WO2000009755A3 - Domain specific gene evolution - Google Patents

Domain specific gene evolution Download PDF

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Publication number
WO2000009755A3
WO2000009755A3 PCT/US1999/018393 US9918393W WO0009755A3 WO 2000009755 A3 WO2000009755 A3 WO 2000009755A3 US 9918393 W US9918393 W US 9918393W WO 0009755 A3 WO0009755 A3 WO 0009755A3
Authority
WO
WIPO (PCT)
Prior art keywords
specific gene
domain specific
gene evolution
evolution
recombinase
Prior art date
Application number
PCT/US1999/018393
Other languages
French (fr)
Other versions
WO2000009755A2 (en
WO2000009755A9 (en
Inventor
David Zarling
Gurucharan Reddy
Sushma Pati
Original Assignee
Pangene Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/133,934 external-priority patent/US6074853A/en
Application filed by Pangene Corporation filed Critical Pangene Corporation
Priority to JP2000565189A priority Critical patent/JP2002522096A/en
Priority to CA002339420A priority patent/CA2339420A1/en
Priority to AU57748/99A priority patent/AU772879B2/en
Priority to EP99945053A priority patent/EP1105472A2/en
Publication of WO2000009755A2 publication Critical patent/WO2000009755A2/en
Publication of WO2000009755A3 publication Critical patent/WO2000009755A3/en
Publication of WO2000009755A9 publication Critical patent/WO2000009755A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms

Abstract

Compositions and methods are provided for rapidly evolving specific protein domains employing a recombinase and recombination intermediates.
PCT/US1999/018393 1998-08-12 1999-08-12 Domain specific gene evolution WO2000009755A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000565189A JP2002522096A (en) 1998-08-12 1999-08-12 Development of domain-specific genes
CA002339420A CA2339420A1 (en) 1998-08-12 1999-08-12 Domain specific gene evolution
AU57748/99A AU772879B2 (en) 1998-08-12 1999-08-12 Domain specific gene evolution
EP99945053A EP1105472A2 (en) 1998-08-12 1999-08-12 Domain specific gene evolution

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US9633098P 1998-08-12 1998-08-12
US09/133,934 1998-08-14
US60/096,330 1998-08-14
US09/133,934 US6074853A (en) 1997-03-21 1998-08-14 Sequence alterations using homologous recombination

Publications (3)

Publication Number Publication Date
WO2000009755A2 WO2000009755A2 (en) 2000-02-24
WO2000009755A3 true WO2000009755A3 (en) 2000-06-08
WO2000009755A9 WO2000009755A9 (en) 2000-08-03

Family

ID=26791582

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/018393 WO2000009755A2 (en) 1998-08-12 1999-08-12 Domain specific gene evolution

Country Status (5)

Country Link
EP (1) EP1105472A2 (en)
JP (1) JP2002522096A (en)
AU (1) AU772879B2 (en)
CA (1) CA2339420A1 (en)
WO (1) WO2000009755A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001066717A2 (en) * 2000-03-03 2001-09-13 The University Of Utah Gene targeting method
EP1152058A1 (en) 2000-05-03 2001-11-07 Institut Curie Methods and compositions for effecting homologous recombination
US20020045175A1 (en) * 2000-05-23 2002-04-18 Zhen-Gang Wang Gene recombination and hybrid protein development
AU2001266978A1 (en) * 2000-06-14 2001-12-24 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
JP4616988B2 (en) * 2000-12-20 2011-01-19 株式会社アイシン・コスモス研究所 DNA polymorphism detection method applying triple-stranded DNA formation technology
US8106255B2 (en) 2002-01-23 2012-01-31 Dana Carroll Targeted chromosomal mutagenasis using zinc finger nucleases
GB0218803D0 (en) * 2002-08-13 2002-09-18 Nl Kanker I Targeted gene modification by single-standed DNA oligonucleotides
CA2497913C (en) 2002-09-05 2014-06-03 California Institute Of Technology Use of chimeric nucleases to stimulate gene targeting
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US20120196370A1 (en) 2010-12-03 2012-08-02 Fyodor Urnov Methods and compositions for targeted genomic deletion
US7972854B2 (en) 2004-02-05 2011-07-05 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US11136609B2 (en) * 2015-11-09 2021-10-05 National University Corporation Kyoto Institute Of Technology Separating agent for human serum-derived IgG polyclonal antibodies, and method for separating human serum-derived IgG polyclonal antibodies using same

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993022443A1 (en) * 1992-04-24 1993-11-11 Sri International In vivo homologous sequence targeting in eukaryotic cells
EP0718404A2 (en) * 1994-11-22 1996-06-26 Takara Shuzo Co. Ltd. Method for site-directed mutagenesis
US5731411A (en) * 1993-07-12 1998-03-24 The United States Of America As Represented By The Department Of Health And Human Services Promotion of homologous DNA pairing by RecA-derived peptides
WO1998042727A1 (en) * 1997-03-21 1998-10-01 Sri International Sequence alterations using homologous recombination

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6262098A (en) * 1997-02-03 1998-08-25 Vitagen Inc. Human regeneration-associated serpin-1 (rasp-1) and methods of use

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993022443A1 (en) * 1992-04-24 1993-11-11 Sri International In vivo homologous sequence targeting in eukaryotic cells
US5731411A (en) * 1993-07-12 1998-03-24 The United States Of America As Represented By The Department Of Health And Human Services Promotion of homologous DNA pairing by RecA-derived peptides
EP0718404A2 (en) * 1994-11-22 1996-06-26 Takara Shuzo Co. Ltd. Method for site-directed mutagenesis
WO1998042727A1 (en) * 1997-03-21 1998-10-01 Sri International Sequence alterations using homologous recombination

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HASTY P ET AL: "THE LENGTH OF HOMOLOGY REQUIRED FOR GENE TARGETING IN EMBRYONIC STEM CELLS", MOLECULAR AND CELLULAR BIOLOGY,US,WASHINGTON, DC, vol. 11, no. 11, 1 November 1991 (1991-11-01), pages 5586 - 5591, XP002052222, ISSN: 0270-7306 *
KOWALCZYKOWSKI ET AL: "In vitro reconstitution of homologous recombination reactions", EXPERIENTIA,CH,BIRKHAUSER VERLAG. BASEL, vol. 50, no. 50, 1994, pages 204 - 215-215, XP002109111, ISSN: 0014-4754 *

Also Published As

Publication number Publication date
WO2000009755A2 (en) 2000-02-24
WO2000009755A9 (en) 2000-08-03
AU5774899A (en) 2000-03-06
AU772879B2 (en) 2004-05-13
EP1105472A2 (en) 2001-06-13
CA2339420A1 (en) 2000-02-24
JP2002522096A (en) 2002-07-23

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