EP1257266A1 - Intravenöse gabe von carnitin zur behandlung von periodisch dialysierten patienten mit chronischer niereninsuffizienz - Google Patents
Intravenöse gabe von carnitin zur behandlung von periodisch dialysierten patienten mit chronischer niereninsuffizienzInfo
- Publication number
- EP1257266A1 EP1257266A1 EP01902636A EP01902636A EP1257266A1 EP 1257266 A1 EP1257266 A1 EP 1257266A1 EP 01902636 A EP01902636 A EP 01902636A EP 01902636 A EP01902636 A EP 01902636A EP 1257266 A1 EP1257266 A1 EP 1257266A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- carnitine
- dialysis
- treatment
- use according
- patients
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/205—Amine addition salts of organic acids; Inner quaternary ammonium salts, e.g. betaine, carnitine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/08—Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock
Definitions
- the present invention relates to an improved therapeutic method for the treatment of chronic uraemic patients undergoing periodical dialysis.
- post-dialytic syndrome A method for treating the post-dialytic syndrome is disclosed in
- US 4,272,549 teaches to treat the "post-dialytic syndrome" by compensating the loss of carnitine occurring during dialytic session.
- US 4,272,549 claims a method for alleviating asthenia and muscle weakness in a chronic uraemic patient under regular dialysis treatment, which comprises submitting said patient with a polysaline dialytic solution which contains a quantity of carnitine (intended as L-carnitine throughout the present application), or a pharmaceutically acceptable salt thereof, sufficient to render the molar concentration of carnitine in said solution at least equal to the molar concentration of the plasma carnitine of the patient under dialytic treatment.
- Preferred embodiments of the invention provides that the concentration of carnitine in the dialytic solution is substantially equimolar to the concentration of carnitine in the patient's plasma, but a certain excess of carnitine is also provided, for example between 50 and 100 ⁇ mole per liter of solution.
- An embodiment of the invention provides the administration of from 3 to 6 grams of carnitine or an equivalent amount of a pharmaceutically acceptable salt thereof.
- the preferred embodiment of the invention provides the oral administration of carnitine, in particular on days between haemodialysis of from 3 to 6 grams of carnitine per day.
- the oral treatment is coupled with a rather complex treatment with carnitine during the dialytic session, which comprises the administration of carnitine by slow infusion.
- carnitine On the days of haemodialytic session, carnitine may also be administered partly by the oral route and partly by slow infusion. In this case, the overall quantity of carnitine administered shall not exceed approximately 10 g.
- “Slow infusion” stands for an infusion in which the solution containing carnitine, or any of its pharmaceutically acceptable salts, is administered at the rate of 20 to 40 drops per minute. Particularly favourable therapeutic results were achieved by a method in which carnitine is administered by the oral route to the patient under haemodialytic treatment only on those days during which the patient is not submitted to a dialytic session, while during the actual dialytic session, a dialysing liquid containing carnitine is used.
- Such preferred therapeutic method for the treatment of chronic uraemic patients undergoing haemodialysis included more particularly the following steps: 1) on the days between one haemodialytic session and the next, administration by the oral route to these patients of 3 to 6 g per day of carnitine or any of its pharmaceutically acceptable salts;
- tissue carnitine depletion which is the long-term consequence of repeated losses of carnitine the patienl undergoes during the successive dialytic sessions he is submitted to over a prolonged period of time.
- the solution for the haemodialysis be equimolar in carnitine with respect to the blood of the patient under dialytic treatment, it is preferred to operate with a slightly more concentrated solution.
- the haemodialysis solution contains 50 to 100, preferably 60 - 80 ⁇ moles/ litre of carnitine or of any of its pharmaceutically acceptable salts.
- carnitine may also be administered partly by the oral route and partly by slow infusion. In this case, the overall quantity of carnitine administered shall not exceed approximately 10 g.
- the method for the treatment of chronic uraemic patients undergoing periodical dialysis is useful for preventing and/ or treating carnitine deficiency in patients with end stage renal disease who are undergoing dialysis.
- the method according to the present invention comprises administering an effective dose of carnitine intravenously into the venous return line after each dialysis session.
