EP0563239A1 - A truncated interleukin-1 receptor gene for the treatment of arthritis - Google Patents
A truncated interleukin-1 receptor gene for the treatment of arthritisInfo
- Publication number
- EP0563239A1 EP0563239A1 EP92902630A EP92902630A EP0563239A1 EP 0563239 A1 EP0563239 A1 EP 0563239A1 EP 92902630 A EP92902630 A EP 92902630A EP 92902630 A EP92902630 A EP 92902630A EP 0563239 A1 EP0563239 A1 EP 0563239A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- interleukin
- gene
- receptor
- cell line
- synovial cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
- C12N2740/13041—Use of virus, viral particle or viral elements as a vector
- C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
- C12N2740/13041—Use of virus, viral particle or viral elements as a vector
- C12N2740/13045—Special targeting system for viral vectors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Public Health (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Rheumatology (AREA)
- Veterinary Medicine (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Toxicology (AREA)
- Pain & Pain Management (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La présente invention se rapporte à un procédé d'utilisation in vivo d'un gène codant un domaine de liaison extracellulaire d'interleukine-1 d'un récepteur d'interleukine-1 susceptible de se lier à l'interleukine-1 et de la neutraliser, lequel procédé consiste à utiliser des techniques de recombinaision pour produire une lignée cellulaire placée sous le contrôle d'un promoteur ecaryote approprié comprenant le gène codant pour le domaine de liaison extracellulaie d'interleukine-1 du récepteur d'interleukine-1; et à amorcer la transfection d'ADN du gène au moyen de l'introduction de particules virales obtenues de la lignée cellulaire directement dans des cellules synoviales recouvrant un espace d'articulation d'un hôte mammifère. Selon une variante, des cellules synoviales provenant de l'articulation du patient peuvent être échangées par transduction avec le vecteur rétroviral portant le gène thérapeutique et un marqueur choisi pour la sélection de cellules de transduction uniquement, et les cellules devenues autologues d'un point de vue thérapeutique peuvent être réintroduites dans l'articulation par une transplantation. En outre, on décrit un procédé de préparation d'un gène codant un domaine de liaison extracellulaire d'interleukine-1 d'un récepteur d'interleukine-1 et qui est susceptible de se lier à l'interleukine-1 et de la neutraliser. Un composé destiné à une administration parentérale en doses prophylactiques ou thérapeutiques à un patient, et contenant le gène de l'invention et un véhicule pharmaceutiquement approprié est aussi décrit.The present invention relates to a method for the in vivo use of a gene encoding an extracellular binding domain of interleukin-1 of an interleukin-1 receptor capable of binding to interleukin-1 and of the neutralize, which method involves using recombination techniques to produce a cell line under the control of an appropriate ecaryotic promoter comprising the gene encoding the interleukin-1 extracellular binding domain of the interleukin-1 receptor; and initiating DNA transfection of the gene by introducing viral particles obtained from the cell line directly into synovial cells covering a joint space of a mammalian host. Alternatively, synovial cells from the patient's joint can be exchanged by transduction with the retroviral vector carrying the therapeutic gene and a marker chosen for the selection of transduction cells only, and cells that have become autologous from a point of therapeutic view can be reintroduced into the joint through a transplant. In addition, a method of preparing a gene encoding an interleukin-1 extracellular binding domain of an interleukin-1 receptor and which is capable of binding and neutralizing interleukin-1 is described. . A compound intended for parenteral administration in prophylactic or therapeutic doses to a patient, and containing the gene of the invention and a pharmaceutically suitable vehicle is also described.
Description
Claims
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US63098190A | 1990-12-20 | 1990-12-20 | |
US630981 | 1990-12-20 | ||
PCT/US1991/009231 WO1992011359A1 (en) | 1990-12-20 | 1991-12-09 | A truncated interleukin-1 receptor gene for the treatment of arthritis |
Publications (2)
Publication Number | Publication Date |
---|---|
EP0563239A1 true EP0563239A1 (en) | 1993-10-06 |
EP0563239A4 EP0563239A4 (en) | 1994-10-12 |
Family
ID=24529337
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP19920902630 Withdrawn EP0563239A4 (en) | 1990-12-20 | 1991-12-09 | A truncated interleukin-1 receptor gene for the treatment of arthritis. |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0563239A4 (en) |
JP (1) | JPH06504440A (en) |
CA (1) | CA2098848A1 (en) |
WO (1) | WO1992011359A1 (en) |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6228356B1 (en) | 1990-12-20 | 2001-05-08 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Viral vectors to inhibit leukocyte infiltration or cartilage degradation of joints |
US6156304A (en) * | 1990-12-20 | 2000-12-05 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Gene transfer for studying and treating a connective tissue of a mammalian host |
