EP0682267A1 - Nmr chiral shift reagent comprising sugar derivative - Google Patents

Nmr chiral shift reagent comprising sugar derivative Download PDF

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Publication number
EP0682267A1
EP0682267A1 EP95901598A EP95901598A EP0682267A1 EP 0682267 A1 EP0682267 A1 EP 0682267A1 EP 95901598 A EP95901598 A EP 95901598A EP 95901598 A EP95901598 A EP 95901598A EP 0682267 A1 EP0682267 A1 EP 0682267A1
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Prior art keywords
nmr
shift reagent
chiral shift
group
saccharide
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German (de)
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EP0682267A4 (en
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Yoshio Okamoto
Eiji Yashima
Kazuma Oguni
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Daicel Corp
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Daicel Chemical Industries Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00Preparations for testing in vivo
    • A61K49/06Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01RMEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00Arrangements or instruments for measuring magnetic variables
    • G01R33/20Arrangements or instruments for measuring magnetic variables involving magnetic resonance
    • G01R33/28Details of apparatus provided for in groups G01R33/44 - G01R33/64
    • G01R33/281Means for the use of in vitro contrast agents
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/24Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

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  • the present invention relates to an optically active shift reagent (chiral shift reagent) used for analyzing an enantiometric mixture by separating signals assignable to the enantiomers of a compound having an asymmetric carbon atom in nuclear magnetic resonance spectrometry (hereinafter referred to as NMR).
  • NMR nuclear magnetic resonance spectrometry
  • the present invention relates to a chiral shift reagent for NMR comprising a saccharide derivative, and a process for analyzing the mixing ratio of optical isomers in an optical isomer mixture or the optical purity and absolute configuration of an optically active substance with the chiral shift reagent.
  • optically active compounds are generally useful as pharmaceuticals, agricultural chemicals and flavors, or starting materials of them, the effect of one isomer on a living body is usually different from that of the other. Therefore, it has become very important subject to separate and quantify the optical isomers for the improvement of the medicinal effect per unit dose and for the prevention of side effects and chemical injury.
  • lanthanide chiral shift reagents are well known as the one to be used in the method for analyzing optically active substances by NMR (H. L. Goering et al., J. Am. Chem. Soc., 93 , 5913(1971); D. Valentine et al., J. Org. Chem., 41 , 62(1976); P. Reisberg et al., J. Pharm. Sci., 65 , 592(1976); J. A. Kroll, J.
  • W. H. Pirkle et al. have used an optically active aromatic amine as a chiral solvent (W. H. Pirkle, J. Am. Chem. Soc., 88 , 1837(1966); T. G. Burlingame et al., J. Am. Chem. Soc., 88 , 4294(1966); W. H. Pirkle et al., Tetrahedron Letters, 2579(1968); and others), and have analyzed optically active compounds .
  • Toda et al. have disclosed a method for analyzing optically active substances with an optically active biphenyl derivative as the chiral shift reagent in Japanese Patent Publication-A No. 4-193842, this method is employable in only a limited range of application and it is difficult to apply it for generalized use.
  • the present invention provides a chiral shift reagent for NMR comprising a saccharide derivative and an analytical method characterized by measuring an NMR spectrum of an optical isomer mixture or an optically active substance in the presence of such a chiral shift reagent to analyze the mixing ratio of the optical isomers or the optical purity and absolute configuration of the optically active substance on the basis of the spectrum.
  • the method for analyzing the mixing ratio of optical isomers in an optical isomer mixture or the optical purity and absolute configuration of an optically active substance characterized by measuring an NMR spectrum of the optical isomer mixture or the optically active substance in the presence of a chiral shift reagent for NMR comprising a saccharide derivative to analyze the mixing ratio of the optical isomers or the optical purity and absolute configuration of the optically active substance on the basis of the spectrum.
  • the saccharide of the saccharide derivative is preferably a monosaccharide, an oligosaccharide or a poly-saccharide.
  • the saccharide derivative is preferably one obtained by substituting at least one group in those consisting of an alkyl group, an aryl group, a nitro group, an alkenyl group, an acyl group and a carbamoyl group for a part or all of the hydrogen atom(s) of the hydroxyl groups or amino groups of a saccharide. Further, the present invention is a utilization of a saccharide derivative for the above-described use.
  • the saccharide of the saccharide derivative to be used in the present invention may be any of monosaccharides, oligosaccharides and polysaccharides, so far as it is optically active. Further, as for the chemical structure of the monosaccharide, the compound may have a ring in its molecule or not. Furthermore, as for the oligosaccharide and polysaccharide, the compound may be linear or cyclic one.
