EP1161996A2 - Support de cuvettes - Google Patents

Support de cuvettes Download PDF

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Publication number
EP1161996A2
EP1161996A2 EP01113342A EP01113342A EP1161996A2 EP 1161996 A2 EP1161996 A2 EP 1161996A2 EP 01113342 A EP01113342 A EP 01113342A EP 01113342 A EP01113342 A EP 01113342A EP 1161996 A2 EP1161996 A2 EP 1161996A2
Authority
EP
European Patent Office
Prior art keywords
cuvette
stand
insertion portion
cuvettes
storing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01113342A
Other languages
German (de)
English (en)
Other versions
EP1161996A3 (fr
Inventor
Taisuke Hirono
Hiroki Matsui
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kowa Co Ltd
Original Assignee
Kowa Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kowa Co Ltd filed Critical Kowa Co Ltd
Publication of EP1161996A2 publication Critical patent/EP1161996A2/fr
Publication of EP1161996A3 publication Critical patent/EP1161996A3/fr
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/06—Test-tube stands; Test-tube holders

Definitions

  • This invention relates to a cuvette stand to be used at the time of counting number of leukocyte mixed in blood products.
  • FIG. 1 is a view for showing a cuvette (a container for measurement).
  • a cuvette 1 which is the container for inserting blood products and reagent therein, has a hollow main body 2 made of colorless, and transparent plastic and a colored lid 3 made of rubber, as shown in Fig. 1.
  • the main body 2 has a taper portion 2b and a plate portion 2a on the lower end thereof, and has almost circular cone shape facing the lower hand as a whole.
  • the main body 2 is open facing the upper hand, and the lid 3 is attachably and detachably installed on its opening portion.
  • Fig.2 is a view for showing a conventional cuvette stand 5.
  • the cuvette stand 5 has a main body comprised so as to unite, made of plastic. As shown in Fig.2, the main body has a horizontal plate portion 5c and leg portions 5b respectively provided on both ends of the plate portion 5c, formed in the perpendicular direction, and is formed so as to hold its upper plate portion 5c by these leg portions 5b, 5b.
  • eight (8) insertion portions 5a are formed, being arranged in a line at equal intervals.
  • the insertion portion 5a is 9.7mm in its diameter, for instance, and has such a size that the cuvette 1 can abut on an abutting portion 2d on the taper surface of the outer periphery of the taper portion 2b when the cuvette 1 is inserted and can be located.
  • twelve (12) rows of the stands 5 are used.
  • the cuvette 1 in which the reagent and the blood products are reacted is taken out of the cuvette stand 5 so as to centrifuge. And, the cuvette 1 is taken out of the centrifugal so as to set on a micro-leukocytometer.
  • laser beams are exposed to the leukocyte staying on the lower hand of the cuvette 1, and the image is analyzed with a CCD camera or the like from the lower hand so as to count the number of the leukocyte.
  • the conventional cuvette stand 5 has the insertion portion 5a on the plate portion 5c of the upper portion.
  • the abutting portion 2d on the taper portion 2b of the cuvette 1 abuts on the cuvette stand and the cuvette is located.
  • the insertion portion 5a should be formed so as to pass through the plate portion 2a of the top end of the cuvette 1 and so as not to exceed the maximum diameter of the taper portion 2b.
  • the insertion portion 5a is designed as a circle of 9.7mm, for instance, and the plate portion 2a is designed as a square of 6mm (the length of a diagonal 2e of Fig.1(b) is about 8.5mm) in its side, for instance.
  • Fig.4(a) is a view for showing the state of taking the cuvette 1 out of the conventional cuvette stand 5. If the cuvette 1 is shifted 0.6mm or more from the center at the time of taking as shown in Fig.4(a), the plate portion 2a of the top end of the cuvette 1 catches the insertion portion 5a, then, it is difficult to take out.
  • the object of the present invention is to provide cuvette stand wherein the cuvette can be inserted and taken out without catching somewhere and stand with cuvettes wherein cuvette finished operation and cuvette not yet finished operation can be easily differentiated from each other at the time of operation.
  • the invention of claim 1 is cuvette stand capable of inserting and locating a cuvette, comprising:
  • the cuvette is guided by the side wall at the time of inserting and taking the cuvette and can be taken out without catching somewhere.
  • the invention of claim 2 is the cuvette stand as set forth in claim 1, wherein said main body is made of transparent member.
