EP1017850A2 - Sequences genetiques, procedes et dispositifs de diagnostic et/ou de quantification pour l'identification de souches de staphylocoques - Google Patents
Sequences genetiques, procedes et dispositifs de diagnostic et/ou de quantification pour l'identification de souches de staphylocoquesInfo
- Publication number
- EP1017850A2 EP1017850A2 EP98944907A EP98944907A EP1017850A2 EP 1017850 A2 EP1017850 A2 EP 1017850A2 EP 98944907 A EP98944907 A EP 98944907A EP 98944907 A EP98944907 A EP 98944907A EP 1017850 A2 EP1017850 A2 EP 1017850A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- nucleotide sequence
- sequence
- base pairs
- fema
- genetic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241000295644 Staphylococcaceae Species 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000002068 genetic effect Effects 0.000 title claims description 15
- 238000011002 quantification Methods 0.000 title claims description 12
- 239000002773 nucleotide Substances 0.000 claims abstract description 53
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 53
- 108091034117 Oligonucleotide Proteins 0.000 claims abstract description 32
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 35
- 238000009396 hybridization Methods 0.000 claims description 19
- 239000000523 sample Substances 0.000 claims description 16
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 11
- 230000003321 amplification Effects 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 9
- 239000003242 anti bacterial agent Substances 0.000 claims description 8
- 229940088710 antibiotic agent Drugs 0.000 claims description 8
- 230000000052 comparative effect Effects 0.000 claims description 4
- 238000000636 Northern blotting Methods 0.000 claims description 2
- 238000002105 Southern blotting Methods 0.000 claims description 2
- 238000011065 in-situ storage Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 10
- 230000000155 isotopic effect Effects 0.000 claims 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract description 11
- 230000000295 complement effect Effects 0.000 abstract description 6
- 101150022703 femA gene Proteins 0.000 description 39
- 241000894007 species Species 0.000 description 13
- 241000191963 Staphylococcus epidermidis Species 0.000 description 11
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- 108091035707 Consensus sequence Proteins 0.000 description 6
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- 150000007523 nucleic acids Chemical group 0.000 description 6
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- 238000012163 sequencing technique Methods 0.000 description 5
- 108091093088 Amplicon Proteins 0.000 description 4
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 description 4
- 206010041925 Staphylococcal infections Diseases 0.000 description 4
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- 241001134656 Staphylococcus lugdunensis Species 0.000 description 4
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- 241000588724 Escherichia coli Species 0.000 description 3
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- 108010013639 Peptidoglycan Proteins 0.000 description 3
- 241001147736 Staphylococcus capitis Species 0.000 description 3
- 241000191973 Staphylococcus xylosus Species 0.000 description 3
- 150000001413 amino acids Chemical group 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000003748 differential diagnosis Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 101150084423 femB gene Proteins 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- 238000007403 mPCR Methods 0.000 description 3
- 101150008979 mecA gene Proteins 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- 101100502610 Caenorhabditis elegans fem-2 gene Proteins 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 101710202686 Penicillin-sensitive transpeptidase Proteins 0.000 description 2
- 108700005075 Regulator Genes Proteins 0.000 description 2
- 241000192099 Staphylococcus schleiferi Species 0.000 description 2
- 241000192097 Staphylococcus sciuri Species 0.000 description 2
- 241000191978 Staphylococcus simulans Species 0.000 description 2
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- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
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- 206010011409 Cross infection Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
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- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
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- MXHCPCSDRGLRER-UHFFFAOYSA-N pentaglycine Chemical group NCC(=O)NCC(=O)NCC(=O)NCC(=O)NCC(O)=O MXHCPCSDRGLRER-UHFFFAOYSA-N 0.000 description 1
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- 229960003165 vancomycin Drugs 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Abstract
