EP1368124A1 - Dispositif de reception pour porte-objets - Google Patents

Dispositif de reception pour porte-objets

Info

Publication number
EP1368124A1
EP1368124A1 EP02759772A EP02759772A EP1368124A1 EP 1368124 A1 EP1368124 A1 EP 1368124A1 EP 02759772 A EP02759772 A EP 02759772A EP 02759772 A EP02759772 A EP 02759772A EP 1368124 A1 EP1368124 A1 EP 1368124A1
Authority
EP
European Patent Office
Prior art keywords
receiving
receiving device
slides
slide
elements
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP02759772A
Other languages
German (de)
English (en)
Inventor
Andreas Bosio
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Miltenyi Biotec GmbH
Original Assignee
Memorec Biotec GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Memorec Biotec GmbH filed Critical Memorec Biotec GmbH
Publication of EP1368124A1 publication Critical patent/EP1368124A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides

Definitions

  • the invention relates to a receiving device for slides.
  • Slides are, for example, thin glass plates on which samples to be examined are arranged. Frequently, two of these slides are placed on top of one another, so that the sample to be examined, which is in particular a sample liquid, is arranged between two slides.
  • slides can also be titre plates or similar plates with depressions that serve to hold individual samples.
  • slides are also used to examine tissue samples.
  • pieces of DNA i.e. Gene fragments
  • the DNA pieces are applied by dripping on with the aid of a robot.
  • the position of the individual pieces of DNA on the slide is thus known.
  • the pieces of DNA bind to the surface of the slide and adhere to it so that its position does not change in the subsequent examination process.
  • RNA is taken, for example, from a tumor to be examined.
  • the RNA is converted into DNA and then labeled with suitable markers, in particular fluorescent color markers.
  • suitable markers in particular fluorescent color markers.
  • a comparison sample with healthy tissue is generated.
  • the healthy DNA is also marked with a suitable marker.
  • the marker is preferably a fluorescent marker of a different color, so that the healthy tissue is marked with a green fluorescent marker and the tissue to be examined, for example removed from the tumor, is marked with a red color marker.
  • both samples are applied to the entire slide.
  • the DNA strands contained in the two samples bind firmly to their counterparts, i.e. the pieces of DNA on the surface of the slide.
  • the DNA contained in the samples is connected to the DNA pieces adhering to the slide in a hybridization process.
  • the slide is then washed so that only firmly adhering pieces of DNA and chained DNA from the two samples are left on the slide.
  • the slide After the slide has dried, it is sent to a detection process.
  • the individual positions of the slide, to which DNA fragments adhere, are examined with a suitable microscope.
  • the individual pieces of DNA are excited with laser light, for example, so that the fluorescent markers fluoresce in the corresponding color. If a specific position appears, to which a piece of DNA sticks, for example as a red spot, it can be concluded from this that this gene was active in the tumor tissue, but not in the healthy tissue. If a spot fluoresces green, it can be concluded that this gene was only active in the healthy tissue. When yellow fluorescence appeared, the corresponding gene was active in both tissues.
  • the above method can be used to diagnose which genes are active in a tumor, for example. From this, conclusions can be drawn about the type of tissue change and the like. pull.
  • the object of the invention is to improve the handling of slides.
  • a receiving device for specimen slides is provided according to the invention.
  • This has a base part, on which several slides can preferably be placed next to one another.
  • centering elements are connected to the bottom part.
  • the centering elements are arranged in such a way that a plurality of receiving areas are formed for receiving a slide in each case.
  • a slide, on which, for example, DNA pieces already adhere at predetermined positions, can thus be inserted into the receiving areas of the receiving device according to the invention.
  • the receiving device carrying a number of specimen slides can then be handled in a simple manner, for example with the aid of robot gripping arms or the like. become. Since the receiving device can be easily gripped, for example, on the centering elements or on the base part, the slide can be handled in a simple manner. In particular, the device according to the invention ensures that slides made of thin glass are not damaged during handling. Touching the samples already on slides is also avoided.