  • the cuvette stand is made of transparent member. Then, the whole cuvette inserted in and located on the cuvette stand can be seen and confirmed so as to easily confirm the inside.
  • the invention of claim 3 is the cuvette stand as set forth in claim 1, wherein a visible portion is formed on side portion of said main body, corresponding to said each cuvette storing space.
  • the side portion of the cuvette stand has the visible portion. Then, the inside of the cuvette can be easily confirmed in the state of inserting and locating the cuvette in and on the cuvette stand.
  • the invention of claim 4 is the cuvette stand as set forth in claim 3, wherein said visible portion is made of transparent member.
  • the visible portion is made of transparent material. Then, the inside of the cuvette can be easily confirmed in the state of inserting and locating the cuvette in and on the cuvette stand.
  • the invention of claim 5 is the cuvette stand as set forth in claim 3, wherein said visible portion is comprised of a slit.
  • the visible portion is comprised of the slit. Then, the inside of the cuvette can be easily confirmed in the state of inserting and locating the cuvette in and on the cuvette stand.
  • the invention of claim 6 is stand with cuvettes in the cuvette stand as set forth in any of claims 1 trough 5, wherein in a plurality of said cuvette storing portions on said main body of said cuvette stand, said cuvettes which number is fewer than the number of said cuvette storing portions are stored.
  • the stand with cuvettes stores the cuvettes, which number is fewer than one of the cuvette storing portion, in a plurality of the cuvette storing portions on the main body. Even if an operation is interrupted or during an operation, then, the cuvette not yet finished operation and the cuvette finished operation can be easily differentiated from each other. Besides, by using the stand with cuvettes of the present invention, high accurate measurement operation can be immediately executed without preparing extra space for the cuvettes.
  • the side wall is provided so as to continuously connect with the insertion portion and so as to form the cuvette storing space. Then, when the cuvette is taken out of the cuvette storing portion, the side wall can guide the cuvette.
  • the whole cuvette located can be seen through.
  • the visible portion on the side portion of the cuvette stand corresponding to each cuvette storing space, the inside of the cuvette located can be seen.
  • the visible portion by transparent member By forming the visible portion by transparent member, the inside of the cuvette located can be seen through the transparent member.
  • the visible portion by a slit By forming the visible portion by a slit, the inside of the cuvette located can be seen through the slit.
  • the cuvettes which number is fewer than one of the cuvette storing portion are stored in a plurality of the cuvette storing portions on the main body of the cuvette stand. Then, the stand always have the storing portion on which the cuvette is not located.
  • FIG.3 is a view for showing an embodiment of the cuvette stand according to the present invention.
  • a cuvette stand 6 is for inserting and locating the cuvette 1 (dedicated cuvette), and has a main body 8 which shape is thin rectangular parallelopiped as a whole, as shown in Fig.3.
  • the main body 8 is formed so as to unite by transparent plastic (polystyrene in this embodiment).
  • the main body 8 has side outer wall 6b which is outer side in four directions, and has a plate portion 6c which is the upper face of the main body, supported by the side outer wall 6b.
  • each cuvette storing portion 7 On the plate portion 6c, nine (9) cuvette storing portions 7 are formed, and these cuvette storing portions are arranged in a line at equal intervals in the center of the width direction (in up and down direction in Fig.3(a)).
  • An insertion portion 6a which diameter is D1 is provided with the cuvette storing portion 7 on the plate portion 6c.
  • a hollow cylindrical body 6d On the lower hand of each insertion portion 6a in the perpendicular direction, a hollow cylindrical body 6d, which diameter is D1, the same as the insertion portion 6a, is provided so as to continuously connect with the insertion portion 6a.
  • a cuvette storing space 9 in cylindrical shape is formed extending each insertion portion 6a and each cylindrical body 6d.
  • the storing space 9 is enclosed with the insertion portion 6a on the plate portion 6c, a side wall 6e formed by the cylindrical body 6d, and a bottom plate portion 6f.
