EP1438567B1 - Procedes et systemes de traitement microfluidique - Google Patents

Procedes et systemes de traitement microfluidique Download PDF

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Publication number
EP1438567B1
EP1438567B1 EP02715213.1A EP02715213A EP1438567B1 EP 1438567 B1 EP1438567 B1 EP 1438567B1 EP 02715213 A EP02715213 A EP 02715213A EP 1438567 B1 EP1438567 B1 EP 1438567B1
Authority
EP
European Patent Office
Prior art keywords
microfluidic
sample
lysing
zone
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP02715213.1A
Other languages
German (de)
English (en)
Other versions
EP1438567A1 (fr
EP1438567A4 (fr
Inventor
Gene Parunak
Kalyan Handique
Betty Wu
Karthik Ganesan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HandyLab Inc
Original Assignee
HandyLab Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/014,519 external-priority patent/US7192557B2/en
Priority claimed from US10/014,520 external-priority patent/US7270786B2/en
Priority claimed from US10/075,371 external-priority patent/US7323140B2/en
Application filed by HandyLab Inc filed Critical HandyLab Inc
Priority to EP18185265.8A priority Critical patent/EP3427834A1/fr
Publication of EP1438567A1 publication Critical patent/EP1438567A1/fr
Publication of EP1438567A4 publication Critical patent/EP1438567A4/fr
Application granted granted Critical
Publication of EP1438567B1 publication Critical patent/EP1438567B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet

Definitions

  • Another aspect of the present disclosure relates to a microfluidic system and method for processing a particle-containing fluid, such as, for example, a liquid containing bacterial cells or human cells.
  • a particle-containing fluid such as, for example, a liquid containing bacterial cells or human cells.
  • the system includes a lysing zone to receive the cell-containing sample and a positioning element to position the cell-containing sample in a lysing position in the vicinity of a lysing mechanism.
  • the lysing mechanism releases intracellular material, such as DNA or RNA, from the cells.
  • the lysing mechanism includes electrodes for generating an electric field sufficient to release intracellular contents from the cells.
  • the lysing mechanism may lyse the cells using chemical, heat and/or ultrasonic techniques or any combination of these techniques.
  • actuator 170 of the microdroplet preparation module 158 drives a microdroplet into cell lysis module 160.
  • vented positioning element 200 positions microdroplet 802 in a lysing position with respect to electrodes 954. More specifically, as the microdroplet arrives in lysing module 160 it passes the opening of positioning element 200, because second positioning element 206 inhibits the microdroplet from flowing into vent 202.
  • the propulsion gas from actuator 170 dissipates through vent 202, thereby substantially equalizing gas pressure upstream of microdroplet 802 with a pressure downstream of microdroplet 802.
  • the microdroplet stops movement at a lysing position just downstream from barrier 200.
  • substantially all of microdroplet 802 is disposed between an upstream edge 212 and a downstream edge 214 of electrodes 954.
  • Reagent input module 152 is essentially the same as microdroplet formation module 158, however, it is specifically designed for formation of a microdroplet of reagent having a predetermined volume which will yield a desired ratio of reagent to sample when mixed with the microdroplet from cell lysing module 160.
  • Module 152 includes an input port 420, a valve 422, and an actuator 172, each of which joins a reagent source channel 428.
  • An overflow channel 424, which also joins reagents source channel 428, may also be provided.
  • Actuator 172 may include a second positioning element 432 to prevent liquid from entering therein.
  • the upstream gas dissipates through vent 508, thereby reducing the upstream pressure.
  • the pressure reduction which preferably equalizes the downstream and upstream pressures, reduces or eliminates the motive force tending to urge the microfluidic sample downstream.

Claims (9)

