EP1161142A1 - Kulturverfahren für flüssigkeitsgefüllte sensorische organe - Google Patents

Kulturverfahren für flüssigkeitsgefüllte sensorische organe

Info

Publication number
EP1161142A1
EP1161142A1 EP00914825A EP00914825A EP1161142A1 EP 1161142 A1 EP1161142 A1 EP 1161142A1 EP 00914825 A EP00914825 A EP 00914825A EP 00914825 A EP00914825 A EP 00914825A EP 1161142 A1 EP1161142 A1 EP 1161142A1
Authority
EP
European Patent Office
Prior art keywords
fluid
culture
filled
sensory organ
filled sensory
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00914825A
Other languages
English (en)
French (fr)
Other versions
EP1161142A4 (de
Inventor
Jonathan Kil
Hubert Lowenheim
Anish H. Sudra
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Otogene AG
Otogene USA Inc
Original Assignee
Jonathan Kil
Hubert Lowenheim
Anish H. Sudra
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jonathan Kil, Hubert Lowenheim, Anish H. Sudra filed Critical Jonathan Kil
Publication of EP1161142A1 publication Critical patent/EP1161142A1/de
Publication of EP1161142A4 publication Critical patent/EP1161142A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/062—Sensory transducers, e.g. photoreceptors; Sensory neurons, e.g. for hearing, taste, smell, pH, touch, temperature, pain
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • A61K38/1709—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors

Definitions

  • the present invention provides methods tor culturing fluid-filled sensory organs /// vit/ o
  • the present invention provides methods for culturing the eyeball, including the retina, and the bony labyrinth, including the Organ of Corti, in vit/o
  • the methods of the present invention involve little or no microsurgical dissection of the entire fluid-filled sensory organ, thereby preserving the structural and functional integrity of the sensory epithelium
  • Example 1 Excision and In Vitro Culture of Mouse Inner Ear
  • the inner ear of a mouse was excised in the following manner. Postnatal day 7-14 Swiss Webster mice were decapitated and their skulls immersed in 70% ethanol for 5 min to disinfect. Under sterile conditions, the skull was cut into halves along the mid-sagittal axis and placed into 3 ml of culture media (NeuralbasalTM Media at pH 7.4; Gibco) in a 35 mm plastic culture dish (Nalge Nunc International, 2000 North Aurora Road, Naperville, 1L 60563) Using surgical forceps, the bony inner ear labyrinth was visualized and separated from the temporal bone The overlying connective tissue, stapes bone, facial nerve and stapedial artery were removed Using a fine forcep, a small hole about 2 mm in diameter was made through the apical turn of the lateral cochlear wall This surgically created conduit, along with the patent oval and round windows of the cochlea, permit ready diffusion of

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Neurosurgery (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Acoustics & Sound (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Prostheses (AREA)
EP00914825A 1999-03-05 2000-03-03 Kulturverfahren für flüssigkeitsgefüllte sensorische organe Withdrawn EP1161142A4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12310099P 1999-03-05 1999-03-05
US123100P 1999-03-05
PCT/US2000/005736 WO2000054583A1 (en) 1999-03-05 2000-03-03 Methods for culturing fluid-filled sensory organs

Publications (2)

Publication Number Publication Date
EP1161142A1 true EP1161142A1 (de) 2001-12-12
EP1161142A4 EP1161142A4 (de) 2003-01-22

Family

ID=22406715

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00914825A Withdrawn EP1161142A4 (de) 1999-03-05 2000-03-03 Kulturverfahren für flüssigkeitsgefüllte sensorische organe

Country Status (4)

Country Link
EP (1) EP1161142A4 (de)
AU (1) AU3616400A (de)
CA (1) CA2364024A1 (de)
WO (1) WO2000054583A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7390653B2 (en) * 2002-12-04 2008-06-24 Synthecon, Inc. Culture chamber for biologicals
US8338114B1 (en) 2007-04-19 2012-12-25 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Engineered human broncho-epithelial tissue-like assemblies

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995007344A1 (en) * 1993-09-09 1995-03-16 Schwarz Ray P Gas permeable bioreactor and method of use

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5308764A (en) * 1988-06-30 1994-05-03 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Multi-cellular, three-dimensional living mammalian tissue

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995007344A1 (en) * 1993-09-09 1995-03-16 Schwarz Ray P Gas permeable bioreactor and method of use

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CH.-P. LING & M. BOEHNKE: "iNFLUENCES OF METHYLCELLULOSE ON CORNEAL EPITHELIAL WOUND HEALING." J. OCULAR PHARMACOL. THERAPEUT., vol. 15, no. 1, 1999, pages 59-63, XP001119933 *
M. ANNIKO: "Antibiotics and sensorineural interactions." ACTA OTOLARYNGOL. (STOCKH), vol. S429, 1986, pages 17-21, XP002222356 *
See also references of WO0054583A1 *

Also Published As

Publication number Publication date
EP1161142A4 (de) 2003-01-22
AU3616400A (en) 2000-10-04
CA2364024A1 (en) 2000-09-21
WO2000054583A1 (en) 2000-09-21

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Inventor name: SUDRA, ANISH H.

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