EP0960339A1 - Dosage de fragments de collagene de type ii - Google Patents
Dosage de fragments de collagene de type iiInfo
- Publication number
- EP0960339A1 EP0960339A1 EP98902752A EP98902752A EP0960339A1 EP 0960339 A1 EP0960339 A1 EP 0960339A1 EP 98902752 A EP98902752 A EP 98902752A EP 98902752 A EP98902752 A EP 98902752A EP 0960339 A1 EP0960339 A1 EP 0960339A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- antibody
- epitope
- collagen
- epitopes
- ah12l3
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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- KXCLCNHUUKTANI-RBIYJLQWSA-N keratan Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@H](COS(O)(=O)=O)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@H](O[C@@H](O[C@H]3[C@H]([C@@H](COS(O)(=O)=O)O[C@@H](O)[C@@H]3O)O)[C@H](NC(C)=O)[C@H]2O)COS(O)(=O)=O)O[C@H](COS(O)(=O)=O)[C@@H]1O KXCLCNHUUKTANI-RBIYJLQWSA-N 0.000 description 1
- 108010045069 keyhole-limpet hemocyanin Proteins 0.000 description 1
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- 230000006371 metabolic abnormality Effects 0.000 description 1
- 239000003475 metalloproteinase inhibitor Substances 0.000 description 1
- ZAHQPTJLOCWVPG-UHFFFAOYSA-N mitoxantrone dihydrochloride Chemical compound Cl.Cl.O=C1C2=C(O)C=CC(O)=C2C(=O)C2=C1C(NCCNCCO)=CC=C2NCCNCCO ZAHQPTJLOCWVPG-UHFFFAOYSA-N 0.000 description 1
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- 238000011587 new zealand white rabbit Methods 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 description 1
- 229940092253 ovalbumin Drugs 0.000 description 1
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- 229940056360 penicillin g Drugs 0.000 description 1
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- 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
Definitions
- antibodies which bind to a coupled epitope of C- ll f ree obtainable (or obtained by) the process described above.
- Figure 4 shows the characterization of antiserum AH8L1.
- Antiserum AH6L1 was tested in ELISA assays against peptide AH8 (Panel A) and HDC (Panel B). The results are shown for specific antiserum (circles) compared to the pre-immune serum (diamonds) for both antigens.
- Figure 6 shows the characterization of antiserum AH12L3. Antiserum AH12L3 was tested in ELISA assays against peptide AH 12 (Panel A) and HDC (Panel B). The results are shown for specific antiserum (circles) compared to the pre-immune serum (diamonds) for both antigens.
- Figure 8 shows Western immunoblots of bovine chondrocyte lysates.
- the lysates were prepared with (lane 1) or without (lane 2) the addition of bovine type II collagen.
- Panel A is an amido black stain of total protein in the lysates after transfer to nitrocellulose.
- Panels B, C and D are Western immunoblots using antibodies AH8L1 , AH9L2 and AH12L3 respectively. The migration position of the type II collagen ⁇ -chain is shown.
- Figure 14 shows a typical example of non-arthritic femoral head cartilage.
- the pattern of staining was similar to that previously described (1 1 ).
