EP0638093A1 - Novel plant virus sequences - Google Patents
Novel plant virus sequencesInfo
- Publication number
- EP0638093A1 EP0638093A1 EP93910160A EP93910160A EP0638093A1 EP 0638093 A1 EP0638093 A1 EP 0638093A1 EP 93910160 A EP93910160 A EP 93910160A EP 93910160 A EP93910160 A EP 93910160A EP 0638093 A1 EP0638093 A1 EP 0638093A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- sequence
- amino acid
- acid sequence
- nucleotide sequence
- bammv
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/34011—Potyviridae
- C12N2770/34022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Definitions
- This invention relates to novel plant virus sequences and concerns novel amino acid sequences, novel nucleotide sequences, vectors comprising the novel nucleotide sequences, plants into which the novel sequences have been introduced, a method of increasing the resistance of plants to disease and transgenic plants having increased resistance to disease.
- Barley yellow mosaic disease is one of the most important diseases of winter barley in central European and Asiatic countries (Inouye & saito, 1975 Descriptions of Plant Viruses, No. 143). It has recently been recognised that the disease is caused by two viruses: barley yellow mosaic virus (BaYMV) and barley mild mosaic virus (BaMMV; Kashiwazaki et al . , 1989 Annals Phytopathology Society Japan 5_5_, 16-25; Huth and Adams, 1990 Intervirology 3_1, 38-42; Prols et al. , 1990 Journal of Phytopathology 130, 249-259), although it was previously thought that BaMMV was a mechanically transmissible isolate of BaYMV.
- BaYMV barley yellow mosaic virus
- BaMMV barley mild mosaic virus
- the two viruses have identical particle morphology being composed of flexuous rods with two modal lengths of approximately 275nm and 550n ⁇ n and produce similar symptoms (Huth et al., 1984 Phytopathology Z 1H, 37-54).
- the particles encapsidate bipartite, polyadenylated single
- a complementary approach is to create resistance via a genetic engineering route once gene transfer methods have been developed for cereal crops.
- a variety of molecular techniques have been used to generate plants resistant to a particular virus, including coat-protein mediated cross- protection.
- the invention provides an amino acid sequence comprising the amino acid sequence of residues 1- 123 (Seq-. ID No. 1) of Figure 4 or functional equivalents thereof.
- the invention provides an amino acid sequence comprising the amino acid sequence of residues 124-374 (Seq. ID No. 2) of Figure 4 or functional equivalents thereof.
- This sequence encompasses the coat polypeptide of BaMMV.
- expression of viral coat proteins by plants can increase the resistance of those plants to disease caused by that virus.
- the invention provides an amino acid sequence comprising the amino acid sequence of residues 1- 374 of Figure 4 (Seq. ID No. 3) or functional equivalents thereof.
- the invention provides a nucleotide sequence which comprises a sequence encoding the amino acid sequence of amino acids 124-374 of Figure 4 or functional equivalents thereof.
- nucleotide sequences encoding the amino acid sequence of amino acids 124-374 of Figure 4 comprise an in-frame ATG translation start codon and an appropriate initiation consensus sequence at the 5' end of the sequence.
- the nucleotide sequence of the invention comprises the nucleotide sequence of nucleotides 370-1122 of the sequence of Figure 4 (Seq. ID No. 4).
- the invention provides the sequence of nucleotides 370-1462 (Seq. ID No. 6) of the nucleotide sequence of Figure 4 or functional equivalents thereof.
- the invention provides a nucleotide sequence comprising a sequence encoding the amino acid sequence of amino acids 1-123 of Figure 4, or functional equivalents thereof.
- nucleotide sequence in accordance with this aspect of the invention comprises the sequence of nucleotides 1-369 (Seq. ID No. 7) of Figure 4.
- nucleotide sequences of the invention comprise a 3 1 poly A tail.
- the vector defined above includes a promoter suitable for the intended host and therefore should be capable of expressing a polypeptide encoded by the nucleotide sequence(s) of the invention.
- the invention provides a plant or part thereof into which a nucleotide sequence of the invention has been artificially introduced.
- transgenic plants containing viral sequences from a particular virus which are transcribed only, or transcribed and then translated, are rendered more resistant to disease caused by that particular virus.
- viral sequences from a particular virus
- transcribing or translating the sequence of the invention would be more resistant to disease caused by barley mild mosaic virus.
- the increase in resistance may be exhibited as total immunity to the virus or as the delayed onset of symptoms, or any stage between those two extremes (e.g. reduced severity of symptoms).
- the invention provides a method of rendering plants more resistant to disease caused by barley mild mosaic virus comprising transforming a host plant or part thereof with a vector comprising a nucleotide sequence of the invention, such that the sequence of the invention is transcribed within the host plant.
- the invention provides a method of rendering plants more resistant to disease caused by barley mild mosaic virus comprising transforming a host plant or part thereof with a vector comprising a nucleotide sequence encoding the amino acid sequence of amino acids 124-374 of Figure 4.
- the invention provides a method of rendering plants more resistant to disease caused by barley mild mosaic virus comprising transforming a host plant or part thereof with a vector comprising a nucleotide sequence encoding the amino acid sequence of amino acids 1-123 of Figure 4.
- the vector comprises the sequence of nucleotides 1-369 of Figure 4.
