EP1076239A3 - Revêtement en polymères hydrophiles réticulés - Google Patents
Revêtement en polymères hydrophiles réticulés Download PDFInfo
- Publication number
- EP1076239A3 EP1076239A3 EP00116757A EP00116757A EP1076239A3 EP 1076239 A3 EP1076239 A3 EP 1076239A3 EP 00116757 A EP00116757 A EP 00116757A EP 00116757 A EP00116757 A EP 00116757A EP 1076239 A3 EP1076239 A3 EP 1076239A3
- Authority
- EP
- European Patent Office
- Prior art keywords
- coating
- cross
- hydrophilic polymers
- linked hydrophilic
- capillaries
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000011248 coating agent Substances 0.000 title abstract 2
- 238000000576 coating method Methods 0.000 title abstract 2
- 229920001477 hydrophilic polymer Polymers 0.000 title abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000010382 chemical cross-linking Methods 0.000 abstract 1
- 239000003431 cross linking reagent Substances 0.000 abstract 1
- 230000003100 immobilizing effect Effects 0.000 abstract 1
- 229920000642 polymer Polymers 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44752—Controlling the zeta potential, e.g. by wall coatings
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19938002A DE19938002A1 (de) | 1999-08-11 | 1999-08-11 | Beschichtung mit quervernetzten hydrophilen Polymeren |
DE19938002 | 1999-08-11 |
Publications (3)
Publication Number | Publication Date |
---|---|
EP1076239A2 EP1076239A2 (fr) | 2001-02-14 |
EP1076239A3 true EP1076239A3 (fr) | 2001-05-30 |
EP1076239B1 EP1076239B1 (fr) | 2003-09-24 |
Family
ID=7918021
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP00116757A Expired - Lifetime EP1076239B1 (fr) | 1999-08-11 | 2000-08-03 | Procédé pour le revêtement des capillaires avec des polymères hydrophiles réticulés |
Country Status (6)
Country | Link |
---|---|
US (1) | US6596238B1 (fr) |
EP (1) | EP1076239B1 (fr) |
JP (1) | JP2001124737A (fr) |
AT (1) | ATE250762T1 (fr) |
CA (1) | CA2315479A1 (fr) |
DE (2) | DE19938002A1 (fr) |
Families Citing this family (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE9904802D0 (sv) * | 1999-12-23 | 1999-12-23 | Amersham Pharm Biotech Ab | Microfluidic surfaces |
EP1548429A4 (fr) * | 2002-09-05 | 2009-06-17 | Katayanagi Inst | Procede de separation de substances |
US7329545B2 (en) | 2002-09-24 | 2008-02-12 | Duke University | Methods for sampling a liquid flow |
US6911132B2 (en) | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
DE102004005337A1 (de) * | 2004-02-04 | 2005-08-25 | Studiengesellschaft Kohle Mbh | Mikrofluidische Chips mit immanenten hydrophilen Oberflächen |
JP4521754B2 (ja) * | 2004-03-31 | 2010-08-11 | 富山県 | キャピラリーチューブ及びその製造方法 |
JP5897780B2 (ja) | 2005-01-28 | 2016-03-30 | デューク ユニバーシティ | プリント回路基板上の液滴操作装置及び方法 |
EP1919618A2 (fr) * | 2005-05-21 | 2008-05-14 | Core-Microsolutions, Inc. | Attenuation de l'adsorption biomoleculaire avec des adjuvants polymeres hydrophiles |
EP2278303A3 (fr) | 2005-06-10 | 2012-02-22 | Life Technologies Corporation | Procédé et dispositif pour l'analyse génétique multiple |
US8613889B2 (en) * | 2006-04-13 | 2013-12-24 | Advanced Liquid Logic, Inc. | Droplet-based washing |
US8637317B2 (en) * | 2006-04-18 | 2014-01-28 | Advanced Liquid Logic, Inc. | Method of washing beads |
US8492168B2 (en) * | 2006-04-18 | 2013-07-23 | Advanced Liquid Logic Inc. | Droplet-based affinity assays |
US20140193807A1 (en) | 2006-04-18 | 2014-07-10 | Advanced Liquid Logic, Inc. | Bead manipulation techniques |
US9476856B2 (en) | 2006-04-13 | 2016-10-25 | Advanced Liquid Logic, Inc. | Droplet-based affinity assays |
US8658111B2 (en) | 2006-04-18 | 2014-02-25 | Advanced Liquid Logic, Inc. | Droplet actuators, modified fluids and methods |
US8716015B2 (en) | 2006-04-18 | 2014-05-06 | Advanced Liquid Logic, Inc. | Manipulation of cells on a droplet actuator |
US8809068B2 (en) * | 2006-04-18 | 2014-08-19 | Advanced Liquid Logic, Inc. | Manipulation of beads in droplets and methods for manipulating droplets |
US10078078B2 (en) | 2006-04-18 | 2018-09-18 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
US7851184B2 (en) * | 2006-04-18 | 2010-12-14 | Advanced Liquid Logic, Inc. | Droplet-based nucleic acid amplification method and apparatus |
WO2007123908A2 (fr) * | 2006-04-18 | 2007-11-01 | Advanced Liquid Logic, Inc. | Opérations en puits multiples à base de gouttelettes |
US7901947B2 (en) | 2006-04-18 | 2011-03-08 | Advanced Liquid Logic, Inc. | Droplet-based particle sorting |
