EP1313562B1 - Multiwell filtrationsplatte und verfahren zu ihrer herstellung - Google Patents

Multiwell filtrationsplatte und verfahren zu ihrer herstellung Download PDF

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Publication number
EP1313562B1
EP1313562B1 EP01964906A EP01964906A EP1313562B1 EP 1313562 B1 EP1313562 B1 EP 1313562B1 EP 01964906 A EP01964906 A EP 01964906A EP 01964906 A EP01964906 A EP 01964906A EP 1313562 B1 EP1313562 B1 EP 1313562B1
Authority
EP
European Patent Office
Prior art keywords
outlet
filtration plate
well
multiwell filtration
connecting piece
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP01964906A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP1313562A1 (de
Inventor
Hans-Jürgen Hoffmann
Timo Hillebrand
Peter Bendzko
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AHN Biotechnology GmbH
Invitek Gesellschaft fur Biotechnik & Biodesig
Original Assignee
AHN Biotechnology GmbH
Invitek Gesellschaft fuer Biotechnik und Biodesign mbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AHN Biotechnology GmbH, Invitek Gesellschaft fuer Biotechnik und Biodesign mbH filed Critical AHN Biotechnology GmbH
Publication of EP1313562A1 publication Critical patent/EP1313562A1/de
Application granted granted Critical
Publication of EP1313562B1 publication Critical patent/EP1313562B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration

Definitions

  • the invention relates to a new multi-well filtration plate, preferably in 96-well or in 384-well format, consisting of two separate parts, which are firmly and tightly interconnected.
  • the first part represents a sample receiving part with filter plates, and the second part acts as a discharge part after filtration.
  • This plate according to the invention is particularly suitable for high-throughput applications in nucleic acid technology and for protein purification and analysis.
  • the new multi-well filtration plate consists of two tightly connected individual parts.
  • the upper part of the plate is defined as a sample receiving part 1, the lower part of the plate as a discharge part 2 with filter 3.
  • This outlet part 2 comprises for each well according to the invention shaped outlets 4, via which the filtrate in a collecting vessel, such as. a deep-well plate, for further determination runs.
  • the outlet part is thus vzw by the correspondingly shaped outlet spout 4, depending on the plate. 96 or 384.
  • outlet nozzles have an inner angle of inclination of 15-25 °, preferably 20 ° and have at the junction with the upper part each about 1.0-1.5mm, preferably 1.0mm thick support paragraph (seal paragraph) 5 for stabilizing the filter cartridge 3 , Their length is about 5-15mm.
  • 96-well plates have spouts with a length of 10-15 mm, vzw. they have a length of 12 mm.
  • 384-well plates have spout with a length of about 5-8 mm, vzw. they have a length of 5 to 6 mm. Possibly. the filtration plate is coupled to the outlet vessel.
  • outlet nozzles are usually round, in a further embodiment, they may be formed at the outlet end or starry with at least 8 openings.
  • the internal tilt angle of 15-25 °, preferably 20 °, and the special outlet design allow easy and complete leakage of the filtered sample into the collecting vessel, eg the deep-well plate (minimizing the dead volume, almost 0).
  • the length of the outlet with vzw. about 12 mm for 96-well plates and with vzw. 5 to 6 mm for 384 well plates can avoid the highly problematic cross-contamination
  • Multi-well filtration plate according to the invention can also be used for applications in PCR-based infection diagnostics.
  • the extension of the outlet connection furthermore has the consequence that, in contrast to previously known filtration plates, the filtration plate according to the invention has an increased capacity of uptake volume with a chamber volume of more than 1 ml for 96 well plates and approx. 300 ⁇ l for 384 well plates, ie Overall, larger sample volumes can be processed than previously customary. This also leads to more reproducible results. This applies in particular to the application of the 384-well plates, which is very much desired by the person skilled in the art, and whose throughput could thus be increased by 4-6 times.
  • the invention also provides the process for producing the new multi-well filtration plate.
  • Both parts of the multi-well filtration plate are firmly connected to each other in a special production process via a hydraulic compression.
  • the compression takes place in each case at the recesses with the outlet connection (96 or 384).
  • Toollike, the inner Verpressungskanten are sharpened so that during the compression, in which the filter element 3, such. B. a filter mat is placed between upper 1 and lower part 2, the wells corresponding filters are punched clean.
  • These punched filter cartridges are pressed with the upper part in the lower part and lie down on the support shoulder 5 in the lower part.
  • By hydraulic pressure plate upper part, filter plate and plate bottom are firmly and tightly pressed together.
  • the production of the multi-well filtration plate is preferably carried out by injection molding with carefully adjusted injection molding parameters.
  • the filtration panels of the invention are made of known materials, e.g. Polystyrene or polypropylene.
  • the injection molding material used is preferably a high-quality polystyrene. Since only precisely fitting injection-molded parts are used, the compression takes place without sealing lips, etc.
  • the invention ideally solves all existing problems.
  • the plate is produced by means of a new technological process, which makes it possible to transport a filter material into the multi-well filtration plate in a highly efficient and cost-effective manner.
  • the novel multi-well filtration plate is constructed in its constructive dimensioning that can be eliminated on the shape of the spout previously known cross contamination.
  • External dimensions (width and length) of the plate and the arrangement of the wells correspond to a normal microtitration plate, with a further advantage in increasing the volume of the reaction cavities to about 1 ml (96 well) or to about 300 ⁇ l (384 well).
  • the plate is also easy to use in protein purification and analysis.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Filtering Materials (AREA)
EP01964906A 2000-08-25 2001-08-22 Multiwell filtrationsplatte und verfahren zu ihrer herstellung Expired - Lifetime EP1313562B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10041825A DE10041825A1 (de) 2000-08-25 2000-08-25 Multiwell Filtrationsplatte und Verfahren zu ihrer Herstellung
DE10041825 2000-08-25
PCT/DE2001/003137 WO2002016035A1 (de) 2000-08-25 2001-08-22 Multiwell filtrationsplatte und verfahren zu ihrer herstellung

