EP0993586A1 - Drying module - Google Patents

Drying module

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Publication number
EP0993586A1
EP0993586A1 EP98940128A EP98940128A EP0993586A1 EP 0993586 A1 EP0993586 A1 EP 0993586A1 EP 98940128 A EP98940128 A EP 98940128A EP 98940128 A EP98940128 A EP 98940128A EP 0993586 A1 EP0993586 A1 EP 0993586A1
Authority
EP
European Patent Office
Prior art keywords
dryer module
module according
filter carrier
filter
dryer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP98940128A
Other languages
German (de)
French (fr)
Other versions
EP0993586B1 (en
Inventor
Helmut BLÖCKER
Gerhard Kauer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Original Assignee
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helmholtz Zentrum fuer Infektionsforschung HZI GmbH filed Critical Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Publication of EP0993586A1 publication Critical patent/EP0993586A1/en
Application granted granted Critical
Publication of EP0993586B1 publication Critical patent/EP0993586B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26—DRYING
    • F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B9/00—Machines or apparatus for drying solid materials or objects at rest or with only local agitation; Domestic airing cupboards
    • F26B9/003—Small self-contained devices, e.g. portable
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26—DRYING
    • F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B21/00—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26—DRYING
    • F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B9/00—Machines or apparatus for drying solid materials or objects at rest or with only local agitation; Domestic airing cupboards
    • F26B9/06—Machines or apparatus for drying solid materials or objects at rest or with only local agitation; Domestic airing cupboards in stationary drums or chambers

Definitions

  • the invention relates to a dryer module for drying filter carriers, such as those used in DNA preparation.
  • the preparation protocol described by Qiagen includes washing the DNA adsorbed in filter elements of a filter support with ethanol-containing buffer. Experiments showed that traces of ethanol drastically deteriorate the result of the subsequent sequencing reactions. The enzymes involved are likely to be inhibited by ethanol. In this step, the larger amounts of ethanol are first removed from the filters
  • Implementation of the protocol for example in an appropriate vacuum apparatus, can be achieved by applying the applied vacuum longer than would be necessary to remove the washing liquid.
  • the air flow generated in this way in the filter removes the larger amounts of ethanol from the filters.
  • the special design of the Qiagen filter carrier contaminates the subsequent sequencing process, unless special precautions are taken, since ethanol residues on the walls between the outlet nozzles and the
  • the invention is therefore based on the object of providing a device with which the remaining residual alcohol is removed from filter carriers, as are used, for example, in the Qiagen protocol.
  • the invention relates to a dryer module for drying filter carriers in the DNA preparation, the dryer module being a receiving element for receiving the Has filter carrier and at least one fan element, wherein the fan element is arranged below the receiving element for receiving the filter carrier, so that the filter carrier is blown from below and dried.
  • the dryer module according to the invention preferably has at least one heating element for heating the air blown against the filter carrier from below.
  • an air shaft for guiding the dry air is arranged between the fan and the receptacle of the filter carrier, in which the heating elements are arranged.
  • the fan element is advantageously formed by a tangential fan which is driven by an electric motor.
  • the air shaft can be designed at its upper end as a nozzle for bundling the lateral air flow.
  • the receiving element of the filter carrier is advantageously designed as a centering plate, the centering plate being arranged on base elements, so that the centering plate forms a filter carrier stand together with the base elements.
  • the dryer module can be arranged in an opening of a table top.
  • the dryer module can also be operated as a stand-alone device, both in an automated environment and as a stand-alone device.
  • the step of removing the ethanol from the area between the outlet nozzle and the plastic casing of the filter carrier is not manual performed, but automated.
  • the dryer module blows heated air (maximum 50 ° C) from below against the filter holder and the ethanol evaporates.
  • the filter dried in this way is placed again in a vacuum chamber, in order to then be ventilated again for a certain time by an applied vacuum.
  • the remaining traces of ethanol are removed.
  • the filter treated in this way is again placed on the dryer module in order to ensure, by means of the temperature-controlled air flow, that any traces of ethanol that may still be present are finally evaporated. Only then is the filter carrier put back into the pipetting robot, for example, in order to pipette in the eluent for releasing the DNA from the filters.
  • FIG. 1 shows a schematic cross-sectional view through a dryer module fastened in a table top
  • FIG. 2 shows a cross-sectional view of the dryer module rotated by 90 ° with respect to the view of FIG. 1,
  • FIG. 3 is a bottom view of the dryer module according to the invention.
  • Fig. 4 shows a cross section of a filter carrier used.
  • two tangential fans 1 are coupled on their long sides in order to obtain an air flow that is as homogeneous as possible. This results in an even air flow over the entire surface of the material to be dried Filter carrier (not shown).
  • the outlet area of the air shaft 4, which is 8 cm x 12.5 cm in the preferred embodiment, is somewhat larger than the base area of the filter carrier to be dried.
  • the air flow is a maximum of 2x80 m 3 / h and can be regulated via a series transformer (not shown) via the rotational speed of the motors 3 of the pair of tangential fans 1.