- the interterritorial staining seen with Col2-3/4m and AH12L3 was of a low intensity and restricted mainly to a band of staining at the articular surface ( Figure 14, A and B)
- the extent and intensity of staining around chondrocytes was also much reduced There was virtually a complete absence of staining with AH9L2, in most cases at the same level as the non-immune control ( Figure 14C)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
Abstract
L'invention a pour objet des procédés, des trousses et des réactifs pour doser des fragments de collagène, et des procédés thérapeutiques, pronostiques et diagnostiques utilisant ces derniers.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9702252 | 1997-02-06 | ||
| GBGB9702252.9A GB9702252D0 (en) | 1997-02-06 | 1997-02-06 | Collagen assay |
| PCT/GB1998/000304 WO1998035235A1 (fr) | 1997-02-06 | 1998-01-30 | Dosage de fragments de collagene de type ii |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP0960339A1 true EP0960339A1 (fr) | 1999-12-01 |
Family
ID=10807063
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP98902752A Withdrawn EP0960339A1 (fr) | 1997-02-06 | 1998-01-30 | Dosage de fragments de collagene de type ii |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0960339A1 (fr) |
| JP (1) | JP2001511253A (fr) |
| AU (1) | AU5956098A (fr) |
| GB (1) | GB9702252D0 (fr) |
| WO (1) | WO1998035235A1 (fr) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998026286A2 (fr) * | 1996-12-09 | 1998-06-18 | Osteometer Biotech A/S | Dosages en sandwich de fragments de type i de collagene |
| US6030792A (en) * | 1997-11-13 | 2000-02-29 | Pfizer Inc | Assays for measurement of protein fragments in biological media |
| US6642007B1 (en) | 1998-11-02 | 2003-11-04 | Pfizer Inc. | Assays for measurement of type II collagen fragments in urine |
| US7122635B2 (en) * | 1999-01-06 | 2006-10-17 | University Of Southern California | Method and composition for angiogenesis inhibition |
| CN1379685A (zh) | 1999-07-13 | 2002-11-13 | 南加利福尼亚大学 | 用基于MMP－9和β1整联蛋白的拮抗剂抑制血管发生的新方法和组合物 |
| JP4504608B2 (ja) * | 1999-09-13 | 2010-07-14 | 日水製薬株式会社 | 被測定物質の検出又は測定用キット、及び検出又は測定方法 |
| US7390885B2 (en) | 2001-11-26 | 2008-06-24 | Cell Matrix, Inc. | Humanized collagen antibodies and related methods |
| US7365167B2 (en) | 2001-11-26 | 2008-04-29 | Cell Matrix, Inc. | Humanized collagen antibodies and related methods |
| US7488792B2 (en) | 2002-08-28 | 2009-02-10 | Burnham Institute For Medical Research | Collagen-binding molecules that selectively home to tumor vasculature and methods of using same |
| FR2865034A1 (fr) * | 2004-01-14 | 2005-07-15 | Synarc | Procede d'evaluation de la degradation du tissu cartilagineux d'un individu |
| WO2005106495A1 (fr) * | 2004-05-04 | 2005-11-10 | Pharmacia & Upjohn Company Llc | Biomarqueurs de procollagene ii et leurs methodes |
| EP1948235B1 (fr) * | 2005-11-01 | 2013-08-28 | AbbVie Biotechnology Ltd | Méthodes de détermination de l'efficacité de l'adalimumab chez des sujets ayant une spondylarthrite ankylosante utilisant ctx-ii et mmp3 comme biomarqueurs |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994014070A1 (fr) * | 1992-12-04 | 1994-06-23 | Shriners Hospitals For Crippled Children | Immunodosage pour la mesure du clivage collagenique dans le cartilage |
| US5541295A (en) * | 1993-02-12 | 1996-07-30 | The Board Of Governors For Higher Education State Of Rhode Island And Providence Plantations | Detection of type II collagen and its peptides |
-
1997
- 1997-02-06 GB GBGB9702252.9A patent/GB9702252D0/en active Pending
-
1998
- 1998-01-30 WO PCT/GB1998/000304 patent/WO1998035235A1/fr not_active Application Discontinuation
- 1998-01-30 JP JP53398298A patent/JP2001511253A/ja active Pending
- 1998-01-30 EP EP98902752A patent/EP0960339A1/fr not_active Withdrawn
- 1998-01-30 AU AU59560/98A patent/AU5956098A/en not_active Abandoned
Non-Patent Citations (1)
| Title |
|---|
| See references of WO9835235A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU5956098A (en) | 1998-08-26 |
| GB9702252D0 (en) | 1997-03-26 |
| WO1998035235A1 (fr) | 1998-08-13 |
| JP2001511253A (ja) | 2001-08-07 |
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