EP1025113A4 - Improved process for performing polynucleotide separations - Google Patents

Improved process for performing polynucleotide separations

Info

Publication number
EP1025113A4
EP1025113A4 EP98923524A EP98923524A EP1025113A4 EP 1025113 A4 EP1025113 A4 EP 1025113A4 EP 98923524 A EP98923524 A EP 98923524A EP 98923524 A EP98923524 A EP 98923524A EP 1025113 A4 EP1025113 A4 EP 1025113A4
Authority
EP
European Patent Office
Prior art keywords
improved process
performing polynucleotide
polynucleotide separations
separations
improved
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98923524A
Other languages
German (de)
French (fr)
Other versions
EP1025113A1 (en
Inventor
Douglas T Gjerde
Robert M Haefele
David W Togami
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Precipio Inc
Original Assignee
Transgenomic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgenomic Inc filed Critical Transgenomic Inc
Publication of EP1025113A1 publication Critical patent/EP1025113A1/en
Publication of EP1025113A4 publication Critical patent/EP1025113A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/265—Adsorption chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
EP98923524A 1997-06-10 1998-05-19 Improved process for performing polynucleotide separations Withdrawn EP1025113A4 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US4912397P 1997-06-10 1997-06-10
US49123P 1997-06-10
US6383597P 1997-10-30 1997-10-30
US63835P 1997-10-30
PCT/US1998/010228 WO1998056798A1 (en) 1997-06-10 1998-05-19 Improved process for performing polynucleotide separations

Publications (2)

Publication Number Publication Date
EP1025113A1 EP1025113A1 (en) 2000-08-09
EP1025113A4 true EP1025113A4 (en) 2002-01-09

Family

ID=26726874

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98923524A Withdrawn EP1025113A4 (en) 1997-06-10 1998-05-19 Improved process for performing polynucleotide separations

Country Status (5)

Country Link
EP (1) EP1025113A4 (en)
JP (1) JP2001524133A (en)
AU (1) AU743789B2 (en)
CA (1) CA2288813A1 (en)
WO (1) WO1998056798A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7138518B1 (en) 1996-11-13 2006-11-21 Transgenomic, Inc. Liquid chromatographic separation of polynucleotides
US6475388B1 (en) 1996-11-13 2002-11-05 Transgenomic, Inc. Method and system for RNA analysis by matched ion polynucleotide chromatography
US6576133B2 (en) 1996-11-13 2003-06-10 Transgenomic, Inc Method and system for RNA analysis by matched ion polynucleotide chromatography
US6258264B1 (en) 1998-04-10 2001-07-10 Transgenomic, Inc. Non-polar media for polynucleotide separations
WO2001066216A1 (en) * 2000-03-09 2001-09-13 Transgenomic, Inc. Method and system for rna analysis by matched ion polynucleotide chromatography
AU2001259113A1 (en) * 2000-04-21 2001-11-07 Transgenomic, Inc. Apparatus and method for separating and purifying polynucleotides
US6521411B2 (en) * 2000-09-28 2003-02-18 Transgenomic, Inc. Method and system for the preparation of cDNA
AU2001287078A1 (en) * 2000-11-16 2002-05-27 Transgenomic, Inc. Liquid chromatographic separation of polynucleotides
US20020086291A1 (en) * 2000-11-29 2002-07-04 Hornby David P. Methods and reagents for analysis of rna structure and function
AU2002334892A1 (en) 2001-10-05 2003-04-22 Transgenomic, Inc. Methods and compositions for mutation analysis by liquid chromatography
WO2004047981A1 (en) * 2002-11-25 2004-06-10 Varian, Inc. Irregularly-shaped macroporous copolymer particles and methods of using same

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5338448A (en) * 1992-10-16 1994-08-16 Sarasep, Inc. Method of preventing contamination of a chromatography column
US5482628A (en) * 1994-04-15 1996-01-09 Upchurch Scientific, Inc. Column for liquid chromatography

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT398973B (en) * 1992-11-18 1995-02-27 Bonn Guenther Dr METHOD FOR SEPARATING NUCLEIC ACIDS

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5338448A (en) * 1992-10-16 1994-08-16 Sarasep, Inc. Method of preventing contamination of a chromatography column
US5482628A (en) * 1994-04-15 1996-01-09 Upchurch Scientific, Inc. Column for liquid chromatography

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO9856798A1 *

Also Published As

Publication number Publication date
CA2288813A1 (en) 1998-12-17
AU743789B2 (en) 2002-02-07
JP2001524133A (en) 2001-11-27
EP1025113A1 (en) 2000-08-09
WO1998056798A1 (en) 1998-12-17
AU7579698A (en) 1998-12-30

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