EP1463821A4 - INCREASED GENTRANSFEREFFICIENCY BY PRE-INCUBATION WITH ENDOTHELIAL CELLS - Google Patents

INCREASED GENTRANSFEREFFICIENCY BY PRE-INCUBATION WITH ENDOTHELIAL CELLS

Info

Publication number
EP1463821A4
EP1463821A4 EP02795952A EP02795952A EP1463821A4 EP 1463821 A4 EP1463821 A4 EP 1463821A4 EP 02795952 A EP02795952 A EP 02795952A EP 02795952 A EP02795952 A EP 02795952A EP 1463821 A4 EP1463821 A4 EP 1463821A4
Authority
EP
European Patent Office
Prior art keywords
incubation
efficacy
increasing
endothelial cells
gene transfer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP02795952A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP1463821A1 (en
Inventor
John P Chute
Abha A Saini
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Navy
US Department of Health and Human Services
Original Assignee
US Department of Navy
US Department of Health and Human Services
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by US Department of Navy, US Department of Health and Human Services filed Critical US Department of Navy
Publication of EP1463821A1 publication Critical patent/EP1463821A1/en
Publication of EP1463821A4 publication Critical patent/EP1463821A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
EP02795952A 2001-12-21 2002-12-20 INCREASED GENTRANSFEREFFICIENCY BY PRE-INCUBATION WITH ENDOTHELIAL CELLS Withdrawn EP1463821A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US34186801P 2001-12-21 2001-12-21
US341868P 2001-12-21
PCT/US2002/040719 WO2003060138A1 (en) 2001-12-21 2002-12-20 In increasing gene transfer efficiency by pre-incubation with endothelial cells

Publications (2)

Publication Number Publication Date
EP1463821A1 EP1463821A1 (en) 2004-10-06
EP1463821A4 true EP1463821A4 (en) 2006-05-31

Family

ID=23339354

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02795952A Withdrawn EP1463821A4 (en) 2001-12-21 2002-12-20 INCREASED GENTRANSFEREFFICIENCY BY PRE-INCUBATION WITH ENDOTHELIAL CELLS

Country Status (4)

Country Link
EP (1) EP1463821A4 (https=)
JP (1) JP2005514071A (https=)
AU (1) AU2002360674A1 (https=)
WO (1) WO2003060138A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2605080A1 (en) * 2005-04-21 2006-11-02 Massachusetts Institute Of Technology Materials and methods for altering an immune response to exogenous and endogenous immunogens, including syngeneic and non-syngeneic cells, tissues or organs
CN101735979B (zh) * 2009-12-31 2014-04-30 浙江中赢控股集团有限公司 体外扩增造血干细胞和前体细胞的方法
EP3060670B1 (en) * 2013-10-24 2019-07-10 Ospedale San Raffaele S.r.l. Method

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5811297A (en) * 1996-03-07 1998-09-22 Amba Biosciences, Llc Immortalized hematopoietic cell lines, cell system thereof with stromal cells, in vitro, ex vivo and in vivo uses, & in vitro generation of dendritic cells and macrophages
WO2001094551A2 (de) * 2000-06-09 2001-12-13 Universitätsklinikum Charite Medizinische Fakultät Der Humboldt-Universität Zu Berlin, Akademische Verwaltung - Forschung Gen-modifizierte t-zellen, verfahren zu ihrer herstellung und ihre verwendung
WO2002078449A2 (en) * 2001-04-02 2002-10-10 Advanced Cell Technology, Inc. Method for facilitating the production of differentiated cell types and tissues from embryonic and adult pluripotent and multipotent cells

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHU P ET AL: "RETROVIRUS-MEDIATED GENE TRANSFER INTO HUMAN HEMATOPOIETIC STEM CELLS", JOURNAL OF MOLECULAR MEDICINE, SPRINGER VERLAG, DE, vol. 76, 1998, pages 184 - 192, XP002939375, ISSN: 0946-2716 *
CHUTE J P ET AL: "A COMPARATIVE STUDY OF THE CELL CYCLE STATUS AND PRIMITIVE CELL ADHESION MOLECULE PROFILE OF HUMAN CD34+CELLS CULTURED IN STROMA-FREE VERSUS PORCINE MICROVASCULAR ENDOTHELIAL CELL CULTURES", EXPERIMENTAL HEMATOLOGY, NEW YORK, NY, US, vol. 27, 1999, pages 370 - 379, XP000929912, ISSN: 0301-472X *
CHUTE J P ET AL: "Preincubation with endothelial cell monolayers increases gene transfer efficiency into human bone marrow CD34+CD38-progenitor cells", HUMAN GENE THERAPY, vol. 11, 10 December 2000 (2000-12-10), pages 2515 - 2528, XP002963382, ISSN: 1043-0342 *
ROSLER E ET AL: "Cocultivation of umbilical cord blood cells with endothelial cells leads to extensive amplification of competent CD34+CD38-cells", EXPERIMENTAL HEMATOLOGY, NEW YORK, NY, US, vol. 28, 2000, pages 841 - 852, XP002963383, ISSN: 0301-472X *
See also references of WO03060138A1 *
ZIEGLER B L ET AL: "EXPANSION OF STEM AND PROGENITOR CELLS", CURRENT OPINION IN HEMATOLOGY, RAPID SCIENCE PUBLISHERS, PHILADELPHIA, PA, US, vol. 5, no. 6, November 1998 (1998-11-01), pages 434 - 440, XP001009708, ISSN: 1065-6251 *

Also Published As

Publication number Publication date
AU2002360674A1 (en) 2003-07-30
JP2005514071A (ja) 2005-05-19
WO2003060138A1 (en) 2003-07-24
EP1463821A1 (en) 2004-10-06

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Legal Events

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Inventor name: SAINI, ABHA, A.

Inventor name: CHUTE, JOHN, P.

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