  • allose, altrose, galactose, glucose, mannose, talose, xylose and the like are cited for monosaccharides; sucrose, lactose, maltose, cellobiose, isomaltose, chitobiose, mannobiose, xylobiose, cyclodextrin, cellooligosaccharides (degree of polymerization of glucose 2 to 8), maltooligosaccharides (degree of polymerization of glucose 2 to 8) and the like for oligosaccharides; and ⁇ -1,4-glucan (cellulose), ⁇ -1,4-glucans (amylose and amylopectin), ⁇ -1,6-glucan (dextran), ⁇ -1,6-glucan, ⁇ -1,3-glucan, ⁇ -1,3-glucan, ⁇ -1,2-glucan, ⁇ -1,4-galactan, ⁇ -1,4-mannan
  • the saccharide derivatives to be used in the present invention include ones obtained by substituting an alkyl group, an aryl group, a nitro group, an alkenyl group, an acyl group, a carbamoyl group or the like for a part or all of the hydrogen atom(s) of the hydroxyl groups or amino groups of the saccharides exemplified above.
  • Particular examples of the atomic groups which are introduced into the saccharides include compounds which form, by reacting them with the hydroxyl group or amino group of the saccharide, an ester bond, an urethane bond, an ether bond, an amide bond, an urea bond, an imino bond and the like which are represented by the following formulae (1) to (7).
  • R represents a group of which constituent carbon atom number(s) is(are) 1 to 30, which may have an unsaturated bond.
  • R may represent a phenyl group represented by the following formula (8).
  • R1 through R5 each represents a hydrogen atom, an alkyl or alkylsilyl group having 1 to 5 carbon atoms, a halogen atom or a nitro group.
  • the degree of substitution for all the hydrogen atoms in the hydroxyl groups and amino groups of the saccharide derivative of the present invention is preferably 40 to 100%, still more preferably 80% or above.
  • the saccharides are easily available, since they occur in a large amount in nature.
  • the ester or amide derivatives of the saccharides can be easily synthesized by reacting the saccharide with a carboxylic acid chloride corresponding to the group represented by the above formulae (1) or (5) in a suitable solvent at a high temperature.
  • the carbamate or urea derivatives of the saccharides can be obtained by reacting the saccharide with an isocyanate corresponding to the group represented by the above formulae (2) or (6) in a suitable solvent.
  • the imine or ether derivatives of the saccharides can be easily synthesized by reacting the saccharide with a halide corresponding to the group represented by the above formulae (3) or (7) under heating in a suitable solvent.
  • nitric esters of the saccharides can also be easily obtained by reacting the saccharide with nitric acid at a high temperature.
  • the chiral shift reagent of the present invention comprising a saccharide derivative
  • the compound to be analyzed is dissolved in a suitable solvent and a suitable amount of the chiral shift reagent of the present invention is added thereto, followed by the measurement of the NMR spectrum.
  • the NMR spectrum of the compound, which is the object of the analysis is measured in such a manner, the one peak, which is observed when the chiral shift reagent of the present invention is not added, is split into two peaks in many compounds.
  • Each of the two peaks to be observed in the NMR corresponds to each of the optical isomers, and the optical purity can be calculated based on the integrated intensity thereof. Further, it is also possible to empirically relate the positions of the peaks to the structures (the absolute configurations) of the optical isomers.
  • the chiral shift reagent of the present invention is usable for both 1H-NMR and 13C-NMR.
  • Fig. 1 is a drawing of the 13C-NMR spectra measured in the presence of the chiral shift reagent in Example 1.
  • Fig. 2 is a drawing of the 13C-NMR spectra measured in the presence of the chiral shift reagent in Example 2.
  • Fig. 3 is a drawing of the 13C-NMR spectra measured in the presence of the chiral shift reagent in Example 3.
  • Fig. 4 is a drawing of the 1H-NMR spectrum measured in the presence of the chiral shift reagent in Example 4.
  • Fig. 5 is a drawing of the 1H-NMR spectrum measured in the presence of the chiral shift reagent in Example 5.
  • Fig. 1 is a drawing of the 13C-NMR spectra measured in the presence of the chiral shift reagent in Example 1.
  • Fig. 2 is a drawing of the 13C-NMR spectra measured in the presence of the chiral shift reagent in Example 2.
  • Fig. 3 is
  • FIG. 6 is a drawing of the 1H-NMR spectrum measured in the presence of the chiral shift reagent in Example 6.
  • Fig. 7 is a drawing of the 1H-NMR spectrum measured in the presence of the chiral shift reagent in Example 7.
  • Fig. 8 is a drawing of the 1H-NMR spectrum measured in the presence of the chiral shift reagent in Example 8.
  • racemic 1-indanol represented by the following formula (9) was mixed with 100 mg of cellulose tris(p-methylbenzoate) having glucopyranose units equimolar to the 1-indanol (hereinafter abbreviated to chiral shift reagent (1)).
  • the mixture was dissolved in about 1.0 g of chloroform-d.
  • the chemical shift given in Table 1 was obtained.
  • the chemical shift of 1-indanol as measured in the absence of the chiral shift reagent (1) at -35°C is given in the brackets.
  • Optical isomers of 1-phenylethanol (R isomer and S isomer) represented by the following formula (10) were mixed with the chiral shift reagent (1) in a molar ratio of 2 : 1 : 1 (48 mg : 24 mg : 100 mg by weight ratio). About 1.0 g of chloroform-d was added thereto. As a result of the 13C-NMR measurement at 35°C, similar to Example 1, the chemical shift given in Table 2 was obtained. The assignments of R isomer and S isomer of 1-phenylethanol in the respective peaks are shown. For comparison, the chemical shift of 1-phenylethanol as measured in the absence of the chiral shift reagent (1) at 35°C is given in the brackets.