Publications (2)

Publication Number Publication Date
EP1313562A1 EP1313562A1 (de) 2003-05-28
EP1313562B1 true EP1313562B1 (de) 2006-10-25

Family

ID=7653785

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01964906A Expired - Lifetime EP1313562B1 (de) 2000-08-25 2001-08-22 Multiwell filtrationsplatte und verfahren zu ihrer herstellung

Country Status (10)

Country Link
US (1) US6830732B1 (es)
EP (1) EP1313562B1 (es)
JP (1) JP2004506918A (es)
CN (1) CN1471432A (es)
AT (1) ATE343426T1 (es)
AU (1) AU2001285706A1 (es)
DE (2) DE10041825A1 (es)
DK (1) DK1313562T3 (es)
ES (1) ES2275719T3 (es)
WO (1) WO2002016035A1 (es)

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* Cited by examiner, † Cited by third party
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GB2372464B (en) * 2001-02-22 2003-05-14 Vivascience Ltd Method of isolating a charged compound
KR20090045426A (ko) * 2001-05-31 2009-05-07 폴 코포레이션 유체처리용 웰
WO2004008108A1 (en) * 2002-07-15 2004-01-22 Avantium International B.V. A system for the preparation of multiple solid state samples, in particular for spectroscopic and microscopic analysis
CA2499913A1 (en) * 2002-10-10 2004-04-22 Irm, Llc Capacity altering device, holder and methods of sample processing
DE10310025A1 (de) * 2003-03-06 2004-09-16 Rheinisch-Westfälisch- Technische Hochschule Aachen Vorrichtung zur simultanen Aufnahme von mehreren Gegenständen
US7063216B2 (en) 2003-09-04 2006-06-20 Millipore Corporation Underdrain useful in the construction of a filtration device
US8968679B2 (en) * 2005-05-19 2015-03-03 Emd Millipore Corporation Receiver plate with multiple cross-sections
WO2008157278A1 (en) * 2007-06-15 2008-12-24 Smithkline Beecham Corporation Antibody formulations
US20100089938A1 (en) * 2008-04-11 2010-04-15 Arta Motadel Pipette tip handling devices and methods
WO2009126945A2 (en) 2008-04-11 2009-10-15 Pelican Group Holdings, Inc. Pipette tip handling devices and methods
USD697227S1 (en) 2009-04-11 2014-01-07 Biotix, Inc. Pipette tip handling device set
US8590736B2 (en) 2009-04-11 2013-11-26 Biotix, Inc. Automated pipette tip loading devices and methods
DE102009057223B4 (de) * 2009-12-05 2016-03-24 Chemagen Biopolymer-Technologie Aktiengesellschaft Probengefäßmatrix und deren Herstellungsverfahren
WO2014018751A1 (en) * 2012-07-27 2014-01-30 Emory University Cell filtration device

