EP0695220B1 - Pipette a piston - Google Patents

Pipette a piston Download PDF

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Publication number
EP0695220B1
EP0695220B1 EP94911982A EP94911982A EP0695220B1 EP 0695220 B1 EP0695220 B1 EP 0695220B1 EP 94911982 A EP94911982 A EP 94911982A EP 94911982 A EP94911982 A EP 94911982A EP 0695220 B1 EP0695220 B1 EP 0695220B1
Authority
EP
European Patent Office
Prior art keywords
pipette
piston
cylinder
gripping means
downward
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP94911982A
Other languages
German (de)
English (en)
Other versions
EP0695220A1 (fr
Inventor
Jukka TERVAMÄKI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thermo Fisher Scientific Oy
Original Assignee
Labsystems Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Labsystems Oy filed Critical Labsystems Oy
Publication of EP0695220A1 publication Critical patent/EP0695220A1/fr
Application granted granted Critical
Publication of EP0695220B1 publication Critical patent/EP0695220B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type

Definitions

  • the invention relates to the handling of liquids and it concerns a hand-held piston pipette.
  • step pipette In a so-called step pipette the liquid volume drawn into the pipette cylinder is metered out in several smaller doses.
  • the piston rod usually has teeth and the body has a matching striker always pushing the piston downward over the desired distance during a dosing stroke.
  • One such pipette is known, for example, from publication US-4099548 wherein the top end of the body has a thumb-operated push knob.
  • Publication GB-A-2048712 again presents a similar step pipette, wherein in the side of the top part of the body there is a forefinger-operated dosing push button.
  • the striker actuator is a lever journalled in the body in its top part above the striker point.
  • the lever is operated with the thumb.
  • One such step pipette is known, for example, from publication DE-A-2926691 (corresponding to publication GB-A-2057581).
  • This pipette also has a volume control device having a longitudinally moving covering plate above the teeth. The striker always moves over a standard distance, but part of the movement is along the covering plate off the teeth. Thus the distance covered by the piston is dependent on the location of the plate.
  • WO 84/04056 discloses a dosage pipette provided with a piston, a toothed rack connected with the piston, a striking pin resting against the toothed rack, and a dosage lever mounted on the striking pin.
  • a lifting lever is placed at the side of the toothing, with the distance of that lever from the end of the toothed rack being adjustable as desired and that lever permitting the striking pin to push the toothed rack over a specified distance, only.
  • the toothed rack is provided with a second toothing, a cogwheel resting against it and, at the other side, against a second toothed rack whose top end is provided with a shiftable press knob.
  • US-A-3 975 960 discloses a manual fluid sampler which maybe hand-held like a pencil and which comprises a longitudinal body provided with a bore and a chamber extending longitudinally therethrough.
  • a piston connected to a plunger is slidably mounted within the bore and provided with pulling - pushing equipment for moving the piston and plunger upwards or downwards in the bore and chamber.
  • An adjustable control is provided in the bore to adjust the stroke of the piston, and a lever is mounted on the body to operate the plunger through the intermediary of the piston whereby fluid may be drawn into or discharged from the chamber.
  • a stroke - overstroke arrangement is operatively coupled with the lever to cooperate with the control to define the limits of a stroke for the piston.
  • the pipette according to the invention is designed to be gripped like a pencil. Thus liquid can be dosed very precisely with the pipette even to very small places.
  • the pipette is particularly suitable for use in certain laboratory work and in assembly glueing.
  • the pipette may be a step pipette or a one-dose pipette. It may be manually operated or electrically operating.
  • a gripping means attached to a piston for moving the piston downward, but also allowing the piston to be pulled upward.
  • the top of the pipette is taken as the end opposite the liquid outlet nozzle. Hence a downward movement is towards this nozzle, and an upward movement is away from this nozzle.
  • the pipette has a transfer means extending below the gripping means and joined to this and preferably also associated with a separate push button.
  • the pipette body may have a longitudinal gap partly open at least on one side for the cylinder and the piston.
  • the transfer means is spring-operated so that it will always return to its original position after the transfer movement.
  • the transfer means is movable in the longitudinal body direction so that the top position of the transfer means also corresponds with the top position of the gripping means.
  • the transfer means is preferably on one side of the body. Hereby the opposite side of the body may have an open gap through which an exchangeable cylinder and piston can be mounted.
  • the transfer means is also preferably joined to an operating lever articulated to the body and articulated to the transfer means.