  • the rectangular air duct 4 is constructed in such a way that the air flow, like a nozzle 6, is bundled towards the outlet opening. It is made of 2 mm thick iron sheet and soldered to a closed shape. At its lower area there is a receptacle for a heater 5. The two tangential fans 1 are fastened underneath. The upper part of the air duct 4 is fastened in a correspondingly dimensioned recess in a table top 7 and is thus fixed in the work area at a precisely defined position. As a result of this arrangement, the heating elements 5a, 5b and the fan elements 1 are located under the work table 7.
  • the opening of the air shaft 4 is preferably at ground level with the work surface of a robot table (not shown) in the case of using the dryer module in an automated process.
  • the heater 5 consists of two heating elements 5a, 5b, as will become clearer in FIGS. 2 and 3. These heating elements 5a, 5b are fastened over the outlet opening of the tangential fan 1.
  • Two multimicaloch plates each are used as a support for a heating wire, which is clamped in coils at a distance of approx. 3 mm in the air flow and thereby heats it evenly.
  • the helixes are designed so that the reverse loop comes to lie outside the air flow, the parallel ones Heating wires, however, are arranged perpendicular to the multi icalochplatten.
  • the filter stand consisting of a receiving element 11 and support elements 10, is designed such that it has centering elements (shown in FIG. 3) at its contact points with the filter carrier (not shown). In particular, 30 ° phases at the contact areas to the spacer sleeves (see FIG. 4) of a filter carrier should be mentioned.
  • the filter stand comprises an upper carrier plate or centering plate 10, which acts as a receiving element and which has the centering elements, namely recesses for the spacer sleeves and the contact surface for the edge of the filter carrier. On this centering plate, 4.5 cm high supporting elements 10 are fastened in the four corner points, each of which has a fastening bolt 8 of 0.3 cm at its lower end.
  • bolts 9 fix the filter support stand exactly over the air outlet shaft 4 of the dryer module by engaging in corresponding bores in a plate 8 arranged on the work table for fastening the stand.
  • plate 8 is made of acrylic.
  • the control of the fan elements 1 and the activation of the heating elements 5a, 5b take place separately via a controller interface.
  • the heater 5 can first be switched off, so that the ventilation, which is now cooling on the heating wires, is ended after a time delay.
  • the heating elements 5a, 5b are controlled via a temperature threshold circuit.
  • FIG. 2 also shows the dryer module according to the invention in a cross-sectional view, which, however, is rotated by 90 ° around the vertical axis of symmetry compared to the view in FIG. 1.
  • the two heating elements 5a, 5b of the heater 5 are arranged in parallel and almost abutting one another.
  • the nozzle 6 is designed as part of the shaft 4 for bundling the drying air.
  • the dryer module is in the opening of a table top 7 or the like. attached.
  • a plate 8 preferably made of acrylic or a similar material, is arranged, which supports the filter support stand consisting of the support elements 9 and the centering plate 11, the filter support stand being fixed and aligned in the plate 8 by means of fastening bolts 9.
  • the plate 8 is made of acrylic and is therefore transparent. Shown are two tangential fans 1 with drive connections 2 and motors 3, the heating elements 5a and 5b in the air shaft 4, which forms a nozzle 6.
  • the centering plate 11 has four centering chutes 12 for receiving correspondingly shaped spacer sleeves of the filter carrier, which are shown in FIG. 4. An alignment of the filter carrier is forced through these centering shafts 12.
  • FIG. 4 shows a cross section through a commonly used filter carrier 14 for explanation o a plurality of filter elements 15 is formed, which are connected to one another in a known manner.
  • the filter carrier usually has a rectangular shape in plan view.
  • the outer filter elements 15 of the filter carrier 14 have spacer sleeves 17 which serve to center the filter carrier 14 in an automated environment. Here they serve for centering in the centering shafts 12 of the centering plate 11.
  • the filter carrier 14 has a circumferential wall 18 shaped as a collar.
  • the spacer sleeves 17 When the filter carrier is inserted into the centering plate 11 of the dryer module, the spacer sleeves 17 first engage in the centering shafts 12 and the filter carrier 14 is moved downward, for example by a robot, until the wall 18 touches the centering plate 11 and the filter carrier 14 thus reaches its end position occupies. Then the dryer module is put into operation.

Landscapes

  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Drying Of Solid Materials (AREA)
  • Inorganic Insulating Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A dryer module for drying filter supports in DNA preparation comprises a receiving member for receiving the filter support and at least one blower member, the blower member being arranged below the receiving member for receiving the filter support so that the filter support is blown on an dried from below. The dryer module also has a heating system for warming the air blown from below against the filter support, the heating system being arranged above the blower member, of which there is at least one. The dryer module is electronically controlled by a control means as a function of the measured temperature of the drying air. The dryer module can be used as a stand-along apparatus or in an automated environment.

Description

Trocknermodul Dryer module
Die Erfindung betrifft ein Trocknermodul zur Trocknung von Filterträgern, wie sie bei der DNA-Präparation verwendet werden.The invention relates to a dryer module for drying filter carriers, such as those used in DNA preparation.