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Abstract

A chiral shift reagent comprising a sugar derivative for ¹H-NMR and ¹³C-NMR spectrometry, of which the starting material is readily available and the synthesis is easy and which has good performance. An analytical method which comprises measuring the NMR spectra of an optical isomer mixture or an optically active compound in the presence of the chiral shift reagent and determining the mixing ratio of the above mixture or the optical purity and absolute configuration of the above compound.

Description

    Field of Industrial Application
  • The present invention relates to an optically active shift reagent (chiral shift reagent) used for analyzing an enantiometric mixture by separating signals assignable to the enantiomers of a compound having an asymmetric carbon atom in nuclear magnetic resonance spectrometry (hereinafter referred to as NMR). In particular, the present invention relates to a chiral shift reagent for NMR comprising a saccharide derivative, and a process for analyzing the mixing ratio of optical isomers in an optical isomer mixture or the optical purity and absolute configuration of an optically active substance with the chiral shift reagent.
  • Prior Art
  • Although optically active compounds are generally useful as pharmaceuticals, agricultural chemicals and flavors, or starting materials of them, the effect of one isomer on a living body is usually different from that of the other. Therefore, it has become very important subject to separate and quantify the optical isomers for the improvement of the medicinal effect per unit dose and for the prevention of side effects and chemical injury.
  • Although lanthanide chiral shift reagents are well known as the one to be used in the method for analyzing optically active substances by NMR (H. L. Goering et al., J. Am. Chem. Soc., 93, 5913(1971); D. Valentine et al., J. Org. Chem., 41, 62(1976); P. Reisberg et al., J. Pharm. Sci., 65, 592(1976); J. A. Kroll, J. Forenesic Sci., 24, 303(1976); and others), these shift reagents have the disadvantage of incapability of generalized use, because it is difficult to synthesize or handle them, they are expensive and apt to be decomposed in the presence of moisture or the like, and it is difficult to handle them.
  • On the other hand, in the analysis of optically active compounds, W. H. Pirkle et al. have used an optically active aromatic amine as a chiral solvent (W. H. Pirkle, J. Am. Chem. Soc., 88, 1837(1966); T. G. Burlingame et al., J. Am. Chem. Soc., 88, 4294(1966); W. H. Pirkle et al., Tetrahedron Letters, 2579(1968); and others), and have analyzed optically active compounds . Further, similarly, one example wherein amines and ester derivatives of amino acids are analyzed with optically active 2,2,2-trifluorophenylethanol as a chiral solvent, and the like have been known (W. H. Pirkle et al., Tetrahedron Letters, 5489(1968); W. H. Pirkle et al., J. Am. Chem, Soc., 91, 5150(1969); and others). However, these chiral solvents usually have such a defect that their abilities of separating the signals assignable to the optical isomers is poor.
  • While, although Toda et al. have disclosed a method for analyzing optically active substances with an optically active biphenyl derivative as the chiral shift reagent in Japanese Patent Publication-A No. 4-193842, this method is employable in only a limited range of application and it is difficult to apply it for generalized use.
  • The above-described chiral shift reagents were each developed as a reagent for analyzing optical isomers by shifting the resonance signals in ¹H-NMR, whereas only a few reports were made on chiral shift reagents for ¹³C-NMR. For example, although R. R. Fraser et al. have reported tris[3-heptafluoropropylhydroxymethylene-d-camphorato]praseodymium or tris[3-heptafluoropropylhydroxymethylene-d-camphorato]europium which is a lanthanide chiral shift reagent, similar to those for ¹H-NMR (R. R. Fraser et al., J. Magn. Resonance, 10, 95-97(1973)), it is difficult to use these compounds because of the hygroscopicity thereof, like those described above.
  • Disclosure of the Present Invention