  • the base part is a base part, which is preferably continuous. The centering elements are then connected to the base part and form a plurality of receiving areas.
  • the centering elements are preferably designed like one-piece frame parts. Each slide is therefore surrounded by a frame. This has the advantage that the slide is arranged in a protected manner in the receiving area.
  • the frame part preferably has a height that is greater than the thickness of the specimen slide, so that the specimen slide is completely accommodated within the frame part. This prevents damage to the slide during handling of the receiving device and touches the surface of the slide.
  • a plurality of receiving areas are preferably formed by a single frame part. Each recording area is completely surrounded by a frame. A wall of the frame part provided between two adjacent specimen slides thus serves as part of the frame of two receiving areas.
  • the frame parts described above can each surround a receiving area, preferably completely as a circumferential centering frame. However, it can also be a matter of individual frame parts which do not surround the receiving areas, but rather, for example, only serve to receive corners of the specimen slide in order to center it in the receiving device. In this case, the insertion of the specimen slides into the receiving device may be facilitated.
  • the centering elements can also be chamfered to make it easier to insert the specimen slides into the receiving device. The chamfer is designed in such a way that the receiving area, in which a slide is held in each case, is extended upwards in order to facilitate the insertion of a slide.
  • the base part has flexible material at least in the receiving areas.
  • the entire bottom part consists of a continuous flexible material.
  • the slides rest on this flexible material.
  • the use of flexible material has the advantage that, for example when pipettes are touched by pipettes or the like. a certain suspension of the slides is guaranteed. This in turn reduces the risk of damage to the slides during the examination process.
  • the flexible material is a heat-permeable membrane.
  • the membrane is preferably designed such that temperatures from 4 ° C to 100 ° C can be transferred to the slide.
  • the base part is frame-shaped.
  • the base part in turn has a plurality of slide areas for receiving.
  • the base part is, for example, a rectangular frame which is divided into receiving areas by several intermediate webs is divided.
  • the receiving areas are essentially rectangular and have dimensions that are slightly smaller than the dimensions of standard slides, so that the slides can be inserted into the receiving areas, whereby they rest on the frame-shaped base part.
  • Centering elements are preferably designed as spring elements for holding the specimen slides on the frame-shaped base part.
  • the spring elements serve to clamp the slides and to fix them in their position. It is possible to design all centering elements as spring elements, so that the specimen slides are held in one plane. The slides are clamped between the spring elements.
  • the centering elements preferably only a part of the centering elements is designed as spring elements.
  • the remaining centering elements are then designed as stop elements in this embodiment.
  • the spring elements press the slide against appropriately arranged stop elements.
  • spring elements are provided which, in a horizontal plane, press the specimen slides in the x and y directions against stop elements oriented essentially perpendicular to the x and y directions.
  • a spring element for each direction and a stop element for each direction are preferably provided.
  • a more precise and defined position fixation can be achieved if, in particular, two spring elements are provided on the longer side of rectangular specimen slides, which preferably press the specimen slide against two stop elements oriented perpendicular to this direction.
  • the spring elements are preferably approaches which are connected resiliently to the base part.
  • the spring elements and that Base part in one piece, which is preferably an injection molded part.
  • This injection molded part preferably also includes the stop elements.
  • the outer dimensions of the receiving device correspond to standard dimensions of microtiter plates.
  • the receiving device has a width of 96 + 4 mm and a length of 148 + 4 mm.
  • FIG. 1 is a schematic plan view of a preferred embodiment of the receiving device according to the invention.
  • FIG. 2 shows a schematic sectional view along the line II-II in FIG. 1,
  • FIG. 3 shows a schematic top view of a further preferred embodiment of the receiving device according to the invention
