EP1222251A4 - Ex vivo gewinnung von funktionellen osteoklasten aus dem knochenmark in einem dreidimensionalen biorektor - Google Patents

Ex vivo gewinnung von funktionellen osteoklasten aus dem knochenmark in einem dreidimensionalen biorektor

Info

Publication number
EP1222251A4
EP1222251A4 EP00955457A EP00955457A EP1222251A4 EP 1222251 A4 EP1222251 A4 EP 1222251A4 EP 00955457 A EP00955457 A EP 00955457A EP 00955457 A EP00955457 A EP 00955457A EP 1222251 A4 EP1222251 A4 EP 1222251A4
Authority
EP
European Patent Office
Prior art keywords
bone marrow
vivo generation
functional osteoclasts
dimensional bioreactor
bioreactor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00955457A
Other languages
English (en)
French (fr)
Other versions
EP1222251A1 (de
Inventor
J H David Wu
Athanassios Mantalaris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Rochester
Original Assignee
University of Rochester
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Rochester filed Critical University of Rochester
Publication of EP1222251A1 publication Critical patent/EP1222251A1/de
Publication of EP1222251A4 publication Critical patent/EP1222251A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0643—Osteoclasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/12—Drugs for disorders of the metabolism for electrolyte homeostasis
    • A61P3/14—Drugs for disorders of the metabolism for electrolyte homeostasis for calcium homeostasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/37—Parathyroid hormone [PTH]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/76—Agarose, agar-agar
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/78—Cellulose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Diabetes (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Endocrinology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Obesity (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP00955457A 1999-08-13 2000-08-11 Ex vivo gewinnung von funktionellen osteoklasten aus dem knochenmark in einem dreidimensionalen biorektor Withdrawn EP1222251A4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14881499P 1999-08-13 1999-08-13
US148814P 1999-08-13
PCT/US2000/022040 WO2001012785A1 (en) 1999-08-13 2000-08-11 Ex vivo generation of functional osteoclasts from bone marrow in a three-dimensional bioreactor

Publications (2)

Publication Number Publication Date
EP1222251A1 EP1222251A1 (de) 2002-07-17
EP1222251A4 true EP1222251A4 (de) 2004-07-07

Family

ID=22527509

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00955457A Withdrawn EP1222251A4 (de) 1999-08-13 2000-08-11 Ex vivo gewinnung von funktionellen osteoklasten aus dem knochenmark in einem dreidimensionalen biorektor

Country Status (4)

Country Link
EP (1) EP1222251A4 (de)
JP (1) JP2003507035A (de)
AU (1) AU6766100A (de)
WO (1) WO2001012785A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107246988A (zh) * 2017-06-06 2017-10-13 珠海贝索生物技术有限公司 一种抗酸性菌染色方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1259634A4 (de) 2000-03-02 2004-07-07 Univ Rochester Ex vivo erstellung funktioneller leukämiezellen in einem dreidimensionalen bioreaktor
EP1545603B1 (de) 2002-07-25 2015-09-16 The General Hospital Corporation Parathyreoidhormon-rezeptor-aktivierung und expansion von hämatopoetischen vorläuferzellen
US7635477B2 (en) 2002-07-25 2009-12-22 The General Hospital Corporation Parathyroid hormone receptor activation and stem and progenitor cell expansion
US20050031598A1 (en) * 2002-12-10 2005-02-10 Shulamit Levenberg Engineering three-dimensional tissue structures using differentiating embryonic stem cells
DE102008036060A1 (de) * 2008-08-04 2010-02-25 Technische Universität Dresden Zellbasiertes Verfahren und Mittel zum Knochenaufbau

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5719058A (en) * 1995-04-10 1998-02-17 Merck & Co., Inc. Method for producing a highly enriched population of osteoclast cells

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DE BRUIJN J D ET AL: "Osteoclastic resorption of calcium phosphates is potentiated in postosteogenic culture conditions", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, vol. 28, no. 1, 1994, pages 105 - 112, XP009024411, ISSN: 0021-9304 *
LACEY D L ET AL: "OSTEOPROTEGERIN LIGAND IS A CYTOKINE THAT REGULATES OSTEOCLAST DIFFERENTIATION AND ACTIVATION", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 93, 17 April 1998 (1998-04-17), pages 165 - 176,1, XP002934582, ISSN: 0092-8674 *
MANTALARIS A ET AL: "ENGINEERING A HUMAN BONE MARROW MODEL: A CASE STUDY ON EX VIVO ERYTHROPOIESIS", BIOTECHNOLOGY PROGRESS, XX, XX, vol. 14, no. 1, 1998, pages 126 - 133, XP002944957, ISSN: 8756-7938 *
SATO T ET AL: "THE MIGRATION OF PURIFIED OSTEOCLASTS THROUGH COLLAGEN IS INHIBITEDBY MATRIX METALLOPROTEINASE INHIBITORS", JOURNAL OF BONE AND MINERAL RESEARCH, NEW YORK, NY, US, vol. 13, no. 1, January 1998 (1998-01-01), pages 59 - 66, XP000992863, ISSN: 0884-0431 *
See also references of WO0112785A1 *
UDAGAWA N ET AL: "ORIGIN OF OSTEOCLASTS MATURE MONOCYTES AND MACROPHAGES ARE CAPABLE OF DIFFERENTIATING INTO OSTEOCLASTS UNDER A SUITABLE MICROENVIRONMENT PREPARED BY BONE MARROW-DERIVED STROMAL CELLS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 87, no. 18, 1990, pages 7260 - 7264, XP002199542, ISSN: 0027-8424 *
WANG T-Y BRENNAN J K ET AL: "MULTILINEAL HEMATOPOIESIS IN A THREE-DIMENSIONAL MURINE LONG-TERM BONE MARROW CULTURE", EXPERIMENTAL HEMATOLOGY, NEW YORK, NY, US, vol. 23, 1995, pages 26 - 32, XP002944956, ISSN: 0301-472X *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107246988A (zh) * 2017-06-06 2017-10-13 珠海贝索生物技术有限公司 一种抗酸性菌染色方法

Also Published As

Publication number Publication date
JP2003507035A (ja) 2003-02-25
EP1222251A1 (de) 2002-07-17
WO2001012785A1 (en) 2001-02-22
AU6766100A (en) 2001-03-13

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