EP0993586B1 - Drying module - Google Patents

Drying module Download PDF

Info

Publication number
EP0993586B1
EP0993586B1 EP98940128A EP98940128A EP0993586B1 EP 0993586 B1 EP0993586 B1 EP 0993586B1 EP 98940128 A EP98940128 A EP 98940128A EP 98940128 A EP98940128 A EP 98940128A EP 0993586 B1 EP0993586 B1 EP 0993586B1
Authority
EP
European Patent Office
Prior art keywords
dryer module
filter support
module according
filter
heating system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP98940128A
Other languages
German (de)
French (fr)
Other versions
EP0993586A1 (en
Inventor
Helmut BLÖCKER
Gerhard Kauer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Original Assignee
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helmholtz Zentrum fuer Infektionsforschung HZI GmbH filed Critical Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Publication of EP0993586A1 publication Critical patent/EP0993586A1/en
Application granted granted Critical
Publication of EP0993586B1 publication Critical patent/EP0993586B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26—DRYING
    • F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B9/00—Machines or apparatus for drying solid materials or objects at rest or with only local agitation; Domestic airing cupboards
    • F26B9/003—Small self-contained devices, e.g. portable
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26—DRYING
    • F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B21/00—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26—DRYING
    • F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B9/00—Machines or apparatus for drying solid materials or objects at rest or with only local agitation; Domestic airing cupboards
    • F26B9/06—Machines or apparatus for drying solid materials or objects at rest or with only local agitation; Domestic airing cupboards in stationary drums or chambers

Definitions

  • the invention relates to a dryer module for drying of filter carriers, such as those used in DNA preparation be used.
  • the preparation protocol described by Qiagen involves washing the one in filter elements Filter carrier adsorbed DNA with ethanol-containing buffer. Experiments have shown that traces of ethanol are the result of subsequent sequencing reactions drastically deteriorate. The enzymes involved will probably inhibited by ethanol. In this step first the larger amounts of ethanol from the filters removed in a known manner. This can happen with a Implementation of the protocol, for example in a the corresponding vacuum apparatus that the applied vacuum is applied longer, than would be necessary to remove the washing liquid. The air flow generated in the filter in this way is removed the larger amounts of ethanol from the filters.
  • the invention is therefore based on the object To create device with which the remaining Residual alcohol is removed from filter media as they are for example in the Qiagen protocol.
  • the invention relates to a dryer module for drying a filter carrier in the context of DNA preparation with a heater and a fan element, the heater being arranged downstream of the fan element and the filters arranged in the filter carrier being blown and dried from below, characterized, that the filters are arranged hanging in the filter carrier, and the filter carrier has spacer sleeves which can be inserted into corresponding centering shafts of a centering plate for receiving the filter carrier, heating wires running parallel to a rectangular cross section of an air shaft being arranged upstream of the centering plate as a heater and the dryer module has a tangential fan as the fan element.
  • the dryer module is characterized in that the fan element is driven by an electric motor.
  • the dryer module is characterized in that a nozzle for concentrating the air flow is arranged between the air shaft and the centering element for the filter carrier.
  • the dryer module is characterized in that the dryer module is arranged in an opening of a table top.
  • the dryer module is characterized in that the centering plate for receiving the filter carrier is arranged on support elements.
  • the dryer module is characterized in that the module has a temperature sensor that the module has an electrical controller for controlling the at least one motor and the heater, the heater being controlled as a function of the temperature measured by the temperature sensor, and that the controller is provided with a delay circuit which, in the event that the dryer module is switched off, causes the at least one fan element (1) to be switched off in time after the heating (5) is switched off.
  • the invention also provides the use of the dryer module in an automated environment as well as a stand-alone device.
  • the step of removing the Ethanol from the area between the outlet nozzle and Plastic sheathing of the filter carrier not manually performed, but automated. Blows for this purpose the dryer module heated air (maximum 50 ° C) from below evaporated against the filter support and the ethanol. The on this way dried filter is again in a Vacuum chamber placed, then again for a certain Time to be ventilated by applying a vacuum. In This second step will leave traces of ethanol carried on. Finally, this is how it is treated Filter placed on the dryer module again to pass through to ensure that the temperature-controlled air flow traces of ethanol still present for good be evaporated. Only then will the filter holder for example put back in the pipetting robot, to the eluent for the release of the DNA from the Pipette in filters.
  • two tangential fans 1 are coupled on their long sides in order to obtain an air flow that is as homogeneous as possible. This results in a uniform air flow over the entire surface of the filter carrier to be dried (not shown).
  • the air flow is a maximum of 2x80 m 3 / h and can be regulated via a series transformer (not shown) via the rotational speed of the motors 3 of the pair of tangential fans 1.
  • the rectangular duct 4 is constructed so that the Air flow, like a nozzle 6, towards the outlet opening is bundled. It is made of 2 mm thick iron sheet manufactured and soldered to a closed shape. On in the lower area there is a holder for one Heating 5. Below are the two tangential fans 1 attached. The upper part of the air duct 4 is in one correspondingly dimensioned recess in a table top 7 attached and thus in the work area at precisely defined Fixed position. The heating elements 5a, 5b and Fan elements 1 are located under this arrangement the work table 7.
  • the opening of the air shaft 4 is preferably at ground level with the work surface of a Robot tables (not shown) in case of use of the dryer module in an automated process.
  • the heater 5 is in the preferred embodiment from two heating elements 5a, 5b, as shown in FIGS. 2 and 3 will become clearer. These heating elements 5a, 5b are above the outlet opening of the tangential fan 1 attached. Two Multimicalochplatten are each considered Carrier for a heating wire used in coils of about 3 mm distance is clamped in the air flow and this heats it evenly. The coils are there so that the reverse loop outside the Airflow comes to rest, the parallel Heating wires, on the other hand, are perpendicular to the multi-micro plates are arranged.
  • the filter stand consisting of a mounting element 11 and support members 10 is designed so that it centering elements (shown in Fig. 3) on its Contact points to the filter carrier (not shown) having. In particular, 30 ° phases are on the Contact areas to the spacer sleeves (see Fig. 4) to name a filter carrier.
  • the filter stand includes in the preferred embodiment one as a receiving element acting upper support plate or centering plate 10, which the centering elements, namely cutouts for the Spacer sleeves and the contact surface for the edge of the Has filter carrier. On this centering plate are in attached the four corner points 4.5 cm high support elements 10, which each have a fastening bolt at their lower end 8 of 0.3 cm.
  • the control of the fan elements 1 and the The heating elements 5a, 5b are activated separately via a controller interface. So after finishing of the drying process, the heater 5 is first switched off be, so that after a time delay the now cooling the heating wires acting ventilation is stopped.
  • Temperature measurement is a lateral one in the nozzle 6 Recess 13 for receiving a temperature sensor intended.
  • the heating elements 5a, 5b is a Temperature threshold switching controlled.
  • FIG. 2 also shows the dryer module according to the invention in a cross-sectional view, but opposite the 1 of 90 ° around the vertical axis of symmetry is rotated.
  • the two can be seen in parallel arranged tangential fan rollers 1 by corresponding motors 3 are driven.
  • Air duct 4 are the two heating elements 5a, 5b Heater 5 arranged in parallel and almost abutting.
  • the nozzle 6 is part of the shaft 4 designed to bundle the drying air.
  • the dryer module in the opening of a table top 7 or the like attached.
  • a top 8 preferably made of acrylic or a similar material, arranged the Filter support stand consisting of the support elements 9 and the centering plate 11 carries, the filter support stand fixed by means of fastening bolts 9 in the plate 8 and is aligned.
  • FIG. 3 shows the dryer module described above in one View from above.
  • the plate 8 made of acrylic exists and is therefore transparent.
  • Two are shown Tangential fan 1 with drive connections 2 and motors 3, the heating elements 5a and 5b in the air shaft 4, the one Nozzle 6 forms.
  • the centering plate 11 has four Centering shaft 12 for receiving appropriately shaped Spacer sleeves of the filter carrier, which in FIG. 4 are shown. Through these centering shafts 12 alignment of the filter carrier is forced.
  • Fig. 4 shows a cross section through for explanation a commonly used filter carrier 14, the a plurality of filter elements 15 is formed, the are connected to each other in a known manner.
  • the filter carrier usually has a top view rectangular shape.
  • the outer filter elements 15 of the Filter carrier 14 have spacer sleeves 17 which for Centering of the filter carrier 14 in an automated Serve environment. Here they are used for centering in the Centering shafts 12 of the centering plate 11. Further points the filter carrier 14 has a circumferential shape formed as a collar Wall 18 on.

