EP1201759A4 - Trap vector and gene trapping method by using the same - Google Patents

Trap vector and gene trapping method by using the same

Info

Publication number
EP1201759A4
EP1201759A4 EP00922969A EP00922969A EP1201759A4 EP 1201759 A4 EP1201759 A4 EP 1201759A4 EP 00922969 A EP00922969 A EP 00922969A EP 00922969 A EP00922969 A EP 00922969A EP 1201759 A4 EP1201759 A4 EP 1201759A4
Authority
EP
European Patent Office
Prior art keywords
sequence
inverted repeat
loxp
trap vector
same
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP00922969A
Other languages
German (de)
French (fr)
Other versions
EP1201759B1 (en
EP1201759A1 (en
Inventor
K Yamamura
Kimi Araki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transgenic Inc
Original Assignee
Transgenic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgenic Inc filed Critical Transgenic Inc
Priority to EP08008313A priority Critical patent/EP2031068A1/en
Publication of EP1201759A1 publication Critical patent/EP1201759A1/en
Publication of EP1201759A4 publication Critical patent/EP1201759A4/en
Application granted granted Critical
Publication of EP1201759B1 publication Critical patent/EP1201759B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Physical Deposition Of Substances That Are Components Of Semiconductor Devices (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A vector generated from recombination between; a trap vector comprising a loxP sequence and a mutant loxP sequence, wherein the loxP sequence comprises in sequential order inverted repeat sequence 1, a spacer sequence, and inverted repeat sequence 2, and the mutant loxP sequence comprises a sequence of which a part of said inverted repeat sequence 1 of loxP is mutated and (b) a trap vector comprising a mutant loxP sequence, wherein the mutant loxP sequence comprises in sequential order inverted repeat sequence 1, a spacer sequence, and inverted repeat sequence 2 wherein a part of said inverted repeat sequence 2 is mutated, or (c) a trap vector comprising a loxP sequence and a mutant loxP sequence, wherein the loxP sequence comprises in sequential order inverted repeat sequence 1, a spacer sequence, and inverted repeat sequence 2; and the mutant loxP sequence comprises a sequence of which a part of said inverted repeat sequence 2 of loxP is mutated and (d) a trap vector comprising a mutant loxP sequence, wherein the mutant loxP sequence comprises in sequential order inverted repeat sequence I, a spacer sequence, and inverted repeat sequence 2 wherein a part of said inverted repeat sequence 1 is mutated.
EP00922969A 1999-07-14 2000-05-02 Trap vector and gene trapping method by using the same Expired - Lifetime EP1201759B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP08008313A EP2031068A1 (en) 1999-07-14 2000-05-02 Trap vector and gene trapping method by using the same

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP20099799 1999-07-14
JP20099799 1999-07-14
PCT/JP2000/002916 WO2001005987A1 (en) 1999-07-14 2000-05-02 Trap vector and gene trapping method by using the same

Related Child Applications (3)

Application Number Title Priority Date Filing Date
EP08008313A Division EP2031068A1 (en) 1999-07-14 2000-05-02 Trap vector and gene trapping method by using the same
EP08005752.4 Division-Into 2008-03-27
EP08008313.2 Division-Into 2008-05-01

Publications (3)

Publication Number Publication Date
EP1201759A1 EP1201759A1 (en) 2002-05-02
EP1201759A4 true EP1201759A4 (en) 2002-07-31
EP1201759B1 EP1201759B1 (en) 2010-03-10

Family

ID=16433801

Family Applications (2)

Application Number Title Priority Date Filing Date
EP00922969A Expired - Lifetime EP1201759B1 (en) 1999-07-14 2000-05-02 Trap vector and gene trapping method by using the same
EP08008313A Withdrawn EP2031068A1 (en) 1999-07-14 2000-05-02 Trap vector and gene trapping method by using the same

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP08008313A Withdrawn EP2031068A1 (en) 1999-07-14 2000-05-02 Trap vector and gene trapping method by using the same

Country Status (11)