Bei modernen DNA-Präparationen, deren Produkte zu Sequenzieraufgaben eingesetzt werden sollen, ist eine Automatisierung der arbeitsintensiven manuellen Schritte unabdingbar, was insbesondere bei den neueren Megasequenzierproj ekten deutlich wird (Human Genome Project) . Während früher keine sogenannten Fertig-Kits zur Verfügung standen, welche die Aufarbeitung einklonierter DNA-Sequenzen vereinfachten, existieren heute bereits einige kommerziell verfügbare Hilfsmittel, die sich auch über Roboter zur automatischen Abarbeitung der entsprechenden Protokolle eignen (wie beispielsweise dem weitverbreiteten Qiagen-Protokoll) .With modern DNA preparations, the products of which are to be used for sequencing tasks, automation of the labor-intensive manual steps is essential, which is particularly evident in the newer megasequencing projects (Human Genome Project). While there were no so-called ready-made kits available to simplify the processing of cloned DNA sequences, there are already a number of commercially available tools that can also be used by robots to automatically process the relevant protocols (such as the widely used Qiagen protocol).
Das von Qiagen beschriebene Präparationsprotokoll beinhaltet das Waschen der in Filterelementen eines Filterträgers adsorbierten DNA mit ethanolhaltigem Puffer. Versuche zeigten, daß Spuren von Ethanol das Ergebnis der nachfolgenden Sequenzierreaktionen drastisch verschlechtern. Die beteiligten Enzyme werden wahrscheinlich von Ethanol inhibiert. In diesem Schritt werden zunächst die größeren Mengen Ethanol aus den FilternThe preparation protocol described by Qiagen includes washing the DNA adsorbed in filter elements of a filter support with ethanol-containing buffer. Experiments showed that traces of ethanol drastically deteriorate the result of the subsequent sequencing reactions. The enzymes involved are likely to be inhibited by ethanol. In this step, the larger amounts of ethanol are first removed from the filters
BESTATIGUNGSKOPIE auf bekannte Weise entfernt. Dies kann bei einerCONFIRMATION COPY removed in a known manner. This can happen with a
Durchführung des Protokolls beispielsweise in einer entsprechenden Vakuumapparatur dadurch bewerkstelligt werden, daß das anliegende Vakuum länger appliziert wird, als dies zur Beseitung der Waschflüssigkeit notwendig wäre. Der auf diese Weise im Filter erzeugte Luftstrom entfernt die größeren Mengen Ethanol aus den Filtern. Die besondere Konstruktion der Qiagenfilterträger verunreinigt jedoch den nachfolgenden Sequenzierprozeß, wenn nicht besondere Vorkehrungen getroffen werden, da Ethanolreste an den Wandungen zwischen den Auslaßdüsen und denImplementation of the protocol, for example in an appropriate vacuum apparatus, can be achieved by applying the applied vacuum longer than would be necessary to remove the washing liquid. The air flow generated in this way in the filter removes the larger amounts of ethanol from the filters. The special design of the Qiagen filter carrier, however, contaminates the subsequent sequencing process, unless special precautions are taken, since ethanol residues on the walls between the outlet nozzles and the
Kunststoffummantelungen an der Unterseite des Filterträgers verbleiben. Es konnte weiter beobachtet werden, daß eine Verunreinigung durch Ethanol während der Elution der DNA aus dem Filtermaterial in folgender Weise stattfindet: das Eluat bildet während des Absaugens in den Eluatfängern einen Tropfen an der Auslaßdüse. Dieser Tropfen "wandert" vor seinem Abreißen an den Düsen hoch und berührt die sich dort noch befindliche ethanolhaltige Flüssigkeit. Es findet folglich eine Durchmischung statt. Der Tropfen reißt dann ab und fällt in den Eluatfänger. Somit ist eine generelle Verunreinigung des Eluats mit Ethanol gegeben, die so groß ist, daß eine "Vergiftung" des Sequenzieransatzes vorliegt.Plastic sheaths remain on the underside of the filter holder. It could also be observed that contamination by ethanol during the elution of the DNA from the filter material takes place in the following way: the eluate forms a drop in the eluate catchers during the aspiration. Before being torn off, this drop "wanders" up the nozzles and touches the ethanol-containing liquid that is still there. Mixing therefore takes place. The drop then tears off and falls into the eluate. This results in a general contamination of the eluate with ethanol which is so large that the sequencing mixture is "poisoned".
Der Erfindung liegt daher die Aufgabe zugrunde, eine Vorrichtung zu schaffen, mit der der verbleibende Restalkohol von Filterträgern entfernt wird, wie sie beispielsweise in dem Qiagenprotokoll verwendet werden.The invention is therefore based on the object of providing a device with which the remaining residual alcohol is removed from filter carriers, as are used, for example, in the Qiagen protocol.
Die Aufgabe wird durch die Merkmale des Anspruchs 1 gelöst. Vorteilhafte Ausgestaltungen der Erfindung sind Gegenstand der Unteransprüche.The object is solved by the features of claim 1. Advantageous embodiments of the invention are the subject of the dependent claims.
