EP0548178A1 - Quantitative analyse und überwachung der proteinstruktur durch subtraktive chromatographie - Google Patents

Quantitative analyse und überwachung der proteinstruktur durch subtraktive chromatographie

Info

Publication number
EP0548178A1
EP0548178A1 EP19910916341 EP91916341A EP0548178A1 EP 0548178 A1 EP0548178 A1 EP 0548178A1 EP 19910916341 EP19910916341 EP 19910916341 EP 91916341 A EP91916341 A EP 91916341A EP 0548178 A1 EP0548178 A1 EP 0548178A1
Authority
EP
European Patent Office
Prior art keywords
matrix
concentration
sample
protein
binding sites
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19910916341
Other languages
English (en)
French (fr)
Inventor
Noubar B. Afeyan
Fred E. Regnier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
PerSeptive Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PerSeptive Biosystems Inc filed Critical PerSeptive Biosystems Inc
Publication of EP0548178A1 publication Critical patent/EP0548178A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N2030/626—Detectors specially adapted therefor calibration, baseline
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Definitions

  • FIGS. 7 and 8 are chromatograms generated using the analysis technique of the present invention for measuring the concentration of Immunoglobulin in solution using a Protein A column where BSA is a contaminant;
  • a preferred aspect of the present invention involves high speed assays, e.g., less than 10 seconds.
  • the above discussed analyses can be performed in periods substantially shorter than one minute, often shorter than 30 seconds, and frequently less than 10 seconds, if one employs a small volume column containing a matrix medium of the type described below.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
EP19910916341 1990-08-10 1991-08-01 Quantitative analyse und überwachung der proteinstruktur durch subtraktive chromatographie Withdrawn EP0548178A1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56612190A 1990-08-10 1990-08-10
US566121 1990-08-10

Publications (1)

Publication Number Publication Date
EP0548178A1 true EP0548178A1 (de) 1993-06-30

Family

ID=24261583

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19910916341 Withdrawn EP0548178A1 (de) 1990-08-10 1991-08-01 Quantitative analyse und überwachung der proteinstruktur durch subtraktive chromatographie

Country Status (5)

Country Link
EP (1) EP0548178A1 (de)
JP (1) JPH06500396A (de)
AU (1) AU654503B2 (de)
CA (1) CA2088360A1 (de)
WO (1) WO1992002815A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3175941B2 (ja) * 1991-03-28 2001-06-11 パーセプティブ バイオシステムズ インコーポレイテッド クロマトグラフ溶出液の減法に依るオンライン生成物確認方法及び装置
CA2240325A1 (en) 1998-03-27 1998-11-14 Synsorb Biotech, Inc. Methods for screening compound libraries
US6720190B1 (en) 1998-03-27 2004-04-13 Ole Hindsgaul Methods for screening compound libraries
US6613575B1 (en) 1998-03-27 2003-09-02 Ole Hindsgaul Methods for screening compound libraries
US6054047A (en) 1998-03-27 2000-04-25 Synsorb Biotech, Inc. Apparatus for screening compound libraries
DE19945351C2 (de) * 1999-09-22 2002-04-18 Lohmann Therapie Syst Lts Verfahren zum Auffinden und zur Isolierung pharmakologisch wirksamer Verbindungen aus Substanzgemischen
EP3702775A1 (de) * 2009-06-26 2020-09-02 Cytiva Sweden AB Verfahren zur bestimmung von bindungskapazitäten in einem chromatographiesystem
SG11202103572VA (en) * 2018-10-09 2021-05-28 C Technologies Inc Mass control system for chromatography
CN114280208B (zh) * 2021-11-03 2024-05-24 鼎康(武汉)生物医药有限公司 一种亲和层析填料动态载量的测定方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4204424A (en) * 1979-01-08 1980-05-27 Phillips Petroleum Company Chromatographic analyzer detector gain adjustment
JPS56114761A (en) * 1980-02-14 1981-09-09 Shinotesuto Kenkyusho:Kk Fractional determination method of zinc in fresh blood serum by affinity chromatography
EP0141259B1 (de) * 1981-03-09 1989-06-07 Environmental Sciences Associates, Inc. Flüssigkeitschromatographie
JPS58216954A (ja) * 1982-06-11 1983-12-16 Shimadzu Corp 低温高速液体クロマトグラフイ
JPS5984828A (ja) * 1982-11-08 1984-05-16 Suntory Ltd 安定化免疫複合体およびその利用方法
IT1150598B (it) * 1982-12-14 1986-12-17 Ines Bianchi Procedimento per la purificazione della superossidodismutasi mediante cromatografia di affinita'
JPS59143958A (ja) * 1983-02-07 1984-08-17 Sekisui Chem Co Ltd フイブロネクチンの定量法
JPS63236964A (ja) * 1987-03-26 1988-10-03 Ube Ind Ltd 糖尿病の診断法
AU4800090A (en) * 1988-12-05 1990-06-26 Primus Corporation Method for determination of glycated proteinaceous species

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9202815A2 *

Also Published As

Publication number Publication date
JPH06500396A (ja) 1994-01-13
WO1992002815A2 (en) 1992-02-20
AU8530691A (en) 1992-03-02
CA2088360A1 (en) 1992-02-11
AU654503B2 (en) 1994-11-10
WO1992002815A3 (en) 1992-04-30

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