Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
  • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
  • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
  • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose

Definitions

• This invention relates to hygiene monitoring, and in particular to an assay for the presence of food residues and/or microorganisms.
• Hygiene monitoring procedures typically involve swabbing a surface, and analysing the sample taken up in the swab, for the presence of food residues and/or microorganisms.
• a typical hygiene monitoring device is disclosed in WO-A-98/27196.
• the ability to detect the presence of food residues and/or microorganisms in the sample that has been taken up by swabbing relies on the presence of ADP/ATP, and the ability to amplify the amount of ADP/ATP, and convert it to a detectable signal, also as disclosed in WO-A-94/25619.
• the preferred amplification reaction involves, inter alia, the amplification of ATP and then the conversion of glucose-6-phosphate to glucose, and the conversion of glucose, via a sequence of enzymatic reactions, to a coloured end point.
• glucose itself may be a sufficient indicator, for the purposes of an assay used in hygiene monitoring.
• such an assay comprises the collection of a sample from a locus, and the determination of the presence of glucose in the sample.
• the reagents disclosed in WO-A-94/25619 include those sufficient to convert glucose to a detectable signal; if desired, the reagents used in this invention may exclude those that convert ATP, but the signal generated in the presence of glucose appears relatively rapidly, and can thus be distinguished, in addition to the advantage of providing a rapid response. The amplification of ATP needed to generate the signal is relatively slow.
• the locus to be tested is also assayed for the substantial absence of materials that may interfere in the novel assay, e.g. by inhibiting or by giving false positives, such as peroxide or reducing agents that may be incorporated in materials used to sanitise the locus.
• the present invention can be practised utilising the same reagents as are disclosed in WO-A-94/25619, especially in so far as that relates to the conversion of glucose to a detectable signal. If desired, the reagents may exclude one or more of the components that are disclosed there for the conversion of ATP to glucose.
• a suitable device that can be used for the purposes of swabbing and detection is disclosed in WO-A-98/27196 and WO-A-99/31218.
• Example 1 illustrates the utility of the present invention. All used the same amplification system, with a colour end-point, as disclosed in Example 1 of WO-A-94/25619.
• Example 1 illustrates the utility of the present invention. All used the same amplification system, with a colour end-point, as disclosed in Example 1 of WO-A-94/25619.
• Example 1 illustrates the utility of the present invention. All used the same amplification system, with a colour end-point, as disclosed in Example 1 of WO-A-94/25619.
• Example 1 illustrates the utility of the present invention. All used the same amplification system, with a colour end-point, as disclosed in Example 1 of WO-A-94/25619.
• Swabs were taken from a variety of locations, and tested. In addition to determining the total viable count (TVC) and Enterobacteria (Enteros), contact plates were used alongside two devices.
• systemSURE (“sSURE”) is a portable hygiene monitoring system (available from Becton Dickinson) that uses bioluminescence as an endpoint.
• the "Pen Swab” is generally as disclosed in WO-A-99/31218. Results are shown in Table 1.
• the colour reagents were used to determine the level at which glucose was detectable.
• the reagents were activated in the presence of different concentrations of glucose.
• the absorbance values for a complete colour change were obtained within 120 seconds. Results are shown in Table 2.
• Swabs were taken from a variety of locations in a store, at different times of day and tested. Results are shown, for two different days, in Tables 3A and 3B. A large proportion of the data shows a rapid colour change (in seconds), characteristic of glucose detection. The corresponding RLU values do not show the presence of gross amounts of ATP, which is consistent with the detection of glucose. Table 3A

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• Chemical & Material Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Organic Chemistry (AREA)
• Health & Medical Sciences (AREA)
• Engineering & Computer Science (AREA)
• Zoology (AREA)
• Wood Science & Technology (AREA)
• Proteomics, Peptides & Aminoacids (AREA)
• Molecular Biology (AREA)
• General Engineering & Computer Science (AREA)
• Immunology (AREA)
• Microbiology (AREA)
• Biophysics (AREA)
• Analytical Chemistry (AREA)
• Physics & Mathematics (AREA)
• Genetics & Genomics (AREA)
• Biochemistry (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Biotechnology (AREA)
• General Health & Medical Sciences (AREA)
• Emergency Medicine (AREA)
• Toxicology (AREA)
• Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
• Investigating Or Analysing Biological Materials (AREA)

Applications Claiming Priority (3)

Publications (1)

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• 1998
  • 1998-12-15 GB GBGB9827584.5A patent/GB9827584D0/en not_active Ceased
• 1999
  • 1999-12-15 WO PCT/GB1999/004244 patent/WO2000036139A1/en not_active Application Discontinuation
  • 1999-12-15 JP JP2000588387A patent/JP2002532698A/ja active Pending
  • 1999-12-15 EP EP99961211A patent/EP1141380A1/de not_active Withdrawn
  • 1999-12-15 AU AU17897/00A patent/AU1789700A/en not_active Abandoned

Non-Patent Citations (1)

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