  1. Dispositif microfluidique (110) pour traiter un échantillon microfluidique contenant des cellules, comprenant :
    un module de lyse (300) configuré pour recevoir un échantillon microfluidique contenant des cellules, dans lequel le module de lyse comprend une zone de lyse (302), dans lequel l'échantillon microfluidique est un liquide ;
    un élément de positionnement (312) configuré pour inhiber le déplacement en aval de l'échantillon microfluidique pour positionner l'échantillon microfluidique dans une position de lyse ;
    un mécanisme de lyse (308) dans la zone de lyse (302), pour libérer un contenu intracellulaire des cellules dans la zone de lyse ; et
    un actionneur à gaz (314) disposé en amont de l'élément de positionnement (312) et de la zone de lyse (302) de telle sorte qu'une première partie de l'échantillon microfluidique soit disposée en amont de l'actionneur à gaz (314) et qu'une seconde partie de l'échantillon microfluidique soit disposée en aval de l'actionneur à gaz (314) et en amont de l'élément de positionnement (312), l'actionneur à gaz (314) étant configuré pour fournir une pression de gaz suffisante pour préparer une microgouttelette (304) d'un volume prédéterminé comprenant le contenu intracellulaire libéré des cellules de l'échantillon microfluidique contenant des cellules dans la zone de lyse (302) en séparant la seconde partie de l'échantillon microfluidique de la première partie de l'échantillon microfluidique et en déplaçant la seconde partie de l'échantillon microfluidique jusqu'à un emplacement en aval du mécanisme de lyse (308) et de l'élément de positionnement (312).
  2. Dispositif microfluidique (110) selon la revendication 1, le dispositif comprenant un substrat (130,132), et dans lequel le mécanisme de lyse (308) et l'actionneur à gaz (314) font partie intégrante du substrat.
  3. Dispositif microfluidique (110) selon la revendication 1 ou la revendication 2, dans lequel l'actionneur à gaz (314) comprend une source de chaleur pour chauffer une quantité de gaz augmentant ainsi une pression du gaz.
  4. Dispositif micro fluidique (110) selon l'une quelconque des revendications précédentes, dans lequel l'élément de positionnement (312) comprend un évent pour sensiblement égaliser une pression de gaz en amont de l'échantillon microfluidique contenant des cellules avec une pression de gaz en aval de l'échantillon microfluidique contenant des cellules quand l'échantillon microfluidique contenant des cellules se trouve dans la position de lyse pour ainsi inhiber le déplacement en aval de l'échantillon microfluidique contenant des cellules en aval de la position de lyse.
  5. Procédé microfluidique pour traiter un échantillon microfluidique contenant des cellules, comprenant :
    le positionnement, à l'aide d'un élément de positionnement (312), de l'échantillon microfluidique contenant des cellules dans une position de lyse par rapport à un mécanisme de lyse (308) d'une zone de lyse (302) d'un module de lyse (300) d'un dispositif microfluidique (110), l'échantillon microfluidique contenant des cellules comprenant un liquide contenant des cellules ;
    l'actionnement du mécanisme de lyse (308) pour libérer une substance intracellulaire des cellules de l'échantillon microfluidique contenant des cellules ;
    l'actionnement d'un actionneur à gaz (314) disposé en amont de l'élément de positionnement (312) et de la zone de lyse (302) pour fournir une pression de gaz suffisante pour séparer une partie de l'échantillon microfluidique contenant des cellules qui se trouve en aval de l'actionneur à gaz (314) et en amont de l'élément de positionnement (312) d'une partie de l'échantillon microfluidique contenant des cellules qui se trouve en amont de l'actionneur à gaz (314) et déplacer la partie aval de l'échantillon microfluidique contenant des cellules à un emplacement en aval du mécanisme de lyse (308) et de l'élément de positionnement (312), la partie aval de l'échantillon microfluidique étant une microgouttelette (304) comprenant une substance intracellulaire libérée de cellules de l'échantillon microfluidique contenant des cellules et ayant un volume prédéterminé.
  6. Procédé microfluidique selon la revendication 5, dans lequel l'étape de positionnement comprend la mise en contact de la surface aval de l'échantillon microfluidique avec une matière hydrophobe.
  7. Procédé microfluidique selon l'une quelconque des revendications 5 ou 6, dans lequel l'étape de positionnement comprend l'augmentation d'un rayon de courbure de l'échantillon microfluidique.
  8. Procédé microfluidique selon l'une quelconque des revendications 5 à 7, dans lequel l'étape de positionnement comprend l'égalisation sensible d'une pression de gaz en amont de l'échantillon microfluidique avec une pression de gaz en aval de l'échantillon microfluidique.
  9. Procédé microfluidique selon l'une quelconque des revendications 5 à 8, dans lequel la seconde partie de l'échantillon microfluidique comprend moins d'environ 90 pour cent de l'échantillon microfluidique contenant des cellules.
EP02715213.1A 2001-07-26 2002-03-27 Procedes et systemes de traitement microfluidique Expired - Lifetime EP1438567B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP18185265.8A EP3427834A1 (fr) 2001-07-26 2002-03-27 Procedes et systemes de traitement microfluidique

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US75371 1979-09-10
US14519 1998-01-28
US30763801P 2001-07-26 2001-07-26
US307638P 2001-07-26
US14520 2001-12-14
US10/014,519 US7192557B2 (en) 2001-03-28 2001-12-14 Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids
US10/014,520 US7270786B2 (en) 2001-03-28 2001-12-14 Methods and systems for processing microfluidic samples of particle containing fluids
US10/075,371 US7323140B2 (en) 2001-03-28 2002-02-15 Moving microdroplets in a microfluidic device
PCT/US2002/009440 WO2003012406A1 (fr) 2001-07-26 2002-03-27 Procedes et systemes de traitement microfluidique

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP18185265.8A Division EP3427834A1 (fr) 2001-07-26 2002-03-27 Procedes et systemes de traitement microfluidique

Publications (3)

