EP0946208A2 - Compositions a usages multiples et leurs procedes d'utilisation dans des systemes de desinfection et de nettoyage de lentilles de contact - Google Patents

Compositions a usages multiples et leurs procedes d'utilisation dans des systemes de desinfection et de nettoyage de lentilles de contact

Info

Publication number
EP0946208A2
EP0946208A2 EP97950687A EP97950687A EP0946208A2 EP 0946208 A2 EP0946208 A2 EP 0946208A2 EP 97950687 A EP97950687 A EP 97950687A EP 97950687 A EP97950687 A EP 97950687A EP 0946208 A2 EP0946208 A2 EP 0946208A2
Authority
EP
European Patent Office
Prior art keywords
enzyme
composition
bottle
container
housing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP97950687A
Other languages
German (de)
English (en)
Inventor
Bahram Asgharian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alcon Vision LLC
Original Assignee
Alcon Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alcon Laboratories Inc filed Critical Alcon Laboratories Inc
Publication of EP0946208A2 publication Critical patent/EP0946208A2/fr
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L12/00—Methods or apparatus for disinfecting or sterilising contact lenses; Accessories therefor
    • A61L12/08—Methods or apparatus for disinfecting or sterilising contact lenses; Accessories therefor using chemical substances
    • A61L12/086—Container, accessories or devices therefor