US8637324B2 (en) | 2006-04-18 | 2014-01-28 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
US7439014B2 (en) | 2006-04-18 | 2008-10-21 | Advanced Liquid Logic, Inc. | Droplet-based surface modification and washing |
US8980198B2 (en) | 2006-04-18 | 2015-03-17 | Advanced Liquid Logic, Inc. | Filler fluids for droplet operations |
KR101553679B1 (ko) * | 2007-01-12 | 2015-09-17 | 보오드 오브 리젠츠, 더 유니버시티 오브 텍사스 시스템 | 저-흐름 분리 기법들의 인터페이스 연결 |
US9046514B2 (en) | 2007-02-09 | 2015-06-02 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods employing magnetic beads |
US8268246B2 (en) | 2007-08-09 | 2012-09-18 | Advanced Liquid Logic Inc | PCB droplet actuator fabrication |
GB2463750A (en) * | 2008-07-15 | 2010-03-31 | L3 Technology Ltd | Assay device comprising surfaces of different surface energies |
US9513253B2 (en) | 2011-07-11 | 2016-12-06 | Advanced Liquid Logic, Inc. | Droplet actuators and techniques for droplet-based enzymatic assays |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4796700A (en) * | 1984-06-25 | 1989-01-10 | Cities Service Oil And Gas Corporation | Process for retarding fluid flow |
EP0587156A1 (fr) * | 1992-09-11 | 1994-03-16 | Studiengesellschaft Kohle mbH | Désactivation des surfaces intérieures de capillaires |
US5322608A (en) * | 1992-12-23 | 1994-06-21 | Northeastern University | Siloxandediol coating for capillary electrophoresis and for general surface modification |
WO1996023221A1 (fr) * | 1995-01-27 | 1996-08-01 | Beckman Instruments, Inc. | Colonnes capillaires d'electrophorese revetues d'alcool de polyvinyle |
WO1999020378A1 (fr) * | 1997-10-20 | 1999-04-29 | Korea Research Institute Of Chemical Technology | Procede de fabrication de membrane composite a couche de revetement hydrophile sur membrane support hydrophobe |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4680201A (en) * | 1985-10-30 | 1987-07-14 | Stellan Hjerten | Coating for electrophoresis tube |
US4865707A (en) * | 1986-10-21 | 1989-09-12 | Northeastern University | Capillary gel electrophoresis columns |
US5221447A (en) * | 1991-12-06 | 1993-06-22 | Bio-Rad Laboratories, Inc. | Hydrophilic polymer coating of high pH stability for silica surfaces for suppression of electroendomosis and solute adsorption |
DE69633962T2 (de) * | 1995-01-27 | 2005-12-01 | Northeastern University, Boston | Verfahren zum Bilden einer kovalent gebundenen hydrophilen Schicht auf Basis von Polyvinyl-Alkohol für die Kapillarelektrophorese |
US5792331A (en) * | 1996-12-19 | 1998-08-11 | Dionex Corporation | Preformed polymer coating process and product |
-
1999
- 1999-08-11 DE DE19938002A patent/DE19938002A1/de not_active Withdrawn
-
2000
- 2000-08-03 DE DE50003808T patent/DE50003808D1/de not_active Expired - Lifetime
- 2000-08-03 AT AT00116757T patent/ATE250762T1/de not_active IP Right Cessation
- 2000-08-03 EP EP00116757A patent/EP1076239B1/fr not_active Expired - Lifetime
- 2000-08-09 US US09/634,770 patent/US6596238B1/en not_active Expired - Lifetime
- 2000-08-10 CA CA002315479A patent/CA2315479A1/fr not_active Abandoned
- 2000-08-11 JP JP2000244379A patent/JP2001124737A/ja active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4796700A (en) * | 1984-06-25 | 1989-01-10 | Cities Service Oil And Gas Corporation | Process for retarding fluid flow |
EP0587156A1 (fr) * | 1992-09-11 | 1994-03-16 | Studiengesellschaft Kohle mbH | Désactivation des surfaces intérieures de capillaires |
US5322608A (en) * | 1992-12-23 | 1994-06-21 | Northeastern University | Siloxandediol coating for capillary electrophoresis and for general surface modification |
WO1996023221A1 (fr) * | 1995-01-27 | 1996-08-01 | Beckman Instruments, Inc. | Colonnes capillaires d'electrophorese revetues d'alcool de polyvinyle |
WO1999020378A1 (fr) * | 1997-10-20 | 1999-04-29 | Korea Research Institute Of Chemical Technology | Procede de fabrication de membrane composite a couche de revetement hydrophile sur membrane support hydrophobe |
Also Published As
Publication number | Publication date |
---|---|
DE19938002A1 (de) | 2001-02-15 |
EP1076239B1 (fr) | 2003-09-24 |
DE50003808D1 (de) | 2003-10-30 |
EP1076239A2 (fr) | 2001-02-14 |
ATE250762T1 (de) | 2003-10-15 |
CA2315479A1 (fr) | 2001-02-11 |
US6596238B1 (en) | 2003-07-22 |
JP2001124737A (ja) | 2001-05-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1076239A3 (fr) | Revêtement en polymères hydrophiles réticulés | |
DK0991701T3 (da) | Hydrofilt overtræk og fremgangsmåde til fremstilling deraf | |
