EP0632749B1 - Liquid-dosing tube - Google Patents
Liquid-dosing tube Download PDFInfo
- Publication number
- EP0632749B1 EP0632749B1 EP93920526A EP93920526A EP0632749B1 EP 0632749 B1 EP0632749 B1 EP 0632749B1 EP 93920526 A EP93920526 A EP 93920526A EP 93920526 A EP93920526 A EP 93920526A EP 0632749 B1 EP0632749 B1 EP 0632749B1
- Authority
- EP
- European Patent Office
- Prior art keywords
- tube
- laboratory test
- test tube
- liquid
- laboratory
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 239000013049 sediment Substances 0.000 claims description 13
- 230000014759 maintenance of location Effects 0.000 claims description 12
- 230000008719 thickening Effects 0.000 claims description 9
- 238000009533 lab test Methods 0.000 claims 11
- 229920002994 synthetic fiber Polymers 0.000 claims 1
- 239000012895 dilution Substances 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000011521 glass Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000003708 ampul Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 238000007792 addition Methods 0.000 description 1
- 210000003323 beak Anatomy 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
Definitions
- the present invention relates to a laboratory tube for dosing liquids with which it is possible, in a simple, user-friendly and contamination-safe manner, to retain a portion of a liquid in a predetermined amount in the laboratory tube and to remove the rest. With this device it is possible on the one hand to concentrate sedimentable solids and on the other hand to dilute dissolved substances.
- US-A 3,748,099 describes a laboratory tube with fill level markings and sealing plugs (14), which adjoins the inside or outside of the tube.
- a cannula 23 is slidably inserted, through which defined amounts of liquid can be squeezed out.
- the device shown also serves more as a pipette with which defined amounts of solutions can be dispensed and not as a laboratory tube for metering liquids which are retained in the tube while an excess is being removed.
- GB-A 20 83 091 describes a tube which is open on both sides, a narrow inner channel (40) being formed either by an inserted stopper or by a corresponding shaping of the tube, through which liquids are filled or drawn off can and in the outer area of the solid accumulates during centrifugation.
- the channel must be very narrow in accordance with the procedure, since otherwise a noticeable amount of the solid matter is inside the channel during centrifugation and would be lost accordingly.
- US-A 3,481,712 describes a centrifuge tube with a tapered lower end (25) in which the sediment is held while the supernatant liquid can be removed.
- the closure flap (21) is designed as a microscope glass, so that the sediment can then be poured directly onto the plate, as shown in FIG. 8, an excess running into the outer cavity (35). This does not involve a measurement.
- FIGS. 9 and 10 An alternative embodiment is described in FIGS. 9 and 10, the sediments settling in the intermediate space of the cap, from which, after the liquid has been removed, they can be transferred again to a microscope plate by gentle shaking, as shown in FIG. Even with this procedure, there is no constant retention volume for liquid which is to be returned to the laboratory tube and mixed there.
- FR-A 2.122.187 describes an ampoule with parts of different widths so that an easy measurement of the liquids in these parts is possible.
- a cannula is also inserted into the upper opening, through which it is possible to draw a certain volume of liquid into the ampoule.
- the inwardly protruding tube (56) allows air to be expelled from the ampoule in the event of multiple suction processes, or an excess of liquid to be pressed out again.
- an inner tube is inserted into the laboratory tube, which has a cylindrical opening, the outer diameter of which is 0.5-2 mm smaller than the inner diameter of the cylindrical opening, so that a defined space is formed which, depending on the length of the inserted inner tube, defines a retention space .
- This retention space fills with the slowly pouring out the contents of the laboratory tube with the outflowing liquid, so that only the excess runs off and at Returning the tube to the vertical completely runs out of this space.
- the space In order to allow a complete reflux, the space must not be so narrow that capillary forces hold part of the liquid when it is reset, which results in the lower limit of about 0.5-1 mm.
- the gap should not become too wide either, since otherwise the length of the inner tube becomes correspondingly smaller for a certain retention volume and additional errors can occur when emptying.
- the inner opening of the inserted inner tube used for filling and emptying becomes correspondingly small, which in turn can lead to handling disadvantages. It is therefore advantageous to expand this opening in a funnel shape or, if desired, to provide it with a pouring spout.
- the firm connection between the laboratory tube and the inner tube is preferably brought about in that the inner tube has a corresponding thickening in its upper part, which can be inserted into the opening of the laboratory tube with a certain pressure.
- a molded collar can be provided to prevent the tube from being pressed too deeply.
- the thickening inside can then be dispensed with.
- laboratory tubes are made almost entirely of glass or plastic and can be manufactured with very small tolerances.
- the inner tubes used according to the invention are preferably also made of plastic, which on the one hand has sufficient elasticity to form a tight connection with the laboratory tube and on the other hand is so inexpensive that the entire device is manufactured as a disposable article and can therefore be thrown away after use. If the laboratory tube and inner tube are made of the same plastic, there is also the advantageous possibility of recycling the plastic without the two parts must be separated again.