  • the side wall 6e of the cylindrical body 6d and the insertion portion 6a of the plate portion 6c are communicated with each other, and are unitedly formed so as not to catch somewhere.
  • numerals "0" through “8” are affixed as the identification number n corresponding to each insertion portion 6a.
  • the cuvette 1 In order to insert and locate the cuvette 1 into and on the cuvette stand 6, the cuvette 1 is inserted into the storing portion 7, as shown in Fig.3(b). Then, the taper portion 2b of the lower portion of the cuvette 1 and the cuvette stand 6 abut on each other in the abutting portion 2d on the taper portion 2b and the insertion portion 6a of the cuvette stand 6. Then, the cuvette 1 is located in such a manner that the upper portion is above the plate portion 6c of the cuvette stand 6 and the lower portion is entered into the storing space 9 of the lower hand of the plate portion 6c, with the abutting portion 2d as its boundary.
  • the present cuvette stand 6 is comprised as mentioned before.
  • the operation of counting number of leukocyte mixed in blood products with the cuvette stand 6 will now be explained hereinafter, referring to the drawings.
  • Fig. 5 is a view for showing a procedure of taking and locating the cuvette in the cuvette stand 6.
  • measurement is executed for twelve (12) sets with eight (8) cuvettes 1 as a set.
  • the present cuvette stand 6 has nine (9) insertion portions 6a one more than eight (8) insertion portions 5a of the conventional cuvette stand 5 (see Fig.2).
  • 12 (twelve) rows X 8 (eight) ninety six (96) of the cuvettes 1 are inserted in and located on "1" through “8” as the identification numbers n of the insertion portions 6a in the storing portions 7 of each cuvette stand 6.
  • the insertion portion 6a of "0" as the identification number n which is on the utmost left hand of the figure, is a spare insertion portion 6a-0 since no cuvette 1 is located therein.
  • Fig.4(b) is a view for showing the state of taking the cuvette 1 out of the cuvette stand 6 of the present embodiment.
  • the cuvette stand 6 has the side wall 6e in cylindrical shape on the insertion portion 6a. Then, the plate portion 2a of the top end of the cuvette contacts with the side wall 6e of the cuvette stand 6 even if the cuvette 1 is shifted from the center of the insertion portion 6a when the cuvette 1 is taken out, as shown in Fig.4(b).
  • the side wall 6e is continuously formed with the same diameter D 1 in its whole length and at the insertion portion 6a.
  • the plate portion 2a of the top end of the cuvette is slipped for the upper hand without catching somewhere, different from the conventional way so as to guide to the exist of the insertion portion 6a.
  • the operation of taking ninety six (96) cuvettes 1 out of the cuvette stand 6 can be easily executed several times without catching.
  • the reagent is added to all of the ninety six (96) cuvettes 1
  • blood 100 ⁇ L is extracted from the blood products and the blood extracted is added to the cuvette 1 to which the reagent is added, taken out of the cuvette stand 6 so as to mix and react, and thereafter it is inserted in and located on the cuvette stand 6, again.
  • the blood products is injected into the cuvette 1 taken out of the insertion portion 6a-1, so as to mix and react, and then the cuvette 1 is inserted into and located on the spare insertion portion 6a-0. Then, the cuvette insertion portion 6a-1 in which the cuvette 1 has been inserted becomes empty. Subsequently, the blood products is added to the cuvette 1 taken out of the insertion portion 6a-2, and they are mixed so as to react, and the cuvette 1 is inserted in and located on the insertion portion 6a-1 which newly becomes empty. Then, the insertion portion 6a-2 becomes empty by moving the cuvette 1 to the insertion portion 6a-1, similar to the above-mentioned.
  • the blood is added to the cuvette 1 taken out of the insertion portion 6a-n, and they are mixed so as to react, then as shown in Fig.5(b), the cuvette 1 is inserted in and located on the insertion portion 6a-(n-1) which newly becomes empty by the operation just before, adjacent to the insertion portion 6a-n in which the cuvette 1 has been inserted, so as to make the insertion portion 6a-n in which the cuvette 1 has been inserted empty.
  • This operation is repeated for eight (8) cuvettes 1, and the operation is executed for eight (8) cuvettes 1 (see Fig.5(c)).
