EP0339710B1 - Thermostatic device for sample supports - Google Patents

Thermostatic device for sample supports Download PDF

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Publication number
EP0339710B1
EP0339710B1 EP89200936A EP89200936A EP0339710B1 EP 0339710 B1 EP0339710 B1 EP 0339710B1 EP 89200936 A EP89200936 A EP 89200936A EP 89200936 A EP89200936 A EP 89200936A EP 0339710 B1 EP0339710 B1 EP 0339710B1
Authority
EP
European Patent Office
Prior art keywords
sample support
receptacles
drains
plate
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP89200936A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP0339710A2 (en
EP0339710A3 (en
Inventor
Hendrikus J. Mansveld Beck
Hans Van Dijk
Henk Versteeg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rijksuniversiteit Utrecht
Original Assignee
Rijksuniversiteit Utrecht
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rijksuniversiteit Utrecht filed Critical Rijksuniversiteit Utrecht
Priority to AT89200936T priority Critical patent/ATE79307T1/de
Publication of EP0339710A2 publication Critical patent/EP0339710A2/en
Publication of EP0339710A3 publication Critical patent/EP0339710A3/en
Application granted granted Critical
Publication of EP0339710B1 publication Critical patent/EP0339710B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/02—Water baths; Sand baths; Air baths

Definitions

  • the invention relates to a device with one or more receptacles adapted to receive a sample support having a plate with a plurality of downwardly directed recesses, such as a so-called microtiter plate, which device is provided with means for maintaining a heat carrying liquid in a liquid store on a controlled temperature and duct means connected between the store and the receptacles.
  • the invention aims to provide a device with which the samples can be brought to temperature in a considerable faster way, so that also the moment at which the desired temperature is attained is determined more accurately.
  • the receptacles and/or the sample support are provided with sealing means to form a sealed compartment between the receptacle and the plate of the sample support and that the duct means contain a connection from the liquid store to the said compartment and a drain from said compartment to the liquid store, at least one of the connections and the drain being provided with closable valve means.
  • US-A-3 505 023 shows a sample support having an upper surface with downwardly extending depressions for receiving samples, and a temperature conditioning fluid chamber below said surface, which chamber is provided with a fluid inlet and outlet. Connecting these in- and outlets to a hose or suchlike duct is rather cumbersome and leads to complicated connection structures in case of a considerable number of sample supports. Further bringing the samples into the depressions has to be done in situations, in which the inlet and the outlet impede somewhat the free acces to the depressions, whereas emptying the chamber seems desirable in case quick heating to the desired temperature is necessary.
  • microtiter plates in a device for heating samples for microbiological purposes is known from the European Patent Application EPA-A-0 063 744, in which the microtiter plate is placed on a plate which is cooled by means of a liquid and the heat given off by an electromotor is dissipated by means of a blower.
  • the receptacles have a surface with a border, which forms a seal with an edge of the sample support.
  • a regular flow with which it is attained in a high degree that different cells of the microtiter plate are exposed to equal temperature control conditions, is enhanced when the connection to the compartment is made at the central region of the sample support.
  • one has preferably a plurality of drains near the border of the sample support.
  • the air can be driven out of the compartments when maintaining a regular flow, which in an equal measure serves the cells of the compartments, it is according to a further elaboration of the invention provided that there are lower drains joining the bottom of the compartments and upper drains having their opening above the bottom and near to the lower surface of the plate of a related sample support, wherewith it is particularly advantageous if the sample supports tilt slightly in the compartments located below them.
  • reference 1 indicates a reservoir. Above the left side of this reservoir six receptacles 2 have been applied and above the smaller right side a plate 3 is present with an intermediate wall 4 suspending downwardly, which, however, does not reach the bottom 5 (see fig. 2) of the reservoir. In the left part of the reservoir a plate 7 lies on two support beams 6, which plate tilts upwardly from the sides towards the centre with an angle of about 15°.
  • the tube 10 is connected to the output of a valve 13, which by means of a feeding tube 14 is connected to a main duct 15 which is located below the liquid level 16 in the reservoir 1.
  • the main duct 15 is U-shaped and has a feed 17 from a pump and thermostatic controlled temperature adjustment or thermostate device 18, which may be of a type known per se. At the ends of the U-shaped tube 15 flow resistances 19 have been mounted.
  • Each cushion 8 of elastic material preferably elastomeric foam material with closed cells, is provided with drains 23 located at the lower side of each cushion and 24 at the higher side.
  • the drains 24 have upwardly emerging small tubes, so that their mouthes are located above the surface of the cushion 8.
  • microtiter plate 25 On each cushion a microtiter plate 25 is laid, which, when pressed down, with its edge 26 seals on the related cushion 8.
  • each microtiter plate 25 On each microtiter plate 25 a cover 27 is located, which with its edge 28 is pressed against the border 26 of the microtiter plate.
  • the brace 22 is mounted somewhat excentrically on pivots 21, by reason of which it passes a dead point when turning on these pivots and consequently fixes the microtiter plate 25 and the cover 27 with a snap.
  • a handle 29 is connected, which can work on a control pin 30′ of a valve 13.
  • the pin 30′ of the valve 13 is fixedly connected to a valve member 30, having closed end surfaces 31 and 32 and there between a central bore, which by means of each time four holes 34,35 and 36 is connected to the outside of the valve member 30.
  • a valve member 30 having closed end surfaces 31 and 32 and there between a central bore, which by means of each time four holes 34,35 and 36 is connected to the outside of the valve member 30.
  • an O-ring 37 is mounted and at the upper side an O-ring 38, which can cooperate with a seat 39 and 40 respectively.
  • the shown valve has the advantage, that when the counter pressure of the liquid has been overcome practically no more resistance force is encountered, so that some type of snapping action is obtained. Moreover the valve member 30 is amply mounted in the opening 44 in the sheath 43, so that no friction will occur.
  • the invention has the advantage, that when a microtiter plate has been attached to a cushion 8 and the clamps are fixed the feed of the liquid on the right temperature starts immediately, by reason of which the samples, which are located in depressions are submitted to flowing around of thermostated liquid and attain quickly the right temperature.
  • the invention combines therewith an easy applicability of the microtiter plates in the device with the possibility to shield the samples by means of the covers and to bring them quickly to the desired temperature without occurrence of locally higher temperatures, which could damage microbiological, enzymological and/or immunological processes to be studied or generated.
  • the invention has been depicted on hand of a device in which the liquid in the compartments is pressurized a little, so that the microtiter plates have to be fixed by mechanical means, it also is possible to cause the liquid flow through the compartments by means of suction, so that the sample supports are pressed down on the cushions by reason of sub pressure.