Landscapes

  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Drying Of Solid Materials (AREA)
  • Inorganic Insulating Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A dryer module for drying filter supports in DNA preparation comprises a receiving member for receiving the filter support and at least one blower member, the blower member being arranged below the receiving member for receiving the filter support so that the filter support is blown on an dried from below. The dryer module also has a heating system for warming the air blown from below against the filter support, the heating system being arranged above the blower member, of which there is at least one. The dryer module is electronically controlled by a control means as a function of the measured temperature of the drying air. The dryer module can be used as a stand-along apparatus or in an automated environment.

Description

Die Erfindung betrifft ein Trocknermodul zur Trocknung von Filterträgern, wie sie bei der DNA-Präparation verwendet werden.The invention relates to a dryer module for drying of filter carriers, such as those used in DNA preparation be used.

Bei modernen DNA-Präparationen, deren Produkte zu Sequenzieraufgaben eingesetzt werden sollen, ist eine Automatisierung der arbeitsintensiven manuellen Schritte unabdingbar, was insbesondere bei den neueren Megasequenzierprojekten deutlich wird (Human Genome Project). Während früher keine sogenannten Fertig-Kits zur Verfügung standen, welche die Aufarbeitung einklonierter DNA-Sequenzen vereinfachten, existieren heute bereits. einige kommerziell verfügbare Hilfsmittel, die sich auch über Roboter zur automatischen Abarbeitung der entsprechenden Protokolle eignen (wie beispielsweise dem weitverbreiteten Qiagen-Protokoll).With modern DNA preparations, their products too Sequencing tasks to be used is one Automation of labor-intensive manual steps indispensable, especially with the newer ones Megasequencing projects (Human Genome Project). While there were no so-called ready-made kits in the past Were available, which cloned the processing Simplified DNA sequences already exist today. some commercially available aids, too via robots for automatic processing of the appropriate protocols (such as the widespread Qiagen protocol).

Das von Qiagen beschriebene Präparationsprotokoll beinhaltet das Waschen der in Filterelementen eines Filterträgers adsorbierten DNA mit ethanolhaltigem Puffer. Versuche zeigten, daß Spuren von Ethanol das Ergebnis der nachfolgenden Sequenzierreaktionen drastisch verschlechtern. Die beteiligten Enzyme werden wahrscheinlich von Ethanol inhibiert. In diesem Schritt werden zunächst die größeren Mengen Ethanol aus den Filtern auf bekannte Weise entfernt. Dies kann bei einer Durchführung des Protokolls beispielsweise in einer entsprechenden Vakuumapparatur dadurch bewerkstelligt werden, daß das anliegende Vakuum länger appliziert wird, als dies zur Beseitung der Waschflüssigkeit notwendig wäre. Der auf diese Weise im Filter erzeugte Luftstrom entfernt die größeren Mengen Ethanol aus den Filtern. Die besondere Konstruktion der Qiagenfilterträger verunreinigt jedoch den nachfolgenden Sequenzierprozeß, wenn nicht besondere Vorkehrungen getroffen werden, da Ethanolreste an den Wandungen zwischen den Auslaßdüsen und den Kunststoffummantelungen an der Unterseite des Filterträgers verbleiben. Es konnte weiter beobachtet werden, daß eine Verunreinigung durch Ethanol während der Elution der DNA aus dem Filtermaterial in folgender Weise stattfindet: das Eluat bildet während des Absaugens in den Eluatfängern einen Tropfen an der Auslaßdüse. Dieser Tropfen "wandert" vor seinem Abreißen an den Düsen hoch und berührt die sich dort noch befindliche ethanolhaltige Flüssigkeit. Es findet folglich eine Durchmischung statt. Der Tropfen reißt dann ab und fällt in den Eluatfänger. Somit ist eine generelle Verunreinigung des Eluats mit Ethanol gegeben, die so groß ist, daß eine "Vergiftung". des Sequenzieransatzes vorliegt. Der Stand der Technik zeigt bereits ein Trocknermodul zum Trocknen von Produkten, wobei das Trocknermodul ein Aufnahmeelement zur Aufnahme des Produktes und mindestens ein Lüfterelement aufweist, und das Lüfterelement unterhalb des Aufnahmeelementes zur Aufnahme des Produktes angeordnet ist, so daß das Produkt von unten angeblasen und getrocknet wird; vgl. bspw. US 4 812 621, DE 94 01 195, US 3 007 256 und DE 97 363. The preparation protocol described by Qiagen involves washing the one in filter elements Filter carrier adsorbed DNA with ethanol-containing buffer. Experiments have shown that traces of ethanol are the result of subsequent sequencing reactions drastically deteriorate. The enzymes involved will probably inhibited by ethanol. In this step first the larger amounts of ethanol from the filters removed in a known manner. This can happen with a Implementation of the protocol, for example in a the corresponding vacuum apparatus that the applied vacuum is applied longer, than would be necessary to remove the washing liquid. The air flow generated in the filter in this way is removed the larger amounts of ethanol from the filters. The special one However, the construction of the Qiagen filter carrier contaminates the subsequent sequencing process, if not special Precautions are taken as ethanol remains on the Walls between the outlet nozzles and the Plastic sheathing on the underside of the filter holder remain. It was also observed that a Contamination by ethanol during the elution of the DNA from the filter material takes place in the following way: the Eluate forms in the eluate catchers during aspiration a drop on the outlet nozzle. This drop "wanders" before tearing it off at the nozzles and touching them liquid containing ethanol still there. It takes place consequently, mixing takes place. The drop then tears and falls into the eluate. So is a general one Contamination of the eluate with ethanol is given that large is that "poisoning". of the sequencing approach. The prior art already shows a dryer module for drying of products, the dryer module being a receiving element for Product and has at least one fan element, and the fan element below the receiving element for receiving of the product is arranged so that the product from below is blown and dried; see. for example US 4,812,621, DE 94 01 195, US 3,007,256 and DE 97 363.