- the present invention is particularly intended for the commercially available centrifuge tubes, which have a content of about 15 ml and e.g. with markings for 3, 5 and 10 ml. With a length of about 10 cm, such tubes have an inner diameter of 14 mm. An inserted inner tube with an outer diameter of 1.2 mm and a length of 15 mm has a retention volume of 1 cm3. Using the specified markings, dilution or concentration ratios of 1: 3, 1: 5 or 1:10 can therefore be set very easily without additional measuring aids.
- the same device can of course also be used for differently shaped vessels as long as they have an opening which fits exactly with the device.
- the usual 10 ml syringe made of plastic which has a shortenable piston shaft for centrifuging, can also be provided with such an inner tube after centrifuging and in this way not only a defined amount of serum but also a defined amount of the sediment resuspended in the serum be obtained, which is available for further investigation.
- Other laboratory vessels such as Erlenmeyer flasks and round-bottom flasks etc., which have a cylindrical opening with a suitable inner diameter, can also be converted into a measuring vessel by attaching a corresponding retention device.
- Other forms of application are conceivable without being listed in detail here.
- FIG. 1 shows a centrifuge glass with a retention system inserted, FIG. 1a being a section through the system, FIG. 1b showing the filled tube, FIG. 1c showing the inclined tube with the escaping liquid, FIG. 1d shows the tube in the emptied state with the remaining volume in the retaining device and FIG. 1e shows the turned-back tube with the remaining volume on the sediment.
- Figure 2 is an enlarged representation of Figure 1e with the laboratory tube 1, the inserted inner tube 2 which has a thickening 3 which fits into the inner diameter of the laboratory tube 1 and a collar 4 which surrounds the laboratory tube 1 on the outside.
- a funnel-shaped extension 5 is incorporated in the thickening 3 and the collar 4, a funnel-shaped extension 5 is incorporated.
- the space between the inner tube 2 and the laboratory tube 1 defines the retention volume 7, which corresponds to the volume 7a in the reset state.
- a sediment 8 is indicated at the bottom of the tube 2.
- 2 fill level markings 6 are indicated, which correspond, for example, to a fill quantity of 3 cm 3.
- Figure 3 shows a simplified embodiment of the inner tube, which consists only of the inner tube 2 and the molded thickening 3.
- FIG. 4 shows a device which, in addition to the inner tube 2, the thickening 3 and the collar 4, also has a molded spout 9.
- FIG. 5 shows a commercially available syringe with the outer tube 10, the piston 11, the handle 13 provided with several notches 12 and a screw-on closure cap 14 to which the cannula attachment 15 is molded.
- FIG. 5a shows the same syringe with the attachment according to the invention, which is screwed onto the syringe via a screw connection 16.
- the handle 13 has broken off at the first notch 12.
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Devices For Use In Laboratory Experiments (AREA)
- Sampling And Sample Adjustment (AREA)
- Infusion, Injection, And Reservoir Apparatuses (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Die vorliegende Erfindung betrifft ein Laborröhrchen für die Dosierung von Flüssigkeiten mit dem es möglich ist, einfach, anwendungsfreundlich und kontaminationssicher, einen Teil einer Flüssigkeit, in einer vorbestimmten Menge, im Laborröhrchen zurückzuhalten und den Rest zu entfernen. Mit dieser Vorrichtung ist es einerseits möglich sedimentierbare Feststoffe zu konzentrieren und andererseits gelöste Stoffe zu verdünnen.The present invention relates to a laboratory tube for dosing liquids with which it is possible, in a simple, user-friendly and contamination-safe manner, to retain a portion of a liquid in a predetermined amount in the laboratory tube and to remove the rest. With this device it is possible on the one hand to concentrate sedimentable solids and on the other hand to dilute dissolved substances.
Bei der Untersuchung von sedimentierbaren Inhaltsstoffen einer Flüssigkeit, beispielsweise Urin oder Blut, wird eine bestimmte Menge der Suspension in ein Zentrifugenglas gefüllt, der Feststoff durch Zentrifugieren am Boden des Glases gesammelt, die überstehende Lösung abgegossen und das Sediment zur weiteren Untersuchung in einer bestimmten, normalerweise wesentlich kleineren Menge Flüssigkeit resuspendiert, um ein konzentrierteres Sediment zu erhalten. Zum Wiederaufnehmen wird dabei normalerweise die gleiche Flüssigkeit benutzt die vorher abgegossen wurde. Dazu muß eine definierte Menge abpipettiert und wieder in das Laborröhrchen zurückgeführt werden. Außer der Arbeit die dieses verursacht, besteht durch das Handhaben von Flüssigkeiten, welche ggf. Krankheitserreger enthalten, auch die Gefahr einer Kontamination.When examining sedimentable constituents of a liquid, such as urine or blood, a certain amount of the suspension is filled into a centrifuge glass, the solid is collected by centrifuging at the bottom of the glass, the supernatant solution is poured off and the sediment is analyzed in a certain, usually resuspended much smaller amount of liquid to obtain a more concentrated sediment. The same liquid that was previously poured off is normally used to resume. To do this, a defined amount must be pipetted off and returned to the laboratory tube. In addition to the work this causes, there is also a risk of contamination from handling liquids that may contain pathogens.