Die Erfindung betrifft ein Trocknermodul zum Trocknen von Filterträgern bei der DNA-Präparation, wobei das Trocknermodul ein Aufnahmeelement zur Aufnahme des Filterträgers und mindestens ein Lüfterelement aufweist, wobei das Lüfterelement unterhalb des Aufnahmeelements zur Aufnahme des Filterträgers angeordnet ist, so daß der Filterträger von unten angeblasen und getrocknet wird.The invention relates to a dryer module for drying filter carriers in the DNA preparation, the dryer module being a receiving element for receiving the Has filter carrier and at least one fan element, wherein the fan element is arranged below the receiving element for receiving the filter carrier, so that the filter carrier is blown from below and dried.
Vorzugsweise weist das erfindungsgemäße Trocknermodul mindestens ein Heizelement zum Erwärmen der von unten gegen den Filterträger geblasenen Luft auf.The dryer module according to the invention preferably has at least one heating element for heating the air blown against the filter carrier from below.
Ferner ist zwischen dem Lüfter und der Aufnahme des Filterträgers ein Luftschacht zum Führen der Trockenluft angeordnet, in dem die Heizelemente angeordnet sind.Furthermore, an air shaft for guiding the dry air is arranged between the fan and the receptacle of the filter carrier, in which the heating elements are arranged.
Vorteilhafterweise wird das Lüfterelement durch einen Tangentiallüfter gebildet, der durch einen elektrischen Motor angetrieben wird.The fan element is advantageously formed by a tangential fan which is driven by an electric motor.
Ferner kann der Luftschacht an seinem oberen Ende als Düse zur Bündelung des seitlichen Luftstromes ausgebildet sein.Furthermore, the air shaft can be designed at its upper end as a nozzle for bundling the lateral air flow.
Vorteilhafterweise ist das Aufnahmeelement des Filterträgers als Zentrierplatte ausgebildet, wobei die Zentrierplatte auf Fußelementen angeordnet ist, so daß die Zentrierplatte zusammen mit den Fußelementen einen Filterträgerständer bilden.The receiving element of the filter carrier is advantageously designed as a centering plate, the centering plate being arranged on base elements, so that the centering plate forms a filter carrier stand together with the base elements.
Ferner kann das Trocknermodul in einer Öffnung einer Tischplatte angeordnet sein. Das Trocknermodul kann jedoch auch als ein eigenständiges Gerät betrieben werden, und zwar sowohl in einer automatisierten Umgebung als auch als stand-alone Gerät.Furthermore, the dryer module can be arranged in an opening of a table top. However, the dryer module can also be operated as a stand-alone device, both in an automated environment and as a stand-alone device.
Vorteilhafterweise wird der Schritt des Entfernens des Ethanols aus dem Bereich zwischen Auslaßdüse und Kunststoffummantelung der Filterträger nicht manuell durchgeführt, sondern automatisiert. Zu diesem Zwecke bläst das Trocknermodul erwärmte Luft (maximal 50 °C) von unten gegen den Filterträger und das Ethanol verdampft. Der auf diese Weise getrocknete Filter wird erneut in eine Vakuumkammer gestellt, um dann nochmals für eine gewisse Zeit durch ein angelegtes Vakuum belüftet zu werden. In diesem zweiten Schritt werden verbliebene Ethanolspuren weiter ausgetragen. Abschließend wird das so behandelte Filter nochmals auf das Trocknermodul gebracht, um durch den temperierten Luftstrom sicherzustellen, daß eventuell immer noch vorliegende Spuren von Ethanol endgültig abgedampft werden. Erst dann wird der Filterträger beispielsweise wieder in den Pipettierroboter eingestellt, um das Elutionsmittel für die Freisetzung der DNA aus den Filtern einzupipettieren .Advantageously, the step of removing the ethanol from the area between the outlet nozzle and the plastic casing of the filter carrier is not manual performed, but automated. For this purpose, the dryer module blows heated air (maximum 50 ° C) from below against the filter holder and the ethanol evaporates. The filter dried in this way is placed again in a vacuum chamber, in order to then be ventilated again for a certain time by an applied vacuum. In this second step, the remaining traces of ethanol are removed. Finally, the filter treated in this way is again placed on the dryer module in order to ensure, by means of the temperature-controlled air flow, that any traces of ethanol that may still be present are finally evaporated. Only then is the filter carrier put back into the pipetting robot, for example, in order to pipette in the eluent for releasing the DNA from the filters.
Eine bevorzugte Ausführungsform der Erfindung wird nachfolgend anhand der Zeichnungen beschrieben, in denenA preferred embodiment of the invention is described below with reference to the drawings, in which
Fig. 1 eine schematische Querschnittsansicht durch ein in einer Tischplatte befestigtes Trocknermodul zeigt,1 shows a schematic cross-sectional view through a dryer module fastened in a table top,
Fig. 2 eine gegenüber der Ansicht der Fig. 1 um 90° gedrehte Querschnittsansicht des Trocknermoduls zeigt,FIG. 2 shows a cross-sectional view of the dryer module rotated by 90 ° with respect to the view of FIG. 1,
Fig. 3 eine Ansicht des erfindungsgemäßen Trocknermoduls von unten ist, und3 is a bottom view of the dryer module according to the invention, and
Fig. 4 einen Querschnitt eines verwendeten Filterträgers zeigt.Fig. 4 shows a cross section of a filter carrier used.
