WO1998056798A1 - Improved process for performing polynucleotide separations - Google Patents
Improved process for performing polynucleotide separations Download PDFInfo
- Publication number
- WO1998056798A1 WO1998056798A1 PCT/US1998/010228 US9810228W WO9856798A1 WO 1998056798 A1 WO1998056798 A1 WO 1998056798A1 US 9810228 W US9810228 W US 9810228W WO 9856798 A1 WO9856798 A1 WO 9856798A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- eluting solvent
- separation media
- polynucleotide
- solution
- separation
- Prior art date
Links
- 238000000926 separation method Methods 0.000 title claims abstract description 192
- 102000040430 polynucleotide Human genes 0.000 title claims abstract description 116
- 108091033319 polynucleotide Proteins 0.000 title claims abstract description 116
- 239000002157 polynucleotide Substances 0.000 title claims abstract description 116
- 238000000034 method Methods 0.000 title claims abstract description 83
- 230000008569 process Effects 0.000 title description 23
- 239000002904 solvent Substances 0.000 claims abstract description 115
- 239000012634 fragment Substances 0.000 claims abstract description 94
- 150000001768 cations Chemical class 0.000 claims abstract description 93
- 239000011148 porous material Substances 0.000 claims abstract description 61
- 239000000203 mixture Substances 0.000 claims abstract description 53
- 239000000463 material Substances 0.000 claims abstract description 48
- 229910052751 metal Inorganic materials 0.000 claims abstract description 46
- 239000002184 metal Substances 0.000 claims abstract description 46
- 238000010923 batch production Methods 0.000 claims abstract description 27
- 239000011347 resin Substances 0.000 claims abstract description 25
- 229920005989 resin Polymers 0.000 claims abstract description 25
- 239000000356 contaminant Substances 0.000 claims abstract description 20
- 238000004587 chromatography analysis Methods 0.000 claims description 34
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 30
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 24
- 239000010936 titanium Substances 0.000 claims description 24
- 239000002738 chelating agent Substances 0.000 claims description 23
- 229910052719 titanium Inorganic materials 0.000 claims description 23
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- 150000002500 ions Chemical class 0.000 claims description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- MCMNRKCIXSYSNV-UHFFFAOYSA-N Zirconium dioxide Chemical compound O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 claims description 8
- FFUAGWLWBBFQJT-UHFFFAOYSA-N hexamethyldisilazane Chemical compound C[Si](C)(C)N[Si](C)(C)C FFUAGWLWBBFQJT-UHFFFAOYSA-N 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims description 7
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 5
- 239000011260 aqueous acid Substances 0.000 claims description 4
- IJOOHPMOJXWVHK-UHFFFAOYSA-N chlorotrimethylsilane Chemical compound C[Si](C)(C)Cl IJOOHPMOJXWVHK-UHFFFAOYSA-N 0.000 claims description 4
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- 238000011282 treatment Methods 0.000 description 20
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- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 11
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 10
- 238000010828 elution Methods 0.000 description 10
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- 239000002253 acid Substances 0.000 description 9
- 229910052742 iron Inorganic materials 0.000 description 9
- 150000002739 metals Chemical class 0.000 description 9
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- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 4
- 238000010561 standard procedure Methods 0.000 description 4
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
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- 239000005725 8-Hydroxyquinoline Substances 0.000 description 2
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
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- YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical group CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 description 2
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- NNBZCPXTIHJBJL-UHFFFAOYSA-N decalin Chemical compound C1CCCC2CCCCC21 NNBZCPXTIHJBJL-UHFFFAOYSA-N 0.000 description 2
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- 239000003599 detergent Substances 0.000 description 2
- XPPKVPWEQAFLFU-UHFFFAOYSA-N diphosphoric acid Chemical compound OP(O)(=O)OP(O)(O)=O XPPKVPWEQAFLFU-UHFFFAOYSA-N 0.000 description 2