Umgekehrt ist es häufig nicht möglich Lösungen direkt zu untersuchen, weil die Konzentration der Inhaltsstoffe für die Untersuchungsmethoden zu hoch liegt, um differenzierbare Ergebnisse zu erhalten. Für diesen Fall ist es notwendig die Lösungen zu verdünnen, was wiederum über eine Reihe von Pipettierschritten durchgeführt wird. Zur Vereinfachung dieser Verdünnung wurde daher in der DE-G 19 72 298 vorgeschlagen, am Boden eines Laborröhrchens ein enges Sackloch vor-zusehen, in dem sich durch Kapillarkräfte beim Ausleeren einer größeren Menge eine geringe definierte Flüssigkeitsmenge fängt, die sich beim Wiederauffüllen mit Verdünnungsmittel, durch die beim Einfüllen bewirkten Turbulenzen und Konzentrationsgradienten in dem Verdünnungsmittel, gleichmäßig verteilt. Durch entsprechende Markierungen des Laborröhrchens lassen sich definierte Verdünnungsmittelzugaben und damit Verdünnungsreihen leicht und ohne zusätzliche Hilfsmittel herstellen. Dies Verfahren hat jedoch die Nachteile, daß einerseits durch die Kapillarkräfte nur sehr geringe Flüssigkeitsmengen zurückgehalten werden können, so daß entweder der Verdünnungsfaktor sehr groß oder die nach dem Verdünnen erhaltene Lösungsmenge relativ klein ist und andererseits das Herstellen reproduzierbarer Kapillarräume einen erheblichen technischen Fertigungsaufwand erfordert, so daß es nicht möglich ist, solche Röhrchen als Wegwerf-Artikel zu fertigen und beim Reinigen wieder die Gefahr einer Kontamination auftritt. Darüberhinaus ist ein solches System nicht geeignet zur Konzentration von Sedimenten, da ein Feststoff aus dem Kapillarsystem nicht reproduzierbar wieder herausgelöst werden kann.Conversely, it is often not possible to investigate solutions directly because the concentration of the ingredients is too high for the investigation methods to obtain differentiable results. In this case it is necessary to dilute the solutions, which in turn is carried out via a series of pipetting steps. To simplify this dilution, it was therefore proposed in DE-G 19 72 298 to provide a narrow blind hole at the bottom of a laboratory tube, in which a small defined amount of liquid is caught by capillary forces when emptying a larger amount, which is found when refilling with thinner, due to the turbulence and concentration gradients in the diluent during filling, equally distributed. Appropriate markings on the laboratory tube allow defined additions of diluents and thus dilution series to be prepared easily and without additional aids. However, this method has the disadvantages that on the one hand only very small amounts of liquid can be retained by the capillary forces, so that either the dilution factor is very large or the amount of solution obtained after dilution is relatively small and on the other hand the production of reproducible capillary spaces requires a considerable technical outlay that it is not possible to manufacture such tubes as disposable items and that the risk of contamination occurs again when cleaning. In addition, such a system is not suitable for the concentration of sediments, since a solid cannot be released from the capillary system in a reproducible manner.
Es stellte sich daher die Aufgabe eine einfache Vorrichtung zu schaffen, welche einerseits eine definierte Menge einer Flüssigkeit in einem Laborröhrchen zurückhält und andererseits ein vollständiges Durchmischen mit zurückgebliebenem Sediment oder zugesetzter Verdünnungslösung erlaubt.It was therefore the task of creating a simple device which, on the one hand, holds back a defined amount of a liquid in a laboratory tube and, on the other hand, allows complete mixing with remaining sediment or added dilution solution.
Diese Aufgabe wird durch die Merkmale des Hauptanspruchs gelöst und durch die Merkmale der Unteransprüche gefördert.This object is achieved by the features of the main claim and promoted by the features of the subclaims.
US-A 3.748.099 beschreibt ein Laborröhrchen mit Füllstands-Markierungen und Verschlußstopfen (14), welcher innen oder außen um das Röhrchen dicht anschließt. In diesem ist verschiebbar eine Kanüle 23 eingesetzt, durch die definierte Flüssigkeitsmengen ausgepreßt werden können. Die dargestellte Vorrichtung dient insofern auch mehr als Pipette, mit der definierte Mengen von Lösungen abgegeben werden können und nicht als Laborröhrchen zur Dosierung von Flüssigkeiten die im Röhrchen zurückgehalten werden, während ein Überschuß entfernt wird.US-A 3,748,099 describes a laboratory tube with fill level markings and sealing plugs (14), which adjoins the inside or outside of the tube. In this a cannula 23 is slidably inserted, through which defined amounts of liquid can be squeezed out. In this respect, the device shown also serves more as a pipette with which defined amounts of solutions can be dispensed and not as a laboratory tube for metering liquids which are retained in the tube while an excess is being removed.