Der Erfindung liegt nun daher die Aufgabe zugrunde, eine Vorrichtung zu schaffen, mit der der verbleibende Restalkohol von Filterträgern entfernt wird, wie sie beispielsweise in dem Qiagenprotokoll verwendet werden.The invention is therefore based on the object To create device with which the remaining Residual alcohol is removed from filter media as they are for example in the Qiagen protocol.

Die Aufgabe wird durch die Merkmale des Anspruchs 1 gelöst. Vorteilhafte Ausgestaltungen der Erfindung sind Gegenstand der Unteransprüche.The object is achieved by the features of claim 1 solved. Advantageous embodiments of the invention are Subject of the subclaims.

Die Erfindung betrifft ein Trocknermodul zum Trocknen eines Filterträgers im Rahmen der DNA-Präparation mit einer Heizung und einem Lüfterelement, wobei die Heizung stromabwärts vom Lüfterelement angeordnet ist und die in dem Filterträger angeordneten Filter von unten angeblasen und getrocknet werden,
dadurch gekennzeichnet,
daß die Filter in dem Filterträger hängend angeordnet sind, und der Filterträger Abstandshülsen aufweist, die in entsprechende Zentrierschächte einer Zentrierplatte zur Aufnahme des Filterträgers einführbar sind, wobei als Heizung parallel über einen rechteckigen Querschnitt eines Luftschachtes verlaufende Heizdrähte senkrecht zum Luftstrom stromaufwärts der Zentrierplatte angeordnet sind und das Trocknermodul als Lüfterelement einen Tangentiallüfter aufweist. The invention relates to a dryer module for drying a filter carrier in the context of DNA preparation with a heater and a fan element, the heater being arranged downstream of the fan element and the filters arranged in the filter carrier being blown and dried from below,
characterized,
that the filters are arranged hanging in the filter carrier, and the filter carrier has spacer sleeves which can be inserted into corresponding centering shafts of a centering plate for receiving the filter carrier, heating wires running parallel to a rectangular cross section of an air shaft being arranged upstream of the centering plate as a heater and the dryer module has a tangential fan as the fan element.

Nach einer Ausführungsform der Erfindung ist das Trocknermodul dadurch gekennzeichnet, daß das Lüfterelement durch einen elektrischen Motor angetrieben wird.According to one embodiment of the invention, the dryer module is characterized in that the fan element is driven by an electric motor.

Nach einer Ausführungsform der Erfindung ist das Trocknermodul dadurch gekennzeichnet, daß zwischen dem Luftschacht und dem Zentrierelement für den Filterträger eine Düse zur Bündelung des Luftstromes angeordnet ist.According to one embodiment of the invention, the dryer module is characterized in that a nozzle for concentrating the air flow is arranged between the air shaft and the centering element for the filter carrier.

Nach einer Ausführungsform der Erfindung ist das Trocknermodul dadurch gekennzeichnet, daß das Trocknermodul in einer Öffnung einer Tischplatte angeordnet ist.According to one embodiment of the invention, the dryer module is characterized in that the dryer module is arranged in an opening of a table top.

Nach einer Ausführungsform der Erfindung ist das Trocknermodul dadurch gekennzeichnet, daß die Zentrierplatte zur Aufnahme des Filterträgers auf Stützelementen angeordnet ist.According to one embodiment of the invention, the dryer module is characterized in that the centering plate for receiving the filter carrier is arranged on support elements.

Nach einer Ausführungsform der Erfindung ist das Trocknermodul dadurch gekennzeichnet, daß das Modul einen Temperaturfühler aufweist, daß
das Modul eine elektrische Steuerung zur Steuerung des mindestens einen Motors und der Heizung aufweist, wobei die Heizung als Funktion der vom Temperaturfühler gemessenen Temperatur gesteuert wird, und
daß die Steuerung mit einer Verzögerungsschaltung versehen ist, die bewirkt, daß im Falle eines Abschaltens des Trocknermoduls das mindestens eine Lüfterelement (1) zeitlich nach dem Abschalten der Heizung (5) abgeschaltet wird.According to one embodiment of the invention, the dryer module is characterized in that the module has a temperature sensor that
the module has an electrical controller for controlling the at least one motor and the heater, the heater being controlled as a function of the temperature measured by the temperature sensor, and
that the controller is provided with a delay circuit which, in the event that the dryer module is switched off, causes the at least one fan element (1) to be switched off in time after the heating (5) is switched off.