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- 238000002955 isolation Methods 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 229910052755 nonmetal Inorganic materials 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 229960003540 oxyquinoline Drugs 0.000 description 2
- 238000005191 phase separation Methods 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
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- 108091008146 restriction endonucleases Proteins 0.000 description 2
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- 239000000725 suspension Substances 0.000 description 2
- 238000000825 ultraviolet detection Methods 0.000 description 2
- SNUSZUYTMHKCPM-UHFFFAOYSA-N 1-hydroxypyridin-2-one Chemical compound ON1C=CC=CC1=O SNUSZUYTMHKCPM-UHFFFAOYSA-N 0.000 description 1
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
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- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
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- 230000005526 G1 to G0 transition Effects 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
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- 229920006362 Teflon® Polymers 0.000 description 1
- ISWQCIVKKSOKNN-UHFFFAOYSA-L Tiron Chemical compound [Na+].[Na+].OC1=CC(S([O-])(=O)=O)=CC(S([O-])(=O)=O)=C1O ISWQCIVKKSOKNN-UHFFFAOYSA-L 0.000 description 1
- 238000010306 acid treatment Methods 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
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- GDEBSAWXIHEMNF-UHFFFAOYSA-O cupferron Chemical compound [NH4+].O=NN([O-])C1=CC=CC=C1 GDEBSAWXIHEMNF-UHFFFAOYSA-O 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
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- 238000003379 elimination reaction Methods 0.000 description 1
- 239000012156 elution solvent Substances 0.000 description 1
- 229920006351 engineering plastic Polymers 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 208000016861 hereditary angioedema type 3 Diseases 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- VBMVTYDPPZVILR-UHFFFAOYSA-N iron(2+);oxygen(2-) Chemical class [O-2].[Fe+2] VBMVTYDPPZVILR-UHFFFAOYSA-N 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical group OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 125000004430 oxygen atom Chemical group O* 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- GGOZGYRTNQBSSA-UHFFFAOYSA-N pyridine-2,3-diol Chemical group OC1=CC=CN=C1O GGOZGYRTNQBSSA-UHFFFAOYSA-N 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- QEMXHQIAXOOASZ-UHFFFAOYSA-N tetramethylammonium Chemical compound C[N+](C)(C)C QEMXHQIAXOOASZ-UHFFFAOYSA-N 0.000 description 1
- OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 description 1
- 238000006257 total synthesis reaction Methods 0.000 description 1
- 229910052723 transition metal Inorganic materials 0.000 description 1
- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical group CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 1
- PXXNTAGJWPJAGM-UHFFFAOYSA-N vertaline Natural products C1C2C=3C=C(OC)C(OC)=CC=3OC(C=C3)=CC=C3CCC(=O)OC1CC1N2CCCC1 PXXNTAGJWPJAGM-UHFFFAOYSA-N 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
- B01D15/166—Fluid composition conditioning, e.g. gradient
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/265—Adsorption chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP50248799A JP2001524133A (en) | 1997-06-10 | 1998-05-19 | Improved method for performing polynucleotide separation |
EP98923524A EP1025113A4 (en) | 1997-06-10 | 1998-05-19 | Improved process for performing polynucleotide separations |
CA002288813A CA2288813A1 (en) | 1997-06-10 | 1998-05-19 | Improved process for performing polynucleotide separations |
AU75796/98A AU743789B2 (en) | 1997-06-10 | 1998-05-19 | Improved process for performing polynucleotide separations |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US4912397P | 1997-06-10 | 1997-06-10 | |
US60/049,123 | 1997-06-10 | ||
US6383597P | 1997-10-30 | 1997-10-30 | |
US60/063,835 | 1997-10-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1998056798A1 true WO1998056798A1 (en) | 1998-12-17 |
Family
ID=26726874
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1998/010228 WO1998056798A1 (en) | 1997-06-10 | 1998-05-19 | Improved process for performing polynucleotide separations |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1025113A4 (en) |
JP (1) | JP2001524133A (en) |
AU (1) | AU743789B2 (en) |
CA (1) | CA2288813A1 (en) |
WO (1) | WO1998056798A1 (en) |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001066216A1 (en) * | 2000-03-09 | 2001-09-13 | Transgenomic, Inc. | Method and system for rna analysis by matched ion polynucleotide chromatography |