In der in der Fig. 1 dargestellten bevorzugten Ausführungsform werden zwei Tangentiallüfter 1 an ihren Längsseiten gekoppelt, um einen möglichst homogenen Luftstrom zu erhalten. Es ergibt sich so ein gleichmäßiger Luftstrom über die gesamte Fläche des zu trocknenden Filterträgers (nicht dargestellt) . Die Austrittsfläche des Luftschachts 4, die in der bevorzugten Ausführungsform 8 cm x 12,5 cm beträgt, ist etwas größer als die zu trocknende Grundfläche des Filterträgers. Der Luftstrom beträgt maximal 2x80 m3/h und kann über einen Vorschalttransformator (nicht dargestellt) über die Umdrehungsgeschwindigkeit der Motore 3 des Paares der Tangentiallüfter 1 geregelt werden.In the preferred embodiment shown in FIG. 1, two tangential fans 1 are coupled on their long sides in order to obtain an air flow that is as homogeneous as possible. This results in an even air flow over the entire surface of the material to be dried Filter carrier (not shown). The outlet area of the air shaft 4, which is 8 cm x 12.5 cm in the preferred embodiment, is somewhat larger than the base area of the filter carrier to be dried. The air flow is a maximum of 2x80 m 3 / h and can be regulated via a series transformer (not shown) via the rotational speed of the motors 3 of the pair of tangential fans 1.
Der rechteckige Luftschacht 4 ist so konstruiert, daß der Luftstrom, einer Düse 6 gleich, zur Austrittsöffung hin gebündelt wird. Er ist aus 2 mm starkem Eisenblech gefertigt und zu einer geschlossenen Form verlötet. An dessem unteren Bereich befindet sich eine Aufnahme für eine Heizung 5. Darunter sind die beiden Tangentiallüfter 1 befestigt. Der obere Teil des Luftschachts 4 ist in einer entsprechend dimensionierten Aussparung einer Tischplatte 7 befestigt und somit im Arbeitsbereich an genau definierter Position fixiert. Die Heizelemente 5a, 5b und die Lüfterelemente 1 befinden sich durch diese Anordnung unter dem Arbeitstisch 7. Die Öffnung des Luftschachtes 4 ist vorzugsweise ebenerdig mit der Arbeitsfläche eines Robotertischs (nicht dargestellt) im Falle der Verwendung des Trocknermoduls in einem automatisierten Verfahren.The rectangular air duct 4 is constructed in such a way that the air flow, like a nozzle 6, is bundled towards the outlet opening. It is made of 2 mm thick iron sheet and soldered to a closed shape. At its lower area there is a receptacle for a heater 5. The two tangential fans 1 are fastened underneath. The upper part of the air duct 4 is fastened in a correspondingly dimensioned recess in a table top 7 and is thus fixed in the work area at a precisely defined position. As a result of this arrangement, the heating elements 5a, 5b and the fan elements 1 are located under the work table 7. The opening of the air shaft 4 is preferably at ground level with the work surface of a robot table (not shown) in the case of using the dryer module in an automated process.
Die Heizung 5 besteht in der bevorzugten Ausführungsform aus zwei Heizelementen 5a, 5b, wie dies in den Figuren 2 und 3 deutlicher werden wird. Diese Heizelemente 5a, 5b sind über der Austrittsöffnung der Tangentiallüfter 1 befestigt. Jeweils zwei Multimicalochplatten werden als Träger für einen Heizdraht verwendet, der in Wendeln von ca. 3 mm Abstand in den Luftstrom eingespannt wird und diesen dadurch gleichmäßig erwärmt. Die Wendel sind dabei so beschaffen, daß die Umkehrschlaufe außerhalb des Luftstroms zu liegen kommt, die parallel verlaufenden Heizdrähte hingegen senkrecht zu den Multi icalochplatten angeordnet sind.In the preferred embodiment, the heater 5 consists of two heating elements 5a, 5b, as will become clearer in FIGS. 2 and 3. These heating elements 5a, 5b are fastened over the outlet opening of the tangential fan 1. Two multimicaloch plates each are used as a support for a heating wire, which is clamped in coils at a distance of approx. 3 mm in the air flow and thereby heats it evenly. The helixes are designed so that the reverse loop comes to lie outside the air flow, the parallel ones Heating wires, however, are arranged perpendicular to the multi icalochplatten.