US5858355A (en) * | 1990-12-20 | 1999-01-12 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | IRAP gene as treatment for arthritis |
US6413511B1 (en) * | 1990-12-20 | 2002-07-02 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Cartilage alterations by administering to joints chondrocytes comprising a heterologous polynucleotide |
US6159464A (en) * | 1990-12-20 | 2000-12-12 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Viral vectors to inhibit leukocyte infiltration or cartilage degradation of joints |
US5792751A (en) * | 1992-04-13 | 1998-08-11 | Baylor College Of Medicine | Tranformation of cells associated with fluid spaces |
JPH07508988A (en) * | 1992-07-13 | 1995-10-05 | ベイラー・カレッジ・オブ・メディシン | Targeting somatic gene therapy to joints |
EP0701563A4 (en) * | 1993-03-08 | 1997-07-02 | Univ Pittsburgh | Gene transfer for treating a connective tissue of a mammalian host |
US5645829A (en) * | 1993-06-18 | 1997-07-08 | Beth Israel Hospital Association | Mesothelial cell gene therapy |
AU7516594A (en) * | 1993-07-28 | 1995-02-28 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Pre-binding of retroviral vector particles with complement components to enable the performance of human gene therapy (in vivo) |
EP0690673A4 (en) * | 1993-12-14 | 1996-05-29 | Univ Pittsburgh | Systemic gene treatment of connective tissue diseases |
JPH083058A (en) * | 1994-06-17 | 1996-01-09 | Hoechst Japan Ltd | New medicine for bone metabolic disease |
DK0807183T3 (en) * | 1994-08-26 | 2001-03-05 | Aventis Pharma Gmbh | Gene therapy of diseases caused by the immune system by a cell-specific active substance regulated by cellec |
US6096728A (en) * | 1996-02-09 | 2000-08-01 | Amgen Inc. | Composition and method for treating inflammatory diseases |
US6294170B1 (en) | 1997-08-08 | 2001-09-25 | Amgen Inc. | Composition and method for treating inflammatory diseases |
JP2001514235A (en) * | 1997-09-02 | 2001-09-11 | カイロン コーポレイション | Compositions and methods for treating arthritis utilizing gene therapy |
EP1939300A1 (en) | 1999-05-28 | 2008-07-02 | Targeted Genetics Corporation | Methods and compositions for lowering the level of tumor necrosis factor (TNF) in TNF-associated disorders |
US6537540B1 (en) | 1999-05-28 | 2003-03-25 | Targeted Genetics Corporation | Methods and composition for lowering the level of tumor necrosis factor (TNF) in TNF-associated disorders |
WO2001013960A1 (en) * | 1999-08-20 | 2001-03-01 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Methods for in vivo gene delivery to sites of cartilage damage |
WO2002083080A2 (en) * | 2001-04-17 | 2002-10-24 | Genetix Pharmaceuticals, Inc. | Method of treating arthritis using lentiviral vectors in gene therapy |
US7619066B2 (en) | 2004-04-02 | 2009-11-17 | Amgen Inc. | IL-1ra variants |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0318296A2 (en) * | 1987-11-25 | 1989-05-31 | Immunex Corporation | Interleukin-1 receptors |
WO1989004838A1 (en) * | 1987-11-25 | 1989-06-01 | Immunex Corporation | Interleukin-1 receptors |
-
1991
- 1991-12-09 JP JP4502854A patent/JPH06504440A/en active Pending
- 1991-12-09 WO PCT/US1991/009231 patent/WO1992011359A1/en not_active Application Discontinuation
- 1991-12-09 CA CA002098848A patent/CA2098848A1/en not_active Abandoned
- 1991-12-09 EP EP19920902630 patent/EP0563239A4/en not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0318296A2 (en) * | 1987-11-25 | 1989-05-31 | Immunex Corporation | Interleukin-1 receptors |
WO1989004838A1 (en) * | 1987-11-25 | 1989-06-01 | Immunex Corporation | Interleukin-1 receptors |
Non-Patent Citations (5)
Title |
---|
BRAIN RESEARCH BULLETIN vol. 24, no. 6 , 1990 pages 787 - 792 M. TSUDA ET AL. 'Retrovirus-mediated gene transfer into mouse cerebellar primary culture and its application to the neural transplantation.' * |
NATURE vol. 320 , 20 March 1986 , LONDON, GB pages 213 - 214 M. ROBERTSON 'Gene therapy. Desperate appliances.' * |
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA vol. 86, no. 23 , December 1989 , WASHINGTON, DC, USA pages 9079 - 9083 P. ROUX ET AL. 'A versatile and potentially general approach to the targeting of specific cell types by retroviruses: Application to the infection of human cells by means of major histocompatibility complex class I and class II antigens by mouse ecotropic murine leukemia virus-derived viruses.' * |
SCIENCE vol. 244, no. 4910 , 16 June 1989 , WASHINGTON, DC, USA pages 1275 - 1281 T. FRIEDMANN 'Progress toward human gene therapy.' * |
See also references of WO9211359A1 * |
Also Published As
Publication number | Publication date |
---|---|
WO1992011359A1 (en) | 1992-07-09 |
EP0563239A4 (en) | 1994-10-12 |
JPH06504440A (en) | 1994-05-26 |
CA2098848A1 (en) | 1992-06-21 |
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Legal Events
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PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
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Effective date: 19930715 |
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17Q | First examination report despatched |
Effective date: 19980623 |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: EXAMINATION IS IN PROGRESS |
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18D | Application deemed to be withdrawn |
Effective date: 20010911 |