EP1313562B1 - Multiwell filtration plate and method for producing the same - Google Patents

Multiwell filtration plate and method for producing the same Download PDF

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Publication number
EP1313562B1
EP1313562B1 EP01964906A EP01964906A EP1313562B1 EP 1313562 B1 EP1313562 B1 EP 1313562B1 EP 01964906 A EP01964906 A EP 01964906A EP 01964906 A EP01964906 A EP 01964906A EP 1313562 B1 EP1313562 B1 EP 1313562B1
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EP
European Patent Office
Prior art keywords
outlet
filtration plate
well
multiwell filtration
connecting piece
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EP01964906A
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German (de)
French (fr)
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EP1313562A1 (en
Inventor
Hans-Jürgen Hoffmann
Timo Hillebrand
Peter Bendzko
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AHN Biotechnology GmbH
Invitek Gesellschaft fur Biotechnik & Biodesig
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AHN Biotechnology GmbH
Invitek Gesellschaft fuer Biotechnik und Biodesign mbH
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration

Definitions

  • the invention relates to a new multi-well filtration plate, preferably in 96-well or in 384-well format, consisting of two separate parts, which are firmly and tightly interconnected.
  • the first part represents a sample receiving part with filter plates, and the second part acts as a discharge part after filtration.
  • This plate according to the invention is particularly suitable for high-throughput applications in nucleic acid technology and for protein purification and analysis.
  • the new multi-well filtration plate consists of two tightly connected individual parts.
  • the upper part of the plate is defined as a sample receiving part 1, the lower part of the plate as a discharge part 2 with filter 3.
  • This outlet part 2 comprises for each well according to the invention shaped outlets 4, via which the filtrate in a collecting vessel, such as. a deep-well plate, for further determination runs.
  • the outlet part is thus vzw by the correspondingly shaped outlet spout 4, depending on the plate. 96 or 384.
  • outlet nozzles have an inner angle of inclination of 15-25 °, preferably 20 ° and have at the junction with the upper part each about 1.0-1.5mm, preferably 1.0mm thick support paragraph (seal paragraph) 5 for stabilizing the filter cartridge 3 , Their length is about 5-15mm.
  • 96-well plates have spouts with a length of 10-15 mm, vzw. they have a length of 12 mm.
  • 384-well plates have spout with a length of about 5-8 mm, vzw. they have a length of 5 to 6 mm. Possibly. the filtration plate is coupled to the outlet vessel.
  • outlet nozzles are usually round, in a further embodiment, they may be formed at the outlet end or starry with at least 8 openings.
  • the internal tilt angle of 15-25 °, preferably 20 °, and the special outlet design allow easy and complete leakage of the filtered sample into the collecting vessel, eg the deep-well plate (minimizing the dead volume, almost 0).
  • the length of the outlet with vzw. about 12 mm for 96-well plates and with vzw. 5 to 6 mm for 384 well plates can avoid the highly problematic cross-contamination
  • Multi-well filtration plate according to the invention can also be used for applications in PCR-based infection diagnostics.
  • the extension of the outlet connection furthermore has the consequence that, in contrast to previously known filtration plates, the filtration plate according to the invention has an increased capacity of uptake volume with a chamber volume of more than 1 ml for 96 well plates and approx. 300 ⁇ l for 384 well plates, ie Overall, larger sample volumes can be processed than previously customary. This also leads to more reproducible results. This applies in particular to the application of the 384-well plates, which is very much desired by the person skilled in the art, and whose throughput could thus be increased by 4-6 times.
  • the invention also provides the process for producing the new multi-well filtration plate.
  • Both parts of the multi-well filtration plate are firmly connected to each other in a special production process via a hydraulic compression.
  • the compression takes place in each case at the recesses with the outlet connection (96 or 384).
  • Toollike, the inner Verpressungskanten are sharpened so that during the compression, in which the filter element 3, such. B. a filter mat is placed between upper 1 and lower part 2, the wells corresponding filters are punched clean.
  • These punched filter cartridges are pressed with the upper part in the lower part and lie down on the support shoulder 5 in the lower part.
  • By hydraulic pressure plate upper part, filter plate and plate bottom are firmly and tightly pressed together.
  • the production of the multi-well filtration plate is preferably carried out by injection molding with carefully adjusted injection molding parameters.
  • the filtration panels of the invention are made of known materials, e.g. Polystyrene or polypropylene.
  • the injection molding material used is preferably a high-quality polystyrene. Since only precisely fitting injection-molded parts are used, the compression takes place without sealing lips, etc.
  • the invention ideally solves all existing problems.
  • the plate is produced by means of a new technological process, which makes it possible to transport a filter material into the multi-well filtration plate in a highly efficient and cost-effective manner.
  • the novel multi-well filtration plate is constructed in its constructive dimensioning that can be eliminated on the shape of the spout previously known cross contamination.
  • External dimensions (width and length) of the plate and the arrangement of the wells correspond to a normal microtitration plate, with a further advantage in increasing the volume of the reaction cavities to about 1 ml (96 well) or to about 300 ⁇ l (384 well).
  • the plate is also easy to use in protein purification and analysis.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Filtering Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