Country Link
US (3) US7312075B1 (en)
EP (2) EP1201759B1 (en)
JP (2) JP4664554B2 (en)
KR (1) KR20020057943A (en)
CN (3) CN101210254A (en)
AT (1) ATE460492T1 (en)
AU (1) AU778719B2 (en)
CA (1) CA2379055A1 (en)
DE (1) DE60043985D1 (en)
HK (1) HK1048830A1 (en)
WO (1) WO2001005987A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7074611B2 (en) * 2001-04-27 2006-07-11 Gie-Cerbn, Centre Europeen De Recherche En Biologie Et En Medecine (Gie) Method for the stable inversion of DNA sequence by site-specific recombination and DNA vectors and transgenic cells thereof
DE10140030C1 (en) * 2001-08-16 2002-12-19 Gsf Forschungszentrum Umwelt Method for performing multiple recombination events in a genetic system, useful e.g. for removing antibiotic resistance genes, uses mutant recombinase recognition sites
CN100370024C (en) * 2003-05-12 2008-02-20 上海南方模式生物科技发展有限公司 Method of establishing mouse genome mutant embryonic stem cell library
CN100427591C (en) * 2003-08-06 2008-10-22 上海南方模式生物科技发展有限公司 Method for screening and establishing mouse-development-related gene mutation ES cell bank
CN100427592C (en) * 2003-08-06 2008-10-22 上海南方模式生物科技发展有限公司 Method for establishing mouse full-genome mutoation ES cell bank
JP5481661B2 (en) * 2008-10-23 2014-04-23 学校法人福岡大学 Mutation gene production method
CA2868292A1 (en) 2012-03-27 2013-10-03 National University Corporation Kumamoto University Immunodeficient mouse with humanized liver
CN107429261A (en) 2014-12-15 2017-12-01 应用干细胞有限公司 Produce single or multiplexed reporters method and compositions rapidly in cell
CN107729300B (en) * 2017-09-18 2021-12-24 百度在线网络技术（北京）有限公司 Text similarity processing method, device and equipment and computer storage medium
CN108004264B (en) * 2017-12-14 2020-09-08 华中科技大学 Pichia pastoris gene knockout and resistance gene recovery vector, construction method and application
CN110751982B (en) * 2018-07-04 2023-11-10 广州赛业百沐生物科技有限公司 Intelligent parallelization knockout strategy screening method and system
CN117051039B (en) * 2023-07-19 2024-05-24 中国科学院水生生物研究所 Plasmid containing LoxP locus at two ends and expressing GFP protein, construction method and application thereof

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO1999002719A1 (en) * 1997-07-11 1999-01-21 The University Of British Columbia Complementation trap

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US4237224A (en) 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
US4468464A (en) 1974-11-04 1984-08-28 The Board Of Trustees Of The Leland Stanford Junior University Biologically functional molecular chimeras
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
US4736866B1 (en) 1984-06-22 1988-04-12 Transgenic non-human mammals
JPS6242239Y2 (en) * 1984-10-15 1987-10-29
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US5378618A (en) 1988-04-15 1995-01-03 E. I. Du Pont De Nemours And Company Vitro headful packaging system for cloning DNA fragments as large as 95kb
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5658772A (en) 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
US5300431A (en) 1991-02-26 1994-04-05 E. I. Du Pont De Nemours And Company Positive selection vector for the bacteriophage P1 cloning system
WO1993004169A1 (en) 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
GB9308271D0 (en) 1993-04-21 1993-06-02 Univ Edinburgh Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method
US5928914A (en) * 1996-06-14 1999-07-27 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Methods and compositions for transforming cells
US6139833A (en) 1997-08-08 2000-10-31 Lexicon Genetics Incorporated Targeted gene discovery
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US6037125A (en) 1996-11-05 2000-03-14 Lexicon Genetics Incorporated Disruption of the mammalian RAD51 protein and disruption of proteins that associate with mammalian RAD51 for hindering cell proliferation and/or viability of proliferating cells
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WO1999002719A1 (en) * 1997-07-11 1999-01-21 The University Of British Columbia Complementation trap

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ALBERT H ET AL: "Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome", PLANT JOURNAL, BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, GB, vol. 7, no. 4, 1995, pages 649 - 659, XP002097329, ISSN: 0960-7412 *
ARAKI K ET AL: "EXCHANGEABLE GENE TRAP USING THE CRE/MUTATED LOX SYSTEM", MOLECULAR AND CELLULAR BIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 45, no. 5, July 1999 (1999-07-01), pages 737 - 750, XP000877476, ISSN: 0270-7306 *
KUHN R ET AL: "Advances in gene targeting methods", CURRENT OPINION IN IMMUNOLOGY, CURRENT BIOLOGY LTD, XX, vol. 9, no. 2, April 1997 (1997-04-01), pages 183 - 188, XP004327249, ISSN: 0952-7915 *
ZAMBROWICZ AND G A FRIEDRICH B P: "Comprehensive mammalian genetics: history and fututre prospects of gene trapping in the mouse", INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, UNIVERSITY OF THE BASQUE COUNTRY PRES, LEIOA, ES, vol. 42, 1998, pages 1025 - 1036, XP002111634, ISSN: 0214-6282 *

Also Published As

Publication number Publication date
WO2001005987A1 (en) 2001-01-25
CA2379055A1 (en) 2001-01-25
EP1201759B1 (en) 2010-03-10
KR20020057943A (en) 2002-07-12
US20090202988A1 (en) 2009-08-13
EP2031068A1 (en) 2009-03-04
AU4317600A (en) 2001-02-05
CN101210254A (en) 2008-07-02
ATE460492T1 (en) 2010-03-15
CN1721529B (en) 2013-03-13
CN1375012A (en) 2002-10-16
EP1201759A1 (en) 2002-05-02
US8722408B2 (en) 2014-05-13
CN1375012B (en) 2010-05-26
DE60043985D1 (en) 2010-04-22
CN1721529A (en) 2006-01-18
US20110117642A1 (en) 2011-05-19
US7312075B1 (en) 2007-12-25
AU778719B2 (en) 2004-12-16
JP2011045380A (en) 2011-03-10
JP4664554B2 (en) 2011-04-06
HK1048830A1 (en) 2003-04-17

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