EP1190246A2 - Appareils et procedes d'evaluation du transport cellulaire pour la reserche, la decouverte et les tests d'un medicament, les diagnostics cliniques et l'elaboration d'une therapie - Google Patents

Appareils et procedes d'evaluation du transport cellulaire pour la reserche, la decouverte et les tests d'un medicament, les diagnostics cliniques et l'elaboration d'une therapie

Info

Publication number
EP1190246A2
EP1190246A2 EP00937916A EP00937916A EP1190246A2 EP 1190246 A2 EP1190246 A2 EP 1190246A2 EP 00937916 A EP00937916 A EP 00937916A EP 00937916 A EP00937916 A EP 00937916A EP 1190246 A2 EP1190246 A2 EP 1190246A2
Authority
EP
European Patent Office
Prior art keywords
cell
cells
medium
efflux
ofthe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00937916A
Other languages
German (de)
English (en)
Inventor
Miklos Gratzl
Hongwen Lu
Takashi Matsumoto
Chen Yi
Gary R. Bright
Rohit Kashyap
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Case Western Reserve University
Original Assignee
Case Western Reserve University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Case Western Reserve University filed Critical Case Western Reserve University
Publication of EP1190246A2 publication Critical patent/EP1190246A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp

Abstract

L'invention concerne un procédé de mesure des sorties/entrées d'un médicament (26), tel que la doxuribicine, dans une cellule (14, 52), telle que la cellule cancéreuse humaine ou une autre cellule biologique. Ce procédé consiste à introduire le médicament dans la cellule, par exemple par immersion de la cellule dans un milieu de culture contenant le médicament, ou par injection directe dans la cellule. La cellule contenant le médicament est alors immergée dans un milieu (28) liquide exempt de médicament et les sorties/entrées du médicament dans la cellule sont mesurées à l'aide d'un équipement (60) électrochimique. En ce qui concerne la détection des sorties depuis des petites populations de cellules, ou depuis une cellule unique, l'intensité du signal peut être augmentée par concentration préalable du médicament sur une électrode (20, 50) de travail et par augmentation de la concentration d'oxygène du milieu entourant la cellule. Une cellule ou une petit groupe de cellules (52) à étudier peut être placé, de manière espacée, sur un substrat (58) par application sur une petite zone (72) du substrat d'une couche (73) d'une matière hydrophile sur laquelle la cellule vient se fixer.
EP00937916A 1999-05-28 2000-05-30 Appareils et procedes d'evaluation du transport cellulaire pour la reserche, la decouverte et les tests d'un medicament, les diagnostics cliniques et l'elaboration d'une therapie Withdrawn EP1190246A2 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13713499P 1999-05-28 1999-05-28
US137134P 1999-05-28
PCT/US2000/014805 WO2000073784A2 (fr) 1999-05-28 2000-05-30 Appareils et procedes d'evaluation du transport cellulaire pour la reserche, la decouverte et les tests d'un medicament, les diagnostics cliniques et l'elaboration d'une therapie

Publications (1)

Publication Number Publication Date
EP1190246A2 true EP1190246A2 (fr) 2002-03-27

Family

ID=22475976

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00937916A Withdrawn EP1190246A2 (fr) 1999-05-28 2000-05-30 Appareils et procedes d'evaluation du transport cellulaire pour la reserche, la decouverte et les tests d'un medicament, les diagnostics cliniques et l'elaboration d'une therapie

Country Status (3)

Country Link
EP (1) EP1190246A2 (fr)
AU (1) AU5302900A (fr)
WO (1) WO2000073784A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10202887B4 (de) * 2002-01-25 2004-05-06 Advalytix Ag Zellanalyseverfahren
US10591462B2 (en) 2016-03-03 2020-03-17 Mohammad Abdolahad Electrochemical method and device for detecting the effect of anticancer drugs
WO2021134133A1 (fr) * 2020-01-03 2021-07-08 The University Of Manitoba Procédés et appareil de détection électrochimique pour déterminer l'absorption et la rétention de médicament dans des cellules

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4128456A (en) * 1977-10-11 1978-12-05 Lee Kai S Suction electrode for intracellular potential measurement
JPH0629734Y2 (ja) * 1988-04-20 1994-08-10 株式会社成茂科学器械研究所 硝子電極等の微動操作器
WO1990004645A1 (fr) * 1988-10-21 1990-05-03 Molecular Devices Corporation Procede et appareil permettant de detecter l'effet qu'ont sur des cellules vivantes des agents agissant sur les cellules
US5278048A (en) * 1988-10-21 1994-01-11 Molecular Devices Corporation Methods for detecting the effect of cell affecting agents on living cells
FR2733055B1 (fr) * 1995-04-12 1997-12-19 Chemodyne Sa Nouveau dispositif d'etude de cultures organotypiques et ses applications en electrophysiologie
IL119066A0 (en) * 1996-08-14 1996-11-14 Technion R & D Foundation Ltd Transmembrane movement and multidrug resistance
SE9702112D0 (sv) * 1997-06-04 1997-06-04 Holdingbolaget Vid Goeteborgs Method and apparatus for detection of a receptor antagonist
DE19744649C2 (de) * 1997-10-09 2003-03-27 Fraunhofer Ges Forschung Verfahren zur Messung bioelektrischer Signale von Zellen nach der Patch-Clamp-Methode sowie Verwendung einer Vorrichtung hierzu

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0073784A3 *

Also Published As

Publication number Publication date
AU5302900A (en) 2000-12-18
WO2000073784A3 (fr) 2001-07-19
WO2000073784A2 (fr) 2000-12-07

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