L'invention se rapporte à des oligonucléotides pour l'identification spécifique d'espèces de staphylocoques dont la séquence nucléotide présente entre 15 et 350 paires de base, de préférence entre 15 et 45 paires de base, obtenues par 'consensus' de la séquence nucléotide femA (CNS) de la figure ou de son brin complémentaire. L'invention concerne également un procédé et un dispositif de diagnostic utilisant lesdits oligonucléotides pour l'identification de divers types de souches d'espèces de staphylocoques.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP98944907A EP1017850A2 (fr) | 1997-09-26 | 1998-09-28 | Sequences genetiques, procedes et dispositifs de diagnostic et/ou de quantification pour l'identification de souches de staphylocoques |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP97870146 | 1997-09-26 | ||
EP97870146 | 1997-09-26 | ||
PCT/BE1998/000141 WO1999016780A2 (fr) | 1997-09-26 | 1998-09-28 | Sequences genetiques, procedes et dispositifs de diagnostic et/ou de quantification pour l'identification de souches de staphylocoques |
EP98944907A EP1017850A2 (fr) | 1997-09-26 | 1998-09-28 | Sequences genetiques, procedes et dispositifs de diagnostic et/ou de quantification pour l'identification de souches de staphylocoques |
Publications (1)
Publication Number | Publication Date |
---|---|
EP1017850A2 true EP1017850A2 (fr) | 2000-07-12 |
Family
ID=8231045
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP98944907A Withdrawn EP1017850A2 (fr) | 1997-09-26 | 1998-09-28 | Sequences genetiques, procedes et dispositifs de diagnostic et/ou de quantification pour l'identification de souches de staphylocoques |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1017850A2 (fr) |
JP (1) | JP2001518283A (fr) |
CA (1) | CA2301285A1 (fr) |
WO (1) | WO1999016780A2 (fr) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1096024A1 (fr) * | 1999-10-28 | 2001-05-02 | Remacle, José | Méthode et trousse pour le criblage et/ou la quantification de séquences d' acides nucléiques multiples homologues avec des ensembles de sondes |
EP1136566A1 (fr) * | 2000-03-24 | 2001-09-26 | Facultés Universitaires Notre-Dame de la Paix | Procédé et trousse pour la détection et/ou la quantification d'acides nucléiques homologues par un ensemble d'ADN |
US7829313B2 (en) | 2000-03-24 | 2010-11-09 | Eppendorf Array Technologies | Identification and quantification of a plurality of biological (micro)organisms or their components |
US7205104B2 (en) | 2000-03-24 | 2007-04-17 | Eppendorf Array Technologies Sa (Eat) | Identification of biological (micro) organisms by detection of their homologous nucleotide sequences on arrays |
US7202026B2 (en) * | 2000-03-24 | 2007-04-10 | Eppendorf Array Technologies Sa (Eat) | Identification of a large number of biological (micro)organisms groups at different levels by their detection on a same array |
US7875442B2 (en) | 2000-03-24 | 2011-01-25 | Eppendorf Array Technologies | Identification and quantification of a plurality of biological (micro)organisms or their components |
US7090997B2 (en) * | 2000-10-26 | 2006-08-15 | Eisai Co., Ltd. | Diagnostic agent and test method for colon cancer using tannase as index |
US7666588B2 (en) | 2001-03-02 | 2010-02-23 | Ibis Biosciences, Inc. | Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy |
AU2013231102B2 (en) * | 2001-03-02 | 2016-04-28 | Ibis Biosciences, Inc. | Methods for rapid identification of pathogens in humans and animals |
WO2004060278A2 (fr) | 2002-12-06 | 2004-07-22 | Isis Pharmaceuticals, Inc. | Procedes d'identification rapide de pathogenes chez l'homme et les betes |
AU2002312764A1 (en) * | 2001-03-15 | 2002-10-21 | Schrenzel, Jacques | Detection of methicillin-resistant staphylococcus aureus (mrsa) |
CA2875478A1 (fr) | 2003-05-13 | 2005-02-24 | Gen-Probe Incorporated | Procede et trousse permettant l'identification de micro-organismes resistants aux antibiotiques |
US7338763B2 (en) | 2004-06-02 | 2008-03-04 | Eppendorf Array Technologies S.A. | Method and kit for the detection and/or quantification of homologous nucleotide sequences on arrays |
CN111183223A (zh) * | 2017-10-12 | 2020-05-19 | 三井化学株式会社 | mecA基因扩增用引物对、mecA基因检测试剂盒及mecA基因检测方法 |
CN112195225A (zh) * | 2020-10-14 | 2021-01-08 | 南通大学附属医院 | 基于耐药基因MecA检测耐甲氧西林葡萄球菌的方法 |
WO2022253743A1 (fr) | 2021-06-02 | 2022-12-08 | Syngulon S.A. | Bactériocine pour une nouvelle application |
EP4346869A1 (fr) | 2021-06-02 | 2024-04-10 | Syngulon S.A. | Bactériocine de céréine 7b pour une nouvelle application |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2075423A1 (fr) * | 1991-08-13 | 1993-02-14 | Paul Luther Skatrud | Methode de detection rapide des staphylocoques resistant a la methicilline |
EP0625575A3 (fr) * | 1993-04-30 | 1995-02-22 | Lilly Co Eli | Gène Fem A de staphylococcus épidermis, protéine Fem A, et vecteurs et micro-organismes contenant le gène Fem A. |
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1998
- 1998-09-28 JP JP2000513862A patent/JP2001518283A/ja active Pending
- 1998-09-28 CA CA002301285A patent/CA2301285A1/fr not_active Abandoned
- 1998-09-28 WO PCT/BE1998/000141 patent/WO1999016780A2/fr not_active Application Discontinuation
- 1998-09-28 EP EP98944907A patent/EP1017850A2/fr not_active Withdrawn
Non-Patent Citations (1)
Title |
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See references of WO9916780A2 * |
Also Published As
Publication number | Publication date |
---|---|
WO1999016780A2 (fr) | 1999-04-08 |
JP2001518283A (ja) | 2001-10-16 |
WO1999016780A3 (fr) | 1999-08-05 |
CA2301285A1 (fr) | 1999-04-08 |
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