Der Filterständer, bestehend aus einem Aufnahmeelement 11 und Stützelementen 10, ist so ausgelegt, daß er zentrierende Elemente (dargestellt in der Fig. 3) an seinen Kontaktstellen zum Filterträger (nicht dargestellt) aufweist. Insbesondere sind 30° Phasen an den Berührungsbereichen zu den Abstandshülsen (siehe Fig. 4) eines Filterträgers zu nennen. Der Filterständer umfaßt in der bevorzugten Ausführungsform eine als Aufnahmeelement wirkende obere Trägerplatte oder Zentrierplatte 10, welche die Zentrierelemente, nämlich Aussparungen für die Abstandshülsen und die Auflagefläche für den Rand des Filterträgers aufweist. An dieser Zentrierplatte sind in den vier Eckpunkten 4,5 cm hohe Stützelemente 10 befestigt, die an ihrem unteren Ende jeweils einen Befestigungsbolzen 8 von 0,3 cm aufweisen. Diese Bolzen 9 fixieren den Filterträgerständer exakt über den Luftaustrittsschacht 4 des Trocknermoduls, indem sie in entsprechende Bohrungen einer auf dem Arbeitstisch angeordneten Platte 8 zur Befestigung des Ständers eingreifen. In der bevorzugten Ausführungsform ist die Platte 8 aus Acryl . Nach der Einstellung des Filterträgers, der in Fig. 4 beschrieben ist, befinden sich dessen Auslaßdüsen ca 3 cm über der Oberkante des Luftschachts .The filter stand, consisting of a receiving element 11 and support elements 10, is designed such that it has centering elements (shown in FIG. 3) at its contact points with the filter carrier (not shown). In particular, 30 ° phases at the contact areas to the spacer sleeves (see FIG. 4) of a filter carrier should be mentioned. In the preferred embodiment, the filter stand comprises an upper carrier plate or centering plate 10, which acts as a receiving element and which has the centering elements, namely recesses for the spacer sleeves and the contact surface for the edge of the filter carrier. On this centering plate, 4.5 cm high supporting elements 10 are fastened in the four corner points, each of which has a fastening bolt 8 of 0.3 cm at its lower end. These bolts 9 fix the filter support stand exactly over the air outlet shaft 4 of the dryer module by engaging in corresponding bores in a plate 8 arranged on the work table for fastening the stand. In the preferred embodiment, plate 8 is made of acrylic. After setting the filter carrier, which is described in Fig. 4, its outlet nozzles are about 3 cm above the upper edge of the air duct.
Die Ansteuerung der Lüfterelemente 1 sowie die Freischaltung der Heizelemente 5a, 5b erfolgen getrennt über ein Controllerinterface. Somit kann nach Beendigung des Trocknungsvorgangs zuerst die Heizung 5 abgeschaltet werden, so daß nach zeitlichem Versatz die nun kühlend auf die Heizdrähte einwirkende Lüftung beendet wird. Zur Temperaturmessung ist in der Düse 6 eine seitliche Aussparung 13 zur Aufnahme eines Temperaturfühlers vorgesehen. Die Heizelelmente 5a, 5b wird über eine Temperaturschwellwertschaltung gesteuert .The control of the fan elements 1 and the activation of the heating elements 5a, 5b take place separately via a controller interface. Thus, after the drying process has ended, the heater 5 can first be switched off, so that the ventilation, which is now cooling on the heating wires, is ended after a time delay. For temperature measurement, there is a lateral recess 13 in the nozzle 6 for receiving a temperature sensor intended. The heating elements 5a, 5b are controlled via a temperature threshold circuit.
Fig. 2 zeigt ebenfalls das erfindungsgemäße Trocknermodul in einer Querschnittsansicht, die jedoch gegenüber der Ansicht der Fig. 1 um 90° um die vertikale Symmetrieachse gedreht ist. Zu erkennen sind die beiden parallel angeordneten Tangentiellüfterwalzen 1, die von entsprechenden Motoren 3 angetrieben werden. In dem Luftschacht 4 sind die beiden Heizelemente 5a, 5b der Heizung 5 parallel und fast aneinanderstoßend angeordnet. Darüberliegend ist die Düse 6 als Bestandteil des Schachts 4 zur Bündelung der trockenenden Luft ausgebildet. In der hier dargestellten Ausführungsform ist das Trocknermodul in der Öffnung einer Tischplatte 7 o.a. befestigt. Auf der Tischplatte 7 ist eine Platte 8, vorzugsweise aus Acryl oder einem ähnlichen Werkstoff, angeordnet, die den Filterträgerständer bestehend aus den Stützelementen 9 und der Zentrierplatte 11 trägt, wobei der Filterträgerständer mittels Befestigungsbolzen 9 in der Platte 8 fixiert und ausgerichtet ist.FIG. 2 also shows the dryer module according to the invention in a cross-sectional view, which, however, is rotated by 90 ° around the vertical axis of symmetry compared to the view in FIG. 1. The two tangential fan rollers 1, which are arranged in parallel and are driven by corresponding motors 3, can be seen. In the air duct 4, the two heating elements 5a, 5b of the heater 5 are arranged in parallel and almost abutting one another. Above this, the nozzle 6 is designed as part of the shaft 4 for bundling the drying air. In the embodiment shown here, the dryer module is in the opening of a table top 7 or the like. attached. On the table top 7, a plate 8, preferably made of acrylic or a similar material, is arranged, which supports the filter support stand consisting of the support elements 9 and the centering plate 11, the filter support stand being fixed and aligned in the plate 8 by means of fastening bolts 9.
