EP0638120A1 - Gewebespezifische und entwicklungsregulierte transkriptionssegmenten und ihre verwendungen - Google Patents

Gewebespezifische und entwicklungsregulierte transkriptionssegmenten und ihre verwendungen

Info

Publication number
EP0638120A1
EP0638120A1 EP92921715A EP92921715A EP0638120A1 EP 0638120 A1 EP0638120 A1 EP 0638120A1 EP 92921715 A EP92921715 A EP 92921715A EP 92921715 A EP92921715 A EP 92921715A EP 0638120 A1 EP0638120 A1 EP 0638120A1
Authority
EP
European Patent Office
Prior art keywords
dna
sequence
plant
transcriptional
fruit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP92921715A
Other languages
English (en)
French (fr)
Inventor
Leona Claire Fitzmaurice
T. Erik Mirkov
Kathryn Jane Elliott
Gregory Clyde Holtz
Craig Duane Dickinson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Smart Plants International Inc
Original Assignee
Smart Plants International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Smart Plants International Inc filed Critical Smart Plants International Inc
Publication of EP0638120A1 publication Critical patent/EP0638120A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8235—Fruit-specific
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Definitions

  • the transcriptional regions may be native or homologous to the host or foreign or heterologous to the host. By foreign it is intended that the transcriptional sequence is not found in the wild-type host into which the transcriptional sequence is introduced.
  • tissue-specific ⁇ e.g, fruit transcriptional initiation regions of clone ⁇ UC82-3.3 SEQ ID NO. 2.
  • this transcriptional region is activated upon or shortly after the onset of fruit ripening and remains active during the red fruit stage, peaking approximately midway during the ripening process.
  • the termination region which is employed may be native with respect to the transcriptional. sequence, may be native with respect to the coding DNA sequence, or may be derived from another source. Examples of termination regions from other sources include the octopine synthase and nopaline synthase termination regions derived from the Ti-plasmid of A. tumefaciens.
  • RNA was prepared from 1-inch green and 3-inch intermediate developmental stages of L. esculentum cv. UC82 fruit as described above.
  • the RNAs were subjected to electrophoresis on a 1.5% agarose gel prepared in 1.1 M formaldehyde, 10 mM NaP0 4 , pH 7.4, and electrophoresed in the same buffer.
  • the RNA was transferred to a nitrocellulose filter essentially as described by Thomas (Proc. Natl. Acad. Sci. USA 77:5201-5205, 1980).
  • Lycopersicon esculentum SEQUENCE ID NO. 2 a genomic clone of ⁇ UC82-3.3 from Lycopersicon esculentum

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
EP92921715A 1991-10-04 1992-10-02 Gewebespezifische und entwicklungsregulierte transkriptionssegmenten und ihre verwendungen Withdrawn EP0638120A1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US77008091A 1991-10-04 1991-10-04
US770080 1991-10-04
PCT/US1992/008425 WO1993007257A2 (en) 1991-10-04 1992-10-02 Tissue-specific and developmentally regulated transcriptional sequences and uses thereof

Publications (1)

Publication Number Publication Date
EP0638120A1 true EP0638120A1 (de) 1995-02-15

Family

ID=25087409

Family Applications (1)

Application Number Title Priority Date Filing Date
EP92921715A Withdrawn EP0638120A1 (de) 1991-10-04 1992-10-02 Gewebespezifische und entwicklungsregulierte transkriptionssegmenten und ihre verwendungen

Country Status (3)

Country Link
EP (1) EP0638120A1 (de)
AU (2) AU2807592A (de)
WO (1) WO1993007257A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL100908A0 (en) * 1991-02-22 1992-11-15 Salk Inst Biotech Ind Invertase genes and their use
GB9305861D0 (en) * 1993-03-22 1993-05-12 Zeneca Ltd Dna,dna constructs,cells and plants derived therefrom
US6291744B1 (en) 1994-08-12 2001-09-18 Dna Plant Technology Corporation Nucleic acids encoding plant group 2 proteins and uses thereof
US5608144A (en) * 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5536653A (en) * 1994-11-04 1996-07-16 Monsanto Company Tomato fruit promoters
EP0858507A2 (de) * 1995-10-12 1998-08-19 The Regents of The University of Michigan Verwendung der linalool-synthase zur genetischen manipulation der duftproduktion
NZ500741A (en) * 1997-04-09 2001-06-29 Ministry Of Agriculture And Fo Inducible plant promoters selected from apple beta-galactosidase (ABG1) or 1-aminocyclopropane-1-carboxylate synthase (ACC synthase)
KR101101774B1 (ko) * 2009-02-12 2012-01-05 전남대학교산학협력단 토마토 히스티딘 디카르복실라제 유전자 유래 열매 특이적 발현 프로모터 및 이의 용도