  • the reagent is changed. So, a tester can easily confirm to which cuvette 1 the blood products is added and mixed by seeing through the inside of the cuvette 1 from the side of the cuvette stand 6. Therefore, the operation of inserting and locating the cuvette 1 in and on the cuvette stand 6 using the empty insertion portion has no problem.
  • the blood products have light yellow color as blood platelet products or blood plasma products, but, the color is almost never changed when reagent is added so as to mix, then it is difficult to differentiate the reagent to which the products have not been yet added from the other.
  • the insertion portions 6a (nine insertion portions) which number is one more than the number of a set of the cuvettes 1 to be used for measurement are formed, as shown in Fig.5. Then, the cuvettes 1 which number is fewer than the number of the insertion portions 6a (eight cuvettes) are located, and the cuvettes 1 are taken out and located on so as to position one empty insertion portion 6a (the cuvette 1 is not located therein) between the cuvette 1 finished operation and the cuvette 1 not yet finished operation.
  • the number of the insertion portions 6a is made one more than the number of the cuvettes 1 to be inserted into the cuvette stand 6 in the present embodiment, but a plurality of spare insertion portions may be prepared.
  • the above-mentioned operation of injecting and mixing blood is respectively executed with twelve (12) rows of the cuvette stands 6. Thereafter, the cuvette 1, in which reagent and blood products are reacted, is taken out of the cuvette stand 6 so as to centrifuge. Then, the cuvette 1 is taken out of a centrifugal so as to set on a micro-leukocytometer. In this machine, laser beams are exposed to the leukocyte staying on the lower hand of the cuvette 1, and the image is analyzed with a CCD camera or the like from the lower hand so as to count the number of the leukocyte.
  • Fig.6 is a view for showing another embodiment of the cuvette stand 6.
  • the inside of the cuvette can be easily confirm by making the whole by transparent member.
  • another method is possible. For instance, even if transparent material can not be used for the whole cuvette stand 6, the portion from a part of the side wall 6e corresponding to each cuvette storing space 9 to the side outer wall 6b of the cuvette stand 6 in the side portion of the cuvette stand 6 may be formed by transparent material 11 so as to make this portion a visible portion 10 for seeing and confirming the contents of each cuvette 1, as shown in Fig.6(a).
  • a slit 13 may be formed on the portion from a part of the side wall 6e corresponding to each cuvette storing space 9 to the side outer wall 6b of the cuvette stand 6 so as to make this portion the visible portion 10 for seeing and confirming the contents of each cuvette 1.
  • Fig.7 is a view for showing another embodiment of the cuvette stand 6 (the numerals and marks in Fig.7 are the portions corresponding to the cuvette stand 6 as shown in Fig.3 already explained.).
  • the side wall 6e formed in cylindrical shape is used.
  • a cuvette stand 14 may have the insertion portion 6a in the shape of a quadrangle, or the cuvette stand 14 may have the insertion potion in the shape of a triangle or a polygon rather than a triangle as long as it has the side wall 6e forming the storing space 9 continuously connecting with the insertion portion 6a and the side wall can guide the cuvette to the exit by slipping the plate portion 2a thereon without catching the plate portion 2a of the top end of the cuvette.
  • the insertion portion may be ellipse shape seen from a plane. That is, the plane shape of the insertion portion 6a may not be always circular shape.
  • a cuvette stand 15 may have the continuous side wall which sectional area of the storing space 9 expands for the lower hand although the side wall 6e is formed along the direction of inserting and taking, that is, C-D direction in the above-mentioned embodiment.
  • a cuvette stand 16 may have a taper portion 6g having the same inclination as the taper portion 2b of the cuvette on a part of the side wall under the insertion portion 6a in order to settle the cuvette 1 at the time of inserting in and locating on the stand. That is, any cuvette stand is available as long as it has a continuous side wall and the cuvette can be inserted therein and taken out thereof without catching somewhere.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
EP01113342A 2000-06-06 2001-06-01 Support de cuvettes Withdrawn EP1161996A3 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000168584A JP2001349825A (ja) 2000-06-06 2000-06-06 キュベット・スタンド及びキュベット付きスタンド
JP2000168584 2000-06-06