Landscapes

  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Supplying Of Containers To The Packaging Station (AREA)
  • Fats And Perfumes (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
  • Investigating Strength Of Materials By Application Of Mechanical Stress (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Signal Processing For Digital Recording And Reproducing (AREA)
  • Optical Measuring Cells (AREA)
EP89200936A 1988-04-29 1989-04-14 Thermostatic device for sample supports Expired - Lifetime EP0339710B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT89200936T ATE79307T1 (de) 1988-04-29 1989-04-14 Thermostatische vorrichtung fuer probenhalter.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL8801127A NL8801127A (nl) 1988-04-29 1988-04-29 Inrichting voor het op een bepaalde temperatuur brengen en houden van een monsterhouder.
NL8801127 1988-04-29

Publications (3)

Publication Number Publication Date
EP0339710A2 EP0339710A2 (en) 1989-11-02
EP0339710A3 EP0339710A3 (en) 1990-05-30
EP0339710B1 true EP0339710B1 (en) 1992-08-12

Family

ID=19852229

Family Applications (1)

Application Number Title Priority Date Filing Date
EP89200936A Expired - Lifetime EP0339710B1 (en) 1988-04-29 1989-04-14 Thermostatic device for sample supports

Country Status (9)

Country Link
EP (1) EP0339710B1 (fi)
JP (1) JPH0216430A (fi)
AT (1) ATE79307T1 (fi)
DE (1) DE68902414T2 (fi)
DK (1) DK208589A (fi)
ES (1) ES2035523T3 (fi)
FI (1) FI891976A (fi)
NL (1) NL8801127A (fi)
NO (1) NO891796L (fi)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2678782B1 (fr) * 1991-07-04 1995-06-30 Jaeger Dispositif de connexion entre un circuit souple electrique et un second circuit.
DE19521947C1 (de) * 1995-06-16 1996-08-22 Medipro Medizinische Diagnosti Wasserbadschüttler zur Durchführung chemischer oder biochemischer Untersuchungen
PT895813E (pt) 1997-08-08 2004-11-30 Aventis Pharma Gmbh Robot de pipetacao com dispositivo de regulacao melorada de temperatura
DE29714206U1 (de) * 1997-08-08 1997-12-11 Hoechst Marion Roussel Deutschland GmbH, 65929 Frankfurt Pipettierroboter mit verbesserter Temperiervorrichtung
DE10046224A1 (de) * 2000-09-19 2002-03-28 Merck Patent Gmbh Inkubationsvorrichtung
DE102009015869B4 (de) 2009-04-01 2011-03-03 Schneckenburger, Herbert, Prof. Dr. Mikrotiterplatte mit Heizeinrichtung
CN101632948B (zh) * 2009-09-08 2011-06-15 苏州培英实验设备有限公司 一种tshy-10梯度水浴恒温振荡器
CN110115963B (zh) * 2019-04-18 2020-12-22 通用生物系统（安徽）有限公司 一种引物合成用偶联活化剂的制备方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3505023A (en) * 1967-11-13 1970-04-07 Research Corp Thermally conditioned spot plate
US3634651A (en) * 1970-12-04 1972-01-11 Becton Dickinson Co Serological incubator
DE3115745C2 (de) * 1981-04-18 1983-03-17 Kernforschungsanlage Jülich GmbH, 5170 Jülich Verfahren und Vorrichtung zur Serienkultivierung von Mikroorganismen
JPS58154661A (ja) * 1981-10-20 1983-09-14 Toshiba Corp 自動生化学分折装置における恒温槽
JPS5930038A (ja) * 1982-08-13 1984-02-17 Toshiba Corp 恒温装置
US4775003A (en) * 1987-03-27 1988-10-04 Koh John K Laboratory materials and apparatus support and laminar flow thermal transfer device

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN, vol. 7, no. 278 (P-242)(1423), December 10, 1983; & JP-A-58 154 661 (TOKYO SHIBAURA DENKI K.K.) 14-09-1983 *
Patent Abstracts of Japan, vol. 8, no. 125(P-279)(1562), June 12, 1984; & JP-A-59 30 038 (TOKYO SHIBAURA DENKI K.K.) 17-2-1984 *

Also Published As

Publication number Publication date
EP0339710A2 (en) 1989-11-02
DE68902414D1 (de) 1992-09-17
JPH0216430A (ja) 1990-01-19
ES2035523T3 (es) 1993-04-16
NO891796L (no) 1989-10-30
FI891976A (fi) 1989-10-30
FI891976A0 (fi) 1989-04-26
DE68902414T2 (de) 1993-03-25
DK208589D0 (da) 1989-04-28
NO891796D0 (no) 1989-04-28
EP0339710A3 (en) 1990-05-30
ATE79307T1 (de) 1992-08-15
NL8801127A (nl) 1989-11-16
DK208589A (da) 1989-10-30

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