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US3295686A (en) * 1965-05-20 1967-01-03 Rockridge Lab Filter unit
DE3214287A1 (de) * 1981-04-23 1982-12-02 Günther Prof. Dipl.-Ing. Dr. 8047 Graz Knapp Roehrchen zur durchfuehrung eines radioimmunoassays
US4948442A (en) 1985-06-18 1990-08-14 Polyfiltronics, Inc. Method of making a multiwell test plate
US4948564A (en) 1986-10-28 1990-08-14 Costar Corporation Multi-well filter strip and composite assemblies
US4902481A (en) 1987-12-11 1990-02-20 Millipore Corporation Multi-well filtration test apparatus
DE3843610A1 (de) * 1988-01-13 1989-07-27 Stephan Dr Diekmann Trenn- oder reaktionssaeuleneinheit
DE3804429A1 (de) * 1988-02-12 1989-08-24 Schleicher & Schuell Gmbh Einmalfilter
US5108704A (en) 1988-09-16 1992-04-28 W. R. Grace & Co.-Conn. Microfiltration apparatus with radially spaced nozzles
JPH02151769A (ja) * 1988-12-02 1990-06-11 Jeol Ltd 反応容器
SE9000650L (sv) * 1989-02-28 1990-08-29 Asahi Optical Co Ltd Separation av celler eller virus
US5141719A (en) 1990-07-18 1992-08-25 Bio-Rad Laboratories, Inc. Multi-sample filtration plate assembly
US5264184A (en) * 1991-03-19 1993-11-23 Minnesota Mining And Manufacturing Company Device and a method for separating liquid samples
US5205989A (en) * 1991-09-18 1993-04-27 Minnesota Mining And Manufacturing Company Multi-well filtration apparatus
US5368729A (en) * 1993-07-23 1994-11-29 Whatman, Inc. Solid phase extraction device
US5620662A (en) 1993-08-23 1997-04-15 Brandeis University Temporary liquid storage cavities in a centrifuge tube lid
US5595653A (en) * 1994-07-15 1997-01-21 Cera, Inc. Microcolumn for extraction of analytes from liquids
US5846493A (en) * 1995-02-14 1998-12-08 Promega Corporation System for analyzing a substance from a solution following filtering of the substance from the solution
DE29505652U1 (de) * 1995-04-01 1996-04-25 Boehringer Mannheim Gmbh, 68305 Mannheim Gefäß zur kontaminationsreduzierten Behandlung von Flüssigkeiten
US5874004A (en) * 1996-06-19 1999-02-23 Sheila H. Dewitt Phase separation filter device
US5792430A (en) * 1996-08-12 1998-08-11 Monsanto Company Solid phase organic synthesis device with pressure-regulated manifold
US6027694A (en) 1996-10-17 2000-02-22 Texperts, Inc. Spillproof microplate assembly
US6054100A (en) * 1996-11-18 2000-04-25 Robbins Scientific Corporation Apparatus for multi-well microscale synthesis
US5888831A (en) * 1997-03-05 1999-03-30 Gautsch; James W. Liquid-sample-separation laboratory device and method particularly permitting ready extraction by syringe of the separated liquid sample
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Also Published As

Publication number Publication date
US6830732B1 (en) 2004-12-14
DE10041825A1 (de) 2002-03-07
ES2275719T3 (es) 2007-06-16
CN1471432A (zh) 2004-01-28
WO2002016035A1 (de) 2002-02-28
EP1313562A1 (de) 2003-05-28
JP2004506918A (ja) 2004-03-04
DK1313562T3 (da) 2007-02-26
DE50111329D1 (de) 2006-12-07
AU2001285706A1 (en) 2002-03-04
ATE343426T1 (de) 2006-11-15

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