Definitions

  • the present invention relates to the field of contact lens cleaning and disinfecting.
  • this invention is directed to the provision of multi-purpose compositions and
  • compositions methods for the preparation of these compositions.
  • the invention is also directed to methods
  • Patent No. 3,910,296 (Karageozian, et al.).
  • chemical agents include organic anti ⁇
  • microbials such as benzalkonium chloride and chlorhexidine
  • inorganic anti-microbials such as benzalkonium chloride and chlorhexidine
  • proteolytic enzymes and polymeric biguanides or polymeric quaternary ammonium compounds are included in the specification.
  • compositions has been necessary to ensure stability of the enzymes prior to use.
  • liquid enzyme compositions are inherently unstable. When a proteolytic enzyme is placed in
  • the enzyme may lose
  • stabilizing agents can protect enzymes from chemical instability
  • the lens is to be simultaneously cleaned and disinfected. Furthermore, since the amount of
  • liquid enzyme composition placed in a diluting composition is controlled by the user, user error
  • Patent Applications Nos. 92-370197; 92-143718; and 92-243215 describe liquid enzyme
  • compositions for treating contact lenses are provided.
  • Soft contact lenses become soiled by collecting various debris and also
  • compositions wherein the enzyme is in a dilute concentration, and the compositions, therefore,
  • FIG. 1 is a perspective view of a preferred embodiment of the invention.
  • FIG. 2 is a elevation view of a preferred embodiment of the invention.
  • FIG. 3 is an exploded elevation view of a preferred embodiment of the invention.
  • FIG. 4 is an exploded cross-section view of a preferred embodiment of the invention
  • FIG. 5 is a cross-section view about line 5-5 of FIG 2 of a preferred embodiment of the invention.
  • FIG. 6 is a cross-section view about line 5-5 of FIG. 2 of a preferred embodiment of the
  • FIG. 7 is a top plan view of a housing of the invention.
  • FIG. 8 is a bottom plan view of a cap and collar of the invention.
  • the present invention is directed to two-part systems which provide for the generation
  • the present invention is also directed to methods of simultaneously cleaning and
  • the two-part system comprises an
  • enzyme cleaning composition an aqueous composition and one or more anti-microbial
  • the enzyme composition provides a concentrated amount of an enzyme.
  • aqueous composition provides a diluting solution.
  • the anti-microbial agent is contained in
  • the two-part system uses a two-compartment device capable of keeping separate an
  • Still another advantage of this feature is that it eliminates
  • Another feature of the present invention is that the enzyme component is kept separate
  • the two components are combined and mixed aseptically, forming the multi-purpose
  • the multi-purpose composition can then be used for a period of from about 1-3
  • the cleaning and disinfecting compositions of the present invention may utilize
  • compositions and methods of the present invention provide greater than
  • the present invention is directed to the use of a sterile two-part system for the
  • the present invention is also directed to methods of cleaning and disinfecting contact lenses by
  • Part I is a sterile enzyme
  • Part II containing solid (powder or tablet) or liquid composition and the second part (“Part II") is a
  • sterile diluting composition An anti-microbial agent is further required, and may be included
  • the present invention requires the use of a two-compartment device to store and mix
  • Figure 1 illustrates a preferred two-part bottle assembly for use with the two-part/multi-
  • compositions of the present invention are purpose compositions of the present invention.
  • assembly 1 generally comprises bottle 2 and container 4.
  • bottle 2 comprises neck 13, opening 10, external rings 12
  • Bottle 2 is made generally of molded polyethylene, although other materials such as
  • PET polyethyleneterphlalate
  • P/P polypropylene
  • container 4 comprises housing 5, plunger 6, cap 8 and
  • Housing 5 comprises hollow cylinder 14 and cap 16. Cylinder 14 has external
  • Stops 23 are disposed annularly about exterior 38 of cylinder 14 (FIG. 7).
  • Cap 16 has thin cross-sectional thickness 49 about its circumference.
  • Cap 16 has protruding internal
  • cap 16 is coaxially disposed within cap 16 such that top end 15 and bottom
  • Plunger 6 comprises hollow cylinder 24, open end 22, dispensing
  • Open end 22 has tooth 39 and is thinner in cross-sectional thickness than
  • collar 28 has tab 29, spokes 19 and perforation 27 which forms ends 31.
  • components are generally made of molded high density polyethylene or polycarbonate, but
