EP1451367A4 - Genie de peptides leader pour la secretion de proteines recombinees dans des bacteries - Google Patents

Genie de peptides leader pour la secretion de proteines recombinees dans des bacteries

Info

Publication number
EP1451367A4
EP1451367A4 EP02795597A EP02795597A EP1451367A4 EP 1451367 A4 EP1451367 A4 EP 1451367A4 EP 02795597 A EP02795597 A EP 02795597A EP 02795597 A EP02795597 A EP 02795597A EP 1451367 A4 EP1451367 A4 EP 1451367A4
Authority
EP
European Patent Office
Prior art keywords
secretion
bacteria
engineering
recombinant proteins
leader peptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP02795597A
Other languages
German (de)
English (en)
Other versions
EP1451367A2 (fr
Inventor
George Georgiou
Matthew Delisa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Research Development Foundation
Original Assignee
Research Development Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Research Development Foundation filed Critical Research Development Foundation
Publication of EP1451367A2 publication Critical patent/EP1451367A2/fr
Publication of EP1451367A4 publication Critical patent/EP1451367A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
EP02795597A 2001-11-05 2002-11-05 Genie de peptides leader pour la secretion de proteines recombinees dans des bacteries Withdrawn EP1451367A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US33745201P 2001-11-05 2001-11-05
US337452P 2001-11-05
PCT/US2002/035618 WO2003040335A2 (fr) 2001-11-05 2002-11-05 Genie de peptides leader pour la secretion de proteines recombinees dans des bacteries

Publications (2)

Publication Number Publication Date
EP1451367A2 EP1451367A2 (fr) 2004-09-01
EP1451367A4 true EP1451367A4 (fr) 2006-06-14

Family

ID=23320598

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02795597A Withdrawn EP1451367A4 (fr) 2001-11-05 2002-11-05 Genie de peptides leader pour la secretion de proteines recombinees dans des bacteries

Country Status (3)

Country Link
EP (1) EP1451367A4 (fr)
CN (1) CN100564540C (fr)
WO (1) WO2003040335A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2548030B1 (fr) * 2010-03-18 2015-05-27 Cornell University Préparation d'anticorps correctement pliés utilisant la présentation sur la membrane intérieure d'intermédiaires de translocation twin-arginine
KR20140004219A (ko) * 2011-04-08 2014-01-10 앤섬 바이오사이언시즈 프라이빗 리미티드 대장균에서 이형 단백질 생산을 위한 새로운 발현 및 분비 벡터 시스템
KR101470595B1 (ko) 2012-08-01 2014-12-10 대구가톨릭대학교산학협력단 세포외로 분비되는 녹색형광단백질, 이를 코딩하는 유전자 및 이 유전자 발현을 위한 벡터
CN102851270A (zh) * 2012-08-03 2013-01-02 江南大学 一种杂交链霉菌胰蛋白酶酶原及其应用
GB201713732D0 (en) * 2017-08-25 2017-10-11 Alta Innovations Ltd Tat expression system
CN109575116B (zh) * 2018-11-09 2022-04-22 广东海洋大学 一种线粒体定位导肽及其发现方法与应用
CN110616227A (zh) * 2019-09-30 2019-12-27 天津科技大学 一种来源于黄粉虫的抗冻蛋白的基因、重组表达载体、工程菌株和应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999045136A1 (fr) * 1998-03-05 1999-09-10 University Of British Columbia Procedes d'analyse d'inhibiteurs de secretion de type iii
WO1999051753A1 (fr) * 1998-04-01 1999-10-14 The Governors Of The University Of Alberta Compositions et methodes de secretion proteique
WO2002022667A2 (fr) * 2000-09-18 2002-03-21 Genencor International, Inc. Translocation des arginines couplees dans bacillus
WO2002055717A2 (fr) * 2000-10-10 2002-07-18 Genencor Int Secretion amelioree d'un polypeptide par un micro-organisme

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999045136A1 (fr) * 1998-03-05 1999-09-10 University Of British Columbia Procedes d'analyse d'inhibiteurs de secretion de type iii
WO1999051753A1 (fr) * 1998-04-01 1999-10-14 The Governors Of The University Of Alberta Compositions et methodes de secretion proteique
WO2002022667A2 (fr) * 2000-09-18 2002-03-21 Genencor International, Inc. Translocation des arginines couplees dans bacillus
WO2002055717A2 (fr) * 2000-10-10 2002-07-18 Genencor Int Secretion amelioree d'un polypeptide par un micro-organisme

