipette tip 18 in the interior of the vessel from the piston 14, cylinder 16 and pipette tip 18. The original location where the piston wall 14a was before correction is indicated at 14a '.
  • gas pressure As desired gas pressure, the pressure prevailing in the vessel at the time of the end of the liquid intake process is ideally used. Since the increase in pressure due to evaporation and / or heat transfer is usually not instantaneous, it is generally possible to use a gas pressure as target gas pressure which prevails in the vessel within a period of 10 seconds after the end of the liquid intake process.
  • the piston may also be displaced toward the opening 22 to increase the pressure of the gas 24, such as after the intake of particularly cold liquids which remove heat from the enclosed gas 24 thereby reducing its pressure.
  • FIGS. 3a and 3b Signal curves are shown in FIGS. 3a and 3b, which the pressure sensor 26 can deliver via the data line 28 to the control device 30 in determining a mechanical clearance of the drive 32 and the piston 14.
  • the relative pressure of the gas 24 is plotted against the relative position of the drive 32 upon movement of the piston 14 in the direction of the double arrow K.
  • the absolute pressure of the gas 24 may be plotted over an absolute position of the actuator 32.
  • the relative pressure may be related to the ambient pressure associated with another sensor is detected.
  • the relative position may be related to any position of the piston, such as an upper or lower dead center position.
  • each representation of Figures 3a and 3b marks the point of reversal of the direction of travel of the piston.
  • the piston is moved over the distance U to the opening 22 of the pipette tip 18 until, at the relative position U 0, an increase in the relative pressure of the gas 24 of the vessel immersed in a liquid or otherwise closed can be detected.
  • a movement of the drive 32 causes a movement of the piston 14 and thus a pressure increase of the gas 24 from the time when the drive has overcome the Stecke U upon movement in the ejection direction after the suction movement of the piston.
  • FIG. 3b the determination of the clearance during an intake movement, ie when the piston 14 is raised, is shown away from the opening 22 of the pipetting tip 18.
  • the drive 32 first has to overcome the play distance H until at a point H 0 the drive movement actually also leads to a piston movement, so that after the play distance H has been exceeded a further actuation of the drive is required a decrease in the pressure of the gas 24 in the submerged or otherwise closed vessel leads.
  • the play paths U and H that can be determined individually for each dosing device 10 can be stored in the memory 34 of the control device 30.
  • the accuracy of the drive control can be further increased by the fact that movement games are determined depending on other variables and stored in memory 34 retrievable. For example, the movement games depending on the direction and / or piston position dependent and / or temperature-dependent and / or pressure-dependent, etc. may be stored in the memory 34.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Loading And Unloading Of Fuel Tanks Or Ships (AREA)
  • Filling Or Discharging Of Gas Storage Vessels (AREA)
  • Sink And Installation For Waste Water (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)

Claims (18)