  • ABS butadiene styrene
  • container 4 is put together by first adding enzyme cleaning
  • composition 7 to hollow cylinder 14 of housing 5, ringing collar 28 over cylinder 14, inserting
  • spokes 19 of collar 28 are interspersed between both stops 21 of cap 8 and stops
  • Aqueous composition 9 is added to bottle 2, container 4 is then placed over neck 13,
  • cap 16 is forced down on neck 13 such that rings 20 compress radially against neck 13, and
  • exterior rings 12 compress against interior 47 of cap 16, forming an air-tight seal.
  • collar 28 is first removed from container 4 by screwing cap 8 downward
  • plunger 6 is simultaneously pushed downward causing
  • plunger 6 to descend cylinder 14.
  • membrane 18 of housing 5 sharp
  • composition contained in housing 5 is exposed to interior 1 1 of bottle 2 and falls into aqueous
  • Bottle 2 may then be inverted and shook, thus affecting the
  • composition may now be dispensed through this channel to an appropriate container for
  • a blister pouch and piercing means may be utilized as the
  • the present invention is comprised of two separate compositions which
  • Part I comprises an enzyme and Part II comprises an enzyme
  • the resultant multi-purpose composition may contain various other ingredients.
  • agents but must contain: 1) an anti-microbial agent, 2) an enzyme, 3) a buffering agent, 4) a
  • compositions of the present invention are
  • multi-purpose compositions will be physiologically compatible with the eye.
  • the Part I sterile enzyme composition of the present invention is generally composed of
  • the enzyme composition may be formulated as a
  • Dry powder or tablet compositions may be preferred when the Part I
  • powder composition will include bulking agents to carry the relatively small volume of enzyme
  • Such bulking agents typically include polyols (e.g., mannitol or
  • soribitol polyethylene glycols (molecular weights greater than 1000) and sugars.
  • excipients may include salts such as NaCl, chelating agents such as EDTA, and buffering
  • agents such as Tris.
  • Other additives may include surfactants to ease dispersion and dissolution
  • Preferred enzyme powder compositions comprise mannitol and
  • PEG-5000 polyethylene glycol-5000
  • Enzyme tablet compositions and methods of manufacturing are known in the art.
  • Enzyme tablets require the use of bulking agents and binding agents. Additionally, tablets may
  • effervescing agents such as bicarbonate to expedite dissolution of the tablet into the
  • Liquid enzyme compositions are preferred Part I compositions of the present invention.
  • Enzymes contained in Part I liquid compositions may be solubilized in aqueous
  • compositions or dispersed in non-aqueous compositions are compositions or dispersed in non-aqueous compositions.
  • Aqueous enzyme compositions are generally preferred due to their ease of preparation
  • Aqueous enzyme compositions typically comprise one or more polyol(s) and
  • a borate or boric acid compound a borate or boric acid compound.
  • inventions comprise a 2-3 carbon polyol and a borate or boric acid compound.
  • a 2-3 carbon polyol and a borate or boric acid compound.
  • 2-3 carbon polyol refers to a compound with 2 to 3 carbon atoms and at least two
  • Examples of 2-3 carbon polyols are glycerol, 1,2-propane diol ("propylene
  • glycol 1,3-propane diol and ethylene glycol.
  • Glycerol is the most preferred 2-3 carbon
  • compositions of the present invention include alkali metal salts of borate, boric acid and borax.
  • excipients which may be included in the Part I aqueous enzyme compositions include
  • divalent ions such as calcium
  • enzyme stabilizing organic acids such as benzoic acid and
  • surfactants such as alkylethoxylates.
  • inventions generally comprise a crystalline enzyme uniformly dispersed in a water-soluble
  • organic liquid examples include polyoxyethylenes (e.g., PEG-400) and alkoxy
  • polyoxyethylenes such as methoxy polyethylene glycols.
  • the enzyme is in this composition, in this composition, in
  • the anti-microbial agent(s) of the present invention may be included in any suitable pharmaceutically acceptable medium.
  • the anti-microbial agent(s) of the present invention may be included in any suitable pharmaceutically acceptable medium.
  • Part I enzyme composition are aseptically processed generally by
  • inventions include all enzymes which: (1) are useful in removing deposits from contact lenses;
  • proteolytic enzymes used herein must have at least a partial capability to hydrolyze
  • amylolytic or related activities associated with the proteolytic activity may be neutral,
  • the present invention include, but are not limited to: pancreatin, trypsin, subtilisin, collagenase, keratinase, carboxypeptidase, bromelain, aminopeptidase, elastase, Aspergillo peptidase,
