EP1144619A1 - Procede d'ultrafiltration - Google Patents

Procede d'ultrafiltration

Info

Publication number
EP1144619A1
EP1144619A1 EP00928724A EP00928724A EP1144619A1 EP 1144619 A1 EP1144619 A1 EP 1144619A1 EP 00928724 A EP00928724 A EP 00928724A EP 00928724 A EP00928724 A EP 00928724A EP 1144619 A1 EP1144619 A1 EP 1144619A1
Authority
EP
European Patent Office
Prior art keywords
pressure differential
membrane
constant pressure
ultrafiltration
volume
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00928724A
Other languages
German (de)
English (en)
Inventor
Jack T. Leonard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Millipore Corp
Original Assignee
Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Millipore Corp filed Critical Millipore Corp
Priority claimed from PCT/US2000/011926 external-priority patent/WO2000066723A1/fr
Publication of EP1144619A1 publication Critical patent/EP1144619A1/fr
Withdrawn legal-status Critical Current

Links

Definitions

  • US Patent 5,679,310 suggests using a vacuum to filter a multiple well plate which utilizes ultrafiltration or microporous hollow fibers or other large surface area configurations of membranes instead of flat sheet membranes
  • the hollow fibers are reported to be used in lieu of flat sheet membranes in order to increase the total surface area of the membrane over that available with a flat sheet
  • this product has never been commercialized
  • the same process can be used with a multiple welled plate, such as is disclosed in U S Patents 4,902,481 and 5,047,215
  • These plates are commercially available in a number of configurations, generally containing from about 6 to 1536 wells per plate
  • the most common plate has 96 wells and is available from Millipore Corporation as the MultiScreen® 96 well plate
  • the plate can be mounted on top of a vacuum manifold such that the vacuum is applied to the downstream side of the filter A sample to be filtered is placed into one or more of the wells and the vacuum is applied at a force and time sufficient to reach the desired level of filtration
  • a positive manifold can be mounted on the upstream side of the membrane and a positive pressure differential can be applied to the liquid at a force and time sufficient to achieve the desired filtration

Landscapes

  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

L'invention se rapporte à un procédé d'ultrafiltration mettant en oeuvre un différentiel de pression constant pour appliquer une force d'entraînement. Ce procédé est particulièrement destiné à la concentration ou à la purification de protéines et/ou d'acides nucléiques, processus pour lesquels il permet souvent d'éviter une ou plusieurs étapes de diafiltration. Ledit procédé convient particulièrement aux applications à petits volumes, du type dispositifs concentrateurs de petite taille et plaques à puits multiples, qui utilisent généralement des volumes de départ de liquides inférieurs à 500 microlitres. Le procédé consiste à placer un volume de liquide au-dessus d'une membrane d'ultrafiltration et à appliquer un différentiel de pression constant au moyen d'une force et pendant un laps de temps permettant d'obtenir la concentration souhaitée du côté amont de la membrane. Le concentré est ensuite dilué ou retiré en vue d'un traitement ultérieur.
EP00928724A 1999-05-04 2000-05-02 Procede d'ultrafiltration Withdrawn EP1144619A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US13236999P 1999-05-04 1999-05-04
US132369P 1999-05-04
US18235700P 2000-02-14 2000-02-14
US182357P 2000-02-14
PCT/US2000/011926 WO2000066723A1 (fr) 1999-05-04 2000-05-02 Procede d'ultrafiltration

Publications (1)

Publication Number Publication Date
EP1144619A1 true EP1144619A1 (fr) 2001-10-17

Family

ID=26830300

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00928724A Withdrawn EP1144619A1 (fr) 1999-05-04 2000-05-02 Procede d'ultrafiltration

Country Status (2)

Country Link
EP (1) EP1144619A1 (fr)
DE (1) DE1144619T1 (fr)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0066723A1 *

Also Published As

Publication number Publication date
DE1144619T1 (de) 2002-11-14

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Legal Events

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Effective date: 20061201