  • Under these circumstances, the present inventors have made extensive studies for the development of a chiral shift reagent for ¹H-NMR and ¹³C-NMR, of which starting materials are easily available, of which synthesis is easy and of which performance is excellent. As a result, they have found that saccharide derivatives exhibit the effects as chiral shift reagents, thus completing the present invention.
  • Thus, the present invention provides a chiral shift reagent for NMR comprising a saccharide derivative and an analytical method characterized by measuring an NMR spectrum of an optical isomer mixture or an optically active substance in the presence of such a chiral shift reagent to analyze the mixing ratio of the optical isomers or the optical purity and absolute configuration of the optically active substance on the basis of the spectrum. More specifically, it is a method for analyzing the mixing ratio of optical isomers in an optical isomer mixture or the optical purity and absolute configuration of an optically active substance, characterized by measuring an NMR spectrum of the optical isomer mixture or the optically active substance in the presence of a chiral shift reagent for NMR comprising a saccharide derivative to analyze the mixing ratio of the optical isomers or the optical purity and absolute configuration of the optically active substance on the basis of the spectrum. The saccharide of the saccharide derivative is preferably a monosaccharide, an oligosaccharide or a poly-saccharide. The saccharide derivative is preferably one obtained by substituting at least one group in those consisting of an alkyl group, an aryl group, a nitro group, an alkenyl group, an acyl group and a carbamoyl group for a part or all of the hydrogen atom(s) of the hydroxyl groups or amino groups of a saccharide. Further, the present invention is a utilization of a saccharide derivative for the above-described use.
  • A detailed description will now be made on the present invention.
  • 〈Saccharides〉
  • The saccharide of the saccharide derivative to be used in the present invention may be any of monosaccharides, oligosaccharides and polysaccharides, so far as it is optically active. Further, as for the chemical structure of the monosaccharide, the compound may have a ring in its molecule or not. Furthermore, as for the oligosaccharide and polysaccharide, the compound may be linear or cyclic one. As examples of these saccharides, allose, altrose, galactose, glucose, mannose, talose, xylose and the like are cited for monosaccharides; sucrose, lactose, maltose, cellobiose, isomaltose, chitobiose, mannobiose, xylobiose, cyclodextrin, cellooligosaccharides (degree of polymerization of glucose 2 to 8), maltooligosaccharides (degree of polymerization of glucose 2 to 8) and the like for oligosaccharides; and β-1,4-glucan (cellulose), α-1,4-glucans (amylose and amylopectin), α-1,6-glucan (dextran), β-1,6-glucan, β-1,3-glucan, α-1,3-glucan, β-1,2-glucan, β-1,4-galactan, β-1,4-mannan, α-1,6-mannan, β-1,2-fructan, β-2,6-fructan, β-1,6-glucan, β-1,3-glucan, α-1,3-glucan, β-1,2-glucan, β-1,4-galactan, β-1,4-xylan, β-1,3-xylan, β-1,4-chitosan, β-1,4-acetylchitosan (chitin), pullulan, agarose, alginic acid and the like for polysaccharides. The upper limit of the average degree of polymerization of these polysaccharides is 1000, and it is preferred that the average degree of polymerization thereof be 500 or below for the measurement of the NMR spectrum.
  • 〈Saccharide derivatives〉
  • The saccharide derivatives to be used in the present invention include ones obtained by substituting an alkyl group, an aryl group, a nitro group, an alkenyl group, an acyl group, a carbamoyl group or the like for a part or all of the hydrogen atom(s) of the hydroxyl groups or amino groups of the saccharides exemplified above. Particular examples of the atomic groups which are introduced into the saccharides include compounds which form, by reacting them with the hydroxyl group or amino group of the saccharide, an ester bond, an urethane bond, an ether bond, an amide bond, an urea bond, an imino bond and the like which are represented by the following formulae (1) to (7).
    Figure imgb0001