  • pronase E from S griseus
  • dispase from Bacillus polymyxa
  • Microbially derived enzymes such as those derived from Bacillus. Streptomyces. and
  • Aspergillus microorganisms represent a preferred type of enzyme which may be utilized in the
  • subtilisin neutral or slightly alkaline proteases generically called "subtilisin” enzymes.
  • enzymes including those enzymes having proteolytic and mixed
  • proteolytic/lipolytic/amylolytic activity The enzymes contemplated by this invention can be any enzymes contemplated by this invention.
  • the enzymes contemplated by this invention can be any enzymes contemplated by this invention.
  • Preferred genetically modified enzymes include BPN' subtilisin variants, such as those
  • subtilisins and variants include subtilisin Carlsberg, subtilisin PB92, subtilisin 309, subtilisin
  • subtilisin 168 subtilisin DY and truncations, modifications and variants thereof.
  • modifications include “pegylation,” i.e., covalent bonding of polyoxyethylene
  • glycol derivatives to the enzymes as well as monomeric covalent additions to the enzymes
  • compositions of the present invention It is believed that alkylation of hydro lyrically sensitive compounds.
  • Subtilisin and trypsin are preferred enzymes, and genetically modified subtilisin BPN's
  • Subtilisin is derived from
  • Bacillus bacteria and is commercially available from various commercial sources including
  • Subtilisin BPN' variants as described above are genetically modified subtilisins which have
  • Pancreatin is extracted from mammalian pancreas, and is commercially available from
  • Pancreatin USP is a mixture of
  • pancreatin 9X The most preferred form of pancreatin is Pancreatin 9X. As utilized herein, the term
  • Pantcreatin 9X means a filtered (0.2 microns) pancreatin containing nine times the USP
  • the Part I enzyme concentration will depend on various factors, such as: the enzyme or
  • invention may be prepared with initial amounts of enzyme that exceed the amount necessary to
  • Part I enzyme compositions of the present invention will preferably contain one or more
  • enzymes in an amount of from about 100-100,000 PAU/g or 100-100,000 PAU/mL.
  • Part I enzyme compositions will contain an effective amount of one or
  • composition of the present invention is referred to as "an amount effective to clean the
  • concentration in the multi-purpose composition of from about 1-100 PAU/mL of solution
  • proteolytic activity unit or "PAU” is defined as the amount of enzyme activity necessary to generate one microgram (meg) of tyrosine per minute (“meg Tyr/min”), as determined by the
  • casein substrate (0.65% casein w/v) is equilibrated for 10 minutes
  • composition by solubilizing and diluting the Part I composition in PBS buffer.
  • the sample concentration is then determined by comparison to a tyrosine standard curve.
  • Part I concentration is then calculated by taking into account the dilution ratio.
  • the Part II aqueous compositions provide the volume of distilled water necessary for
  • the multi-purpose compositions of the present invention In general, the Part II composition
  • Part I compositions may be ophthalmically compatible solution.
  • Part I compositions may be ophthalmically compatible solution.
  • Part II compositions may provide only a percentage of these ingredients, or none at all.
  • Part II compositions utilized in the present invention may contain various other ingredients
  • components such as suitable buffering agents, chelating and/or sequestering agents and tonicity
  • the Part II compositions may also contain surfactants. As stated above, the
  • the Part II compositions may also be included in the Part II compositions.
  • the Part II may also be included in the Part II compositions.
  • the Part II may also be included in the Part II compositions.
  • the Part II may also be included in the Part II compositions.
  • compositions will contain one or more anti-microbial agents (e.g., PHMB or polyquaternium-
  • anti-microbial agents e.g., PHMB or polyquaternium-
  • a buffer e.g., borate
  • citrates e.g., citrates
  • tonicity agents e.g., NaCl, sugars
  • a chelating agent e.g., sodium citrate
  • EDTA EDTA
  • surfactants e.g., block copolymers
  • compositions such as amino alcohols and alkylamines, may also be used.
  • Preferred Part II compositions comprise polyquaternium-1, sodium borate, boric acid,
  • MAPDA MAPDA
  • lenses including rigid gas-permeable (“RGP”) lenses and soft lenses.
  • RGP rigid gas-permeable
  • the multi-purpose compositions may be optimized to effect maximum
  • contact lenses are electronegatively charged, they tend to bind enzymes with high isoelectric