Fig. 3 zeigt das oben beschrieben Trocknermodul in einer Ansicht von oben. Es ist dabei zu berücksichtigen, daß in der bevorzugten Ausführungsform die Platte 8 aus Acryl besteht und damit durchsichtig ist. Dargestellt sind zwei Tangentiallüfter 1 mit Antriebsverbindungen 2 und Motoren 3, die Heizelelemente 5a und 5b im Luftschacht 4, der eine Düse 6 bildet. Die Zentrierplatte 11 weist vier Zentrierschächt 12 zur Aufnahme entsprechend geformter Abstandshülsen des Filterträgers auf, die in der Fig. 4 dargestellt sind . Durch diese Zentrierschächte 12 wird eine Ausrichtung des Filterträgers erzwungen.3 shows the dryer module described above in a view from above. It should be noted that in the preferred embodiment the plate 8 is made of acrylic and is therefore transparent. Shown are two tangential fans 1 with drive connections 2 and motors 3, the heating elements 5a and 5b in the air shaft 4, which forms a nozzle 6. The centering plate 11 has four centering chutes 12 for receiving correspondingly shaped spacer sleeves of the filter carrier, which are shown in FIG. 4. An alignment of the filter carrier is forced through these centering shafts 12.
Fig. 4 zeigt zur Erläuterung einen Querschnitt durch einen üblicherweise verwendeten Filterträger 14, der aus o einer Vielzahl von Filterelementen 15 gebildet wird, die auf bekannte Weise miteinander verbunden sind. Üblicherweise hat der Filterträger in Draufsicht eine rechteckige Gestalt. Die äußeren Filterelemente 15 des Filterträger 14 weisen Abstandshülsen 17 auf, die zur Zentrierung des Filterträgers 14 in einer automatisierten Umgebung dienen. Hier dienen sie zur Zentrierung in den Zentrierschächten 12 der Zentrierplatte 11. Ferner weist der Filterträger 14 eine als Kragen ausgeformte umlaufende Wandung 18 auf. Beim Einführen des Filterträgers in die Zentrierplatte 11 des Trocknermoduls greifen zuerst die Abstandshülsen 17 in die Zentrierschächte 12 ein und der Filterträger 14 wird beispielsweise durch einen Roboter solange nach unten bewegt, bis die Wandung 18 auf die Zentrierplatte 11 aufsetzt und der Filterträger 14 so seine Endposition einnimmt. Danach wird das Trocknermodul in Betrieb genommen. FIG. 4 shows a cross section through a commonly used filter carrier 14 for explanation o a plurality of filter elements 15 is formed, which are connected to one another in a known manner. The filter carrier usually has a rectangular shape in plan view. The outer filter elements 15 of the filter carrier 14 have spacer sleeves 17 which serve to center the filter carrier 14 in an automated environment. Here they serve for centering in the centering shafts 12 of the centering plate 11. Furthermore, the filter carrier 14 has a circumferential wall 18 shaped as a collar. When the filter carrier is inserted into the centering plate 11 of the dryer module, the spacer sleeves 17 first engage in the centering shafts 12 and the filter carrier 14 is moved downward, for example by a robot, until the wall 18 touches the centering plate 11 and the filter carrier 14 thus reaches its end position occupies. Then the dryer module is put into operation.
BezugszeichenlisteReference list
Tangentiallüfterwalze Antriebsverbindung Motor Luftschacht Heizung Heizelement Düse Tischplatte Acrylplatte Befestigungsbolzen Ständer Zentrierplatte Zentrierschacht Temperaturfühleraufnahme Filterträger Filterelement Filterelementdüse Abstandshülse Wandung Tangential fan roller Drive connection Motor Air shaft Heating Heating element Nozzle Table top Acrylic plate Fastening bolt Stand Centering plate Centering shaft Temperature sensor holder Filter carrier Filter element Filter element nozzle Spacer sleeve wall

Claims

Patentansprüche claims
1. Trocknermodul zum Trocknen von Filterträgern bei der DNA-Präparation, dadurch gekennzeichnet, daß das Trocknermodul ein Aufnahmeelement (11) zur Aufnahme des Filterträgers und mindestens ein Lüfterelement (1) aufweist, wobei das Lüfterelement (1) unterhalb des Aufnahmeelements (11) zur Aufnahme des Filterträgers (14) angeordnet ist, so daß der Filterträger (14) von unten angeblasen und getrocknet wird.1. dryer module for drying filter carriers in the DNA preparation, characterized in that the dryer module has a receiving element (11) for receiving the filter carrier and at least one fan element (1), the fan element (1) below the receiving element (11) for Recording the filter carrier (14) is arranged so that the filter carrier (14) is blown from below and dried.
2. Trocknermodul nach Anspruch 1, dadurch gekennzeichnet, daß das Trocknermodul ferner eine Heizung (5) zum Erwärmen der von unten gegen den Filterträger geblasenen Luft aufweist .2. Dryer module according to claim 1, characterized in that the dryer module further comprises a heater (5) for heating the air blown against the filter carrier from below.
3. Trocknermodul nach einem der vorangegangenen Ansprüche, dadurch gekennzeichnet, daß zwischen dem Lüfterelement (1) und dem Aufnahmeelement (11) des Filterträgers (14) ein Luftschacht (4) angeordnet ist, in dem die Heizung (5) angeordnet ist.3. Dryer module according to one of the preceding claims, characterized in that an air shaft (4) is arranged between the fan element (1) and the receiving element (11) of the filter carrier (14), in which the heater (5) is arranged.