Die Erfindung gibt ferner die Verwendung des Trocknermoduls in einer automatisierten Umgebung sowie als alleinstehendes Gerätan. The invention also provides the use of the dryer module in an automated environment as well as a stand-alone device.

Vorteilhafterweise wird der Schritt des Entfernens des Ethanols aus dem Bereich zwischen Auslaßdüse und Kunststoffummantelung der Filterträger nicht manuell durchgeführt, sondern automatisiert. Zu diesem Zwecke bläst das Trocknermodul erwärmte Luft (maximal 50 °C) von unten gegen den Filterträger und das Ethanol verdampft. Der auf diese Weise getrocknete Filter wird erneut in eine Vakuumkammer gestellt, um dann nochmals für eine gewisse Zeit durch ein angelegtes Vakuum belüftet zu werden. In diesem zweiten Schritt werden verbliebene Ethanolspuren weiter ausgetragen. Abschließend wird das so behandelte Filter nochmals auf das Trocknermodul gebracht, um durch den temperierten Luftstrom sicherzustellen, daß eventuell immer noch vorliegende Spuren von Ethanol endgültig abgedampft werden. Erst dann wird der Filterträger beispielsweise wieder in den Pipettierroboter eingestellt, um das Elutionsmittel für die Freisetzung der DNA aus den Filtern einzupipettieren.Advantageously, the step of removing the Ethanol from the area between the outlet nozzle and Plastic sheathing of the filter carrier not manually performed, but automated. Blows for this purpose the dryer module heated air (maximum 50 ° C) from below evaporated against the filter support and the ethanol. The on this way dried filter is again in a Vacuum chamber placed, then again for a certain Time to be ventilated by applying a vacuum. In This second step will leave traces of ethanol carried on. Finally, this is how it is treated Filter placed on the dryer module again to pass through to ensure that the temperature-controlled air flow traces of ethanol still present for good be evaporated. Only then will the filter holder for example put back in the pipetting robot, to the eluent for the release of the DNA from the Pipette in filters.

Eine bevorzugte Ausführungsform der Erfindung wird nachfolgend anhand der Zeichnungen beschrieben, in denen

  • Fig. 1 eine schematische Querschnittsansicht durch ein in einer Tischplatte befestigtes Trocknermodul zeigt,
  • Fig. 2 eine gegenüber der Ansicht der Fig. 1 um 90° gedrehte Querschnittsansicht des Trocknermoduls zeigt,
  • Fig. 3 eine Ansicht des erfindungsgemäßen Trocknermoduls von unten ist, und
  • Fig. 4 einen Querschnitt eines verwendeten Filterträgers zeigt.
    • A preferred embodiment of the invention is described below with reference to the drawings, in which
  • 1 shows a schematic cross-sectional view through a dryer module fastened in a table top,
  • FIG. 2 shows a cross-sectional view of the dryer module rotated by 90 ° with respect to the view of FIG. 1,
  • 3 is a bottom view of the dryer module according to the invention, and
  • Fig. 4 shows a cross section of a filter carrier used.

    • In der in der Fig. 1 dargestellten bevorzugten Ausführungsform werden zwei Tangentiallüfter 1 an ihren Längsseiten gekoppelt, um einen möglichst homogenen Luftstrom zu erhalten. Es ergibt sich so ein gleichmäßiger Luftstrom über die gesamte Fläche des zu trocknenden Filterträgers (nicht dargestellt). Die Austrittsfläche des Luftschachts 4, die in der bevorzugten Ausführungsform 8 cm x 12,5 cm beträgt, ist etwas größer als die. zu trocknende Grundfläche des Filterträgers. Der Luftstrom beträgt maximal 2x80 m3/h und kann über einen Vorschalttransformator (nicht dargestellt) über die Umdrehungsgeschwindigkeit der Motore 3 des Paares der Tangentiallüfter 1 geregelt werden.In the preferred embodiment shown in FIG. 1, two tangential fans 1 are coupled on their long sides in order to obtain an air flow that is as homogeneous as possible. This results in a uniform air flow over the entire surface of the filter carrier to be dried (not shown). The outlet area of the air shaft 4, which is 8 cm x 12.5 cm in the preferred embodiment, is slightly larger than that. Base area of the filter carrier to be dried. The air flow is a maximum of 2x80 m 3 / h and can be regulated via a series transformer (not shown) via the rotational speed of the motors 3 of the pair of tangential fans 1.

    Der rechteckige Luftschacht 4 ist so konstruiert, daß der Luftstrom, einer Düse 6 gleich, zur Austrittsöffung hin gebündelt wird. Er ist aus 2 mm starkem Eisenblech gefertigt und zu einer geschlossenen Form verlötet. An dessem unteren Bereich befindet sich eine Aufnahme für eine Heizung 5. Darunter sind die beiden Tangentiallüfter 1 befestigt. Der obere Teil des Luftschachts 4 ist in einer entsprechend dimensionierten Aussparung einer Tischplatte 7 befestigt und somit im Arbeitsbereich an genau definierter Position fixiert. Die Heizelemente 5a, 5b und die Lüfterelemente 1 befinden sich durch diese Anordnung unter dem Arbeitstisch 7. Die Öffnung des Luftschachtes 4 ist vorzugsweise ebenerdig mit der Arbeitsfläche eines Robotertischs (nicht dargestellt) im Falle der Verwendung des Trocknermoduls in einem automatisierten Verfahren.The rectangular duct 4 is constructed so that the Air flow, like a nozzle 6, towards the outlet opening is bundled. It is made of 2 mm thick iron sheet manufactured and soldered to a closed shape. On in the lower area there is a holder for one Heating 5. Below are the two tangential fans 1 attached. The upper part of the air duct 4 is in one correspondingly dimensioned recess in a table top 7 attached and thus in the work area at precisely defined Fixed position. The heating elements 5a, 5b and Fan elements 1 are located under this arrangement the work table 7. The opening of the air shaft 4 is preferably at ground level with the work surface of a Robot tables (not shown) in case of use of the dryer module in an automated process.

