EP0927756A4 - Protein having cellulase activities and process for producing the same - Google Patents

Protein having cellulase activities and process for producing the same

Info

Publication number
EP0927756A4
EP0927756A4 EP97907324A EP97907324A EP0927756A4 EP 0927756 A4 EP0927756 A4 EP 0927756A4 EP 97907324 A EP97907324 A EP 97907324A EP 97907324 A EP97907324 A EP 97907324A EP 0927756 A4 EP0927756 A4 EP 0927756A4
Authority
EP
European Patent Office
Prior art keywords
protein
cellulase
producing
activity
same
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP97907324A
Other languages
German (de)
French (fr)
Other versions
EP0927756A1 (en
EP0927756B1 (en
Inventor
Takashi Yamanobe
Manabu Watanabe
Toru Hamaya
Naomi Sumida
Kaoru Aoyagi
Takeshi Murakami
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Meiji Seika Kaisha Ltd
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Meiji Seika Kaisha Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology, Meiji Seika Kaisha Ltd filed Critical Agency of Industrial Science and Technology
Publication of EP0927756A1 publication Critical patent/EP0927756A1/en
Publication of EP0927756A4 publication Critical patent/EP0927756A4/en
Application granted granted Critical
Publication of EP0927756B1 publication Critical patent/EP0927756B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The object of the present invention is to analyze the amino acid sequence of the protein constituting the cellulase system, to clone the gene coding for the components of the cellulase system, and to establish a technique of inserting a clonal gene into the above strain thereby to produce cellulase having enhanced avicellase activity. The present invention relates to a protein having a part or the whole of the amino acid sequence depicted in the sequence listing under SEQ ID NO:1 and possessing cellulase activity, a DNA coding for the protein, an expression vector containing the DNA, a microorganism as transformed by the expression vector, and a process for producing a protein having enhanced cellulase activity by using the microorganism.
EP97907324A 1996-03-14 1997-03-14 Protein having cellulase activities and process for producing the same Expired - Lifetime EP0927756B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP8447996 1996-03-14
JP8447996 1996-03-14
PCT/JP1997/000824 WO1997033982A1 (en) 1996-03-14 1997-03-14 Protein having cellulase activities and process for producing the same

Publications (3)

Publication Number Publication Date
EP0927756A1 EP0927756A1 (en) 1999-07-07
EP0927756A4 true EP0927756A4 (en) 2001-10-10
EP0927756B1 EP0927756B1 (en) 2006-07-26

Family

ID=13831788

Family Applications (1)

Application Number Title Priority Date Filing Date
EP97907324A Expired - Lifetime EP0927756B1 (en) 1996-03-14 1997-03-14 Protein having cellulase activities and process for producing the same

Country Status (7)

Country Link
US (1) US6127160A (en)
EP (1) EP0927756B1 (en)
JP (1) JP3962805B2 (en)
AT (1) ATE334195T1 (en)
AU (1) AU1941597A (en)
DE (1) DE69736389T8 (en)
WO (1) WO1997033982A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001096382A2 (en) 2000-06-15 2001-12-20 Prokaria Ehf. Thermostable cellulase
WO2005003319A2 (en) 2003-07-02 2005-01-13 Diversa Corporation Glucanases, nucleic acids encoding them and methods for making and using them
WO2005100582A2 (en) 2004-03-25 2005-10-27 Novozymes Inc. Methods for degrading or converting plant cell wall polysaccharides
CA2861310A1 (en) * 2005-03-15 2006-09-28 Bp Corporation North America Inc. Cellulases, nucleic acids encoding them and methods for making and using them
WO2007094852A2 (en) 2006-02-10 2007-08-23 Verenium Corporation Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
WO2007100897A2 (en) * 2006-02-27 2007-09-07 Edenspace System Corporation Energy crops for improved biofuel feedstocks
EP2444413A1 (en) * 2006-08-04 2012-04-25 Verenium Corporation Methods for oil or gas well drilling, washing and/or fracturing
NZ578309A (en) 2007-01-30 2012-06-29 Verenium Corp Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
WO2010096510A2 (en) 2009-02-17 2010-08-26 Edenspace Systems Corporation Tempering of cellulosic biomass
US20120079627A1 (en) 2009-05-29 2012-03-29 Edenspace Systems Corporation Plant gene regulatory elements
DK2468859T3 (en) 2009-08-20 2018-03-12 Meiji Seika Pharma Co Ltd HIS UNKNOWN PROTEIN WITH B-GLUCOSIDASE ACTIVITY AND ITS USE
BR112012024959A2 (en) 2010-03-31 2015-09-22 Meiji Seika Pharma Co Ltd new cellulose gene
WO2013166312A1 (en) * 2012-05-02 2013-11-07 The Trustees Of Columbia University In The City Of New York Biofuel production enzymes and uses thereof
JP6106738B2 (en) * 2015-11-20 2017-04-05 Ｍｅｉｊｉ Ｓｅｉｋａファルマ株式会社 Novel cellulase gene