4. Trocknermodul nach einem der vorangegangenen Ansprüche, dadurch gekennzeichnet, daß das Lüfterelement4. Dryer module according to one of the preceding claims, characterized in that the fan element
(1) ein Tangentiallüfter ist.(1) is a tangential fan.
5. Trocknermodul nach einem der vorangegangenen Ansprüche, dadurch gekennzeichnet, daß das Lüfterelement5. Dryer module according to one of the preceding claims, characterized in that the fan element
(1) durch einen elektrischen Motor (3) angetrieben wird.(1) is driven by an electric motor (3).
6. Trocknermodul nach einem der vorangegangenen Ansprüche, dadurch gekennzeichnet, daß zwischen dem Luftschacht (4) und dem Aufnahmeelement (11) des Filterträgers (14) eine Düse (6) zur Bündelung des Luftstromes angeordnet ist. 6. Dryer module according to one of the preceding claims, characterized in that between the air shaft (4) and the receiving element (11) of the filter carrier (14), a nozzle (6) is arranged for bundling the air flow.
7. Trocknermodul nach einem der vorangegangenen Ansprüche, dadurch gekennzeichnet, daß das Aufnahmeelement (11) des Filterträgers als Zentrierplatte ausgebildet ist.7. Dryer module according to one of the preceding claims, characterized in that the receiving element (11) of the filter carrier is designed as a centering plate.
8. Trocknermodul nach einem der vorangegangenen Ansprüche, dadurch gekennzeichnet, daß das Trocknermodul in einer Öffnung einer Tischplatte (7) angeordnet ist.8. Dryer module according to one of the preceding claims, characterized in that the dryer module is arranged in an opening of a table top (7).
9. Trocknermodul nach Anspruch 7, dadurch gekennzeichnet, daß die Zentrierplatte (11) zur Aufnahme des Filterträgers auf Stützelementen (10) angeordnet ist.9. Dryer module according to claim 7, characterized in that the centering plate (11) for receiving the filter carrier on support elements (10) is arranged.
10. Trocknermodul nach einem der vorangegangenen Ansprüche, dadurch gekennzeichnet, daß das Modul einen Temperaturfühler aufweist.10. Dryer module according to one of the preceding claims, characterized in that the module has a temperature sensor.
11. Trocknermodul nach Anspruch 10, dadurch gekennzeichnet, daß das Modul eine elektrische Steuerung zur Steuerung der mindestens einen Motors (3) und der Heizung (5) aufweist, wobei die Heizung als Funktion der vom Temperaturfühler gemessenen Temperatur gesteuert wird.11. Dryer module according to claim 10, characterized in that the module has an electrical control for controlling the at least one motor (3) and the heater (5), the heater being controlled as a function of the temperature measured by the temperature sensor.
12. Trocknermodul nach Anspruch 11, dadurch gekennzeichnet, daß die Steuerung mit einem Delay versehen ist, der bewirkt, daß im Falle eines Abschaltens des Trocknermoduls das mindestens eine Lüfterelement (1) zeitlich nach dem Abschalten der Heizung (5) abgeschaltet wird.12. Dryer module according to claim 11, characterized in that the control is provided with a delay which, in the event that the dryer module is switched off, the at least one fan element (1) is switched off in time after the heating (5) has been switched off.
13. Verwendung des Trocknermoduls nach einem der Ansprüche 1 - 12 in einer automatisierten Umgebung.13. Use of the dryer module according to one of claims 1-12 in an automated environment.
14. Verwendung des Trocknermoduls nach einem der Ansprüche 1 - 12 als Stand-alone-Gerät . 14. Use of the dryer module according to one of claims 1-12 as a stand-alone device.
EP98940128A 1997-07-03 1998-07-03 Drying module Expired - Lifetime EP0993586B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19728496 1997-07-03
DE19728496A DE19728496A1 (en) 1997-07-03 1997-07-03 Dryer module
PCT/EP1998/004121 WO1999001708A1 (en) 1997-07-03 1998-07-03 Drying module

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EP0993586A1 true EP0993586A1 (en) 2000-04-19
EP0993586B1 EP0993586B1 (en) 2002-01-23

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AT (1) ATE212433T1 (en)
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CA (1) CA2295145A1 (en)
DE (2) DE19728496A1 (en)
DK (1) DK0993586T3 (en)
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KR100487345B1 (en) * 2002-11-26 2005-05-03 엘지전자 주식회사 Dryer and Control Method of Cooling Time for the same
CN106369967A (en) * 2016-10-31 2017-02-01 王月娟 Agricultural fodder dryer

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ES2172183T3 (en) 2002-09-16
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EP0993586B1 (en) 2002-01-23
WO1999001708A1 (en) 1999-01-14
JP2002510383A (en) 2002-04-02
DK0993586T3 (en) 2002-04-22
ATE212433T1 (en) 2002-02-15
PT993586E (en) 2002-07-31
DE19728496A1 (en) 1999-01-07
AU8855398A (en) 1999-01-25
US6226892B1 (en) 2001-05-08

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