EP0641384A1 - VEGETAUX RESISTANT AUX VIRUS ET CONTENANT DES ARNm CYTOTOXIQUES INDUCTIBLES - Google Patents

VEGETAUX RESISTANT AUX VIRUS ET CONTENANT DES ARNm CYTOTOXIQUES INDUCTIBLES

Info

Publication number
EP0641384A1
EP0641384A1 EP93911073A EP93911073A EP0641384A1 EP 0641384 A1 EP0641384 A1 EP 0641384A1 EP 93911073 A EP93911073 A EP 93911073A EP 93911073 A EP93911073 A EP 93911073A EP 0641384 A1 EP0641384 A1 EP 0641384A1
Authority
EP
European Patent Office
Prior art keywords
rna
region
responsive rna
responsive
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP93911073A
Other languages
German (de)
English (en)
Other versions
EP0641384A4 (fr
Inventor
Brent V. Edington
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sirna Therapeutics Inc
Original Assignee
Ribozyme Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ribozyme Pharmaceuticals Inc filed Critical Ribozyme Pharmaceuticals Inc
Publication of EP0641384A1 publication Critical patent/EP0641384A1/fr
Publication of EP0641384A4 publication Critical patent/EP0641384A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/124—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes based on group I or II introns
    • C12N2310/1241—Tetrahymena
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/127—DNAzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification

Definitions

  • the substrate region is part of an exon or a leader region or overlaps the junction between the two (which includes the ribosome recognition sequence, and the initiation codon) , or includes a region necessary for the self-splicing reaction.
  • Fig. 4 is a copy of a photograph of a polyacrylamide gel showing the results of in vitro transcription reactions carried out in the presence of [ ⁇ 32 P]CTP using the parent plasmid (pTETBLU) and the three splicing mutants (pTET14, pTET1419, pTET21-24) as templates.
  • Each set of three lanes represents the transcription products before (0) and after (15 or 60 min) the change to splicing conditions.
  • the template used is given above each set of lanes, and the restriction enzyme used to linearize the template is shown at the top.
  • FL denotes the full-length precursor RNA and LE indicates ligated exons.
  • the regulatory domain may also include a region that will disfavor hybridization of non-specific nucleic acids .i.e.. nucleic acids other than the signal nucleic acid) to the region immediately adjacent to the inhibitor domain.
  • This region is referred to as an inverted repeat and can fold to form a hairpin structure.
  • the detailed nature of the inhibitor region, the substrate region, and the anti-inhibitor region will depend, in part, on the extent that translation is to be regulated. The more stable the intramolecular base-paired domain formed by hybridization of the inhibitor region to the substrate region, the more translation will be inhibited.
  • Intramolecular base pair formation between the inhibitor region and the substrate region prevents hybridization of the 5'-splice junction to the internal guide sequence, and splicing is prevented.
  • This responsive RNA molecule is designed so that the inhibitor region is also complementary to the anti-inhibitor region of the signal nucleic acid.
  • the inhibitor region hybridizes to the anti-inhibitor region freeing the 5'-splice junction for participation in the self-splicing reaction.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

L'invention concerne une molécule d'ARN sensible qui code, dans une ou plusieurs régions de codage de protéines, un polypeptide, et qui comprend un domaine régulateur, une région de substrat et une séquence de reconnaissance ribosomique. Cette molécule d'ARN sensible possède une région inhibitrice dans le domaine régulateur, ce dernier étant complémentaire à la fois d'une région de substrat de la molécule d'ARN sensible et d'une région anti-inhibitrice d'un acide nucléique-signal, de telle manière qu'en l'absence de l'acide nucléique-signal, l'inhibiteur et les régions de substrat forment un domaine apparié par bases, dont la formation réduit le niveau de traduction d'une des régions de codage de protéines dans la molécule d'ARN sensible, par rapport au niveau de traduction de cette région de codage de protéines examinée en présence de l'acide nucléique-signal. La région anti-inhibitrice de l'acide nucléique-signal est complémentaire en séquence de la région inhibitrice de la molécule d'ARN sensible, de telle façon que lorsque la région anti-inhibitrice est appariée par bases à la région inhibitrice, la traduction d'une région de codage de protéines de l'ARN sensible est augmentée en comparaison du niveau de traduction de cette région de codage de protéines examinée en l'absence de l'acide nucléique-signal. L'acide nucléique-signal comprend une partie de l'acide nucléique d'un organisme infectant les cellules végétales.
EP93911073A 1992-05-14 1993-05-06 VEGETAUX RESISTANT AUX VIRUS ET CONTENANT DES ARNm CYTOTOXIQUES INDUCTIBLES. Withdrawn EP0641384A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US88310792A 1992-05-14 1992-05-14
US883107 1992-05-14
PCT/US1993/004240 WO1993023532A1 (fr) 1992-05-14 1993-05-06 VEGETAUX RESISTANT AUX VIRUS ET CONTENANT DES ARNm CYTOTOXIQUES INDUCTIBLES

Publications (2)

Publication Number Publication Date
EP0641384A1 true EP0641384A1 (fr) 1995-03-08
EP0641384A4 EP0641384A4 (fr) 1995-07-12

Family

ID=25382000

Family Applications (1)

Application Number Title Priority Date Filing Date
EP93911073A Withdrawn EP0641384A4 (fr) 1992-05-14 1993-05-06 VEGETAUX RESISTANT AUX VIRUS ET CONTENANT DES ARNm CYTOTOXIQUES INDUCTIBLES.