The invention relates to a new multiwell filtration plate for high throughput applications in nucleic acid technology, preferably in a 96-well or 384-well format, consisting of two individual parts, which are firmly and tightly connected together. The upper part of the plate is a sample holder 1 for holding a sample to be filtered. The lower part of the plate is an outlet part with a filter insert for receiving a sample from the sample holder part and filtering the, sample through the filter insert.

Description

Die Erfindung betrifft eine neue Multiwell-Filtrationsplatte, vorzugsweise im 96-Well-oder im 384-Well-Format, bestehend aus zwei Einzelteilen, die fest und dicht miteinander verbunden sind. Der erste Teil stellt einen Probeaufnahmeteil mit Filterplättchen dar, und der zweite Teil fungiert als Auslaufteil nach erfolgter Filtration. Diese erfindungsgemäße Platte ist insbesondere für Hochdurchsatzapplikationen in der Nukleinsäuretechnik sowie für die Proteinaufreinigung und -analytik geeignet.The invention relates to a new multi-well filtration plate, preferably in 96-well or in 384-well format, consisting of two separate parts, which are firmly and tightly interconnected. The first part represents a sample receiving part with filter plates, and the second part acts as a discharge part after filtration. This plate according to the invention is particularly suitable for high-throughput applications in nucleic acid technology and for protein purification and analysis.

In den letzten Jahren zeichnete sich ein zunehmender Trend zur Automatisierung bei der Isolierung und Aufreinigung von Nukleinsäuren ab. Die Ursachen liegen darin begründet, dass sich in allen Forschungsfeldern der modernen Biotechnologie molekularbiologische Verfahren immer stärker etablieren. Nicht zuletzt durch die DNA-Sequenzierung innerhalb weltweiter Genomprojekte (z.B. Humanes Genomprojekt) ist eine Automatisierung der Isolierung und Aufreinigung von Plasmid-DNA eine zwingende Voraussetzung geworden. Die Notwendigkeit der Entwicklung von Automatisierungsvarianten beschränkt sich dabei nicht nur auf den Bereich der Plasmid-DNA-Isolierung. Mehr und mehr gewinnt auch die automatisierte Isolierung genomischer DNA aus unterschiedlichsten Ausgangsmaterialien und Mengen sowie die Isolierung von RNA an Bedeutung. Dies umfasst alle Bereiche der molekularen Basisforschung und zunehmend auch den Diagnostikbereich. Verfahren der automatisierten Isolierung von Nukleinsäuren werden z.Z. über Mikrotestplatten mit eingebauten Filtermaterialien realisiert. Dabei sind z.Z. Verwendung findende Formate sog. 96-Well bzw. 384-Well-Mikrotestplatten.In recent years, there has been an increasing trend toward automation in the isolation and purification of nucleic acids. The reasons lie in the fact that in all research fields of modern biotechnology molecular biological procedures are becoming increasingly established. Not least through DNA sequencing within global genome projects (e.g., human genome project), automation of isolation and purification of plasmid DNA has become a mandatory requirement. The need for the development of automation variants is not limited to the field of plasmid DNA isolation. Increasingly, the automated isolation of genomic DNA from a wide variety of starting materials and quantities as well as the isolation of RNA are gaining in importance. This encompasses all areas of basic molecular research and increasingly also the diagnostics sector. Methods of automated isolation of nucleic acids are currently being used. implemented using microplates with built-in filter materials. There are z.Z. Forms used are so-called 96-well or 384-well microplates.