    Die Heizung 5 besteht in der bevorzugten Ausführungsform aus zwei Heizelementen 5a, 5b, wie dies in den Figuren 2 und 3 deutlicher werden wird. Diese Heizelemente 5a, 5b sind über der Austrittsöffnung der Tangentiallüfter 1 befestigt. Jeweils zwei Multimicalochplatten werden als Träger für einen Heizdraht verwendet, der in Wendeln von ca. 3 mm Abstand in den Luftstrom eingespannt wird und diesen dadurch gleichmäßig erwärmt. Die Wendel sind dabei so beschaffen, daß die Umkehrschlaufe außerhalb des Luftstroms zu liegen kommt, die parallel verlaufenden Heizdrähte hingegen senkrecht zu den Multimicalochplatten angeordnet sind.The heater 5 is in the preferred embodiment from two heating elements 5a, 5b, as shown in FIGS. 2 and 3 will become clearer. These heating elements 5a, 5b are above the outlet opening of the tangential fan 1 attached. Two Multimicalochplatten are each considered Carrier for a heating wire used in coils of about 3 mm distance is clamped in the air flow and this heats it evenly. The coils are there so that the reverse loop outside the Airflow comes to rest, the parallel Heating wires, on the other hand, are perpendicular to the multi-micro plates are arranged.

    Der Filterständer, bestehend aus einem Aufnahmeelement 11 und Stützelementen 10, ist so ausgelegt, daß er zentrierende Elemente (dargestellt in der Fig. 3) an seinen Kontaktstellen zum Filterträger (nicht dargestellt) aufweist. Insbesondere sind 30° Phasen an den Berührungsbereichen zu den Abstandshülsen (siehe Fig. 4) eines Filterträgers zu nennen. Der Filterständer umfaßt in der bevorzugten Ausführungsform eine als Aufnahmeelement wirkende obere Trägerplatte oder Zentrierplatte 10, welche die Zentrierelemente, nämlich Aussparungen für die Abstandshülsen und die Auflagefläche für den Rand des Filterträgers aufweist. An dieser Zentrierplatte sind in den vier Eckpunkten 4,5 cm hohe Stützelemente 10 befestigt, die an ihrem unteren Ende jeweils einen Befestigungsbolzen 8 von 0,3 cm aufweisen. Diese Bolzen 9 fixieren den Filterträgerständer exakt über den Luftaustrittsschacht 4 des Trocknermoduls, indem sie in entsprechende Bohrungen einer auf dem Arbeitstisch angeordneten Platte 8 zur Befestigung des Ständers eingreifen. In der bevorzugten Ausführungsform ist die Platte 8 aus Acryl. Nach der Einstellung des Filterträgers, der in Fig. 4 beschrieben ist, befinden sich dessen Auslaßdüsen ca 3 cm über der Oberkante des Luftschachts 4.The filter stand, consisting of a mounting element 11 and support members 10 is designed so that it centering elements (shown in Fig. 3) on its Contact points to the filter carrier (not shown) having. In particular, 30 ° phases are on the Contact areas to the spacer sleeves (see Fig. 4) to name a filter carrier. The filter stand includes in the preferred embodiment one as a receiving element acting upper support plate or centering plate 10, which the centering elements, namely cutouts for the Spacer sleeves and the contact surface for the edge of the Has filter carrier. On this centering plate are in attached the four corner points 4.5 cm high support elements 10, which each have a fastening bolt at their lower end 8 of 0.3 cm. These bolts 9 fix the Filter carrier stand exactly over the air outlet shaft 4 the dryer module by inserting it into the appropriate holes a plate 8 arranged on the work table for Intervene to secure the stand. In the preferred Embodiment is the plate 8 made of acrylic. After Setting the filter carrier, which is described in Fig. 4 is, the outlet nozzles are about 3 cm above the Upper edge of the air duct 4.

    Die Ansteuerung der Lüfterelemente 1 sowie die Freischaltung der Heizelemente 5a, 5b erfolgen getrennt über ein Controllerinterface. Somit kann nach Beendigung des Trocknungsvorgangs zuerst die Heizung 5 abgeschaltet werden, so daß nach zeitlichem Versatz die nun kühlend auf die Heizdrähte einwirkende Lüftung beendet wird. Zur Temperaturmessung ist in der Düse 6 eine seitliche Aussparung 13 zur Aufnahme eines Temperaturfühlers vorgesehen. Die Heizelelmente 5a, 5b wird über eine Temperaturschwellwertschaltung gesteuert.The control of the fan elements 1 and the The heating elements 5a, 5b are activated separately via a controller interface. So after finishing of the drying process, the heater 5 is first switched off be, so that after a time delay the now cooling the heating wires acting ventilation is stopped. to Temperature measurement is a lateral one in the nozzle 6 Recess 13 for receiving a temperature sensor intended. The heating elements 5a, 5b is a Temperature threshold switching controlled.

    Fig. 2 zeigt ebenfalls das erfindungsgemäße Trocknermodul in einer Querschnittsansicht, die jedoch gegenüber der Ansicht der Fig. 1 um 90° um die vertikale Symmetrieachse gedreht ist. Zu erkennen sind die beiden parallel angeordneten Tangentiallüfterwalzen 1, die von entsprechenden Motoren 3 angetrieben werden. In dem Luftschacht 4 sind die beiden Heizelemente 5a, 5b der Heizung 5 parallel und fast aneinanderstoßend angeordnet. Darüberliegend ist die Düse 6 als Bestandteil des Schachts 4 zur Bündelung der trockenenden Luft ausgebildet. In der hier dargestellten Ausführungsform ist das Trocknermodul in der Öffnung einer Tischplatte 7 o.ä. befestigt. Auf der Tischplatte 7 ist eine Platte 8, vorzugsweise aus Acryl oder einem ähnlichen Werkstoff, angeordnet, die den Filterträgerständer bestehend aus den Stützelementen 9 und der Zentrierplatte 11 trägt, wobei der Filterträgerständer mittels Befestigungsbolzen 9 in der Platte 8 fixiert und ausgerichtet ist.2 also shows the dryer module according to the invention in a cross-sectional view, but opposite the 1 of 90 ° around the vertical axis of symmetry is rotated. The two can be seen in parallel arranged tangential fan rollers 1 by corresponding motors 3 are driven. By doing Air duct 4 are the two heating elements 5a, 5b Heater 5 arranged in parallel and almost abutting. Above this, the nozzle 6 is part of the shaft 4 designed to bundle the drying air. In the The embodiment shown here is the dryer module in the opening of a table top 7 or the like attached. On the Table top 7 is a top 8, preferably made of acrylic or a similar material, arranged the Filter support stand consisting of the support elements 9 and the centering plate 11 carries, the filter support stand fixed by means of fastening bolts 9 in the plate 8 and is aligned.