GB-A 20 83 091 beschreibt ein Röhrchen, welches an beiden Seiten offen ist, wobei entweder durch einen eingesetzten Stopfen oder durch eine entsprechende Ausformung des Röhrchens selbst ein schmaler innerer Kanal (40) gebildet wird, durch den Flüssigkeiten eingefüllt oder abgezogen werden können und in dessen Außenbereich sich beim Zentrifugieren der Feststoff ansammelt. Der Kanal muß der Vorgehensweise entsprechend sehr eng sein, da sonst beim Zentrifugieren eine merkliche Menge des festen Stoffes sich innerhalb des Kanals befinden und entsprechend verloren gehen würde.GB-A 20 83 091 describes a tube which is open on both sides, a narrow inner channel (40) being formed either by an inserted stopper or by a corresponding shaping of the tube, through which liquids are filled or drawn off can and in the outer area of the solid accumulates during centrifugation. The channel must be very narrow in accordance with the procedure, since otherwise a noticeable amount of the solid matter is inside the channel during centrifugation and would be lost accordingly.
US-A 3,481,712 beschreibt ein Zentrifugenglas mit einem verjüngten unteren Ende (25) in dem das Sediment festgehalten wird, während die überstehende Flüssigkeit entfernt werden kann. Die Verschlußklappe (21) ist in ihrem planen oberen Teil als Mikroskopierglas ausgebildet, so daß anschließend, wie in Figur 8 dargestellt, das Sediment direkt auf die Platte aufgegossen werden kann, wobei ein Überschuß in den äußeren Hohlraum (35) läuft. Eine Messung ist damit nicht verbunden. Eine alternative Ausführungsweise wird in Figuren 9 und 10 beschrieben, wobei die Sedimente sich in dem Zwischenraum der Kappe absetzen, aus dem sie nach Entfernen der Flüssigkeit durch leichtes Schütteln, wie in Figur 10 dargestellt, wieder auf eine Mikroskopierplatte überführt werden können. Auch bei dieser Vorgehensweise ist ein konstantes Rückhaltevolumen für Flüssigkeit, die wieder in das Laborröhrchen zurückgeleitet und dort vermischt werden soll, nicht gegeben.US-A 3,481,712 describes a centrifuge tube with a tapered lower end (25) in which the sediment is held while the supernatant liquid can be removed. In its flat upper part, the closure flap (21) is designed as a microscope glass, so that the sediment can then be poured directly onto the plate, as shown in FIG. 8, an excess running into the outer cavity (35). This does not involve a measurement. An alternative embodiment is described in FIGS. 9 and 10, the sediments settling in the intermediate space of the cap, from which, after the liquid has been removed, they can be transferred again to a microscope plate by gentle shaking, as shown in FIG. Even with this procedure, there is no constant retention volume for liquid which is to be returned to the laboratory tube and mixed there.
FR-A 2.122.187 beschreibt eine Ampulle mit verschieden weiten Teilen, so daß eine leichte Messung der in diesen Teilen stehenden Flüssigkeiten möglich ist. In der Figur 10 ist darüber hinaus noch eine Kanüle in die obere Öffnung eingesetzt, durch welche es möglich ist, ein gewisses Flüssigkeitsvolumen in die Ampulle einzusaugen. Die nach innen überstehende Röhre (56) erlaubt es dabei, bei mehrfachen Ansaugvorgängen zwischenzeitlich Luft aus der Ampulle wieder auszustoßen, bzw. ein Überschuß Flüssigkeit wieder herauszudrücken.FR-A 2.122.187 describes an ampoule with parts of different widths so that an easy measurement of the liquids in these parts is possible. In FIG. 10, a cannula is also inserted into the upper opening, through which it is possible to draw a certain volume of liquid into the ampoule. The inwardly protruding tube (56) allows air to be expelled from the ampoule in the event of multiple suction processes, or an excess of liquid to be pressed out again.
Erfindungsgemäß wird in das Laborröhrchen, welches eine zylinderförmige Öffnung besitzt ein Innenröhrchen eingefügt, dessen Außendurchmesser 0,5-2 mm kleiner ist als der Innendurchmesser der zylindrischen Öffnung, so daß sich ein definierter Zwischenraum bildet, welcher je nach Länge des eingeschobenen Innenröhrchen einen Rückhalteraum definiert. Dieser Rückhalteraum füllt sich beim langsamen Ausgießen des Inhalts des Laborröhrchens mit der ausfließenden Flüssigkeit, so daß nur der Überschuß abläuft und beim Wiederzurückführen des Röhrchens in die Senkrechte vollständig aus diesem Zwischenraum ausläuft. Um einen vollständigen Rücklauf zu ermöglichen darf der Zwischenraum daher nicht so eng sein, daß Kapillarkräfte einen Teil der Flüssigkeit beim Zurückstellen festhalten, wodurch sich die untere Grenze von etwa 0,5-1 mm ergibt. Andererseits sollte der Spalt jedoch auch nicht zu breit werden, da sonst für ein bestimmtes Rückhaltevolumen die Länge des Innenrohres entsprechend kleiner wird und damit zusätzliche Fehler beim Entleeren auftreten können. Darüberhinaus wird bei von Haus aus engen Laborröhrchen die zum Befüllen und Entleeren dienende Innenöffnung des eingeschobenen Innenrohrs entsprechend klein, was wiederum Handhabungsnachteile mit sich bringen kann. Es ist daher vorteilhaft diese Öffnung trichterförmig zu erweitern oder falls gewünscht auch mit einem Ausgießschmabel zu versehen.According to the invention, an inner tube is inserted into the laboratory tube, which has a cylindrical opening, the outer diameter of which is 0.5-2 mm smaller than the inner diameter of the cylindrical opening, so that a defined space is formed which, depending on the length of the inserted inner tube, defines a retention space . This retention space fills with the slowly pouring out the contents of the laboratory tube with the outflowing liquid, so that only the excess runs off and at Returning the tube to the vertical completely runs out of this space. In order to allow a complete reflux, the space must not be so narrow that capillary forces hold part of the liquid when it is reset, which results in the lower limit of about 0.5-1 mm. On the other hand, however, the gap should not become too wide either, since otherwise the length of the inner tube becomes correspondingly smaller for a certain retention volume and additional errors can occur when emptying. In addition, in the case of narrow laboratory tubes, the inner opening of the inserted inner tube used for filling and emptying becomes correspondingly small, which in turn can lead to handling disadvantages. It is therefore advantageous to expand this opening in a funnel shape or, if desired, to provide it with a pouring spout.