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0416032A4 (en) * 1988-05-20 1992-11-25 The Salk Institute Biotechnology Industrial Associates, Inc. Agrichemical screens
WO1991001373A1 (en) * 1989-07-19 1991-02-07 Calgene, Inc. Fruit-specific transcriptional factors
ES2166750T3 (es) * 1989-07-19 2002-05-01 Calgene Llc Composiciones y procedimientos para la modulacion de niveles de citoquinina endogenas.
IL100908A0 (en) * 1991-02-22 1992-11-15 Salk Inst Biotech Ind Invertase genes and their use

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9307257A3 *

Also Published As

Publication number Publication date
WO1993007257A2 (en) 1993-04-15
AU2371897A (en) 1997-08-28
AU2807592A (en) 1993-05-03
WO1993007257A3 (en) 1993-08-05

Similar Documents

Publication Publication Date Title
US6610840B2 (en) Method of isolating a mesophyll-specific promoter
US4943674A (en) Fruit specific transcriptional factors
CA2006454C (en) Potatoe tuber specific transcriptional regulation
KR100228918B1 (ko) 헬리안티닌의 상부 조절요소를 기초로 한 키메릭 식물 유전자
US6329570B1 (en) Cotton modification using ovary-tissue transcriptional factors
CA2285687C (en) An oleosin 5' regulatory region for the modification of plant seed lipid composition
US6080914A (en) Strawberry promoters and genes
AU636563B2 (en) Fruit-specific transcriptional factors
US5436394A (en) Plasmid for the preparation of transgenic plants with a change in habit and yield
US5576428A (en) Invertase gene(s) and uses thereof
US5175095A (en) Ovary-tissue transcriptional factors
KR19990082127A (ko) 식물 내의 트랜스유전자의 조절 발현을 위한 식물 조직/단계 특이적 프로모터
CA2078229A1 (en) Plant promoter
NZ517467A (en) Flax seed specific promoters and methods for specifically expressing genes of interest in flax seeds
WO1993006711A1 (en) Tomato acid invertase gene
US5847102A (en) Cold induced promoter from winter Brassica napus
EP0876481A1 (de) Gene für früchtereifung
WO1993007257A2 (en) Tissue-specific and developmentally regulated transcriptional sequences and uses thereof
US5917127A (en) Plasmids useful for and methods of preparing transgenic plants with modifications of habit and yield
US5723757A (en) Plant promoters specific for sink organ expression of genes
US6025544A (en) Processes for modifying plant flowering behavior
US7557264B2 (en) Gossypium hirsutum tissue-specific promoters and their use
AU6552599A (en) Tissue-specific and developmentally regulated transcriptional sequences and uses thereof
AU754135B2 (en) Gene promoter sequences and uses thereof
US5824865A (en) Chimeric plant genes based on upstream regulatory elements of helianthinin

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 19940428

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL SE

GRAG Despatch of communication of intention to grant

Free format text: ORIGINAL CODE: EPIDOS AGRA

RIC1 Information provided on ipc code assigned before grant

Free format text: 7C 12N 15/82 A, 7C 12N 9/14 B, 7C 12N 15/55 B, 7A 01H 3/00 B, 7A 01H 5/00 B

17Q First examination report despatched

Effective date: 20001127

RIC1 Information provided on ipc code assigned before grant

Free format text: 7C 12N 15/82 A, 7C 12N 9/14 B, 7C 12N 15/55 B, 7A 01H 3/00 B, 7A 01H 5/00 B

GRAG Despatch of communication of intention to grant

Free format text: ORIGINAL CODE: EPIDOS AGRA

GRAH Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOS IGRA

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20010503