  1. Dispositif de dosage de liquide, en particulier dispositif de pipetage, destiné à aspirer et distribuer des liquides (20), le dispositif comprenant :
    - un récipient (14, 16, 18) rempli au moins en partie avec un gaz (24) et présentant une ouverture (22) à travers de laquelle un liquide (20) est prélevé dans le récipient (14, 16, 18) ou délivré à partir de celui-ci, la quantité (24) du gaz, une fois le liquide (20) prélevé, étant enfermée par des parois de récipient (14a, 16a, 18b) et le liquide (20) lui-même,
    - un dispositif de variation de pression de gaz (14, 32) pour faire varier la pression de gaz dans le récipient (14, 16, 18),
    - un dispositif de détection de paramètres d'état (26) pour détecter au moins un paramètre d'état du gaz (24) dans le récipient (14, 16, 18), et
    - un dispositif de commande (30) qui pilote le dispositif de variation de pression de gaz (14, 32) en fonction du paramètre d'état détecté par le dispositif de détection de paramètres d'état (26),
    caractérisé en ce que le dispositif de commande (30) est un dispositif de régulation (30) qui est réalisé pour piloter le dispositif de variation de pression de gaz (14, 32) au moins pendant un intervalle de temps de régulation entre le prélèvement de liquide et la distribution de liquide en fonction du paramètre d'état détecté, de telle sorte que la pression de gaz réelle dans le récipient (14, 16, 18) est maintenue pendant l'intervalle de temps de régulation sensiblement à une pression de gaz nominale prédéterminée.
  2. Dispositif de dosage de liquide selon la revendication 1, caractérisé en ce que l'intervalle de temps de régulation comprend une plage de temps dans la première moitié, de préférence dans le premier quart, de la période de temps située entre l'instant final de l'opération de prélèvement de liquide et l'instant initial de l'opération de distribution de liquide.
  3. Dispositif de dosage de liquide selon la revendication 1 ou 2, caractérisé en ce que l'intervalle de temps de régulation comprend le premier quart, de préférence la première moitié de la période de temps située entre l'instant final de l'opération de prélèvement de liquide et l'instant initial de l'opération de distribution de liquide, de préférence toute la période de temps située entre ces instants.
  4. Dispositif de dosage de liquide selon l'une quelconque des revendications précédentes, caractérisé en ce que la pression de gaz nominale prédéterminée est inférieure ou égale à une pression de gaz régnant dans le récipient (14, 16, 18) à l'instant final, ou à un instant proche de celui-ci, de l'opération de prélèvement de liquide.
  5. Dispositif de dosage de liquide selon l'une quelconque des revendications précédentes, caractérisé en ce que le dispositif de détection de paramètres d'état (26) est un ensemble de capteurs de pression (26).
  6. Dispositif de dosage de liquide selon l'une quelconque des revendications précédentes, dans lequel le dispositif de variation de pression de gaz (14, 32) est un dispositif mécanique avec un entraînement (32) et un composant (14) entraîné par celui-ci et qui forme une partie (14a) de la paroi de récipient (14a, 16a, 18b), de telle sorte qu'une variation de la pression de gaz est obtenue par le déplacement du composant (14), une direction de variation de la pression de gaz étant affectée à une direction de déplacement du composant (14), et un jeu de déplacement étant à surmonter en cas d'inversion de la direction de déplacement du composant (14),
    caractérisé en ce que le dispositif de régulation (30) est réalisé pour la détermination du jeu de déplacement de telle sorte qu'il entraîne l'entraînement (32) suite à une première direction d'entraînement dans une deuxième direction d'entraînement opposée jusqu'à ce que le dispositif de détection de paramètres d'état (26) détecte une variation dudit au moins un paramètre d'état.
  7. Dispositif de dosage de liquide selon la revendication 6, caractérisé en ce que le dispositif de régulation (30) est réalisé pour piloter l'entraînement (32) pas à pas afin de déterminer le jeu de déplacement.
  8. Dispositif de dosage de liquide selon la revendication 6 ou 7, caractérisé en ce qu'il comprend un dispositif de mémoire (34) qui est réalisé pour mémoriser le jeu de déplacement déterminé, de préférence en conjonction avec d'autres paramètres associés au jeu de déplacement respectivement déterminé.
  9. Dispositif de dosage de liquide selon la revendication 8, caractérisé en ce qu'il est réalisé pour détecter la position du composant (14), le dispositif de mémoire (34) étant réalisé pour mémoriser des quantités de valeur composées du jeu de déplacement déterminé et d'une position de composant respectivement affectée à celui-ci.
  10. Dispositif de dosage de liquide selon l'une quelconque des revendications précédentes, caractérisé en ce que le composant (14) est un piston mobile (14) formant une section de paroi de récipient (14a) et/ou en ce que le récipient (14, 16, 18) comprend une pointe de pipetage (18) de préférence échangeable.
  11. Procédé permettant d'éviter des pertes de goutte sur des dispositifs de dosage de liquide (10), en particulier des dispositifs de pipetage (10), comprenant les étapes suivantes qui sont exécutées au moins dans un intervalle de temps entre l'opération de prélèvement de liquide et l'opération de distribution de liquide :
    - détecter au moins un paramètre d'état d'un gaz (24) qui est substantiellement enfermé dans un récipient (14, 16, 18) prélevant un liquide (20) du dispositif de dosage de liquide (14, 16, 18) entre des parois de récipient (14a, 16a, 18b) et le liquide (20),
    - réguler la pression du gaz (24) en fonction du paramètre d'état détecté de telle sorte que la pression de gaz réelle coïncide substantiellement avec une pression de gaz nominale prédéterminée.
  12. Procédé selon la revendication 11, caractérisé en ce que l'étape de détection et l'étape de régulation ont lieu pendant un intervalle de temps de régulation qui comprend une plage de temps dans la première moitié, de préférence dans le premier quart, de la période de temps située entre l'instant final de l'opération de prélèvement de liquide et l'instant initial de l'opération de distribution de liquide, de préférence toute la période de temps située entre ces instants.
  13. Procédé selon la revendication 11 ou 12, dans lequel le dispositif de dosage de liquide comprend un dispositif mécanique avec un entraînement et un composant (14) entraîné par celui-ci et formant une section de paroi de récipient (14a) en tant que moyens (14, 30, 32) pour la variation de pression de gaz, de telle sorte qu'une variation de la pression de gaz est obtenue par le déplacement du composant (14),
    caractérisé en ce que l'étape de régulation comprend un pilotage de l'entraînement (32) en fonction du paramètre d'état détecté.
  14. Procédé selon la revendication 13, dans lequel une direction de déplacement du composant (14) est affectée à une direction de variation de la pression de gaz, et dans lequel un jeu de déplacement doit être surmonté en cas d'inversion de la direction de déplacement du composant (14),
    caractérisé en ce que pour la détermination du jeu de déplacement, suite à une première direction d'entraînement, l'entraînement (32) est piloté dans une deuxième direction d'entraînement opposée jusqu'à ce que le dispositif de détection de paramètres d'état (26) détecte une variation dudit au moins un paramètre d'état.
  15. Procédé selon la revendication 14, caractérisé en ce que le pilotage de l'entraînement (32) a lieu pas à pas dans la deuxième direction d'entraînement, une détection dudit au moins un paramètre d'état étant affectée à chaque étape de pilotage.
  16. Procédé selon la revendication 14 ou 15, caractérisé en ce que pendant la détermination du jeu de déplacement, d'autres paramètres sont détectés, comme par exemple la position du composant (14) par rapport au récipient (14, 16, 18) ou/et une température, en particulier une température ambiante.
  17. Procédé selon l'une quelconque des revendications 14 à 16, caractérisé en ce qu'il comprend une étape d'une mémorisation du jeu de déplacement déterminé, éventuellement en conjonction avec d'autres paramètres affectés au jeu de déplacement.
  18. Procédé selon l'une quelconque des revendications 11 à 16, caractérisé en ce que le paramètre d'état détecté est la pression de gaz du gaz enfermé (24).
EP04015682A 2004-07-02 2004-07-02 Pipette évitant la formation de goutte et son mode de fonctionnement Active EP1614468B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP04015682A EP1614468B1 (fr) 2004-07-02 2004-07-02 Pipette évitant la formation de goutte et son mode de fonctionnement
ES04015682T ES2274349T3 (es) 2004-07-02 2004-07-02 Dispositivo pipeteado seguro contra goteo y procedimiento de pipeteado seguro contra goteo.
AT04015682T ATE348659T1 (de) 2004-07-02 2004-07-02 Abtropfsichere pipettiervorrichtung und abtropfsicheres pipettierverfahren
DE502004002377T DE502004002377D1 (de) 2004-07-02 2004-07-02 Abtropfsichere Pipettiervorrichtung und abtropfsicheres Pipettierverfahren