- the invention provides a transgenic plant, into which has been artificially introduced a nucleotide sequence encoding the amino acid sequence of Figure 4 or functional equivalents thereof, with increased resistance to disease caused by barley mild mosaic virus.
- Figure 1 shows a photograph of SDS-PAGE analysis of BaMMV coat protein polypeptides from purified virus particles
- Figure 3 is a schematic representation of the sequencing strategy used to determine the DNA sequence of the BaMMV coat protein
- the Streatley strain of BaMMV was originally obtained from Dr M J Adams (Rotha sted, UK) and was propagated in Hordeum vulgare cultivar Maris Otter by mechanical inoculation using an artist's airbrush (Adams et al,, 1986 Annals of Applied Biology 109, 561-572).
- Virus was purified as described by Huth et al., (1984) but with the addition of ImM phenyl methyl sulphonyl fluoride to prevent degradation.
- BaMMV RNA was isolated from the purified virus by incubation in O.lmg/ml proteinase K, 0.1% SDS for 20 minutes at room temperature followed by phenol-chloroform extraction and ethanol precipitation.
- Plasmid DNA from ampicillin-resistant colonies was prepared by the method of Holmes and Quigley (1981 Anal. Biochem. 114, 193-197) and screened by restriction mapping. Gels were also Southern blotted and probed with CP-1 and CP-2.
- the 3' untranslated region of BaMMV (340nts) is longer than that of BaYMV (231nts) and contains a direct repeat of approximately 120nts with short palindromic sequences. Repeats within the 3' untranslated region have been observed in other potyviruses (Dougherty et al., 1985, Virology 146, 282-291; Hay et al, 1989 Archives of Virology 107, 11-122). In addition, there is a potential polyadenylation motif (UAUGU) 85nts upstream from the poly (A) tail ( Figure 4) which has also been noted in the sequence of potyviruses (Maiss et al, 1989 Journal of General Virology 7_0, 513-524).
- UAUGU polyadenylation motif
- PCR was performed using Bam 3 and BMCP-1 on cDNA and the products cloned as a BamHI-Bqlll (using the Bglll site in coat protein at nt 821) fragment into the BamHI site of Bluescript (Stratagene).
- Clones were then sequenced in full to verify addition of the extra sequences and to confirm that PCR had not introduced any base changes.
- the coat protein region of the resulting clone (pMP-5) was found to be identical in sequence to the same region of pBM-217 and the extra cases at the 5' end had been correctly added.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9209669 | 1992-05-02 | ||
GB929209669A GB9209669D0 (en) | 1992-05-02 | 1992-05-02 | Novel plant virus sequences |
PCT/GB1993/000910 WO1993022345A1 (en) | 1992-05-02 | 1993-04-30 | Novel plant virus sequences |
Publications (1)
Publication Number | Publication Date |
---|---|
EP0638093A1 true EP0638093A1 (en) | 1995-02-15 |
Family
ID=10715013
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP93910160A Withdrawn EP0638093A1 (en) | 1992-05-02 | 1993-04-30 | Novel plant virus sequences |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0638093A1 (en) |
JP (1) | JPH07506251A (en) |
AU (1) | AU4077293A (en) |
CA (1) | CA2134784A1 (en) |
GB (1) | GB9209669D0 (en) |
WO (1) | WO1993022345A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05344888A (en) * | 1992-06-16 | 1993-12-27 | Norin Suisansyo Nogyo Kenkyu Center Shocho | Barley mild mosaic virus gene |
JPH06276871A (en) * | 1993-03-29 | 1994-10-04 | Norin Suisansyo Nogyo Kenkyu Center Shocho | Production of barley resistant to yellow mosaic disease |
-
1992
- 1992-05-02 GB GB929209669A patent/GB9209669D0/en active Pending
-
1993
- 1993-04-30 CA CA 2134784 patent/CA2134784A1/en not_active Abandoned
- 1993-04-30 WO PCT/GB1993/000910 patent/WO1993022345A1/en not_active Application Discontinuation
- 1993-04-30 AU AU40772/93A patent/AU4077293A/en not_active Abandoned
- 1993-04-30 JP JP5519089A patent/JPH07506251A/en active Pending
- 1993-04-30 EP EP93910160A patent/EP0638093A1/en not_active Withdrawn
Non-Patent Citations (1)
Title |
---|
See references of WO9322345A1 * |
Also Published As
Publication number | Publication date |
---|---|
WO1993022345A1 (en) | 1993-11-11 |
JPH07506251A (en) | 1995-07-13 |
CA2134784A1 (en) | 1993-11-11 |
GB9209669D0 (en) | 1992-06-17 |
AU4077293A (en) | 1993-11-29 |
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Legal Events
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Inventor name: SLABAS, ANTONI RYSZARD Inventor name: SIDEBOTTOM, CHRISTOPHER, MICHAEL Inventor name: LEA, VINCENT JOHN Inventor name: JAMES, CHRISTOPHER, MICHAEL Inventor name: JACK, PETER, LIAM Inventor name: FOULDS, IAN, JEFFREY Inventor name: BREARS, TIMOTHY Inventor name: BOULTON, ROBERT EDWIN |
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RAP1 | Party data changed (applicant data changed or rights of an application transferred) |
Owner name: THE MONSANTO COMPANY |
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Effective date: 19991129 |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
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18D | Application deemed to be withdrawn |
Effective date: 20000411 |