Die z.Z. verfügbaren 96-Well-Mikrotestplatten mit Filtermaterial sind allerdings für Hochdurchsatzapplikationen extrem teuer. Dies resultiert aus den relativ komplizierten Herstellungsverfahren zur Einbringung des Filtermaterials in die Mikrotestplatte (US-A 4,948,442). Darüber hinaus realisiert bisher keine der bekannten Mikrotestplatten mit Filtereinsatz einen ausreichend hohen Schutz vor Kreuzkontaminationen von Proben bei Anwendung zur Isolierung und Aufreinigung von Nukleinsäuren. Die Ursache liegt in den viel zu kurzen Auslaufstutzen am Boden der Multiwell-Filtrationsplatten. Ein weiteres Problem sind die geringen Volumina der Reaktionskavitäten, z.B. beschrieben im EP-A1 0,098,534. Auch die bekannten 384-Well-Platten des Standes der Technik gestatten nur den Durchsatz geringer Volumina im jeweiligen Analyseverfahren, diese Volumina liegen bei ca. 50-80 µl. Für die Durchführung von Analysen, insbesondere zur automatischen Analyse, und für den Einsatz in Kombination mit Laborrobotem sind in den meisten Fällen jedoch Testplatten, die einen Durchsatz mit großen Volumina erlauben, erforderlich.However, the 96-well microtiter plates with filter material currently available are extremely expensive for high-throughput applications. This results from the relatively complicated manufacturing process for introducing the filter material into the microplate (US-A 4,948,442). Moreover, so far none of the known microtest plates with filter insert realized a sufficiently high protection against cross-contamination of samples when used for the isolation and purification of nucleic acids. The reason lies in the much too short discharge spigot at the bottom of the multiwell filtration plates. Another problem is the low volumes of the reaction cavities, for example described in EP-A1 0,098,534. Even the known 384-well plates of the prior art allow only the throughput of small volumes in the respective analysis method, these volumes are about 50-80 ul. For carrying out analyzes, in particular for automatic Analysis, and for use in combination with laboratory robots, however, test plates that allow high volume throughput are required in most cases.

Der Erfindung lag deshalb die Aufgabe zugrunde, eine Multiwell-Filtrationsplatte bereitzustellen, die Schutz vor Kreuzkontaminationen der Proben gewährt, die kostengünstig herstellbar und vielschichtig einsetzbar ist, wie z.B. für Hochdurchsatzapplikationen in der Nukleinsäuretechnik und in der Proteintechnik.It is an object of the present invention to provide a multi-well filtration plate which provides protection against cross-contamination of the samples, which is inexpensive to produce and versatile in use, e.g. for high-throughput applications in nucleic acid technology and in protein technology.

Die neue Multiwell-Filtrationsplatte besteht aus zwei dicht verbundenen Einzelteilen. Der obere Teil der Platte wird als Probenaufnahmeteil 1 definiert, der untere Teil der Platte als Auslaufteil 2 mit Filter 3. Dieser Auslaufteil 2 umfaßt für jedes Well erfindungsgemäß geformte Auslaufstutzen 4, über die das Filtrat in ein Auffanggefäß, wie z.B. eine Deep-Well-Platte, zur weiteren Bestimmung läuft. Im wesentlichen ist der Auslaufteil also durch die entsprechend besonders geformten Auslaufstutzen 4, je nach Platte vzw. 96 oder 384, gekennzeichnet.The new multi-well filtration plate consists of two tightly connected individual parts. The upper part of the plate is defined as a sample receiving part 1, the lower part of the plate as a discharge part 2 with filter 3. This outlet part 2 comprises for each well according to the invention shaped outlets 4, via which the filtrate in a collecting vessel, such as. a deep-well plate, for further determination runs. In essence, the outlet part is thus vzw by the correspondingly shaped outlet spout 4, depending on the plate. 96 or 384.

Diese Auslaufstutzen besitzen einen inneren Neigungswinkel von 15-25°, vorzugsweise 20° und weisen an der Anschlußstelle zum Oberteil jeweils einen ca. 1,0-1,5mm, vorzugsweise 1,0mm starken Auflageabsatz (Dichtungsabsatz) 5 zur Stabilisierung des Filtereinsatzes 3 auf. Ihre Länge beträgt ca. 5-15mm. 96-Well-Platten weisen Auslaufstutzen mit einer Länge von 10-15 mm auf, vzw. besitzen sie eine Länge von 12 mm. 384-Well-Platten weisen Auslaufstutzen mit einer Länge von ca. 5-8 mm auf, vzw. besitzen sie eine Länge von 5 bis 6 mm. Ggf. ist die Filtrationsplatte an das Auslaufgefäß gekoppelt.These outlet nozzles have an inner angle of inclination of 15-25 °, preferably 20 ° and have at the junction with the upper part each about 1.0-1.5mm, preferably 1.0mm thick support paragraph (seal paragraph) 5 for stabilizing the filter cartridge 3 , Their length is about 5-15mm. 96-well plates have spouts with a length of 10-15 mm, vzw. they have a length of 12 mm. 384-well plates have spout with a length of about 5-8 mm, vzw. they have a length of 5 to 6 mm. Possibly. the filtration plate is coupled to the outlet vessel.