  • FIG. 5 shows a single slide and insertable into the holding device shown in FIG. 1
  • Fig. 6 is a schematic perspective view of a
  • a receiving device 10 for a number of specimen slides 12 has a base part 14 (FIG. 2).
  • the base part 14 is connected to centering elements 16, 18, 20.
  • the centering elements 16, 18, 20 are arranged in such a way that four rectangular receiving areas 22 are formed, in each of which a specimen slide 12 can be arranged.
  • the centering elements 16, 18, 20 have two mutually opposite shorter side walls 16 and two longer side walls 18 which are arranged between the side walls 16 and are likewise opposite one another.
  • three intermediate walls 20 are provided between the side walls 18 and are arranged parallel to the shorter side walls 18.
  • the bottom part 14 which is designed as a membrane, is fastened.
  • the receiving areas 22 thus have a flexible membrane as the bottom, on each of which a slide 12 rests. It is thus possible to press against the membrane from below in FIG. 2, for example to align the slide horizontally.
  • a heating element can be applied to the slide 12 from below. Due to the flexibility of the membrane, the slide 12 lies flat against the surface of a flat heating element. This ensures a uniform and good heat transfer between the heating element and the slide.
  • the centering elements 16, 18, 20 are on their inner sides 23, i.e. on the sides pointing in the direction of the receiving areas 22, beveled. As a result, the opening of the individual receiving areas pointing upward in FIG. 2 is expanded upward. This facilitates the insertion of the specimen slides 12 into the receiving areas 22.
  • a frame-shaped base part 30 has a flat upper side 32 on which the specimen slides 12 are placed.
  • support lugs 34 are provided which protrude into the receiving areas 22, which are through openings.
  • further support lugs 36 are provided, which span a corner area of the receiving area 22 for manufacturing reasons.
  • the lugs 36 can also be designed in accordance with the support lugs 34.
  • the frame-shaped base part 30 has spring elements 38 as centering elements.
  • the spring elements 38 are integrally connected to the base part 30. Since the base part 30 and the spring elements 38 are preferably made of a plastic, in particular by injection molding, the spring elements 38 have a certain elasticity. Furthermore, the spring elements 38 have an obliquely running front edge 40, by means of which object slides resting on the upper side 32 are pressed downward in FIG. 4. The position of the specimen slides in FIG. 4 is thus also fixed in the z direction.
  • centering elements designed as stop elements 42, 44 are provided on the upper side 32 of the base part 30 for fixing the position of the specimen slides.
  • the spring elements 38 press a specimen slide in FIG. 2 in the x and y directions, two spring elements 38 being provided on the long side of a specimen slide 12. Opposite these two stop elements 44 are provided, against which the slides are pressed in the x direction. In the y-direction, the lower spring element 38 in FIG. 3 presses the specimen slide in the y-direction against the two stop elements 42.
  • the other stop elements 42 and 44 are safety stop elements which, inter alia, define the area to which the specimen slide is placed 12 can be placed.
  • the individual specimen slides 12 are used, for example, to examine DNA samples. For this purpose, each slide, as shown in FIG.
  • an inner area 46 for example, sample droplets are applied, which then firmly connect to the sample carrier 12.
  • the area 46 thus serves as a surface for arranging an array.
  • the area 46 is surrounded by a preferably 48 mm wide edge 48.
  • the edge 48 serves, for example, to ensure that all samples provided in the area 46 are at a sufficient distance from the outer edges 50 of the specimen slide 12.
  • seals of a washing head lowered onto the slide 12 or the like. be arranged in the region of the edge 48.
  • a region 52 which is separated from the edge 48 by the line 54 shown in FIG. 3, serves to grip the sample carrier. No samples are arranged in this area so that the slide can be traded in this area.
  • the grip area is required in order to be able to insert the slide into the receiving device according to the invention.
  • the recording device 10 according to the invention can be inserted into an examination device 56 (FIG. 4) which has, for example, a hybridization head.
  • the examination device 56 has a drawer-like receiving device 58 for receiving the receiving device 10.
  • the drawer can or the like according to a drawer of a CD player. be trained. When the drawer is open, the receiving device 10 carrying the specimen slides 12 is inserted into the drawer from above and then moved into the examination device 56.