- the method according to the present invention provides the surprising improvement with respect to the method disclosed in US 4,272,549 to eliminate the need of the oral treatment, without affecting the maintenance of the correction of carnitine deficiency obtained by the administration of carnitine through intravenous route.
- Figure 1 illustrates the treatment schedule, where the letters A-F denote the heart effluent sampling times for the measurement of metabolites.
- Figure 2 shows the effect of carnitine (A) and carnitine fumarate (B) on creatine phosphate and ATP.
- Figure 3 compares lactate (A) with succinate (B) released by the heart, as measured in the effluent.
- Figure 4 illustrates the release of malate.
- Figure 5 illustrates the release of LDH.
- Figure 6 illustrates the production of lactate.
- the preferred starting dose is 10-20 mg/kg dry body weight as a slow 2-3 minute bolus injection into the venous return line after each dialysis session.
- Initiation of the therapy may be prompted by through (pre- dialysis) plasma carnitine concentrations that are below normal (40- 50 umol/L).
- Dose adjustments should be guided by through (pre- dialysis) carnitine concentrations, and downward dose adjustments (for example to 5 mg/kg after dialysis) may be made as early as the third or fourth week of therapy.
- Carnitine can be administered as inner salt or in any pharmaceutically acceptable salts thereof.
- the invention is given in detailed manner as to put it into practice.
- the active ingredient is mentioned as "carnitine or any of its pharmaceutically acceptable salts" (see for instance US 4272549, column 4, line 16), thus clearly teaching to the skilled person that any of the pharmaceutically acceptable salts will do in the invention therein disclosed.
- any of the pharmaceutically acceptable salts of carnitine will do. But, in a particular embodiment of the invention, the skilled person might have to face a problem with some patients. During the dialytic session, some patients are affected by hypervolemic heart, and this can give a severe outcome as heart failure. Moreover, a number of patients undergoing hemodialysis are affected by diabetes.
- fumarate of L-carnitine exerts a surprising beneficial effect on heart. Moreover, due to its physiologic role, fumarate may have beneficial effects in diabetic patients. Accordingly, a particular embodiment of the present invention relates to the method above disclosed, wherein fumarate is the pharmaceutically acceptable salt of L-carnitine.
- Suitable formulations of carnitine, or a pharmaceutically acceptable salt thereof are in the form of injectable compositions, for example comprising an equivalent amount of carnitine of 200 mg per 1 mL. A 2.5 or a 5 mL single dose ampoule may be convenient.
- a pharmaceutically acceptable salt of L-carnitine such as fumarate
- the amount of active ingredient shall be calculated as to give an equivalent amount of L-carnitine as above specified.
- the treatment was repeated twice a week every 44 hours, then after 68 hours. This treatment was continued for 3-4 weeks, monitoring pre-dialytic levels of carnitine.
- a maintenance method of treatment is provided, giving, as a preferred example, a dose of 5 mg/kg of carnitine.
- X shows a 4-hours dialytic session and the carnitine intravenous administration according to the present invention at the end of the session. 44 hours occur between two subsequent carnitine administrations from Monday to Friday and 68 hours occur between two subsequent carnitine administrations from Friday to Monday.
- the low-pressure or low-flow ischemia model was used, which is a model recognised as valid for cardiac ischemia (Bolukoglu, H. et al. Am. J. Physiol. 1996: 270; H817-26).
- the treatment schedule is illustrated in Figure 1., in which the letters A-F denote the heart effluent sampling times for the measurement of metabolites.
- the hearts are removed from the animals and mounted on a Langerdorff appliance.
- the perfusion medium replacing the blood was a Krebs-Heinsleit standard bicarbonate buffer containing glucose 12 mM as energy source for cardiac metabolism.
- ischemia was induced by reducing the perfusion pressure of the heart to 25 cm of water, thus reducing coronary flow from approximately 2 ml/min to approximately 0.3 ml/min. Reduction of the perfusion pressure gives rise to ischemia, since the heart will pump the fluid in the low-perfusion area rather than via the coronary bloodstream, supplying the flow to the heart.