  • the cleaning obtained with the liquid enzyme compositions of the present invention is a
  • the soaking times utilized will generally vary from about 1 hour to
  • the cleaning methods of the present invention involve the use of a small amount of the
  • the present invention may vary, depending on the enzyme concentration, as described above, as
  • the nature of the lens care regimen e.g., the frequency
  • cleaning agents e.g., surfactants.
  • surfactants e.g., surfactants
  • Part I composition of a Part I compostion will be added to about 120 mL of a Part II composition, although greater
  • anti-microbial activity of anti-microbial agents The anti-microbial activity of disinfecting
  • polymeric quaternary ammonium compounds such as polyquaternium-1
  • polymeric quaternary ammonium compounds and particularly those of Formula
  • the multi-purpose compositions of the present invention will have tonicities/osmolalities in the range of hypotonic to isotonic, and more
  • mOs/kg milliOsmoles per kilogram
  • 300 mOs/kg is particularly preferred, and an osmolality of about 220 mOs/kg is most preferred.
  • the cleaning and disinfecting methods of the present invention utilize a multi-purpose
  • composition of the present invention containing an anti-microbial agent.
  • Anti-microbial agents include anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial agents, anti-microbial
  • polymeric anti-microbial agent refers to any organic compound
  • polymeric anti-microbial agents include: polyquaternium-1, which is a polymeric quaternary
  • PAPB polymeric biguanide
  • suitable in the methods of the present invention include: other quaternary ammonium
  • anti-microbial agents used herein are preferably employed in the absence of mercury-
  • the most preferred anti-microbial agents are polymeric quaternary ammonium
  • R] and R 2 can be the same or different and are selected from:
  • polyquaternium-1 which is also known as
  • Polyquaternium-1 is a mixture of the above
  • anti-microbial agents are utilized in the methods of the present
  • agent employed will vary, depending on factors such as the type of lens care regimen in which
  • the method is being utilized.
  • the use of an efficacious daily cleaner in the lens is being utilized.
  • the use of an efficacious daily cleaner in the lens is being utilized.
  • care regimen may substantially reduce the amount of material deposited on the lenses
  • microorganisms including microorganisms, and thereby lessen the amount of anti-microbial agent required to
  • the type of lens being treated e.g., "hard” versus “soft” lenses
  • the type of lens being treated may also be used.
  • (I) is about 0.001% by weight.
  • the methods of the present invention will typically involve adding about 2-10 mL of a
  • the contact lenses are first rubbed with a multi ⁇
  • the lens will typically be soaked overnight, but shorter or longer
  • composition of the present invention are described below:
  • liquid enzyme composition represents a preferred enzyme composition
  • Calcium chloride and boric acid are dispersed in 30% of the volume of purified water.
  • PEG and glycerol are then added.
  • the pH of the solution is adjusted, and the enzyme is then added.
  • composition is the sterile filtered using a 0.2 ⁇ m filter.
  • the ingredients are dissolved with 90% of the volume of purified water, the pH is
  • composition is then sterile
  • Preferred amounts include 1 mL of
  • the enzyme composition and 120 mL of the aqueous composition.
  • liquid enzyme composition of the present invention The following is an example of a liquid enzyme composition of the present invention:
  • the above formulation is prepared by first sequentially mixing propylene glycol,
  • the enzyme composition is then sterile filtered (0.2 ⁇ m filter).
  • optimal pH of the above formulation will be in the range of 5-7; a pH of 6 is most preferred.
  • the tablets are generally prepared by first mixing the appropriate amounts of each of
  • the tablets may then be sterilized by the method of
  • the enzyme and lactose are dissolved in water (lg of enzyme/lactose per 1 mL of water) and sterile filtered using a 0.2 ⁇ m filter. The sterile enzyme solution as then aseptically lyophilized.
  • Part II compositions are prepared in a similar way as those of Example 1, above.
  • the Part I and II compositions described in the Examples above will be combined, stored and mixed in a single bottle assembly in various quantities. In general, preferred amounts will be:
  • Part i 1 g of powder or 1 tablet of a solid enzyme composition, or 1 ml of liquid enzyme composition. Part II: about 120 ml
  • the preferred enzyme activity in the final multi-purpose solution will be about 5-25 PAU/ml.

