EP1032257A1 - MONOTERPENES SYNTHASES TIREES DE SAPIN GRANDISSIME ($i(Abies grandis)) - Google Patents

MONOTERPENES SYNTHASES TIREES DE SAPIN GRANDISSIME ($i(Abies grandis))

Info

Publication number
EP1032257A1
EP1032257A1 EP98935641A EP98935641A EP1032257A1 EP 1032257 A1 EP1032257 A1 EP 1032257A1 EP 98935641 A EP98935641 A EP 98935641A EP 98935641 A EP98935641 A EP 98935641A EP 1032257 A1 EP1032257 A1 EP 1032257A1
Authority
EP
European Patent Office
Prior art keywords
seq
synthase
gymnosperm
monoteφene
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98935641A
Other languages
German (de)
English (en)
Other versions
EP1032257A4 (fr
Inventor
Joerg Bohlmann
Christopher L. Steele
Rodney B. Croteau
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
Washington State University Research Foundation
Original Assignee
University of Washington
Washington State University Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Washington, Washington State University Research Foundation filed Critical University of Washington
Publication of EP1032257A1 publication Critical patent/EP1032257A1/fr
Publication of EP1032257A4 publication Critical patent/EP1032257A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

On a isolé et séquencé des ADNc codant la myrcène synthase, (-)-limonène synthase et (-)-pinène synthase tirée du sapin grandissime (Abies grandis) et on a déterminé les séquences d'acides aminés correspondantes. Par conséquent, l'invention concerne les séquences d'ADN isolées (SEQ ID NO:1; SEQ ID NO:3 ET SEQ ID NO:5) codant pour l'expresssion de myrcène synthase (SEQ ID NO:2), (-)-pinène synthase (SEQ ID NO:4) et (-)-limonène synthase (SEQ ID NO:6), respectivement, tirées du sapin grandissime (Abies grandis). Dans d'autres aspects, l'invention concerne des véhicules de clonage recombinés réplicables codant pour la myrcène synthase, la (-)-limonène synthase et la (-)-pinène synthase, ou pour une séquence de base suffisamment complémentaire d'au moins une partie d'ADN ou d'ARN de myrcène synthase, de (-)-limonène synthase ou de (-)-pinène synthase afin de permettre une hybridation avec ceux-ci. Dans d'autres aspects, l'invention concerne des cellules hôtes modifiées ayant été transformées, transfectées, infectées et/ou injectées avec un véhicule de clonage recombiné et/ou une séquence d'ADN codant la myrcène synthase, la (-)-limonène synthase ou la (-)-pinène synthase. Ainsi, on obtient des systèmes et des procédés d'expression recombinée de monoterpènes synthases recombinées pré-citées pouvant être utilisées pour faciliter leur reproduction, isolement et purification en quantités significatives. On peut utiliser les myrcène synthase (-)-limonène synthase ou (-)-pinène synthase recombinées pour obtenir une expression ou une expression améliorée de myrcène synthase, (-)-limonène synthase ou (-)-pinène synthase dans des plantes afin d'améliorer la production de monoterpènoïdes, ou on peut également les utiliser à la régulation ou à l'expression de myrcène synthase, (-)-limonène synthase et (-)-pinène synthase ou dans la production de leurs produits.
EP98935641A 1997-07-11 1998-07-10 MONOTERPENES SYNTHASES TIREES DE SAPIN GRANDISSIME ($i(Abies grandis)) Withdrawn EP1032257A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US5224997P 1997-07-11 1997-07-11
US52249P 1997-07-11
PCT/US1998/014528 WO1999002030A1 (fr) 1997-07-11 1998-07-10 MONOTERPENES SYNTHASES TIREES DE SAPIN GRANDISSIME (Abies grandis)

Publications (2)

Publication Number Publication Date
EP1032257A1 true EP1032257A1 (fr) 2000-09-06
EP1032257A4 EP1032257A4 (fr) 2005-03-16

Family

ID=21976364

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98935641A Withdrawn EP1032257A4 (fr) 1997-07-11 1998-07-10 MONOTERPENES SYNTHASES TIREES DE SAPIN GRANDISSIME ($i(Abies grandis))

Country Status (5)

Country Link
EP (1) EP1032257A4 (fr)
AU (1) AU8483998A (fr)
CA (1) CA2296664A1 (fr)
TW (1) TW585918B (fr)
WO (1) WO1999002030A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6265639B1 (en) * 1998-01-22 2001-07-24 Washington State University Foundation Gymnosperm nucleic acid molecules encoding sesquiterpene synthases and methods of use
AR022383A1 (es) 1998-09-18 2002-09-04 Univ Kentucky Res Found Sintasas
AU6238200A (en) * 1999-07-26 2001-02-13 Washington State University Research Foundation Monoterpene synthases from grand fir (abies grandis)
US6818424B2 (en) 2000-09-01 2004-11-16 E. I. Du Pont De Nemours And Company Production of cyclic terpenoids
WO2013110191A1 (fr) 2012-01-23 2013-08-01 The University Of British Columbia Terpénoïdes transporteurs abc et leurs procédés d'utilisation
CA2873405A1 (fr) * 2012-05-11 2013-11-14 Donald Danforth Plant Science Center Procedes de production a haut rendement de terpenes
EP3368673B1 (fr) 2015-10-29 2020-07-29 Amyris, Inc. Compositions et procédés permettant la production de myrcène
EP3878138B1 (fr) * 2018-11-08 2024-05-01 Telefonaktiebolaget LM Ericsson (publ) Dimensionnement de services de réseau (ns)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4948811A (en) * 1988-01-26 1990-08-14 The Procter & Gamble Company Salad/cooking oil balanced for health benefits
CA2118071C (fr) * 1993-10-28 2004-09-14 Rodney B. Croteau Synthase du limonene codant pour l'adn qui provient de mentha spicata

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 2 August 1996 (1996-08-02), STOFFER-VOGEL,B., WILDUNG,M.R., VOGEL,G. AND CROTEAU,R.B.: "Abies grandis abietadiene synthase (ac22) mRNA, complete cds." XP002297128 retrieved from EBI Database accession no. U50768 *
MAU CHRISTOPHER J D ET AL: "Cloning of casbene synthase cDNA: Evidence for conserved structural features among terpenoid cyclases in plants" PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 91, no. 18, 1994, pages 8497-8501, XP002297136 ISSN: 0027-8424 *
See also references of WO9902030A1 *
STEELE CHRISTOPHER L ET AL: "Induced oleoresin biosynthesis in grand fir as a defense against bark beetles" PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 92, no. 10, 1995, pages 4164-4168, XP008035418 ISSN: 0027-8424 *

Also Published As

Publication number Publication date
TW585918B (en) 2004-05-01
CA2296664A1 (fr) 1999-01-21
AU8483998A (en) 1999-02-08
EP1032257A4 (fr) 2005-03-16
WO1999002030A1 (fr) 1999-01-21

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