- This control model was compared with hearts perfused with L- carnitine 10 mM or L-carnitine fumarate 10 mM.
- Cardiac function was tested in three different ways.
- the NRM 31 P signal was monitored in real time.
- This signal provides the best indication of the energy status of the heart.
- the haemodynamics of the heart was measured by means of a pressure transducer mounted to measure the perfusion pressure.
- the haemodynamic measurements include heart rate, relative dP/dt (measurement of the contraction force of the heart) and the cardiac contraction amplitude. Coronary flow was also measured as an indicator of the heart's ability to provide oxygen and energy for its own metabolism.
- the metabolites and the enzyme LDH released by the heart were analysed in the effluent.
- the release of LDH indicates damage to cardiac tissue.
- the release of metabolites by the heart was tested by means of mass spectrometry coupled with gas chromatography.
- FIG. 2 illustrates the effect of carnitine (A) and carnitine fumarate (B) on creatine phosphate and ATP.
- the data were evaluated after 40 minutes of ischemia.
- CP indicates creatine phosphate and ⁇ , ⁇ and ⁇ denote the phosphate peaks of ATP; as can be seen in part (A) of the figure, the ATP peaks are lacking in the absence of fumarate.
- Figure 3 shows the comparison between lactate (A) and succinate (B) released by the heart, as measured in the effluent.
- the lactate reduction indicates the favourable effect of carnitine fumarate.
- the low amount of succinate as compared to lactate indicates that the generation of ATP as a result of the reduction of fumarate to succinate is not the main source of anaerobic ATP.
- Figure 4 illustrates the release of malate.
- the greater malate levels in the treated heart indicate that fumarate enters the cardiac mitochondrion and is metabolised in the TCA cycle.
- Figure 5 illustrates the release of LDH.
- the greater LDH levels in controls indicate that carnitine fumarate affords protection against ischemic damage.
- Figure 6 illustrates lactate production
Landscapes
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US17662900P | 2000-01-19 | 2000-01-19 | |
US176629P | 2000-01-19 | ||
US18632800P | 2000-03-02 | 2000-03-02 | |
US186328P | 2000-03-02 | ||
PCT/IT2001/000012 WO2001052836A1 (en) | 2000-01-19 | 2001-01-15 | Intravenous carnitine for the treatment of chronic uraemic patients undergoing periodical dialysis |
Publications (1)
Publication Number | Publication Date |
---|---|
EP1257266A1 true EP1257266A1 (de) | 2002-11-20 |
Family
ID=26872436
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP01902636A Withdrawn EP1257266A1 (de) | 2000-01-19 | 2001-01-15 | Intravenöse gabe von carnitin zur behandlung von periodisch dialysierten patienten mit chronischer niereninsuffizienz |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1257266A1 (de) |
AU (1) | AU2001230502A1 (de) |
CA (1) | CA2381187C (de) |
WO (1) | WO2001052836A1 (de) |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT1156741B (it) * | 1978-05-15 | 1987-02-04 | Sigma Tau Ind Farmaceuti | Applicazione terapeutica della carnitina e di alcuni derivati acilati della carnitina nell'emodialisi |
AU8898898A (en) * | 1997-08-07 | 1999-03-01 | Ajay Gupta | Dialysis solutions containing water soluble vitamins and nutrients |
-
2001
- 2001-01-15 CA CA002381187A patent/CA2381187C/en not_active Expired - Fee Related
- 2001-01-15 WO PCT/IT2001/000012 patent/WO2001052836A1/en active Application Filing
- 2001-01-15 EP EP01902636A patent/EP1257266A1/de not_active Withdrawn
- 2001-01-15 AU AU2001230502A patent/AU2001230502A1/en not_active Abandoned
Non-Patent Citations (1)
Title |
---|
See references of WO0152836A1 * |
Also Published As
Publication number | Publication date |
---|---|
CA2381187A1 (en) | 2001-07-26 |
WO2001052836A1 (en) | 2001-07-26 |
CA2381187C (en) | 2009-06-02 |
AU2001230502A1 (en) | 2001-07-31 |
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