Desweiteren sind die Auslaufstutzen in der Regel rund, in einer weiteren Ausführungsvariante können sie am Auslaufende oder ganz sternenförmig mit mindestens 8 Öffnungen ausgebildet sein.Furthermore, the outlet nozzles are usually round, in a further embodiment, they may be formed at the outlet end or starry with at least 8 openings.

Der innere Neigungswinkel von 15-25°, vorzugsweise 20°, und das besondere Auslaufdesign ermöglichen ein problemloses und vollständiges Auslaufen der gefilterten Probe in das Auffanggefäß, z.B. die Deep-Well Platte (Minimierung des Todvolumens, nahezu 0). Durch die Länge der Auslaufstutzen mit vzw. ca. 12 mm für 96-Well-Platten und mit vzw. 5 bis 6 mm für 384 Well-Platten (bekannte Mikrotiterplatten besitzen Ausläufe mit einer maximalen Länge von 9 mm (96 Well) und < 5 mm (384 Well) können die hochproblematischen Kreuzkontaminationen vermieden werden. Damit ist die erfindungsgemäße Multiwell-Filtrationsplatte auch für Anwendungen in der PCRbasierenden Infektionsdiagnostik nutzbar.The internal tilt angle of 15-25 °, preferably 20 °, and the special outlet design allow easy and complete leakage of the filtered sample into the collecting vessel, eg the deep-well plate (minimizing the dead volume, almost 0). By the length of the outlet with vzw. about 12 mm for 96-well plates and with vzw. 5 to 6 mm for 384 well plates (known microtiter plates have spouts with a maximum length of 9 mm (96 wells) and <5 mm (384 wells) can avoid the highly problematic cross-contamination Multi-well filtration plate according to the invention can also be used for applications in PCR-based infection diagnostics.

Die Verlängerung der Auslaufstutzen hat desweiteren zur Folge, dass im Gegensatz zu bislang bekannten Filtrationsplatten die erfindungsgemäße Filtrationsplatte eine vergrößerte Aufnahmevolumen-Kapazität mit einem Kammervolumen von über 1 ml für 96 Well Platten und ca. 300 µl für 384 Well-Platten besitzt, d.h., es können insgesamt größere Probenvolumina als bislang üblich verarbeitet werden. Das führt somit auch zu reproduzierbareren Ergebnissen. Besonders gilt dies für die vom Fachmann sehr gewünschte Anwendung der 384-Well-Platten, deren Durchsatz damit um das 4―6 fache gesteigert werden konnte.The extension of the outlet connection furthermore has the consequence that, in contrast to previously known filtration plates, the filtration plate according to the invention has an increased capacity of uptake volume with a chamber volume of more than 1 ml for 96 well plates and approx. 300 μl for 384 well plates, ie Overall, larger sample volumes can be processed than previously customary. This also leads to more reproducible results. This applies in particular to the application of the 384-well plates, which is very much desired by the person skilled in the art, and whose throughput could thus be increased by 4-6 times.

Gegenstand der Erfindung ist auch das Verfahren zur Herstellung der neuen Multiwell-Filtrationsplatte. Beide Teile der Multiwell-Filtrationsplatte werden in einem besonderen Produktionsablauf fest miteinander über eine hydraulische Verpressung verbunden. Die Verpressung erfolgt jeweils an den Vertiefungen mit den Auslaufstutzen (96 bzw. 384). Werkzeugmäßig sind die inneren Verpressungskanten so geschärft, dass bei der Verpressung, bei der der Filtereinsatz 3 , wie z. B. eine Filtermatte, zwischen Ober- 1 und Unterteil 2 gelegt wird, den Vertiefungen entsprechende Filter sauber gestanzt werden. Diese ausgestanzten Filtereinsätze werden mit dem Oberteil in das Unterteil gepresst und legen sich auf den Auflageabsatz 5 im Unterteil auf. Durch hydraulischen Druck werden Plattenoberteil, Filterplättchen und Plattenunterteil fest und dicht miteinander verpresst.The invention also provides the process for producing the new multi-well filtration plate. Both parts of the multi-well filtration plate are firmly connected to each other in a special production process via a hydraulic compression. The compression takes place in each case at the recesses with the outlet connection (96 or 384). Toollike, the inner Verpressungskanten are sharpened so that during the compression, in which the filter element 3, such. B. a filter mat is placed between upper 1 and lower part 2, the wells corresponding filters are punched clean. These punched filter cartridges are pressed with the upper part in the lower part and lie down on the support shoulder 5 in the lower part. By hydraulic pressure plate upper part, filter plate and plate bottom are firmly and tightly pressed together.