       In the above formulae (1) to (7), R represents a group of which constituent carbon atom number(s) is(are) 1 to 30, which may have an unsaturated bond. Alternatively, R may represent a phenyl group represented by the following formula (8).
    Figure imgb0002

       In the above formula (8), R₁ through R₅ each represents a hydrogen atom, an alkyl or alkylsilyl group having 1 to 5 carbon atoms, a halogen atom or a nitro group.
  • The degree of substitution for all the hydrogen atoms in the hydroxyl groups and amino groups of the saccharide derivative of the present invention is preferably 40 to 100%, still more preferably 80% or above.
  • The saccharides are easily available, since they occur in a large amount in nature. The ester or amide derivatives of the saccharides can be easily synthesized by reacting the saccharide with a carboxylic acid chloride corresponding to the group represented by the above formulae (1) or (5) in a suitable solvent at a high temperature. The carbamate or urea derivatives of the saccharides can be obtained by reacting the saccharide with an isocyanate corresponding to the group represented by the above formulae (2) or (6) in a suitable solvent. The imine or ether derivatives of the saccharides can be easily synthesized by reacting the saccharide with a halide corresponding to the group represented by the above formulae (3) or (7) under heating in a suitable solvent. Further, nitric esters of the saccharides can also be easily obtained by reacting the saccharide with nitric acid at a high temperature.
  • 〈Method for use as chiral shift reagent〉
  • When the chiral shift reagent of the present invention comprising a saccharide derivative is used in the method for analyzing the mixing ratio of optical isomers in an optical isomer mixture or the optical purity and absolute configuration of an optically active substance, the compound to be analyzed is dissolved in a suitable solvent and a suitable amount of the chiral shift reagent of the present invention is added thereto, followed by the measurement of the NMR spectrum. When the NMR spectrum of the compound, which is the object of the analysis, is measured in such a manner, the one peak, which is observed when the chiral shift reagent of the present invention is not added, is split into two peaks in many compounds. Each of the two peaks to be observed in the NMR corresponds to each of the optical isomers, and the optical purity can be calculated based on the integrated intensity thereof. Further, it is also possible to empirically relate the positions of the peaks to the structures (the absolute configurations) of the optical isomers.
  • The chiral shift reagent of the present invention is usable for both ¹H-NMR and ¹³C-NMR.
  • Brief Description of the Drawings
  • Fig. 1 is a drawing of the ¹³C-NMR spectra measured in the presence of the chiral shift reagent in Example 1. Fig. 2 is a drawing of the ¹³C-NMR spectra measured in the presence of the chiral shift reagent in Example 2. Fig. 3 is a drawing of the ¹³C-NMR spectra measured in the presence of the chiral shift reagent in Example 3. Fig. 4 is a drawing of the ¹H-NMR spectrum measured in the presence of the chiral shift reagent in Example 4. Fig. 5 is a drawing of the ¹H-NMR spectrum measured in the presence of the chiral shift reagent in Example 5. Fig. 6 is a drawing of the ¹H-NMR spectrum measured in the presence of the chiral shift reagent in Example 6. Fig. 7 is a drawing of the ¹H-NMR spectrum measured in the presence of the chiral shift reagent in Example 7. Fig. 8 is a drawing of the ¹H-NMR spectrum measured in the presence of the chiral shift reagent in Example 8.