NO980981D0 (no) | Fremgangsmåte til fremstilling av antimikrobielle plaststoffer | |
AU2166001A (en) | Microfluidic surfaces | |
ZA975066B (en) | Durable hydrophilic coating for a porous hydrophobic polymer substrate. | |
EP1092758A3 (fr) | Composition durcissable, composition de revêtement, peinture, peinture antisalissure, produits durcis et procédé pour donner des propriétés antisalissures à une surface | |
WO2002060505A3 (fr) | Procede de revetement pour surfaces de dispositifs medicaux | |
PT952170E (pt) | Sistema de revestimento aquoso a partir de isocianatos de (met) acrilato de uretano com grupos isocianato endurecidos com uv | |
AU7638600A (en) | Topologically structured polymer coating | |
BR0014904A (pt) | Método de aperfeiçoar uma ligação entre uma superfìcie polimérica e um revestimento ou adesivo | |
AU1231899A (en) | Method for isolating and purifying nucleic acids on surfaces | |
BR0010083A (pt) | Processo de preparação de poliolefinas, poliolefinas comportando grupos ácidos parcialmente neutralizados, utilização de poliolefinas, e, processo de extrusão em espuma | |
ES2133888T3 (es) | Solido catalitico para la (co)polimerizacion de etileno, procedimiento para su preparacion, sistema catalitico para la (co)polimerizacion de etileno y procedimiento de (co)polimerizacion de etileno. | |
BR0014703A (pt) | Dispersão polimérica aquosa, processo para preparação da mesma, substrato ligado, e, uso de uma dispersão polimérica aquosa | |
EP1375537A3 (fr) | Polymères hydroabsorbants avec des molecules creuses supramoléculaires, leur procédé de préparation et leur utilisation | |
DK0861888T3 (da) | Anvendelse af tværbundne krystaller som en ny form for immobilisering af enzymer | |
WO2000063236A3 (fr) | Peptides modulant l'adhésion et méthodes d'utilisation | |
BR9605017A (pt) | Composiçao de revestimento curável tendo um componente contendo carbonato | |
ES2163180T3 (es) | Estructura absorbente que contiene un polimero altamente absorbente y articulo absorbente que contiene esta estructura absorbente. | |
EP1130667A4 (fr) | Malaxeur a coulisseau de presse, procede d'enrobage a l'aide d'un polymere conducteur ionique, et materiau en poudre | |
BR0207828B1 (pt) | composiÇço de revestimento de nço-isocianato reativa, sistema de revestimento e substrato revestido. | |
DE59607885D1 (de) | Adhäsives mittel auf wässriger basis | |
WO2002083931A3 (fr) | Procede pour fixer des biomolecules sur des surfaces chimiquement inertes | |
WO2004023104A3 (fr) | Capillaires d'electrophorese interieurement garnis et systeme | |
EP1118659A4 (fr) | Systemes d'expression servant a l'expression d'acides nucleiques fonctionnels |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
AK | Designated contracting states |
Kind code of ref document: A2 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
|
AX | Request for extension of the european patent |
Free format text: AL;LT;LV;MK;RO;SI |
|
PUAL | Search report despatched |
Free format text: ORIGINAL CODE: 0009013 |
|
17P | Request for examination filed |
Effective date: 20010303 |
|
AK | Designated contracting states |
Kind code of ref document: A3 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
|
AX | Request for extension of the european patent |
Free format text: AL;LT;LV;MK;RO;SI |
|
17Q | First examination report despatched |
Effective date: 20011121 |
|
AKX | Designation fees paid |
Free format text: AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
|
GRAH | Despatch of communication of intention to grant a patent |
Free format text: ORIGINAL CODE: EPIDOS IGRA |
|
RTI1 | Title (correction) |
Free format text: PROCESS FOR THE COATING OF CAPILLARIES WITH CROSS-LINKED HYDROPHILIC POLYMERS |
|
GRAS | Grant fee paid |
Free format text: ORIGINAL CODE: EPIDOSNIGR3 |
|
GRAA | (expected) grant |
Free format text: ORIGINAL CODE: 0009210 |
|
AK | Designated contracting states |
Kind code of ref document: B1 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: IT Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT;WARNING: LAPSES OF ITALIAN PATENTS WITH EFFECTIVE DATE BEFORE 2007 MAY HAVE OCCURRED AT ANY TIME BEFORE 2007. THE CORRECT EFFECTIVE DATE MAY BE DIFFERENT FROM THE ONE RECORDED. Effective date: 20030924 Ref country code: FI Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20030924 Ref country code: IE Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20030924 Ref country code: CY Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20030924 |