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP04015682A EP1614468B1 (fr) 2004-07-02 2004-07-02 Pipette évitant la formation de goutte et son mode de fonctionnement

Publications (2)

Publication Number Publication Date
EP1614468A1 EP1614468A1 (fr) 2006-01-11
EP1614468B1 true EP1614468B1 (fr) 2006-12-20

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EP04015682A Active EP1614468B1 (fr) 2004-07-02 2004-07-02 Pipette évitant la formation de goutte et son mode de fonctionnement

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AT (1) ATE348659T1 (fr)
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Publication number Priority date Publication date Assignee Title
US8096197B2 (en) 2006-03-31 2012-01-17 Artel, Inc. Air displacement liquid delivery system and related method
ATE526085T1 (de) * 2007-06-15 2011-10-15 Hamilton Bonaduz Ag Probenbehandlungsanordnung für eine flüssigkeitsdosiervorrichtung
US20110318242A1 (en) * 2008-11-28 2011-12-29 Hamilton Bonaduz Ag Metering device suited for metering very small metering volumes and metering method
US8231842B2 (en) 2010-01-22 2012-07-31 Tecan Trading Ag Positive displacement pump with pressure sensor
US20130045498A1 (en) 2010-03-01 2013-02-21 Novozymes A/S Viscosity pressure assay
DE102011081186A1 (de) * 2011-08-18 2013-02-21 Hamilton Bonaduz Ag Verfahren zum Detektieren der Oberfläche einer Flüssigkeitsprobe in einem Probenbehälter
DE102018125196A1 (de) * 2018-10-11 2020-04-16 Hamilton Bonaduz Ag Vorrichtung und Verfahren zur Qualitätsbestimmung einer impulsartigen Flüssigkeitsdispensation nach dem Air-Displacement-Prinzip
US11320295B2 (en) * 2019-04-26 2022-05-03 Festo Se & Co. Kg Dosing unit and method for dosing a liquid
DE102019126731A1 (de) * 2019-10-02 2021-04-08 Hamilton Bonaduz Ag Vorrichtung und Verfahren zum exakten flüssigkeitsklassen-unabhängigen Pipettieren
EP3832315A1 (fr) * 2019-12-06 2021-06-09 F. Hoffmann-La Roche AG Unité de pipette et procédé de pipetage pour récipients de liquide fermés

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DE3531241A1 (de) * 1985-08-31 1987-03-05 Eppendorf Geraetebau Netheler Vorrichtung zur gesteuerten ausgabe von fluessigkeiten
JP3318629B2 (ja) * 1993-06-18 2002-08-26 ソニー株式会社 液体の吸引／排出装置及び方法
US5503036A (en) * 1994-05-09 1996-04-02 Ciba Corning Diagnostics Corp. Obstruction detection circuit for sample probe
FI101864B1 (fi) * 1995-07-07 1998-09-15 Biohit Oy Menetelmä nesteannosteluvirheen korjaamiseksi, ja nesteannostelulaite
DE10118875C1 (de) * 2001-04-18 2002-09-12 Eppendorf Ag Verfahren zum kontrollierten Dosieren von Flüssigkeiten unter Verdrängung eines Gaspolsters

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DE502004002377D1 (de) 2007-02-01
ES2274349T3 (es) 2007-05-16
ATE348659T1 (de) 2007-01-15
EP1614468A1 (fr) 2006-01-11

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