Die feste Verbindung zwischen Laborröhrchen und Innenrohr wird vorzugsweise dadurch bewirkt, daß das Innenrohr eine entsprechende Verdickung in seinem oberen Teil aufweist, welche mit einem gewissen Preßdruck in die Öffnung des Laborröhrchen eingeschoben werden kann. Ein angeformter Kragen kann vorgesehen sein, um ein zu tiefes Eindrücken in das Röhrchen zu verhindern. Alternativ ist es möglich, den Kragen außen um das Röhrchen herumgreifend auszubilden und mit einem Preßdruck zu halten oder mit einem Gewinde zu versehen, mittels dessen es auf ein entsprechendes Gegengewinde des Laborröhrchens aufgeschraubt werden kann. Auf die Verdickung im Inneren kann dann verzichtet werden.The firm connection between the laboratory tube and the inner tube is preferably brought about in that the inner tube has a corresponding thickening in its upper part, which can be inserted into the opening of the laboratory tube with a certain pressure. A molded collar can be provided to prevent the tube from being pressed too deeply. Alternatively, it is possible to form the collar around the outside of the tube and to hold it with a pressing pressure or to provide it with a thread by means of which it can be screwed onto a corresponding mating thread of the laboratory tube. The thickening inside can then be dispensed with.
Laborröhrchen bestehen heute praktisch ausschließlich aus Glas oder Kunststoff und lassen sich mit sehr geringen Toleranzen fertigen. Die erfindungsgemäß eingesetzten Innenröhrchen werden vorzugsweise ebenfalls aus Kunststoff gefertigt, welcher einerseits genügend Elastizität aufweist um eine dichte Verbindung mit dem Laborröhrchen einzugehen und andererseits so preiswert ist, daß die ganze Vorrichtung als Wegwerf-Artikel gefertigt und daher nach Gebrauch weggeworfen werden kann. Falls Laborröhrchen und Innenrohr aus dem gleichen Kunststoff gefertigt sind, ergibt sich zusätzlich die vorteilhafte Möglichkeit einer Recyclisierung des Kunststoffs, ohne daß die beiden Teile wieder getrennt werden müssen.Today, laboratory tubes are made almost entirely of glass or plastic and can be manufactured with very small tolerances. The inner tubes used according to the invention are preferably also made of plastic, which on the one hand has sufficient elasticity to form a tight connection with the laboratory tube and on the other hand is so inexpensive that the entire device is manufactured as a disposable article and can therefore be thrown away after use. If the laboratory tube and inner tube are made of the same plastic, there is also the advantageous possibility of recycling the plastic without the two parts must be separated again.
Die vorliegende Erfindung ist insbesondere für die handelsüblichen Zentrifugengläschen gedacht, welche einen Inhalt von etwa 15 ml aufweisen und z.B. mit Markierungen für 3, 5 und 10 ml versehen sind. Bei einer Länge von etwa 10 cm weisen solche Röhrchen einen Innendurchmesser von 14 mm auf. Ein eingeschobenes Innenröhrchen mit 1,2 mm Außendurchmesser und einer Länge von 15 mm hat ein Rückhaltevolumen von 1 cm³. Unter Verwendung der vorgegebenen Markierungen lassen sich daher sehr einfach Verdünnungs- oder Konzentrationsverhältnisse von 1:3, 1:5 oder 1:10 ohne zusätzlich Meßhilfen einstellen.The present invention is particularly intended for the commercially available centrifuge tubes, which have a content of about 15 ml and e.g. with markings for 3, 5 and 10 ml. With a length of about 10 cm, such tubes have an inner diameter of 14 mm. An inserted inner tube with an outer diameter of 1.2 mm and a length of 15 mm has a retention volume of 1 cm³. Using the specified markings, dilution or concentration ratios of 1: 3, 1: 5 or 1:10 can therefore be set very easily without additional measuring aids.