WO2001081566A2 (en) * | 2000-04-21 | 2001-11-01 | Transgenomic, Inc. | Apparatus and method for separating and purifying polynucleotides |
WO2002040130A1 (en) * | 2000-11-16 | 2002-05-23 | Transgenomic, Inc. | Liquid chromatographic separation of polynucleotides |
WO2002061130A2 (en) * | 2000-11-29 | 2002-08-08 | Transgenomic, Inc. | Methods and reagents for analysis of rna structure and function |
US6475388B1 (en) | 1996-11-13 | 2002-11-05 | Transgenomic, Inc. | Method and system for RNA analysis by matched ion polynucleotide chromatography |
US6521411B2 (en) * | 2000-09-28 | 2003-02-18 | Transgenomic, Inc. | Method and system for the preparation of cDNA |
US6524480B2 (en) | 1997-12-05 | 2003-02-25 | Transgenomic, Inc. | Non-polar media for polynucleotide separations |
US6576133B2 (en) | 1996-11-13 | 2003-06-10 | Transgenomic, Inc | Method and system for RNA analysis by matched ion polynucleotide chromatography |
WO2004047981A1 (en) * | 2002-11-25 | 2004-06-10 | Varian, Inc. | Irregularly-shaped macroporous copolymer particles and methods of using same |
US7135289B2 (en) | 2001-10-05 | 2006-11-14 | Transgenomic, Inc. | Methods and compositions for mutation analysis of polynucleotides by liquid chromatography |
US7138518B1 (en) | 1996-11-13 | 2006-11-21 | Transgenomic, Inc. | Liquid chromatographic separation of polynucleotides |
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- 1998-05-19 WO PCT/US1998/010228 patent/WO1998056798A1/en not_active Application Discontinuation
- 1998-05-19 AU AU75796/98A patent/AU743789B2/en not_active Ceased
- 1998-05-19 JP JP50248799A patent/JP2001524133A/en active Pending
- 1998-05-19 CA CA002288813A patent/CA2288813A1/en not_active Abandoned
- 1998-05-19 EP EP98923524A patent/EP1025113A4/en not_active Withdrawn
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US5585236A (en) * | 1992-11-18 | 1996-12-17 | Sarasep, Inc. | Nucleic acid separation on alkylated nonporous polymer beads |
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ERIKSSON S., ET AL.: "SEPARATION OF DNA RESTRICTION FRAGMENTS BY ION-PAIR CHROMATOGRAPHY.", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V., NL, vol. 359., 1 January 1986 (1986-01-01), NL, pages 265 - 274., XP002910334, ISSN: 0021-9673, DOI: 10.1016/0021-9673(86)80080-2 * |
HUBER C. G., ET AL.: "HIGH-RESOLUTION LIQUID CHROMATOGRAPHY OF DNA FRAGMENTS ON NON- POROUS POLY(STYRENE-DIVINYLBENZENE) PARTICLES.", NUCLEIC ACIDS RESEARCH, INFORMATION RETRIEVAL LTD., GB, vol. 21., no. 05., 1 January 1993 (1993-01-01), GB, pages 1061 - 1066., XP002910335, ISSN: 0305-1048 * |
See also references of EP1025113A4 * |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7138518B1 (en) | 1996-11-13 | 2006-11-21 | Transgenomic, Inc. | Liquid chromatographic separation of polynucleotides |
US6576133B2 (en) | 1996-11-13 | 2003-06-10 | Transgenomic, Inc | Method and system for RNA analysis by matched ion polynucleotide chromatography |
US6475388B1 (en) | 1996-11-13 | 2002-11-05 | Transgenomic, Inc. | Method and system for RNA analysis by matched ion polynucleotide chromatography |
US6524480B2 (en) | 1997-12-05 | 2003-02-25 | Transgenomic, Inc. | Non-polar media for polynucleotide separations |
WO2001066216A1 (en) * | 2000-03-09 | 2001-09-13 | Transgenomic, Inc. | Method and system for rna analysis by matched ion polynucleotide chromatography |
WO2001081566A2 (en) * | 2000-04-21 | 2001-11-01 | Transgenomic, Inc. | Apparatus and method for separating and purifying polynucleotides |
WO2001081566A3 (en) * | 2000-04-21 | 2002-05-10 | Transgenomic Inc | Apparatus and method for separating and purifying polynucleotides |
US6521411B2 (en) * | 2000-09-28 | 2003-02-18 | Transgenomic, Inc. | Method and system for the preparation of cDNA |
WO2002040130A1 (en) * | 2000-11-16 | 2002-05-23 | Transgenomic, Inc. | Liquid chromatographic separation of polynucleotides |
WO2002061130A3 (en) * | 2000-11-29 | 2003-05-15 | Transgenomic Inc | Methods and reagents for analysis of rna structure and function |
WO2002061130A2 (en) * | 2000-11-29 | 2002-08-08 | Transgenomic, Inc. | Methods and reagents for analysis of rna structure and function |
US7135289B2 (en) | 2001-10-05 | 2006-11-14 | Transgenomic, Inc. | Methods and compositions for mutation analysis of polynucleotides by liquid chromatography |
WO2004047981A1 (en) * | 2002-11-25 | 2004-06-10 | Varian, Inc. | Irregularly-shaped macroporous copolymer particles and methods of using same |
Also Published As
Publication number | Publication date |
---|---|
JP2001524133A (en) | 2001-11-27 |
EP1025113A1 (en) | 2000-08-09 |
CA2288813A1 (en) | 1998-12-17 |
EP1025113A4 (en) | 2002-01-09 |
AU743789B2 (en) | 2002-02-07 |
AU7579698A (en) | 1998-12-30 |
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