  • the pipette also has a stroke length control means.
  • a point vessel into which liquid is drawn can be used at the cylinder end.
  • the cylinder may function at the same time also as a liquid container and both the cylinder and the piston can be exchangeable.
  • the pipette can be provided, for example, with a spring means for pulling the piston upward.
  • a step pipette is preferably only provided with a piston knob for pulling the piston upward by hand.
  • the body is preferably provided with a stop to prevent the piston from escaping from the cylinder.
  • the pipette may have equipment for controlling the liquid volume to be drawn into the pipette.
  • the stroke length control means may have, for example, a stop which moves in the body direction and which limits the upper position of the striker.
  • a spring is connected to the gripping means for pressing down the moving gripping means against the piston rod.
  • the device is also preferably provided with a releaser automatically to release the gripping means from the piston when the gripping means is in its top position in relation to the piston, whereby the piston can be easily pulled up to its top position.
  • the main parts of the pipettes given as examples are body 1, cylinder 2 and therein piston 3 as well as striker 4, which functions as a transfer means, and connected to this gripping means 5 and operating lever 6.
  • the body has a longitudinal gap 7. At the lower body end the gap is open only to one side of the body, but at the upper end the gap extends through the body forming a sliding slot for the rod of piston 3. At the lower body end hole 8 forms an extension to the gap. At the upper end an end stop 9 closes the gap.
  • the cylinder and piston 3 are placed in gap 7 so that the cylinder head extends out of hole 8 at the lower body end.
  • a peak-like flange 10 which at the mid-point of the body is pushed into a transverse groove 11 opening to the open side of the gap. In this way the cylinder is prevented from moving in a longitudinal direction.
  • the piston has a rod emerging at the upper cylinder end which at its end in a sliding slot has a pulling knob 12 with edges extending from the gap to the sides of the body. Liquid is drawn into the cylinder by pulling the piston upward at the knob. Stop 9 at the top end of the sliding slot prevents the piston from being pulled out of the cylinder by mistake.
  • the figures show the piston both in its top and lower positions.
  • the top end of the striker 4 has gripping means 5 running in a sliding slot, which co-operates with the rod of piston 3 and which when moving downward will grip the piston rod and move it downward, but which when moving upward is free of the piston rod, thus not moving the piston.
  • the rod of piston 3 is provided on the striker side with teeth 13 and the gripping means 5 is a sharp cam pressing obliquely from above into these teeth.
  • the gripping means is made to operate more reliably, but control can only be arranged stepwise.
  • the teeth are made easily at the same time as the piston.
  • the striker is connected together with a spring 14, which forces the striker upward against end 21 in the sliding slot.
  • This stop end is chamfered at the top and thus it lifts gripping means 5 off the rod of piston 3 when the gripping means is forced to its top position.
  • spring 14 is a draw-spring having one end attached to the striker 4 and the other end to a control rod 20 located at the top body end.
  • the top body end has a control rod 20 movable lengthwise of the body. Its lower end 21 works as a cam to lift the top end 22 of the striker off the teeth 13 when the striker is in its top position. Gripping means 5 is located on the striker at an interval from its top end. Thus, the position of the control rod determines at which point the gripping means will engage with the teeth.
  • a transverse pin 23 is fitted to move in a cam groove rotating eccentrically in control disc 24. The rod position can thus be controlled - by rotating the disc.
  • the body and control disc are provided with a scale from which the volume corresponding to a dosing stroke can be read.
  • Plate 16 covers spring 14 and the control rod 20.
  • Figures 1 and 2 also show the shape of piston 3 which corresponds to the cylinder 2 shape narrowing conically from below sealing point 25. Groove 7 here extends all the way to the lower body end.
  • the pipette according to the invention is gripped like a pencil and operating lever 6 is pushed with the forefinger.
  • the pipette can thus be brought very precisely even to a small object. Dosing too is easy.
  • the pipette is especially suitable for use, for example, in certain immunofluorescence determinations performed with glass plates, where liquid must be dosed onto very small areas.
  • the hand When dosing, the hand may also be supported against the table, which is not possible when using conventional pipettes.
  • the pipette is also very suitable for glueing requiring high precision, for example, in assembly jobs.
  • glue is usually dosed from a compressable bottle in such jobs.
  • Using a pipette according to the invention is much easier and the doses ore essentially more exact. Nor will the glue dry in the cylinder as easily as in a bottle.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Centrifugal Separators (AREA)

Claims (13)