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne un dispositif de réception pour porte-objets (12), qui présente une partie de base (14). Plusieurs éléments de centrage (16, 18, 20) sont reliés à la partie de base (14). Les éléments de centrage (16, 18, 20) sont disposés de manière que plusieurs zones de réception (22) soient conçues pour recevoir dans chaque cas un support d'objets (12). Les éléments de centrage (16, 18, 20) forment autour de chaque zone de réception (22) de préférence un cadre de centrage périphérique.
EP02759772A 2001-03-16 2002-03-15 Dispositif de reception pour porte-objets Withdrawn EP1368124A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10112682 2001-03-16
DE10112682 2001-03-16
PCT/EP2002/002899 WO2002081089A1 (fr) 2001-03-16 2002-03-15 Dispositif de reception pour porte-objets

Publications (1)

Publication Number Publication Date
EP1368124A1 true EP1368124A1 (fr) 2003-12-10

Family

ID=7677710

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02759772A Withdrawn EP1368124A1 (fr) 2001-03-16 2002-03-15 Dispositif de reception pour porte-objets

Country Status (5)

Country Link
US (2) US20040130712A1 (fr)
EP (1) EP1368124A1 (fr)
JP (2) JP4071635B2 (fr)
CA (1) CA2441439A1 (fr)
WO (1) WO2002081089A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10261533A1 (de) * 2002-12-23 2004-07-15 BioChip Technologies GmbH Gesellschaft für Entwicklung und Produktion von Detektionssystemen Vorrichtung und Verfahren zum Halten von Objektträgern
US7753457B2 (en) * 2003-06-12 2010-07-13 Cytyc Corporation Apparatus for removably retaining a slide within a cassette
GB0812541D0 (en) * 2008-07-09 2008-08-13 Hughes Thomas F Laboratory sample carrier try and tracking method
US8696991B1 (en) 2010-01-04 2014-04-15 Harold W. Howe Field deployable surface plasmon resonance based biosensor
US8695618B2 (en) 2010-12-22 2014-04-15 Carnegie Mellon University 3D chemical pattern control in 2D fluidics devices
US20130294826A1 (en) * 2012-05-04 2013-11-07 Advanced Cell Diagnostics, Inc. Lock-in slide rack
DE102012013678A1 (de) * 2012-07-11 2014-01-16 Euroimmun Medizinische Labordiagnostika Ag Verfahren und Analysevorrichtung zur mikroskopischen Untersuchung eines Gewebeschnittes oder eines Zellausstrichs
US9581800B2 (en) * 2014-11-21 2017-02-28 General Electric Company Slide holder for detection of slide placement on microscope
CA3077811C (fr) 2015-03-24 2024-02-27 Illumina, Inc. Procedes, ensembles de support, et systemes pour l'imagerie d'echantillons pour une analyse biologique ou chimique

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2761558A (en) * 1954-08-23 1956-09-04 Jr James Douglas Mclean Holder for microscope slides
US3746161A (en) * 1971-06-04 1973-07-17 Miles Lab Holder for flat rectangular objects
US3951512A (en) * 1974-11-04 1976-04-20 Tolles Walter E Microscope slide reference apparatus
US4159875A (en) * 1976-10-21 1979-07-03 Abbott Laboratories Specimen holder
JPS5650489Y2 (fr) * 1977-12-05 1981-11-26
US4209923A (en) * 1978-07-31 1980-07-01 Wendt David W Slide holder tray
US4589551A (en) * 1984-07-31 1986-05-20 Maclean-Fogg Company Container for handling, transportation and storage of microscope slides
US4836667A (en) * 1986-05-06 1989-06-06 Slidex Corporation Microscope
US5428690A (en) * 1991-09-23 1995-06-27 Becton Dickinson And Company Method and apparatus for automated assay of biological specimens
US5439649A (en) * 1993-09-29 1995-08-08 Biogenex Laboratories Automated staining apparatus
US5595710A (en) * 1995-05-25 1997-01-21 Intelligent Medical Imaging, Inc. Medical slide holder
US6118582A (en) * 1999-07-12 2000-09-12 Immuno Concepts, Inc. Slide holder
EP1798550B1 (fr) 2005-12-13 2018-07-25 Lothgenoten B.V. Dispositif pour inspecter l'intérieur d'un matériau

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
None *
See also references of WO02081089A1 *

Also Published As

Publication number Publication date
CA2441439A1 (fr) 2002-10-17
US20040130712A1 (en) 2004-07-08
JP2004528555A (ja) 2004-09-16
US7554657B2 (en) 2009-06-30
JP4071635B2 (ja) 2008-04-02
WO2002081089A1 (fr) 2002-10-17
JP2008070386A (ja) 2008-03-27
US20070030480A1 (en) 2007-02-08

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