Country Status (6)

Country Link
EP (1) EP0641384A4 (fr)
JP (1) JPH07507931A (fr)
AU (1) AU4234293A (fr)
CA (1) CA2135643A1 (fr)
MX (1) MX9302804A (fr)
WO (1) WO1993023532A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2188562A1 (fr) * 1994-05-11 1995-11-23 David Allen Jones Procede d'introduction d'une resistance aux agents pathogenes chez les vegetaux
US5712096A (en) * 1994-08-23 1998-01-27 University Of Massachusetts Medical Center Oligoribonucleotide assays for novel antibiotics
US6235974B1 (en) 1996-12-05 2001-05-22 Cornell Research Foundation, Inc. Hypersensitive response induced resistance in plants by seed treatment with a hypersensitive response elicitor
US6998515B1 (en) 1997-01-27 2006-02-14 Cornell Research Foundation, Inc. Use of a nucleic acid encoding a hypersensitive response elicitor polypeptide to enhance growth in plants
US6277814B1 (en) 1997-01-27 2001-08-21 Cornell Research Foundation, Inc. Enhancement of growth in plants
WO1998054214A2 (fr) 1997-05-30 1998-12-03 Cornell Research Foundation, Inc. Fragments eliciteurs de reaction d'hypersensibilite elicitant une reaction d'hypersensibilite, et utilisation de ces fragments
US6228644B1 (en) 1997-08-06 2001-05-08 Cornell Research Foundation, Inc. Hypersensitive response elicitor from Erwinia amylovora, its use, and encoding gene
US6172184B1 (en) 1997-08-06 2001-01-09 Cornell Research Foundation, Inc. Hypersensitive response elicitor from Pseudomonas syringae and its use
US6262018B1 (en) 1997-08-06 2001-07-17 Cornell Research Foundation, Inc. Hypersensitive response elicitor from Erwinia amylovora and its use
US6960705B2 (en) 1998-10-05 2005-11-01 Eden Bioscience Corporation Nucleic acid encoding a hypersensitive response elicitor from Xanthomonas campestris
US6858707B1 (en) 1998-10-05 2005-02-22 Eden Bioscience Corporation Hypersensitive response elicitor fragments which are active but do not elicit a hypersensitive response
US6624139B1 (en) 1998-11-05 2003-09-23 Eden Bioscience Corporation Hypersensitive response elicitor-induced stress resistance

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0298918A2 (fr) * 1987-07-10 1989-01-11 Ciba-Geigy Ag Résistance inductible contre des virus dans des plantes
CN1033645A (zh) * 1988-10-22 1989-07-05 中国科学院上海植物生理研究所 控制植物病毒病的基因工程方法
EP0425004A2 (fr) * 1989-10-03 1991-05-02 Aveve N.V. Manipulations génétiques avec l'ADN recombinant, contenant des séquences dérivées des virus d'ARN
WO1991013994A1 (fr) * 1990-03-13 1991-09-19 Commonwealth Scientific And Industrial Research Organisation Expression de genes
EP0479180A2 (fr) * 1990-10-05 1992-04-08 Hoechst Aktiengesellschaft Plantes résistantes aux virus, procédé pour leur production
WO1992013090A1 (fr) * 1991-01-17 1992-08-06 The General Hospital Corporation Ribozymes de trans-epissage
WO1992013089A1 (fr) * 1991-01-17 1992-08-06 The General Hospital Corporation Ablation de cellules a l'aide de ribozymes de trans-epissage
WO1992013070A1 (fr) * 1991-01-25 1992-08-06 United States Biochemical Corporation Regulation de la traduction de l'acide nucleique

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8601680D0 (en) * 1986-01-23 1986-02-26 Agricultural Genetics Co Modification of plant viruses
ZA885916B (en) * 1987-09-15 1989-04-26 Gen Hospital Corp Pathogenesis-related proteins in plants

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0298918A2 (fr) * 1987-07-10 1989-01-11 Ciba-Geigy Ag Résistance inductible contre des virus dans des plantes
CN1033645A (zh) * 1988-10-22 1989-07-05 中国科学院上海植物生理研究所 控制植物病毒病的基因工程方法
EP0425004A2 (fr) * 1989-10-03 1991-05-02 Aveve N.V. Manipulations génétiques avec l'ADN recombinant, contenant des séquences dérivées des virus d'ARN
WO1991013994A1 (fr) * 1990-03-13 1991-09-19 Commonwealth Scientific And Industrial Research Organisation Expression de genes
EP0479180A2 (fr) * 1990-10-05 1992-04-08 Hoechst Aktiengesellschaft Plantes résistantes aux virus, procédé pour leur production
WO1992013090A1 (fr) * 1991-01-17 1992-08-06 The General Hospital Corporation Ribozymes de trans-epissage
WO1992013089A1 (fr) * 1991-01-17 1992-08-06 The General Hospital Corporation Ablation de cellules a l'aide de ribozymes de trans-epissage
WO1992013070A1 (fr) * 1991-01-25 1992-08-06 United States Biochemical Corporation Regulation de la traduction de l'acide nucleique

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, vol. 113, no. 15, 1990 Columbus, Ohio, US; abstract no. 127723, JUN, W. 'Preparation of transgenic plants for the control of virosis' & CN-A-1 033 645 (FAMING ZHUANLI SHENQUING GONGKAI SHUOMINGSHU) 5 July 1989 *
See also references of WO9323532A1 *

Also Published As

Publication number Publication date
AU4234293A (en) 1993-12-13
WO1993023532A1 (fr) 1993-11-25
CA2135643A1 (fr) 1993-11-25
JPH07507931A (ja) 1995-09-07
MX9302804A (es) 1994-04-29
EP0641384A4 (fr) 1995-07-12

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