AU2001287078A1 - Liquid chromatographic separation of polynucleotides - Google Patents

Liquid chromatographic separation of polynucleotides

Info

Publication number
AU2001287078A1
AU2001287078A1 AU2001287078A AU8707801A AU2001287078A1 AU 2001287078 A1 AU2001287078 A1 AU 2001287078A1 AU 2001287078 A AU2001287078 A AU 2001287078A AU 8707801 A AU8707801 A AU 8707801A AU 2001287078 A1 AU2001287078 A1 AU 2001287078A1
Authority
AU
Australia
Prior art keywords
polynucleotides
chromatographic separation
liquid chromatographic
liquid
separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001287078A
Inventor
Douglas T Gjerde
Robert M. Haefele
Christopher P. Hanna
David Hornby
Alexander I. Kuklin
Paul D. Taylor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Precipio Inc
Original Assignee
Transgenomic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/714,579 external-priority patent/US7138518B1/en
Application filed by Transgenomic Inc filed Critical Transgenomic Inc
Publication of AU2001287078A1 publication Critical patent/AU2001287078A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/10Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/22Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/366Ion-pair, e.g. ion-pair reversed phase
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/42Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424Elution mode
    • B01D15/426Specific type of solvent

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2001287078A 2000-11-16 2001-09-05 Liquid chromatographic separation of polynucleotides Abandoned AU2001287078A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/714,579 US7138518B1 (en) 1996-11-13 2000-11-16 Liquid chromatographic separation of polynucleotides
US09714579 2000-11-16
PCT/US2001/027495 WO2002040130A1 (en) 2000-11-16 2001-09-05 Liquid chromatographic separation of polynucleotides

Publications (1)

Publication Number Publication Date
AU2001287078A1 true AU2001287078A1 (en) 2002-05-27

Family

ID=24870609

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001287078A Abandoned AU2001287078A1 (en) 2000-11-16 2001-09-05 Liquid chromatographic separation of polynucleotides

Country Status (2)

Country Link
AU (1) AU2001287078A1 (en)
WO (1) WO2002040130A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7138518B1 (en) 1996-11-13 2006-11-21 Transgenomic, Inc. Liquid chromatographic separation of polynucleotides
US8586641B2 (en) * 2006-10-19 2013-11-19 Leopold-Franzens-Universitat Innsbruck Monolithic organic copolymer
WO2009103399A2 (en) 2008-02-19 2009-08-27 Merck Patent Gmbh Monolithic supports and methods for their production
EP4070089A1 (en) * 2019-12-05 2022-10-12 Waters Technologies Corporation Chelator based eluents for solid phase extraction of phosphorylated compounds

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6066258A (en) * 1997-12-05 2000-05-23 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
CA2288813A1 (en) * 1997-06-10 1998-12-17 Transgenomic, Inc. Improved process for performing polynucleotide separations
EP1023463A4 (en) * 1997-10-09 2003-01-02 Transgenomic Inc Modifying double stranded dna to enhance separations by matched ion polynucleotide chromatography
WO2001002418A1 (en) * 1999-04-23 2001-01-11 Transgenomic, Inc. Method and system for rna analysis by matched ion polynucleotide chromatography

Also Published As

Publication number Publication date
WO2002040130A1 (en) 2002-05-23

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