EP1148139A4 - Luziferase-Variante, Gen der Luziferase-Variante, rekombinante DNA und Verfahrenzur Herstellung der Luziferase-Variante - Google Patents

Luziferase-Variante, Gen der Luziferase-Variante, rekombinante DNA und Verfahrenzur Herstellung der Luziferase-Variante

Info

Publication number
EP1148139A4
EP1148139A4 EP99959952A EP99959952A EP1148139A4 EP 1148139 A4 EP1148139 A4 EP 1148139A4 EP 99959952 A EP99959952 A EP 99959952A EP 99959952 A EP99959952 A EP 99959952A EP 1148139 A4 EP1148139 A4 EP 1148139A4
Authority
EP
European Patent Office
Prior art keywords
variant luciferase
variant
luciferase
recombinant dna
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP99959952A
Other languages
English (en)
French (fr)
Other versions
EP1148139A1 (de
EP1148139B1 (de
Inventor
Naoki Kajiyama
Eiichi Nakano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kikkoman Corp
Original Assignee
Kikkoman Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kikkoman Corp filed Critical Kikkoman Corp
Publication of EP1148139A1 publication Critical patent/EP1148139A1/de
Publication of EP1148139A4 publication Critical patent/EP1148139A4/de
Application granted granted Critical
Publication of EP1148139B1 publication Critical patent/EP1148139B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP99959952A 1999-01-05 1999-12-21 Luziferase-variante, gen der luziferase-variante, rekombinante dna und verfahrenzur herstellung der luziferase-variante Expired - Lifetime EP1148139B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP26999A JP3694181B2 (ja) 1999-01-05 1999-01-05 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び変異型ルシフェラーゼの製造法
JP26999 1999-01-05
PCT/JP1999/007192 WO2000040732A1 (fr) 1999-01-05 1999-12-21 Variant de luciferase, gene de variant de luciferase, adn recombinant et technique permettant de produire un variant de luciferase

Publications (3)

Publication Number Publication Date
EP1148139A1 EP1148139A1 (de) 2001-10-24
EP1148139A4 true EP1148139A4 (de) 2002-07-10
EP1148139B1 EP1148139B1 (de) 2004-08-25

Family

ID=11469198

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99959952A Expired - Lifetime EP1148139B1 (de) 1999-01-05 1999-12-21 Luziferase-variante, gen der luziferase-variante, rekombinante dna und verfahrenzur herstellung der luziferase-variante

Country Status (3)

Country Link
EP (1) EP1148139B1 (de)
JP (1) JP3694181B2 (de)
WO (1) WO2000040732A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5188771B2 (ja) 2007-09-26 2013-04-24 株式会社日立製作所 変異型ホタルルシフェラーゼ
JP2011120559A (ja) 2008-12-26 2011-06-23 Kikkoman Corp ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法
DK3181687T3 (en) * 2009-05-01 2018-09-10 Promega Corp Synthetic oplophorus luciferases with increased light yield
WO2012061477A1 (en) 2010-11-02 2012-05-10 Promega Corporation Coelenterazine derivatives and methods of using same
US8669103B2 (en) * 2010-11-02 2014-03-11 Promega Corporation Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
JP6493316B2 (ja) 2016-06-21 2019-04-03 東亜ディーケーケー株式会社 変異型甲虫ルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型甲虫ルシフェラーゼの製造方法
JPWO2021162123A1 (de) 2020-02-14 2021-08-19

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5219737A (en) * 1990-03-27 1993-06-15 Kikkoman Corporation Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase
DE69535744T2 (de) * 1994-01-03 2009-07-02 Promega Corp., Madison Mutierte luziferasen

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DEMENTIEVA E I ET AL: "PHYSICOCHEMICAL PROPERTIES OF RECOMBINANT LUCIOLA MINGRELICA LUCIFERASE AND ITS MUTANT FORMS", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 1, no. 61, 1996, pages 115 - 119, XP002078631, ISSN: 0006-2960 *
DEVINE JERRY H ET AL: "Luciferase from the East European firefly Luciola mingrelica: Cloning and nucleotide sequence of the cDNA, overexpression in Escherichia coli and purification of the enzyme.", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1173, no. 2, 28 May 1993 (1993-05-28), pages 121 - 132, XP001070250, ISSN: 0006-3002 *
See also references of WO0040732A1 *

Also Published As

Publication number Publication date
JP3694181B2 (ja) 2005-09-14
JP2000197484A (ja) 2000-07-18
EP1148139A1 (de) 2001-10-24
EP1148139B1 (de) 2004-08-25
WO2000040732A1 (fr) 2000-07-13

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