  • Examples
  • The present invention will be illustrated hereinafter by referring to Examples in greater detail. However, it is needless to say that the present invention is not limited to them.
  • Example 1
  • 26 mg of racemic 1-indanol represented by the following formula (9) was mixed with 100 mg of cellulose tris(p-methylbenzoate) having glucopyranose units equimolar to the 1-indanol (hereinafter abbreviated to chiral shift reagent (1)). The mixture was dissolved in about 1.0 g of chloroform-d. As a result of the measurement (with JEOL JNM A-500) of the ¹³C-NMR spectra at -35°C, the chemical shift given in Table 1 was obtained. For comparison, the chemical shift of 1-indanol as measured in the absence of the chiral shift reagent (1) at -35°C is given in the brackets.
    Figure imgb0003
    Table 1
    Position of carbon atom Chemical shift (ppm) Position of carbon atom Chemical shift (ppm)
    1 76.15 [76.18 ] 6 128.12 [128.15] 128.15
    2 35.77 [35.72 ] 35.81 7 126.85 [126.58]
    3 29.64 [29.74 ] 8 124.10 [124.24]
    4 143.22 [143.23] 143.24 9 144.85 [145.03] 144.88
    5 124.85 [124.77]
  • The actual splittings of the ¹³C-NMR spectra are shown in Fig. 1.
  • Example 2
  • Optical isomers of 1-phenylethanol (R isomer and S isomer) represented by the following formula (10) were mixed with the chiral shift reagent (1) in a molar ratio of 2 : 1 : 1 (48 mg : 24 mg : 100 mg by weight ratio). About 1.0 g of chloroform-d was added thereto. As a result of the ¹³C-NMR measurement at 35°C, similar to Example 1, the chemical shift given in Table 2 was obtained. The assignments of R isomer and S isomer of 1-phenylethanol in the respective peaks are shown. For comparison, the chemical shift of 1-phenylethanol as measured in the absence of the chiral shift reagent (1) at 35°C is given in the brackets.
    Figure imgb0004
    Table 2
    Position of carbon atom Chemical shift (ppm)
    1 70.214 (R) [70.11 ] 70.224(S)
    2 25.178 (S) [25.06 ] 25.184 (R)
    1' 145.990 (S) [145.92] 146.009 (R)
    2', 6' 125.436 [125.42]
    3', 5' 128.441 (R) [128.36] 128.444 (S)
    4' 127.337 (R) [127.26] 127.348 (S)
  • The actual splittings of the ¹³C-NMR spectra are shown in Fig. 2.
  • Example 3
  • 30 mg of racemic glutethimide represented by the following formula (11) was mixed with 100 mg of the chiral shift reagent (1). About 1.0 g of chloroform-d was added thereto. As a result of the ¹³C-NMR measurement at 35°C, similar to Example 1, the chemical shift given in Table 3 was obtained. For comparison, the chemical shift of glutethimide as measured in the absence of the chiral shift reagent (1) at 35°C is given in the brackets.
    Figure imgb0005
    Table 3
    Position of carbon atom Chemical shift (ppm)
    2 175.05 [175.39] 175.07
    3 51.22 [51.14 ]
    4 29.33 [29.32 ]
    5 27.19 [27.19 ] 27.22
    6 172.12 [172.73] 172.16
    7 32.89 [32.90 ] 32.91
    8 9.11 [9.06]
    9 138.89 [138.92] 138.95
    10, 14 126.18 [126.21]
    11, 13 129.03 [129.00]
    12 127.60 [127.56] 127.61
  • The actual splittings of the ¹³C-NMR spectra are shown in Fig. 3.
  • Example 4