    Fig. 3 zeigt das oben beschrieben Trocknermodul in einer Ansicht von oben. Es ist dabei zu berücksichtigen, daß in der bevorzugten Ausführungsform die Platte 8 aus Acryl besteht und damit durchsichtig ist. Dargestellt sind zwei Tangentiallüfter 1 mit Antriebsverbindungen 2 und Motoren 3, die Heizelelemente 5a und 5b im Luftschacht 4, der eine Düse 6 bildet. Die Zentrierplatte 11 weist vier Zentrierschächt 12 zur Aufnahme entsprechend geformter Abstandshülsen des Filterträgers auf, die in der Fig. 4 dargestellt sind . Durch diese Zentrierschächte 12 wird eine Ausrichtung des Filterträgers erzwungen.3 shows the dryer module described above in one View from above. It should be noted that in the preferred embodiment, the plate 8 made of acrylic exists and is therefore transparent. Two are shown Tangential fan 1 with drive connections 2 and motors 3, the heating elements 5a and 5b in the air shaft 4, the one Nozzle 6 forms. The centering plate 11 has four Centering shaft 12 for receiving appropriately shaped Spacer sleeves of the filter carrier, which in FIG. 4 are shown. Through these centering shafts 12 alignment of the filter carrier is forced.

    Fig. 4 zeigt zur Erläuterung einen Querschnitt durch einen üblicherweise verwendeten Filterträger 14, der aus einer Vielzahl von Filterelementen 15 gebildet wird, die auf bekannte Weise miteinander verbunden sind. Üblicherweise hat der Filterträger in Draufsicht eine rechteckige Gestalt. Die äußeren Filterelemente 15 des Filterträger 14 weisen Abstandshülsen 17 auf, die zur Zentrierung des Filterträgers 14 in einer automatisierten Umgebung dienen. Hier dienen sie zur Zentrierung in den Zentrierschächten 12 der Zentrierplatte 11. Ferner weist der Filterträger 14 eine als Kragen ausgeformte umlaufende Wandung 18 auf. Beim Einführen des Filterträgers in die Zentrierplatte 11 des Trocknermoduls greifen zuerst die Abstandshülsen 17 in die Zentrierschächte 12 ein und der Filterträger 14 wird beispielsweise durch einen Roboter solange nach unten bewegt, bis die Wandung 18 auf die Zentrierplatte 11 aufsetzt und der Filterträger 14 so seine Endposition einnimmt. Danach wird das Trocknermodul in Betrieb genommen. Fig. 4 shows a cross section through for explanation a commonly used filter carrier 14, the a plurality of filter elements 15 is formed, the are connected to each other in a known manner. The filter carrier usually has a top view rectangular shape. The outer filter elements 15 of the Filter carrier 14 have spacer sleeves 17 which for Centering of the filter carrier 14 in an automated Serve environment. Here they are used for centering in the Centering shafts 12 of the centering plate 11. Further points the filter carrier 14 has a circumferential shape formed as a collar Wall 18 on. When inserting the filter carrier into the Centering plate 11 of the dryer module first grip the Spacer sleeves 17 in the centering shafts 12 and Filter carrier 14 is, for example, by a robot moved down until the wall 18 on the Centering plate 11 and the filter carrier 14 so its Takes the final position. Then the dryer module is in Put into operation.

    BezugszeichenlisteLIST OF REFERENCE NUMBERS

    11
    TangentiallüfterwalzeTangentiallüfterwalze
    22
    Antriebsverbindungdrive connection
    33
    Motorengine
    44
    Luftschachtairshaft
    55
    Heizungheater
    5a, 5b5a, 5b
    Heizelementheating element
    66
    Düsejet
    77
    Tischplattetabletop
    88th
    Acrylplatteacrylic plate
    99
    Befestigungsbolzenmounting bolts
    1010
    Ständerstand
    1111
    Zentrierplattecentering
    1212
    ZentrierschachtZentrierschacht
    1313
    TemperaturfühleraufnahmeTemperature sensor connection
    1414
    Filterträgerfilter support
    1515
    Filterelementfilter element
    1616
    FilterelementdüseFilterelementdüse
    1717
    Abstandshülsespacer
    1818
    Wandungwall

    Claims (10)

    1. Dryer module for drying a filter support (14) in the context of DNA preparation, having a heating system (5) and a blower member (11), the heating system (5) being arranged downstream of the blower member (1) and the filter (15) arranged in the filter support (14) being blown on and dried from below,
      characterized in that
      the filters (15) are arranged suspended in the filter support (14) and the filter support has spacer sleeves (17) that are insertable into corresponding centring shafts (12) in a centring plate (11) for receiving the filter support (14), the heating system (5) comprising heating wires, which extend in parallel over a rectangular cross-section of an air shaft (4) and which are arranged at right angles to the air flow and upstream of the centring plate (11), and the dryer module having a crossflow blower as blower member (1).
    2. Dryer module according to claim 1, characterized in that the blower member (1) is driven by an electric motor (3).
    3. Dryer module according to claim 1 or 2, characterized in that a nozzle (6) for channeling the air flow is arranged between the air shaft (4) and the centring member (11) for the filter support (14).
    4. Dryer module according to any one of the preceding claims, characterized in that the dryer module is arranged in an opening in a bench top (7).
    5. Dryer module according to any one of the preceding claims 1 to 4, characterized in that the centring plate (11) for receiving the filter support is arranged on supporting members (10).
    6. Dryer module according to any one of the preceding claims, characterized in that the module has a temperature sensor.
    7. Dryer module according to claim 6, characterized in that the module has an electrical control means for controlling the motor (3), of which there is at least one, and the heating system (5), the heating system being controlled as a function of the temperature measured by the temperature sensor.
    8. Dryer module according to claim 7, characterized in that the control means is provided with a delay circuit which, when the dryer module is switched off, causes the blower member (1), of which there is at least one, to be switched off chronologically after the heating system (5) is switched off.
    9. Use of the dryer module according to any one of claims I to 8 in an automated environment.
    10. Use of the dryer module according to any one of claims 1 to 8 as a stand-alone apparatus.
    EP98940128A 1997-07-03 1998-07-03 Drying module Expired - Lifetime EP0993586B1 (en)