Eine solche Verpressung wird erfindungsgemäß möglich, da Unterteil und Oberteil der neuen Multiwell-Filtrationsplatten in zwei absoluten Präzisionswerkzeugen (Heißkanaltechnik), die exakt aufeinander abgestimmt sind, mit einer maximalen Toleranz von 1/1000 mm, gefertigt werden. Scharfe Schneidkanten an den Verpressungsteilen der beiden Plattenteile ermöglichen das Ausstanzen von Einzelfiltern aus einer Filterplatte in einem Arbeitsgang mit dem eigentlichen Verpressen. Das Verpressen erfolgt direkt, d.h. es sind gemäß der Erfindung weder Nut noch Feder (wie aus dem allgemeinen Stand der Technik bekannt) erforderlich, was einen erheblichen technologischen Vorteil bedeutet.Such compression is possible according to the invention, since lower part and upper part of the new multi-well filtration plates in two absolute precision tools (hot runner technology), which are precisely matched, with a maximum tolerance of 1/1000 mm, are made. Sharp cutting edges on the pressing parts of the two plate parts allow the punching of individual filters from a filter plate in one operation with the actual pressing. The pressing is done directly, ie there are according to the invention, neither groove nor spring (as known from the general state of the art) required, which means a significant technological advantage.

Die Herstellung der Multiwell-Filtrationsplatte erfolgt bevorzugt im Spritzgußverfahren mit sorgfältig eingestellten Spritzgußparametern. Die erfindungsgemäßen Filtrationsplatten bestehen aus an sich bekannten Materialien, wie z.B. Polystyrol oder Polypropylen. Das eingesetzte Spritzgußmaterial ist bevorzugt ein hochwertiges Polystyrol. Da nur passgenaue Spritzgussteile verwendet werden, erfolgt die Verpressung ohne Dichtungslippen u.ä..The production of the multi-well filtration plate is preferably carried out by injection molding with carefully adjusted injection molding parameters. The filtration panels of the invention are made of known materials, e.g. Polystyrene or polypropylene. The injection molding material used is preferably a high-quality polystyrene. Since only precisely fitting injection-molded parts are used, the compression takes place without sealing lips, etc.

Die Erfindung löst in idealster Weise alle bestehenden Probleme. Die Herstellung der Platte erfolgt mittels eines neuen technologischen Verfahrens, welches hocheffizient und preiswert die Verbringung eines Filtermaterials in die Multiwell-Filtrationsplatte ermöglicht. Weiterhin ist die neuartige Multiwell-Filtrationsplatte in ihrer konstruktiven Dimensionierung so aufgebaut, dass über die Form der Auslaufstutzen bisher bekannte Kreuzkontaminationen beseitigt werden können. Außenmaße (Breite und Länge) der Platte und die Anordnung der Vertiefungen (vorzugsweise 8 x 12 Matrix) entsprechen einer normalen Mikrotitrationsplatte, wobei ein weiterer Vorteil in der Erhöhung der Volumina der Reaktionskavitäten auf ca. 1 ml (96 Well) bzw. auf ca. 300 µl (384 Well) besteht. Damit können prinzipiell alle Standardapplikationen der automatisierten Isolierung und Aufreinigung von Nukleinsäuren problemlos mit einer Platte realisiert werden. Des weiteren ist die Platte ebenfalls in der Proteinaufreinigung und - analytik problemlos einsetzbar.The invention ideally solves all existing problems. The plate is produced by means of a new technological process, which makes it possible to transport a filter material into the multi-well filtration plate in a highly efficient and cost-effective manner. Furthermore, the novel multi-well filtration plate is constructed in its constructive dimensioning that can be eliminated on the shape of the spout previously known cross contamination. External dimensions (width and length) of the plate and the arrangement of the wells (preferably 8 x 12 matrix) correspond to a normal microtitration plate, with a further advantage in increasing the volume of the reaction cavities to about 1 ml (96 well) or to about 300 μl (384 well). Thus, in principle, all standard applications of automated isolation and purification of nucleic acids can be easily realized with a plate. Furthermore, the plate is also easy to use in protein purification and analysis.