  • 20 mg of cellulose tris(3,5-dichlorophenylcarbamate) (hereinafter abbreviated to chiral shift reagent (2)) was added to 5.6 mg of racemic trans-stilbene oxide represented by the following formula (12), and about 8 mg of isopropyl alcohol was further added thereto, followed by dissolving them in about 1.0 ml (1.5 g) of chloroform-d. As a result of the ¹H-NMR measurement at 22°C, the chemical shift given in Table 4 was obtained. For comparison, the chemical shift of trans-stilbene oxide as measured in the absence of the chiral shift reagent (2) at 22°C is given in the brackets.
    Figure imgb0006
    Table 4
    Position of hydrogen atom Chemical shift (ppm)
    1 3.874 [3.870] 3.877
  • The actual ¹H-NMR spectrum is shown in Fig. 4.
  • Example 5
  • 21 mg of tris(3,5-dimethylphenylcarbamoyl) maltooligosaccharide having a degree of polymerization of 6 (hereinafter abbreviated to chiral shift reagent (3)) was added to 5.0 mg of racemic trans-stilbene oxide represented by the above formula (12), followed by dissolving them in about 0.9 ml (1.35 g) of chloroform-d. As a result of the ¹H-NMR measurement at 22°C, the chemical shift given in Table 5 was obtained. For comparison, the chemical shift of trans-stilbene oxide as measured in the absence of the chiral shift reagent (3) at 22°C is given in the brackets. Table 5
    Position of hydrogen atom Chemical shift (ppm)
    1 3.872 [3.870 ] 3.880
  • The actual ¹H-NMR spectrum is shown in Fig. 5.
  • Example 6
  • 21 mg of tris(3,5-dimethylphenylcarbamoyl) maltooligosaccharide having a degree of polymerization of 5 (hereinafter abbreviated to chiral shift reagent (4)) was added to 5.0 mg of racemic trans-stilbene oxide representd by the above formula (12), followed by dissolving them in about 0.9 ml (1.35 g) of chloroform-d. As a result of the ¹H-NMR measurement at 22°C, the chemical shift given in Table 6 was obtained. For comparison, the chemical shift of trans-stilbene oxide as measured in the absence of the chiral shift reagent (4) at 22°C is given in the brackets. Table 6
    Position of hydrogen atom Chemical shift (ppm)
    1 3.871 [3.870 ] 3.878
  • The actual ¹H-NMR spectrum is shown in Fig. 6.
  • Example 7
  • 21 mg of tris(3,5-dimethylphenylcarbamoyl) maltooligosaccharide having a degree of polymerization of 3 (hereinafter abbreviated to chiral shift reagent (5)) was added to 5.0 mg of racemic trans-stilbene oxide represented by the above formula (12), followed by dissolving them in about 0.9 ml (1.35 g) of chloroform-d. As a result of the ¹H-NMR measurement at 22°C, the chemical shift given in Table 7 was obtained. For comparison, the chemical shift of trans-stilbene oxide as measured in the absence of the chiral shift reagent (5) at 22°C is given in the brackets. Table 7
    Position of hydrogen atom Chemical shift (ppm)
    1 3.871 [3.870 ] 3.874
  • The actual ¹H-NMR spectrum is shown in Fig. 7.
  • Example 8
  • 20 mg of cellulose tris(4-trimethylsilylphenylcarbamate) represented by the following formula (13) (hereinafter abbreviated to chiral shift reagent (6)) was added to 5.0 mg of racemic trans-stilbene oxide represented by the above formula (12), followed by dissolving them in about 1.0 ml (1.5 g) of chloroform-d. As a result of the ¹H-NMR measurement at 22°C, the chemical shift given in Table 8 was obtained. For comparison, the chemical shift of trans-stilbene oxide as measured in the absence of the chiral shift reagent (6) at 22°C is given in the brackets.
    Figure imgb0007
    Table 8
    Position of hydrogen atom Chemical shift (ppm)
    1 3.870 [3.871 ] 3.872
  • The actual ¹H-NMR spectrum is shown in Fig. 8.