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
ANDERSEN ET AL: "New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 64, no. 6, June 1998 (1998-06-01), pages 2240 - 2246, XP002203612, ISSN: 0099-2240 *
BERG B L ET AL: "NITRATE-INDUCIBLE FORMATE DEHYDROGENASE IN ESCHERICHIA-COLI K-12 I. NUCLEOTIDE SEQUENCE OF THE FDN GHI OPERON AND EVIDENCE THAT OPAL UGA ENCODES SELENOCYSTEINE", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 266, no. 33, 1991, pages 22380 - 22385, XP002330126, ISSN: 0021-9258 *
BLAUDECK N ET AL: "Specificity of signal peptide recognition in tat-dependent bacterial protein translocation", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 183, no. 2, January 2001 (2001-01-01), pages 604 - 610, XP002989193, ISSN: 0021-9193 *
BUCHANAN G ET AL: "A genetic screen for suppressors of escherichia coli tat signal peptide mutations establishes a critical role for the second arginine within the twin-arginine motif", ARCHIVES OF MICROBIOLOGY, BERLIN, DE, vol. 177, 30 October 2001 (2001-10-30), pages 107 - 112, XP002971648, ISSN: 0302-8933 *
DELISA MATTHEW P ET AL: "Phage shock protein PspA of Escherichia coli relieves saturation of protein export via the Tat pathway.", JOURNAL OF BACTERIOLOGY, vol. 186, no. 2, January 2004 (2004-01-01), pages 366 - 373, XP002378230, ISSN: 0021-9193 *
FEILMEIER BRADLEY J ET AL: "Green fluorescent protein functions as a reporter for protein localization in Escherichia coli", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 182, no. 14, July 2000 (2000-07-01), pages 4068 - 4076, XP002263489, ISSN: 0021-9193 *
HINSLEY^A A P ET AL: "A naturally occurring bacterial Tat signal peptide lacking one of the 'invariant' arginine residues of the consensus targeting motif", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 497, no. 1, 18 May 2001 (2001-05-18), pages 45 - 49, XP004250034, ISSN: 0014-5793 *
IZE B ET AL: "In vivo dissection of the Tat translocation pathway in Escherichia coli", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 317, no. 3, 29 March 2002 (2002-03-29), pages 327 - 335, XP004468971, ISSN: 0022-2836 *
KLEIN B K ET AL: "EFFECTS OF SIGNAL PEPTIDE CHANGES ON THE SECRETION OF BOVINE SOMATOTROPIN (BST) FROM ESCHERICHIA COLI", PROTEIN ENGINEERING, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 5, no. 6, September 1992 (1992-09-01), pages 511 - 517, XP009006075, ISSN: 0269-2139 *
MATTEUCCI M ET AL: "ALKALINE PHOSPHATASE FUSIONS: A TAG TO IDENTIFY MUTATIONS THAT RESULT IN INCREASED EXPRESSION OF SECRETED HUMAN GROWTH HORMONE FROM E. COLI", BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, vol. 4, January 1986 (1986-01-01), pages 51 - 55, XP002006627, ISSN: 0733-222X *
SANTINI C-L ET AL: "Translocation of jellyfish green fluorescent protein via the Tat system of Escherichia coli and change of its periplasmic localization in response to osmotic up-shock", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 276, no. 11, 16 March 2001 (2001-03-16), pages 8159 - 8164, XP002273739, ISSN: 0021-9258 *
THOMAS JOANNA D ET AL: "Export of active green fluorescent protein to the periplasm by the twin-arginine translocase (Tat) pathway in Escherichia coli", MOLECULAR MICROBIOLOGY, vol. 39, no. 1, January 2001 (2001-01-01), pages 47 - 53, XP002330124, ISSN: 0950-382X *

Also Published As

Publication number Publication date
WO2003040335A3 (fr) 2003-12-31
EP1451367A2 (fr) 2004-09-01
CN100564540C (zh) 2009-12-02
WO2003040335A2 (fr) 2003-05-15
CN1788092A (zh) 2006-06-14

Similar Documents

Publication Publication Date Title
IL158751A0 (en) Recombinant fusion proteins and the trimers thereof
EP1379132A4 (fr) Proteines secretees humaines
IL159894A0 (en) Therapeutic agents comprising pro-apoptotic proteins
HK1071354A1 (en) ß-AMYLOID PROTEIN PRODUCTION/SECRETION INHIBITORS
EP1392817A4 (fr) Proteines secretees par l'etre humain
HUP0402496A3 (en) Growth hormone fusion protein
AU2002361452A8 (en) Secreted proteins
IL162598A0 (en) Secreted protein
IL138946A0 (en) Method for the identification of peptides and proteins
EP1451367A4 (fr) Genie de peptides leader pour la secretion de proteines recombinees dans des bacteries
EP1441030A4 (fr) Procede de purification de proteine fusionnee recombinee et procede de production de proteine utilisant ledit procede de purification
AU2002354803A8 (en) Secreted proteins
EP1368468A4 (fr) 83 proteines humaines secretes
EP1385977A4 (fr) Proteines secretees
AU2001281464A1 (en) Recombinant proteins containing repeating units
EP1370651A4 (fr) 70 proteines humaines secretees
EP1417224A4 (fr) Proteines secretees
EP1458736A4 (fr) Thrombine bovine de recombinaison
AU5603201A (en) Co-expression of recombinant proteins
EP1385979A4 (fr) Procedes pour renforcer la traduction et l'expression de proteines recombinees
AU2002245311A8 (en) Human protein kinase n-like polypeptide and uses thereof
GB0101739D0 (en) Novel polypeptide
AU2002348034A8 (en) 16 human secreted proteins
IL156068A0 (en) Method for improving homogeneity and/or secretion of a recombinant protein
GB0101469D0 (en) Polypeptide Delivery

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20040507

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR

AX Request for extension of the european patent

Extension state: AL LT LV MK RO SI

A4 Supplementary search report drawn up and despatched

Effective date: 20060512

17Q First examination report despatched

Effective date: 20060915

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20100511