Anschließend wird die Erfindung an den folgenden Beispielen näher erläutert..

Fig. 1
Gesamtansicht einer 96-Well-Vorrichtung
Fig. 2a
Längsschnitt der einzelnen Vorrichtungsteile
Fig. 2b
Längsschnitt mit Filtermembran
Fig. 3
Auslaufstutzen als Querschnitt
Subsequently, the invention will be explained in more detail by the following examples.
Fig. 1
Overall view of a 96-well device
Fig. 2a
Longitudinal section of the individual device parts
Fig. 2b
Longitudinal section with filter membrane
Fig. 3
Outlet as a cross section

BezugszeichenlisteLIST OF REFERENCE NUMBERS

11
Probeaufnahmeteil (Oberteil)Sample receiving part (upper part)
22
Auslaufteil (Unterteil)Outlet part (lower part)
33
Filtereinsatzfilter cartridge
44
Auslaufstutzenoutlet connection
55
Auflageabsatzsupporting shoulder
66
Auslaufende (rund oder sternenförmig)Expiring (round or star shaped)

Claims (8)

  1. Method for producing a multiwell filtration plate, characterized by producing the sample holder part (1) and the outlet part (2) comprising the outlet connecting piece (4) via hot channel technology, directly aligned with each other, with a maximum tolerance of 1/1000mm, and by connecting them at the depressions of the outlet connecting pieces via hydraulic compression so that a firm and tight connection is formed, further characterized by forming sharp, inner compression edges by using suitable tools, so that when a filter insert (3) is placed between sample holder part (1) and outlet part (2) during compression, filters are punched out into the corresponding depressions, which are pressed against the outlet piece (2) by the sample holder part (1) and are fixed on the supporting shoulder (5).
  2. Multiwell filtration plate produced by a method according to claim 1.
  3. Multiwell filtration plate according to claim 2, further characterized by a sample holder part (1) that is firmly and tightly connected to the outlet part (2), that comprises an outlet connecting piece (4) for each well and that has a length of 10-15mm in the case of a 96 well plate, wherein a supporting shoulder (5) carrying a filter insert (3) is present at the connection of sample holder part and outlet part.
  4. Multiwell filtration plate according to claims 2-3, characterized by an outlet connecting piece (4) that is round or by an outlet connecting piece bottom portion (6) that is star-shaped.
  5. Multiwell filtration plate according to claim 4, characterized by a star-shaped outlet connecting piece bottom portion (6) that has eight openings.
  6. Multiwell filtration plate according to claims 2-5, characterized by being a 96 well plate, wherein the outlet connecting piece (4) has a length of 10-15mm, preferably 12mm.
  7. Multiwell filtration plate according to claims 2, 4 or 5, characterized by being a 384 well plate.
  8. Use of a multiwell filtration plate according to claims 2-7 for high-throughput applications related to nucleic acids.
EP01964906A 2000-08-25 2001-08-22 Multiwell filtration plate and method for producing the same Expired - Lifetime EP1313562B1 (en)

Applications Claiming Priority (3)

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DE10041825 2000-08-02
DE10041825A DE10041825A1 (en) 2000-08-25 2000-08-25 Multiwell filtration plate and process for its manufacture
PCT/DE2001/003137 WO2002016035A1 (en) 2000-08-25 2001-08-22 Multiwell filtration plate and method for producing the same

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EP1313562A1 EP1313562A1 (en) 2003-05-28
EP1313562B1 true EP1313562B1 (en) 2006-10-25

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US (1) US6830732B1 (en)
EP (1) EP1313562B1 (en)
JP (1) JP2004506918A (en)
CN (1) CN1471432A (en)
AT (1) ATE343426T1 (en)
AU (1) AU2001285706A1 (en)
DE (2) DE10041825A1 (en)
DK (1) DK1313562T3 (en)
ES (1) ES2275719T3 (en)
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ES2275719T3 (en) 2007-06-16
CN1471432A (en) 2004-01-28
ATE343426T1 (en) 2006-11-15
DK1313562T3 (en) 2007-02-26
EP1313562A1 (en) 2003-05-28
AU2001285706A1 (en) 2002-03-04
DE10041825A1 (en) 2002-03-07
JP2004506918A (en) 2004-03-04
US6830732B1 (en) 2004-12-14
WO2002016035A1 (en) 2002-02-28

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