|
REG | Reference to a national code |
Ref country code: GB Ref legal event code: FG4D Free format text: NOT ENGLISH |
|
REG | Reference to a national code |
Ref country code: CH Ref legal event code: NV Representative=s name: A. BRAUN, BRAUN, HERITIER, ESCHMANN AG PATENTANWAE Ref country code: CH Ref legal event code: EP |
|
REG | Reference to a national code |
Ref country code: SE Ref legal event code: TRGR |
|
REG | Reference to a national code |
Ref country code: IE Ref legal event code: FG4D Free format text: GERMAN |
|
REF | Corresponds to: |
Ref document number: 50003808 Country of ref document: DE Date of ref document: 20031030 Kind code of ref document: P |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: GR Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20031224 Ref country code: DK Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20031224 |
|
GBT | Gb: translation of ep patent filed (gb section 77(6)(a)/1977) |
Effective date: 20031209 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: ES Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20040104 |
|
REG | Reference to a national code |
Ref country code: IE Ref legal event code: FD4D |
|
ET | Fr: translation filed | ||
PLBE | No opposition filed within time limit |
Free format text: ORIGINAL CODE: 0009261 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: LU Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20040803 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: MC Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20040831 |
|
26N | No opposition filed |
Effective date: 20040625 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: SE Payment date: 20050811 Year of fee payment: 6 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: CH Payment date: 20050812 Year of fee payment: 6 Ref country code: NL Payment date: 20050812 Year of fee payment: 6 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: AT Payment date: 20050816 Year of fee payment: 6 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: BE Payment date: 20050914 Year of fee payment: 6 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: AT Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20060803 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: SE Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20060804 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: FR Payment date: 20060817 Year of fee payment: 7 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: LI Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20060831 Ref country code: CH Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20060831 Ref country code: BE Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20060831 |
|
REG | Reference to a national code |
Ref country code: GB Ref legal event code: 732E |
|
REG | Reference to a national code |
Ref country code: FR Ref legal event code: TP |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: NL Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20070301 |
|
REG | Reference to a national code |
Ref country code: CH Ref legal event code: PL |
|
EUG | Se: european patent has lapsed | ||
NLV4 | Nl: lapsed or anulled due to non-payment of the annual fee |
Effective date: 20070301 |
|
BERE | Be: lapsed |
Owner name: STUDIENG.- *KOHLE M.B.H. Effective date: 20060831 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: PT Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20040224 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: GB Payment date: 20070830 Year of fee payment: 8 |
|
REG | Reference to a national code |
Ref country code: FR Ref legal event code: ST Effective date: 20080430 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: FR Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20070831 |
|
GBPC | Gb: european patent ceased through non-payment of renewal fee |
Effective date: 20080803 |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: GB Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20080803 |
|
PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: DE Payment date: 20160726 Year of fee payment: 17 |
|
REG | Reference to a national code |
Ref country code: DE Ref legal event code: R119 Ref document number: 50003808 Country of ref document: DE |
|
PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: DE Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20180301 |