  1. Pipette à piston comprenant un corps longitudinal (1) muni d'un espace cylindrique, servant de poignée et ayant une extrémité supérieure et une extrémité inférieure, un cylindre (2) s'étendant dans l'espace cylindrique et ayant une extrémité inférieure pointue sortant de l'extrémité de corps inférieure et munie d'une ouverture destinée aux liquides ; un piston (3) situé dans ledit cylindre (2) ; un moyen de traction (12) permettant de déplacer le piston (3) vers le haut dans le cylindre (2), un moyen de transfert (4) et un moyen de saisie (5) fixé sur celui-ci permettant de déplacer le piston (3) vers le bas dans le cylindre (2), caractérisée en ce que ledit piston (3) présente une tige de piston qui sort au niveau de l'extrémité supérieure dudit espace cylindrique, en ce que ladite pipette comprend en outre une tige de commande (20) permettant de déterminer la position d'engagement dudit élément de saisie (5) avec ladite tige de piston ; et en ce qu'un ressort (14) est fixé audit moyen de transfert (4) et à ladite tige de commande (20), lequel ressort (14) est agencé de façon à tirer le moyen de transfert (4) avec le moyen de saisie (5) à la fois en direction de l'extrémité supérieure du corps (1) et vers ladite tige de piston à mesure que le moyen de transfert (4) est déplacé vers l'extrémité inférieure de la pipette.
  2. Pipette selon la revendication 1, caractérisée en ce que la tige de piston présente des dents (13) et en ce que le moyen de saisie (5) est une saillie coopérant avec lesdites dents (13).
  3. Pipette selon la revendication 1 ou 2, caractérisée en ce qu'elle comprend un butoir (21 ; 21') pour décoller le moyen de saisie (5) de la tige de piston dans la position supérieure du moyen de saisie (5), ce qui permet au piston (3) d'être tiré librement jusqu'à sa position supérieure.
  4. Pipette selon l'une des revendications 1 à 3, caractérisée en ce qu'un mouvement du moyen de saisie (5) vers le bas pousse le piston vers le bas (3) uniquement sur une partie de la distance totale disponible du piston (3).
  5. Pipette selon la revendication 4, caractérisée en ce que le corps (1) présente une butée (9) pour limiter la position supérieure de la course du piston (3).
  6. Pipette selon l'une des revendications 1 à 5, caractérisée en ce qu'au moins le cylindre (2) ou le piston (3) est échangeable.
  7. Pipette selon la revendication 6, caractérisée en ce qu'il y a un interstice longitudinal (7) sur le côté du corps de pipette (1).
  8. Pipette selon la revendication 7, caractérisée en ce que ledit interstice (7) se trouve du côté du corps opposé au moyen de transfert (4).
  9. Pipette selon la revendication 7 ou 8, caractérisée en ce que l'extrémité supérieure dudit cylindre (2) présente une saillie (10) et en ce que le corps (1) présente une rainure transversale correspondante (11) dans laquelle est insérée ladite saillie (10).
  10. Pipette selon l'une des revendications 1 à 10, caractérisée en ce que l'extrémité de tige de piston est munie d'un bouton de traction (12).
  11. Utilisation d'une pipette selon l'une des revendications 1 à 10 pour doser un liquide.
  12. Utilisation selon la revendication 11 pour des déterminations d'immunofluorescence.
  13. Utilisation, selon la revendication 11, dans laquelle ledit liquide est de la colle.
EP94911982A 1993-04-21 1994-04-20 Pipette a piston Expired - Lifetime EP0695220B1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FI931806A FI931806A0 (fi) 1993-04-21 1993-04-21 Kolvpipett
FI931806 1993-04-21
PCT/FI1994/000149 WO1994023838A1 (fr) 1993-04-21 1994-04-20 Pipette a piston

Publications (2)

Publication Number Publication Date
EP0695220A1 EP0695220A1 (fr) 1996-02-07
EP0695220B1 true EP0695220B1 (fr) 1998-09-02

Family

ID=8537784

Family Applications (1)

Application Number Title Priority Date Filing Date
EP94911982A Expired - Lifetime EP0695220B1 (fr) 1993-04-21 1994-04-20 Pipette a piston

Country Status (6)

Country Link
EP (1) EP0695220B1 (fr)
JP (1) JP3697527B2 (fr)
AU (1) AU6431894A (fr)
DE (1) DE69413021T2 (fr)
FI (1) FI931806A0 (fr)
WO (1) WO1994023838A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100624491B1 (ko) 2004-10-20 2006-09-15 코아스템(주) 연필형 피펫
JP6353701B2 (ja) * 2014-05-22 2018-07-04 株式会社アイカムス・ラボ 分注装置

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3975960A (en) * 1974-03-15 1976-08-24 Technicon Instruments Corporation Manual fluid sampler with overstroke
US4099548A (en) * 1976-08-25 1978-07-11 Oxford Laboratories Inc. Hand-held pipette for repetitively dispensing precise volumes of liquid
FI831239A0 (fi) * 1983-04-13 1983-04-13 Labsystems Oy Doseringspipett

Also Published As

Publication number Publication date
AU6431894A (en) 1994-11-08
DE69413021D1 (de) 1998-10-08
FI931806A0 (fi) 1993-04-21
DE69413021T2 (de) 1999-02-18
JPH08510158A (ja) 1996-10-29
EP0695220A1 (fr) 1996-02-07
JP3697527B2 (ja) 2005-09-21
WO1994023838A1 (fr) 1994-10-27

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