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  • Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Chemical & Material Sciences (AREA)
  • Detergent Compositions (AREA)
  • Eyeglasses (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne des ensembles flacons à deux compartiments particulièrement adaptés à la préparation de compositions à usages multiples renfermant une enzyme et un désinfectant acceptables d'un point de vue ophtalmologique. Elle concerne également des procédés de préparation desdites compositions ainsi que des procédés impliquant l'utilisation de ces compositions pour le nettoyage et la désinfection de lentilles de contact.
EP97950687A 1996-12-13 1997-11-24 Compositions a usages multiples et leurs procedes d'utilisation dans des systemes de desinfection et de nettoyage de lentilles de contact Ceased EP0946208A2 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3283996P 1996-12-13 1996-12-13
US32839P 1996-12-13
PCT/US1997/021579 WO1998025650A2 (fr) 1996-12-13 1997-11-24 Compositions a usages multiples et leurs procedes d'utilisation dans des systemes de desinfection et de nettoyage de lentilles de contact

Publications (1)

Publication Number Publication Date
EP0946208A2 true EP0946208A2 (fr) 1999-10-06

Family

ID=21867099

Family Applications (1)

Application Number Title Priority Date Filing Date
EP97950687A Ceased EP0946208A2 (fr) 1996-12-13 1997-11-24 Compositions a usages multiples et leurs procedes d'utilisation dans des systemes de desinfection et de nettoyage de lentilles de contact

Country Status (8)

Country Link
EP (1) EP0946208A2 (fr)
JP (1) JP2001508675A (fr)
KR (1) KR20000057525A (fr)
CN (1) CN1251533A (fr)
AU (1) AU721951B2 (fr)
CA (1) CA2274753A1 (fr)
NZ (1) NZ336203A (fr)
WO (1) WO1998025650A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6358897B1 (en) * 1996-06-07 2002-03-19 Alcon Laboratories, Inc. Alkyl trypsin compositions and methods of use in contact lens cleaning and disinfecting systems
US6228323B1 (en) * 1996-12-13 2001-05-08 Alcon Laboratories, Inc. Multi-purpose compositions containing an alkyl-trypsin and methods of use in contact lens cleaning and disinfecting
US6814088B2 (en) 1999-09-27 2004-11-09 The Procter & Gamble Company Aqueous compositions for treating a surface
JP4286480B2 (ja) * 2001-09-17 2009-07-01 株式会社メニコン 殺菌液
TWI322828B (en) * 2002-12-23 2010-04-01 Alcon Inc Use of multifunctional surface active agents to clean contact lenses
US20050119141A1 (en) * 2003-12-01 2005-06-02 Irene Quenville Stability enhancement of solutions containing antimicrobial agents
US20070264226A1 (en) * 2006-05-10 2007-11-15 Karagoezian Hampar L Synergistically enhanced disinfecting solutions

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8812109D0 (en) * 1988-05-23 1988-06-29 Ifejika C Method for daily cleaning & protein deposit removal for contact lenses
CA2009118C (fr) * 1989-02-21 1996-02-27 Mary F. Mowrey-Mckee Methode et compose pour nettoyer et desinfecter les verres de contact
CA2041871C (fr) * 1990-05-09 2000-07-11 Ruth A. Rosenthal Nettoyage et desinfection des lentilles corneennes a l'aide d'une association d'enzymes et de composes polymeriques d'ammonium quaternaire
US5356555A (en) * 1992-09-14 1994-10-18 Allergan, Inc. Non-oxidative method and composition for simultaneously cleaning and disinfecting contact lenses using a protease with a disinfectant
US5723421A (en) * 1995-06-07 1998-03-03 Alcon Laboratories, Inc. Stable liquid enzyme compositions and methods of use in contact lens cleaning and disinfecting systems
US5605661A (en) * 1995-08-18 1997-02-25 Alcon Laboratories, Inc. Methods of using liquid enzyme compositions containing mixed polyols
US5718895A (en) * 1995-11-16 1998-02-17 Alcon Laboratories, Inc. Enzymes with low isoelectric points for use in contact lens cleaning

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9825650A2 *

Also Published As

Publication number Publication date
AU5362498A (en) 1998-07-03
CN1251533A (zh) 2000-04-26
CA2274753A1 (fr) 1998-06-18
KR20000057525A (ko) 2000-09-25
WO1998025650A3 (fr) 1998-11-12
AU721951B2 (en) 2000-07-20
NZ336203A (en) 2000-01-28
JP2001508675A (ja) 2001-07-03
WO1998025650A2 (fr) 1998-06-18

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