    Applications Claiming Priority (3)

    Application Number Priority Date Filing Date Title
    DE19728496 1997-07-03
    DE19728496A DE19728496A1 (en) 1997-07-03 1997-07-03 Dryer module
    PCT/EP1998/004121 WO1999001708A1 (en) 1997-07-03 1998-07-03 Drying module

    Publications (2)

    Publication Number Publication Date
    EP0993586A1 EP0993586A1 (en) 2000-04-19
    EP0993586B1 true EP0993586B1 (en) 2002-01-23

    Family

    ID=7834575

    Family Applications (1)

    Application Number Title Priority Date Filing Date
    EP98940128A Expired - Lifetime EP0993586B1 (en) 1997-07-03 1998-07-03 Drying module

    Country Status (11)

    Country Link
    US (1) US6226892B1 (en)
    EP (1) EP0993586B1 (en)
    JP (1) JP2002510383A (en)
    AT (1) ATE212433T1 (en)
    AU (1) AU8855398A (en)
    CA (1) CA2295145A1 (en)
    DE (2) DE19728496A1 (en)
    DK (1) DK0993586T3 (en)
    ES (1) ES2172183T3 (en)
    PT (1) PT993586E (en)
    WO (1) WO1999001708A1 (en)

    Families Citing this family (2)

    * Cited by examiner, † Cited by third party
    Publication number Priority date Publication date Assignee Title
    KR100487345B1 (en) * 2002-11-26 2005-05-03 엘지전자 주식회사 Dryer and Control Method of Cooling Time for the same
    CN106369967A (en) * 2016-10-31 2017-02-01 王月娟 Agricultural fodder dryer

    Family Cites Families (15)

    * Cited by examiner, † Cited by third party
    Publication number Priority date Publication date Assignee Title
    DE97363C (en) *
    FR880989A (en) * 1941-12-08 1943-04-12 Fraissec Vegetable and fruit dehydrating device
    US3007256A (en) * 1957-09-12 1961-11-07 Auguste L M A Rouy Heating and drying apparatus and method
    US4812621A (en) * 1987-10-28 1989-03-14 Tania Brotherton Electrically heated dish drying rack
    US5216251A (en) * 1991-10-18 1993-06-01 Matschke Arthur L Apparatus and method for a bio-conditioning germicidal dryer
    EP0569115A3 (en) * 1992-05-05 1994-01-05 General Atomics High throughput DNA preparation system
    US6068874A (en) * 1993-02-16 2000-05-30 Dehydration Technologies, Inc. Process of dehydrating biological products
    US5311671A (en) * 1993-03-23 1994-05-17 Brooks Floyd L Apparatus for recovering dry cleaning fluid
    JPH0712458A (en) * 1993-06-23 1995-01-17 Murata Mfg Co Ltd Dryer for parts
    US5440822A (en) * 1993-07-16 1995-08-15 Novel Experimental Technology Method and apparatus for drying electrophoresis gels
    US5709038A (en) * 1993-09-24 1998-01-20 Optimum Air Corporation Automated air filtration and drying system for waterborne paint and industrial coatings
    DE9401195U1 (en) * 1994-01-25 1994-03-31 Bartel, Walter, 78054 Villingen-Schwenningen Sieve drying cabinet
    US5451524A (en) * 1994-02-01 1995-09-19 The Gillette Company In vitro chamber for human organ tissue samples
    DE9413225U1 (en) * 1994-08-16 1995-03-16 Wiesenborn, Wilfried, 41812 Erkelenz Drying unit
    US5704135A (en) * 1995-07-10 1998-01-06 Riahi; Sohrab Instant photographic print drying device

    Also Published As

    Publication number Publication date
    US6226892B1 (en) 2001-05-08
    ES2172183T3 (en) 2002-09-16
    PT993586E (en) 2002-07-31
    DK0993586T3 (en) 2002-04-22
    WO1999001708A1 (en) 1999-01-14
    JP2002510383A (en) 2002-04-02
    EP0993586A1 (en) 2000-04-19
    AU8855398A (en) 1999-01-25
    DE19728496A1 (en) 1999-01-07
    DE59802912D1 (en) 2002-03-14
    CA2295145A1 (en) 1999-01-14
    ATE212433T1 (en) 2002-02-15

    Similar Documents

    Publication Publication Date Title
    EP1278448B1 (en) Device for carrying out works on a surface
    EP1053581B1 (en) Control cabinet with cooling device
    EP2030689B1 (en) Microplate support with magnets
    WO1993022650A2 (en) Device for evaporating preferably liquid substances, in particular reagents, or for preparing or analyzing sampled materials
    EP0813230B1 (en) Method for dry cleaning dust soiled objects for carrying and keeping semi-conductor wafers
    DE3623511A1 (en) METHOD AND DEVICE FOR DRYING CERAMIC HOLLOW BODIES
    EP1427569A1 (en) Hand machine-tool comprising a dust extraction device
    DE102007047772A1 (en) Temperature chamber for tempering electronic components, in particular IC's
    DE102008012333B4 (en) Device for the thermal treatment of disc-shaped substrates
    EP0993586B1 (en) Drying module
    WO2019081271A1 (en) Combination appliance with fume extraction device and cooktop
    DE1752727B2 (en) Method and device for dismantling a semiconductor wafer
    DE69727322T2 (en) METHOD AND DEVICE FOR REDUCING NOISE FROM A FILTER FAN UNIT
    EP2431681A1 (en) Heating, method for heating and laminating and spray drier
    EP0356611B1 (en) Radiation apparatus
    DE60121651T2 (en) Evaporator with hot air bath
    EP1368124A1 (en) Accommodating device for specimen slides
    DE3102029A1 (en) Appliance for separating ferromagnetic particles
    EP0593143A2 (en) Method for the recycling of television tubes
    DE2839592C2 (en)
    DE3714970A1 (en) METHOD AND DEVICE FOR CERAMIZING GLASS PANELS
    DE202019105486U1 (en) Device for drying laundry
    DE19516713C1 (en) Device for sepg. of culture samples from sheet base culture dishes
    EP1712283A1 (en) Device, apparatus comprising said device and a method for drying wells and filters of micro-titer filter plates
    DE3249664C2 (en) Air-swirl column for removing air, gas and dust in pollutant-dispersal systems