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0312121A2 (en) * 1984-04-13 1989-04-19 OY ALKO AB (Alko Ltd.) Yeast strains producing cellulolytic enzymes and methods and means for constructing them

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4562150A (en) * 1983-03-09 1985-12-31 Agency Of Industrial Science And Technolgy Method for manufacture of cellulase
JPS6043954B2 (en) * 1983-03-09 1985-10-01 工業技術院長 Method for producing cellulase
JPH06277088A (en) * 1993-03-30 1994-10-04 Toyo Eng Corp Production and purification of recombinant protein

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0312121A2 (en) * 1984-04-13 1989-04-19 OY ALKO AB (Alko Ltd.) Yeast strains producing cellulolytic enzymes and methods and means for constructing them

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE AAGENESEQ [online] 21 October 1991 (1991-10-21), KNOWLES, J ET AL: "yeast strains producing cellulolytic enzymes and methods and means for constructing them", XP002174199, retrieved from EBI Database accession no. AAP50308 *
DATABASE EMBL [online] 11 August 1994 (1994-08-11), SHEPPARD P.O. ET AL: "The use of conserved cellulase family-specific...", XP002174198, retrieved from EBI Database accession no. L29377 *
HARKKI A ET AL: "GENERIC ENGINEERING OF TRICHODERMA TO PRODUCE STRAINS WITH NOVEL CELLULASE PROFILES", ENZYME AND MICROBIAL TECHNOLOGY, STONEHAM, MA, US, vol. 13, no. 3, 1 March 1991 (1991-03-01), pages 227 - 233, XP000654069, ISSN: 0141-0229 *
SHEPPARD P O ET AL: "THE USE OF CONSERVED CELLULASE FAMILY-SPECIFIC SEQUENCES TO CLONE CELLULASE HOMOLOGUE CDNAS FROM FUSARIUM OXYSPORUM", GENE,ELSEVIER BIOMEDICAL PRESS. AMSTERDAM,NL, vol. 150, L29377, 2 December 1994 (1994-12-02), pages 163 - 167, XP000647707, ISSN: 0378-1119 *
YAMANOBE T ET AL: "Purification of endo-1,4-beta-glucanase components from fungal strain Y - 94;", AGRIC.BIOL.CHEM.;(1990) 54, 2, 301-07 CODEN: ABCHA6, XP002174168 *

Also Published As

Publication number Publication date
DE69736389D1 (en) 2006-09-07
WO1997033982A1 (en) 1997-09-18
DE69736389T2 (en) 2007-08-02
AU1941597A (en) 1997-10-01
EP0927756A1 (en) 1999-07-07
JP3962805B2 (en) 2007-08-22
US6127160A (en) 2000-10-03
EP0927756B1 (en) 2006-07-26
ATE334195T1 (en) 2006-08-15
DE69736389T8 (en) 2007-11-22