Obwohl die Vorrichtung an sich für die Konzentrierung von Sedimenten in Zentrifugengläschen entwickelt worden ist, läßt sich die gleiche Vorrichtung natürlich auch für anders geformte Gefäße verwenden, solange diese eine Öffnung besitzen, welche genau mit der Vorrichtung zusammenpaßt. Beispielsweise kann die übliche aus Kunststoff gefertigte 10 ml Spritze, die zum Zentrifugieren einen verkürzbaren Kolbenschaft besitzt, nach dem Zentrifugieren ebenfalls mit einem solchen Innenrohr versehen werden und auf diese Weise nicht nur eine definierte Menge Serum sondern auch eine definierte Menge des im Serum wieder resuspendierten Sediments erhalten werden, welches zur weiteren Untersuchung zur Verfügung steht. Auch andere Laborgefäße wie Erlenmeyer-Kolben und Rundkolben etc., welche eine zylindrische Öffnung mit geeigneten Innendurchmesser besitzen, können durch Aufsatz einer entsprechenden Rückhaltevorrichtung in ein Meßgefäß verwandelt werden. Weitere Anwendungsformen sind denkbar ohne jedoch hier im Einzelnen aufgezählt zu sein.Although the device itself has been developed for the concentration of sediments in centrifuge tubes, the same device can of course also be used for differently shaped vessels as long as they have an opening which fits exactly with the device. For example, the usual 10 ml syringe made of plastic, which has a shortenable piston shaft for centrifuging, can also be provided with such an inner tube after centrifuging and in this way not only a defined amount of serum but also a defined amount of the sediment resuspended in the serum be obtained, which is available for further investigation. Other laboratory vessels such as Erlenmeyer flasks and round-bottom flasks etc., which have a cylindrical opening with a suitable inner diameter, can also be converted into a measuring vessel by attaching a corresponding retention device. Other forms of application are conceivable without being listed in detail here.
An den beigefügten Figuren wird die Erfindung näher erläutert, ohne daß sie jedoch darauf beschränkt sein soll.The invention is explained in more detail in the accompanying figures, but is not intended to be limited thereto.
Figur 1 zeigt ein Zentrifugenglas mit eingesetztem Rückhaltesystem, wobei Figur 1a ein Schnitt durch das System ist, Figur 1b das gefüllte Röhrchen zeigt, Figur 1c das schräg gestellte Röhrchen mit der auslaufenden Flüssigkeit zeigt, Figur 1d das Röhrchen im geleerten Zustand mit dem Restvolumen in der Rückhaltevorrichtung und Figur 1e das zurückgedrehte Röhrchen mit dem Restvolumen auf dem Sediment wiedergibt. FIG. 1 shows a centrifuge glass with a retention system inserted, FIG. 1a being a section through the system, FIG. 1b showing the filled tube, FIG. 1c showing the inclined tube with the escaping liquid, FIG. 1d shows the tube in the emptied state with the remaining volume in the retaining device and FIG. 1e shows the turned-back tube with the remaining volume on the sediment.
Figur 2 ist eine vergrößerte Wiedergabe der Figur 1e mit dem Laborröhrchen 1, dem eingesetzten Innenröhrchen 2 welches eine Verdickung 3 aufweist, die in den Innendurchmesser des Laborröhrchens 1 hineinpaßt und einen Kragen 4, welcher das Laborröhrchen 1 außen umschließt. In die Verdickung 3 und den Kragen 4 ist eine trichterförmige Erweiterung 5 eingearbeitet. Der Zwischenraum zwischen dem Innenröhrchen 2 und dem Laborröhrchen 1 definiert das Rückhaltevolumen 7, welches dem Volumen 7a in zurückgestelltem Zustand entspricht. Ein Sediment 8 ist am Boden des Röhrchens 2 angedeutet. Ferner sind 2 Füllstandsmarkierungen 6 angegeben, die z.B. einer Füllmenge von 3 cm³ entsprechen. Figure 2 is an enlarged representation of Figure 1e with the laboratory tube 1, the inserted
Figur 3 zeigt eine vereinfachte Ausführungsform des Innenrohrs, welche lediglich aus dem Innenrohr 2 und der angeformten Verdickung 3 besteht. Figure 3 shows a simplified embodiment of the inner tube, which consists only of the
Figur 4 zeigt eine Vorrichtung, welche außer dem Innenrohr 2, der Verdickung 3 und dem Kragen 4 noch einen angeformten Gießschnabel 9 aufweist. FIG. 4 shows a device which, in addition to the
Figur 5 zeigt eine handelsübliche Spritze mit dem Außenrohr 10, dem Kolben 11, dem mit mehreren Bruchkerben 12 versehenen Handgriff 13 sowie einer aufschraubbaren Verschlußkappe 14 an die der Kanülenansatz 15 angeformt ist. Figur 5a zeigt die gleiche Spritze mit dem erfindungsgemäßen Aufsatz, der über eine Schraubverbindung 16 auf die Spritze aufgeschraubt ist. Der Handgriff 13 ist an der ersten Bruchkerbe 12 abgebrochen. FIG. 5 shows a commercially available syringe with the
- 11
- LaborröhrchenLaboratory tubes
- 22nd
- InnenröhrchenInner tube
- 33rd
- Verdickungthickening
- 44th
- Kragencollar
- 55
- Einfüllöffnung (trichterförmig erweitert)Filler opening (funnel-shaped)
- 66
- FüllmarkierungFill mark
- 77
- RückhaltevolumenRetention volume
- 7a7a
- Rückhaltevolumen nach RücklaufRetention volume after return
- 88th
- Sedimentsediment
- 99
- GießschnabelPouring beak
- 1010th
- AußenrohrOuter tube
- 1111
- Kolbenpiston
- 1212th
- BruchkerbeScore
- 1313
- HandgriffHandle
- 1414
- VerschlußkappeSealing cap
- 1515
- KanülenansatzCannula hub
- 1616
- SchraubverbindungScrew connection
Claims (4)
- Laboratory test tube for the dosing of liquids, whereby the laboratory test tube (1) possesses markings (6) for the filling with the liquid, with which opening of the laboratory test tube (1) is connected a shortened inner tube (2), whereby, between laboratory test tube (1) and inner tube (2), is present a space with constant retention volume (7) in the case of the pouring out of the tube content, characterised in that the intermediate space has a width of at least 0.5 to maximum 1 mm.