Publications (2)

Publication Number Publication Date
EP1161996A2 true EP1161996A2 (fr) 2001-12-12
EP1161996A3 EP1161996A3 (fr) 2003-08-06

Family

ID=18671570

Family Applications (2)

Application Number Title Priority Date Filing Date
EP01113342A Withdrawn EP1161996A3 (fr) 2000-06-06 2001-06-01 Support de cuvettes
EP01113704A Pending EP1161997A2 (fr) 2000-06-06 2001-06-02 Support pour cuvettes

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP01113704A Pending EP1161997A2 (fr) 2000-06-06 2001-06-02 Support pour cuvettes

Country Status (3)

Country Link
US (1) US20020009397A1 (fr)
EP (2) EP1161996A3 (fr)
JP (1) JP2001349825A (fr)

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Publication number Priority date Publication date Assignee Title
JP3791384B2 (ja) * 2001-10-11 2006-06-28 株式会社島津製作所 光分析用多連装セル
US7259007B2 (en) * 2003-12-24 2007-08-21 Glycofi, Inc. Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins
SE528697C2 (sv) * 2005-03-11 2007-01-30 Hemocue Ab Volymetrisk bestämning av antalet vita blodkroppar i ett blodprov
EP1909094B1 (fr) * 2005-07-27 2017-05-03 Sysmex Corporation Cuvette
US7842247B2 (en) 2005-08-19 2010-11-30 Canadian Blood Services Sample holder for dynamic light scattering
US20080283481A1 (en) * 2007-05-18 2008-11-20 Evans Richard W Modular spice rack
DE102007030384B4 (de) * 2007-06-29 2009-02-05 Dade Behring Marburg Gmbh Verfahren zur Identifizierung eines transparenten Objekts anhand seines Absorptionsspektrums
US20210059450A1 (en) * 2019-08-28 2021-03-04 Apres Ski Company, LLC Drinking Shot Delivery And Novelty Apparatus
US11559139B2 (en) * 2020-09-30 2023-01-24 Todd ERVIN Wine rack apparatus

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US3175695A (en) * 1961-06-15 1965-03-30 Advance Scient Corp Tissue culture tube rack means
US4801428A (en) * 1986-10-27 1989-01-31 Becton, Dickinson And Company Blood sample sedimentation test kit
EP0953379A1 (fr) * 1998-05-01 1999-11-03 F. Hoffmann-La Roche Ag Dispositif pour surveiller simultanément des réactions se déroulant dans une pluralité de récipients de réaction

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US3649464A (en) * 1969-12-05 1972-03-14 Microbiological Ass Inc Assay and culture tray
US4411868A (en) * 1981-12-11 1983-10-25 Becton, Dickinson And Company Multiple tube rack
US4735778A (en) * 1985-08-28 1988-04-05 Kureha Kagaku Kohyo Kabushiki Kaisha Microtiter plate
FI87278C (fi) * 1989-08-28 1992-12-10 Labsystems Oy Kyvettmatris och staellning foer denna
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US5378433A (en) * 1993-11-15 1995-01-03 Akzo N.V. Sample tube rack and adapter
US5514343A (en) * 1994-06-22 1996-05-07 Nunc, As Microtitration system
DE4429155A1 (de) * 1994-08-17 1996-02-22 Hans Schiesl Meßanordnung und Verfahren zur Durchführung luminometrischer Reihenanalysen sowie Mehrfachküvette zur Aufnahme von Flüssigkeitsproben hierfür
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FR2764704B1 (fr) * 1997-06-16 1999-08-20 Stago Diagnostica Dispositif pour la lecture automatique d'un code d'identification porte par des recipients tubulaires

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3175695A (en) * 1961-06-15 1965-03-30 Advance Scient Corp Tissue culture tube rack means
US4801428A (en) * 1986-10-27 1989-01-31 Becton, Dickinson And Company Blood sample sedimentation test kit
EP0953379A1 (fr) * 1998-05-01 1999-11-03 F. Hoffmann-La Roche Ag Dispositif pour surveiller simultanément des réactions se déroulant dans une pluralité de récipients de réaction

Also Published As

Publication number Publication date
US20020009397A1 (en) 2002-01-24
EP1161997A2 (fr) 2001-12-12
EP1161996A3 (fr) 2003-08-06
JP2001349825A (ja) 2001-12-21

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