Publication Number Publication Date
EP1438567A1 EP1438567A1 (fr) 2004-07-21
EP1438567A4 EP1438567A4 (fr) 2010-06-02
EP1438567B1 true EP1438567B1 (fr) 2018-07-25

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EP02715213.1A Expired - Lifetime EP1438567B1 (fr) 2001-07-26 2002-03-27 Procedes et systemes de traitement microfluidique
EP18185265.8A Withdrawn EP3427834A1 (fr) 2001-07-26 2002-03-27 Procedes et systemes de traitement microfluidique

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Application Number Title Priority Date Filing Date
EP18185265.8A Withdrawn EP3427834A1 (fr) 2001-07-26 2002-03-27 Procedes et systemes de traitement microfluidique

Country Status (4)

Country Link
EP (2) EP1438567B1 (fr)
JP (1) JP4596776B2 (fr)
ES (1) ES2683698T3 (fr)
WO (1) WO2003012406A1 (fr)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
WO2005011867A2 (fr) * 2003-07-31 2005-02-10 Handylab, Inc. Traitement d'echantillons contenant des particules
US7665303B2 (en) 2004-03-31 2010-02-23 Lifescan Scotland, Ltd. Method of segregating a bolus of fluid using a pneumatic actuator in a fluid handling circuit
US7059352B2 (en) 2004-03-31 2006-06-13 Lifescan Scotland Triggerable passive valve for use in controlling the flow of fluid
US7156117B2 (en) 2004-03-31 2007-01-02 Lifescan Scotland Limited Method of controlling the movement of fluid through a microfluidic circuit using an array of triggerable passive valves
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
ES2553097T3 (es) 2004-05-03 2015-12-04 Handylab, Inc. Procesamiento de muestras que contienen polinucleótidos
WO2007112114A2 (fr) 2006-03-24 2007-10-04 Handylab, Inc. Système intégré de traitement d'échantillons microfluides et son procédé d'utilisation
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
EP2091647A2 (fr) 2006-11-14 2009-08-26 Handylab, Inc. Système microfluidique utilisé pour amplifier et détecter des polynucléotides en parallèle
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8324372B2 (en) 2007-07-13 2012-12-04 Handylab, Inc. Polynucleotide capture materials, and methods of using same
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
WO2012142516A1 (fr) 2011-04-15 2012-10-18 Becton, Dickinson And Company Thermocycleur microfluidique en temps réel à balayage et procédés synchronisés de thermocyclage et de détection optique à balayage
ES2825905T3 (es) 2011-09-30 2021-05-17 Becton Dickinson Co Tira reactiva unificada
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
WO2013067202A1 (fr) 2011-11-04 2013-05-10 Handylab, Inc. Dispositif de préparation d'échantillons de polynucléotides
CN107881219B (zh) 2012-02-03 2021-09-10 贝克顿·迪金森公司 用于分子诊断测试分配和测试之间兼容性确定的外部文件
WO2020112081A1 (fr) * 2018-11-26 2020-06-04 Hewlett-Packard Development Company, L.P. Dispositifs microfluidiques
CN112023990B (zh) * 2019-06-03 2023-06-23 利多(香港)有限公司 一种微流控检测芯片及制造方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6130098A (en) * 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5304487A (en) * 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
US5639423A (en) * 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US6168948B1 (en) * 1995-06-29 2001-01-02 Affymetrix, Inc. Miniaturized genetic analysis systems and methods
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5863502A (en) * 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
JPH10108666A (ja) * 1996-10-04 1998-04-28 Asahi Medical Co Ltd 中空糸型培養器
US6379929B1 (en) * 1996-11-20 2002-04-30 The Regents Of The University Of Michigan Chip-based isothermal amplification devices and methods
EP0941766B1 (fr) * 1998-03-12 2006-12-20 Miltenyi Biotec GmbH Système à colonne micro pour séparation magnétique
JP4074713B2 (ja) * 1998-07-29 2008-04-09 財団法人川村理化学研究所 送液デバイス及びその製造方法
JP2000166535A (ja) * 1998-12-10 2000-06-20 Nippon Laser Denshi Kk 微量分注針体
DE19948473A1 (de) * 1999-10-08 2001-04-12 Nmi Univ Tuebingen Verfahren und Vorrichtung zum Messen an in einer flüssigen Umgebung befindlichen Zellen
US6272939B1 (en) * 1999-10-15 2001-08-14 Applera Corporation System and method for filling a substrate with a liquid sample

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6130098A (en) * 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets

Also Published As

Publication number Publication date
JP2005514718A (ja) 2005-05-19
ES2683698T3 (es) 2018-09-27
EP1438567A1 (fr) 2004-07-21
WO2003012406A1 (fr) 2003-02-13
WO2003012406A9 (fr) 2003-03-20
EP3427834A1 (fr) 2019-01-16
JP4596776B2 (ja) 2010-12-15
EP1438567A4 (fr) 2010-06-02

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