- Laboratory test tube according to claim 1, characterised in that laboratory test tube (1) and inner tube (2) consist of recyclable synthetic material which are connected via a press connection.
- Laboratory test tube according to claim 1 or 2, characterised in that the inner tube (2) consists of a cylindrical tube, by the length and outer diameter of which and the inner diameter of the laboratory test tube (1) the retention volume (7) is defined, the inner tube (2) has on its upper end a thickening (3), the outer diameter of which corresponds to the inner diameter of the laboratory test tube (1), the inner tube (2) has a collar (4) which encompasses the opening of the laboratory test tube (1) and collar (4) and thickening (3) comprise a funnel-shaped filling opening (5).
- Use of laboratory test tubes according to one of claims 1 to 3 for the resuspension of the sediments obtained by centrifuging.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE9203973U DE9203973U1 (en) | 1992-03-25 | 1992-03-25 | Laboratory tubes for dosing liquids |
| DE9203973U | 1992-03-25 | ||
| DE4243478A DE4243478A1 (en) | 1992-03-25 | 1992-12-22 | Laboratory tubes for dosing liquids |
| DE4243478 | 1992-12-22 | ||
| PCT/EP1993/000615 WO1993018858A1 (en) | 1992-03-25 | 1993-03-16 | Liquid-dosing tube_________________________________________ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP0632749A1 EP0632749A1 (en) | 1995-01-11 |
| EP0632749B1 true EP0632749B1 (en) | 1995-10-04 |
Family
ID=25921616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP93920526A Expired - Lifetime EP0632749B1 (en) | 1992-03-25 | 1993-03-16 | Liquid-dosing tube |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0632749B1 (en) |
| AT (1) | ATE128645T1 (en) |
| AU (1) | AU3749093A (en) |
| CA (1) | CA2117696A1 (en) |
| DE (2) | DE4243478A1 (en) |
| WO (1) | WO1993018858A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103055988A (en) * | 2012-08-27 | 2013-04-24 | 浙江硕华医用塑料有限公司 | Centrifugal tube |
| NL2014739B1 (en) * | 2015-04-30 | 2017-01-18 | Labonovum B V | Fluid collection device. |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL252802A (en) * | 1959-11-20 | |||
| US3718133A (en) * | 1971-01-12 | 1973-02-27 | Damon Corp | Container unit for liquid samples |
| US3748099A (en) * | 1971-09-07 | 1973-07-24 | Toluca Ind Corp | Liquid handling device |
| US4342724A (en) * | 1980-08-21 | 1982-08-03 | E. R. Squibb & Sons, Inc. | Red cell labeling vial |
-
1992
- 1992-12-22 DE DE4243478A patent/DE4243478A1/en not_active Withdrawn
-
1993
- 1993-03-16 EP EP93920526A patent/EP0632749B1/en not_active Expired - Lifetime
- 1993-03-16 WO PCT/EP1993/000615 patent/WO1993018858A1/en active IP Right Grant
- 1993-03-16 CA CA002117696A patent/CA2117696A1/en not_active Abandoned
- 1993-03-16 AT AT93920526T patent/ATE128645T1/en not_active IP Right Cessation
- 1993-03-16 DE DE59300720T patent/DE59300720D1/en not_active Expired - Lifetime
- 1993-03-16 AU AU37490/93A patent/AU3749093A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU3749093A (en) | 1993-10-21 |
| DE4243478A1 (en) | 1993-09-30 |
| DE59300720D1 (en) | 1995-11-09 |
| EP0632749A1 (en) | 1995-01-11 |
| WO1993018858A1 (en) | 1993-09-30 |
| ATE128645T1 (en) | 1995-10-15 |
| CA2117696A1 (en) | 1993-09-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69413157T2 (en) | Collection tube | |
| DE60306232T2 (en) | Cotton ball device and procedure | |
| DE60212522T2 (en) | Device for taking liquid samples | |
| EP0749759A1 (en) | Device for dispensing a fluid from a receptacle | |
| DE1598301A1 (en) | Sediment container for centrifuges and cap for such containers | |
| DE2625876B2 (en) | Device for preparing liquid samples for examination and analysis | |
| EP1474235A1 (en) | Sample preparation device and test device set based thereon | |
| EP0722083B1 (en) | Method and device for sampling and smearing liquids | |
| DE2638743A1 (en) | DEVICE FOR DISPENSING BIOLOGICAL LIQUID | |
| WO1998022218A1 (en) | Sample vessel for taking blood samples | |
| DE69528865T2 (en) | collection tubes | |
| DE3853803T2 (en) | Self-drip catcher. | |
| EP0331912A2 (en) | Sample receiving element able to be filled for handling liquid samples | |
| DE3415580C2 (en) | ||
| US5725832A (en) | Laboratory test tubes for the dosing of liquids | |
| EP0632749B1 (en) | Liquid-dosing tube | |
| DE69914841T2 (en) | Method and device for taking samples from a test tube | |
| EP1068013A2 (en) | Device for receiving and discharging a given amount of liquid | |
| CH615129A5 (en) | ||
| DE3618558C2 (en) | ||
| EP0907414B1 (en) | Method for achieving chemical, in particular biochemical reactions and pipette tip with reaction vessel | |