Similar Documents

Publication Publication Date Title
EP0927756A4 (en) Protein having cellulase activities and process for producing the same
ES2150795T3 (en) PHYTASE OF PENIOPHORA.
AU7550098A (en) System for expressing hyperthermostable protein
AU6414196A (en) A modified enzyme with lipolytic activity
ZA967173B (en) Purified coprinus laccases and nucleic acids and encoding same
AU7587696A (en) Differentiation-suppressive polypeptide
CA2180103A1 (en) Gene encoding adseverin
BR0015114A (en) Nucleic acid and amino acid sequences, nucleic acid construction, vector, microorganism, l-pantolactone hydrolase, and process for preparing d-pantolactone
BG102973A (en) LIGAND RET (RetL) FOR THE STIMULATION OF THE NERVE AND RENAL GROWTH
EP0821064A3 (en) Recombinant fructosyl amino acid oxidase
EP0967283A4 (en) Genes encoding proteins having transglycosylation activity
CA2260365A1 (en) Differentiation-suppressive polypeptide
HRP960078B1 (en) Dna sequence coding for a protein of a. thaliana having a delta-5, 7 sterol, delta- 7 reductase activity, delta-7-red protein, production process, strains of transformed yeasts, uses
ZA931333B (en) Recombinant plant enzyme.
EP0780471A3 (en) Novel nitrilase gene
EP1757684A3 (en) A 2,6-beta-D-fructan hydrolase enzyme and processes for using the enzyme
HUT65492A (en) Biosynthetic process for the preparation of dedydro-amino acid-remainds and tioether-bridge contaning chemical compounds
IL111608A0 (en) Tyrosinase-activator protein fusion enzyme
EP0469523A3 (en) Cloning and overexpression of glucose-6-phosphate dehydrogenase of leuconostoc dextranicus
EP1123977A4 (en) GENES ENCODING PROTEINS REGULATING pH OF VACUOLES
EP1047710B1 (en) Orotidine 5'-phosphate decarboxylase-gene, gene construct containing said gene and the utilization thereof
WO1999045107A8 (en) Novel calpain and dna encoding the same
NZ501118A (en) Vascular adhesion protein-1 having amine oxidase activity
HUP0003541A2 (en) Expression vector for improved production of polypeptides in yeast
IL158244A0 (en) Plants characterized by enhanced growth and methods and nucleic acid constructs useful for generating same

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 19981013

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

A4 Supplementary search report drawn up and despatched

Effective date: 20010829

AK Designated contracting states

Kind code of ref document: A4

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

17Q First examination report despatched

Effective date: 20020412

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20060726

Ref country code: LI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20060726

Ref country code: IT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT;WARNING: LAPSES OF ITALIAN PATENTS WITH EFFECTIVE DATE BEFORE 2007 MAY HAVE OCCURRED AT ANY TIME BEFORE 2007. THE CORRECT EFFECTIVE DATE MAY BE DIFFERENT FROM THE ONE RECORDED.

Effective date: 20060726

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20060726

Ref country code: CH

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20060726

Ref country code: BE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20060726

Ref country code: AT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20060726

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REF Corresponds to:

Ref document number: 69736389

Country of ref document: DE

Date of ref document: 20060907

Kind code of ref document: P

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20061026

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20061026

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: ES

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20061106

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20061226

NLV1 Nl: lapsed or annulled due to failure to fulfill the requirements of art. 29p and 29m of the patents act
EN Fr: translation not filed
PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed

Effective date: 20070427

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MC

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20070331

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20070314

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20061027

Ref country code: FR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20070511

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20060726

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20070314

REG Reference to a national code

Ref country code: DE

Ref legal event code: R082

Ref document number: 69736389

Country of ref document: DE

Representative=s name: KOTITSCHKE & HEURUNG PARTNERSCHAFT MBB, DE

Ref country code: DE

Ref legal event code: R082

Ref document number: 69736389

Country of ref document: DE

Representative=s name: KOTITSCHKE & HEURUNG PARTNERSCHAFT MBB PATENT-, DE

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20150310

Year of fee payment: 19

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20150311

Year of fee payment: 19

REG Reference to a national code

Ref country code: DE

Ref legal event code: R119

Ref document number: 69736389

Country of ref document: DE

GBPC Gb: european patent ceased through non-payment of renewal fee

Effective date: 20160314

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GB

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20160314

Ref country code: DE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20161001