    Legal Events

    Date Code Title Description
    PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

    Free format text: ORIGINAL CODE: 0009012

    17P Request for examination filed

    Effective date: 20000105

    AK Designated contracting states

    Kind code of ref document: A1

    Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

    17Q First examination report despatched

    Effective date: 20001030

    GRAG Despatch of communication of intention to grant

    Free format text: ORIGINAL CODE: EPIDOS AGRA

    GRAG Despatch of communication of intention to grant

    Free format text: ORIGINAL CODE: EPIDOS AGRA

    GRAH Despatch of communication of intention to grant a patent

    Free format text: ORIGINAL CODE: EPIDOS IGRA

    GRAH Despatch of communication of intention to grant a patent

    Free format text: ORIGINAL CODE: EPIDOS IGRA

    GRAA (expected) grant

    Free format text: ORIGINAL CODE: 0009210

    REG Reference to a national code

    Ref country code: GB

    Ref legal event code: IF02

    AK Designated contracting states

    Kind code of ref document: B1

    Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

    REF Corresponds to:

    Ref document number: 212433

    Country of ref document: AT

    Date of ref document: 20020215

    Kind code of ref document: T

    REG Reference to a national code

    Ref country code: CH

    Ref legal event code: NV

    Representative=s name: E. BLUM & CO. PATENTANWAELTE

    Ref country code: CH

    Ref legal event code: EP

    REG Reference to a national code

    Ref country code: IE

    Ref legal event code: FG4D

    Free format text: GERMAN

    REF Corresponds to:

    Ref document number: 59802912

    Country of ref document: DE

    Date of ref document: 20020314

    REG Reference to a national code

    Ref country code: DK

    Ref legal event code: T3

    GBT Gb: translation of ep patent filed (gb section 77(6)(a)/1977)

    Effective date: 20020407

    PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

    Ref country code: PT

    Payment date: 20020531

    Year of fee payment: 5

    PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

    Ref country code: GB

    Payment date: 20020610

    Year of fee payment: 5

    PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

    Ref country code: GR

    Payment date: 20020626

    Year of fee payment: 5

    PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

    Ref country code: IE

    Payment date: 20020627

    Year of fee payment: 5

    PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

    Ref country code: DE

    Payment date: 20020713

    Year of fee payment: 5

    PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

    Ref country code: NL

    Payment date: 20020717

    Year of fee payment: 5

    Ref country code: FR

    Payment date: 20020717

    Year of fee payment: 5

    PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

    Ref country code: DK

    Payment date: 20020722

    Year of fee payment: 5

    Ref country code: MC

    Payment date: 20020722

    Year of fee payment: 5

    Ref country code: AT

    Payment date: 20020722

    Year of fee payment: 5

    PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

    Ref country code: SE

    Payment date: 20020723

    Year of fee payment: 5

    Ref country code: BE

    Payment date: 20020723

    Year of fee payment: 5

    Ref country code: LU

    Payment date: 20020723

    Year of fee payment: 5

    Ref country code: FI

    Payment date: 20020723

    Year of fee payment: 5

    PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

    Ref country code: ES

    Payment date: 20020724

    Year of fee payment: 5

    PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

    Ref country code: CH

    Payment date: 20020726

    Year of fee payment: 5

    PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

    Ref country code: CY

    Payment date: 20020731

    Year of fee payment: 5

    REG Reference to a national code

    Ref country code: PT

    Ref legal event code: SC4A

    Free format text: AVAILABILITY OF NATIONAL TRANSLATION

    Effective date: 20020416

    REG Reference to a national code

    Ref country code: GR

    Ref legal event code: EP

    Ref document number: 20020401405

    Country of ref document: GR

    REG Reference to a national code

    Ref country code: ES

    Ref legal event code: FG2A

    Ref document number: 2172183

    Country of ref document: ES

    Kind code of ref document: T3

    PLBE No opposition filed within time limit

    Free format text: ORIGINAL CODE: 0009261

    STAA Information on the status of an ep patent application or granted ep patent

    Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

    26N No opposition filed
    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: LU

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20030703

    Ref country code: IE

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20030703

    Ref country code: GB

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20030703

    Ref country code: FI

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20030703

    Ref country code: AT

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20030703

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: SE

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20030704

    Ref country code: ES

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20030704

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: MC

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20030731

    Ref country code: LI

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20030731

    Ref country code: DK

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20030731

    Ref country code: CY

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20030731

    Ref country code: CH

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20030731

    Ref country code: BE

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20030731

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: DE

    Free format text: LAPSE BECAUSE OF THE APPLICANT RENOUNCES

    Effective date: 20040115

    BERE Be: lapsed

    Owner name: G.- FUR BIOTECHNOLOGISCHE FORSCHUNG M.B.H. *GBF

    Effective date: 20030731

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: PT

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20040131

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: NL

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20040201

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: GR

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20040205

    GBPC Gb: european patent ceased through non-payment of renewal fee

    Effective date: 20030703

    EUG Se: european patent has lapsed
    REG Reference to a national code

    Ref country code: CH

    Ref legal event code: PL

    REG Reference to a national code

    Ref country code: DK

    Ref legal event code: EBP

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: FR

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20040331

    NLV4 Nl: lapsed or anulled due to non-payment of the annual fee

    Effective date: 20040201

    REG Reference to a national code

    Ref country code: FR

    Ref legal event code: ST

    Ref country code: PT

    Ref legal event code: MM4A

    Free format text: LAPSE DUE TO NON-PAYMENT OF FEES

    Effective date: 20040131

    REG Reference to a national code

    Ref country code: IE

    Ref legal event code: MM4A

    REG Reference to a national code

    Ref country code: ES

    Ref legal event code: FD2A

    Effective date: 20030704

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: IT

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES;WARNING: LAPSES OF ITALIAN PATENTS WITH EFFECTIVE DATE BEFORE 2007 MAY HAVE OCCURRED AT ANY TIME BEFORE 2007. THE CORRECT EFFECTIVE DATE MAY BE DIFFERENT FROM THE ONE RECORDED.

    Effective date: 20050703