| DE2637273A1 (en) | Blood fraction filtering device - with riser pipe surmounting hole in jacket pipe partition with filtering piston cup | |
| DE3202232C2 (en) | Removal insert for pharmaceutical containers, in particular pharmaceutical bottles | |
| DE9203973U1 (en) | Laboratory tubes for dosing liquids | |
| EP0343534B1 (en) | Container for preserving liquids, in particular bodily liquids |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
| 17P | Request for examination filed |
Effective date: 19940902 |
|
| AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH DE ES FR GB IT LI LU NL SE |
|
| 17Q | First examination report despatched |
Effective date: 19950224 |
|
| GRAA | (expected) grant |
Free format text: ORIGINAL CODE: 0009210 |
|
| AK | Designated contracting states |
Kind code of ref document: B1 Designated state(s): AT BE CH DE ES FR GB IT LI LU NL SE |
|
| PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: ES Free format text: THE PATENT HAS BEEN ANNULLED BY A DECISION OF A NATIONAL AUTHORITY Effective date: 19951004 |
|
| REF | Corresponds to: |
Ref document number: 128645 Country of ref document: AT Date of ref document: 19951015 Kind code of ref document: T |
|
| ITF | It: translation for a ep patent filed | ||
| REF | Corresponds to: |
Ref document number: 59300720 Country of ref document: DE Date of ref document: 19951109 |
|
| GBT | Gb: translation of ep patent filed (gb section 77(6)(a)/1977) |
Effective date: 19951016 |
|
| PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: SE Effective date: 19960104 |
|
| ET | Fr: translation filed | ||
| PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: LU Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 19960331 |
|
| PLBE | No opposition filed within time limit |
Free format text: ORIGINAL CODE: 0009261 |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT |
|
| 26N | No opposition filed | ||
| PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: GB Payment date: 20001227 Year of fee payment: 9 |
|
| PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: CH Payment date: 20010423 Year of fee payment: 9 |
|
| REG | Reference to a national code |
Ref country code: GB Ref legal event code: IF02 |
|
| PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: GB Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20020316 |
|
| PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: LI Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20020331 Ref country code: CH Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20020331 |
|
| GBPC | Gb: european patent ceased through non-payment of renewal fee |
Effective date: 20020316 |
|
| REG | Reference to a national code |
Ref country code: CH Ref legal event code: PL |
|
| PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: NL Payment date: 20060320 Year of fee payment: 14 |
|
| PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: FR Payment date: 20060322 Year of fee payment: 14 |
|
| PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: BE Payment date: 20060323 Year of fee payment: 14 |
|
| PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: AT Payment date: 20060324 Year of fee payment: 14 |
|
| PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: IT Payment date: 20060331 Year of fee payment: 14 |
|
| PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: AT Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20070316 |
|
| NLV4 | Nl: lapsed or anulled due to non-payment of the annual fee |
Effective date: 20071001 |
|
| BERE | Be: lapsed |
Owner name: *GUNDELSHEIMER PETER Effective date: 20070331 |
|
| PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: BE Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20070331 |
|
| REG | Reference to a national code |
Ref country code: FR Ref legal event code: ST Effective date: 20071130 |
|
| PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: NL Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20071001 |
|
| PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: FR Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20070402 |
|
| PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: IT Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20070316 |
|
| PGFP | Annual fee paid to national office [announced via postgrant information from national office to epo] |
Ref country code: DE Payment date: 20110303 Year of fee payment: 19 |
|
| REG | Reference to a national code |
Ref country code: DE Ref legal event code: R119 Ref document number: 59300720 Country of ref document: DE Effective date: 20121002 |
|
| PG25 | Lapsed in a contracting state [announced via postgrant information from national office to epo] |
Ref country code: DE Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20121002 |