Claims (3)

  1. A method for analyzing the mixing ratio of optical isomers in an optical isomer mixture or the optical purity and absolute configuration of an optically active substance, characterized by measuring an NMR spectrum of the optical isomer mixture or the optically active substance in the presence of a chiral shift reagent for NMR comprising a saccharide derivative to analyze the mixing ratio of the optical isomers or the optical purity and absolute configuration of the optically active substance on the basis of the spectrum.
  2. The method according to Claim 1, wherein the saccharide of the saccharide derivative is a monosaccharide, an oligosaccharide or a polysaccharide.
  3. The method according to Claim 1, wherein the saccharide derivative is one obtained by substituting at least one group in those consisting of an alkyl group, an aryl group, a nitro group, an alkenyl group, an acyl group and a carbamoyl group for a part or all of the hydrogen atom(s) of the hydroxyl groups or amino groups of a saccharide.
EP95901598A 1993-11-26 1994-11-25 Nmr chiral shift reagent comprising sugar derivative. Withdrawn EP0682267A4 (en)

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JP296318/93 1993-11-26
JP29631893 1993-11-26
JP6280153A JPH07198818A (en) 1993-11-26 1994-11-15 Chiral shift reagent for nmr, composed of saccharide derivative
JP280153/94 1994-11-15
PCT/JP1994/001992 WO1995014934A1 (en) 1993-11-26 1994-11-25 Nmr chiral shift reagent comprising sugar derivative

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WO2008088830A2 (en) * 2007-01-16 2008-07-24 Evolved Nanomaterial Sciences, Inc. Chiral separating agents with active support
JP6478188B2 (en) * 2015-06-09 2019-03-06 国立研究開発法人物質・材料研究機構 NMR chiral shifter and optical purity determination method using the same
CN107505347A (en) * 2017-07-12 2017-12-22 广西师范大学 A kind of abietyl phosphine derivatization pre-treating method for NMR Chiral detection

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3915641A (en) * 1971-11-03 1975-10-28 Wisconsin Alumni Res Found NMR method for determination of enantiomeric compositions with chiral shift reagents
WO1991004968A1 (en) * 1989-10-06 1991-04-18 University Of South Carolina Chalcogen-based chiral reagents and method for nuclear magnetic resonance detection of stereochemical assignments and enantiomeric ratios and method for synthesizing said reagents

Family Cites Families (7)

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Publication number Priority date Publication date Assignee Title
JPS58153165A (en) * 1982-12-29 1983-09-12 Daikin Ind Ltd Chiral analytical reagent
JPS58153164A (en) * 1982-12-29 1983-09-12 Daikin Ind Ltd Chiral analytical reagent
JPS59212744A (en) * 1983-05-19 1984-12-01 Sumitomo Chem Co Ltd Shift reagent for nmr
JPS63186134A (en) * 1987-01-29 1988-08-01 Ube Ind Ltd Chiral analysis reagent
JPH01301156A (en) * 1988-02-02 1989-12-05 Nippon Soda Co Ltd Chiral shift reagent
JPH02259481A (en) * 1989-03-31 1990-10-22 Nippon Soda Co Ltd Chirality shift reagent
JP2872800B2 (en) * 1990-10-29 1999-03-24 ダイセル化学工業株式会社 Optically active biphenyl derivative and optical resolution method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3915641A (en) * 1971-11-03 1975-10-28 Wisconsin Alumni Res Found NMR method for determination of enantiomeric compositions with chiral shift reagents
WO1991004968A1 (en) * 1989-10-06 1991-04-18 University Of South Carolina Chalcogen-based chiral reagents and method for nuclear magnetic resonance detection of stereochemical assignments and enantiomeric ratios and method for synthesizing said reagents

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MAGNETIC RESONANCE IN MEDICINE., vol.17, no.2, February 1991, DULUTH,MN US pages 516 - 532 S.W.A. BLIGH ET AL. 'USE OF PARAMAGNETIC CHELATED METAL DERIVATIVES OF POLYSACCHARIDES AND SPIN-LABELED POLYSACCHARIDES AS CONTRAST AGENTS IN MAGNETIC RESONANCE IMAGING' *
See also references of WO9514934A1 *
TRAC, TRENDS IN ANALYTICAL CHEMISTRY., vol.12, no.4, April 1993, AMSTERDAM, NL pages 185 - 189 A.F. CASY 'CHIRAL DISCRIMINATION BY NMR SPECTROSCOPY' *

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