EP0307774A3 - Process for purifying plasminogen activator - Google Patents

Process for purifying plasminogen activator Download PDF

Info

Publication number
EP0307774A3
EP0307774A3 EP88114583A EP88114583A EP0307774A3 EP 0307774 A3 EP0307774 A3 EP 0307774A3 EP 88114583 A EP88114583 A EP 88114583A EP 88114583 A EP88114583 A EP 88114583A EP 0307774 A3 EP0307774 A3 EP 0307774A3
Authority
EP
European Patent Office
Prior art keywords
plasminogen activator
fibrin
washing
purifying plasminogen
purifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP88114583A
Other languages
German (de)
French (fr)
Other versions
EP0307774A2 (en
Inventor
Marc Ellous Parham
Santosh Raina
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
WR Grace and Co Conn
Original Assignee
WR Grace and Co Conn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by WR Grace and Co Conn filed Critical WR Grace and Co Conn
Publication of EP0307774A2 publication Critical patent/EP0307774A2/en
Publication of EP0307774A3 publication Critical patent/EP0307774A3/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Tissue plasminogen activator (t-PA) is isolated from a source material (i.e., conditioned cell culture media, tissue preparations or body fluids) by contacting with fibrin that has been pretreated by washing with t-PA elution buffer to remove elutable proteins, separating the fibrin-bound t-PA, then washing and eluting the t-PA. Salts and excess buffer are removed by dialysis to yield a highly purified t-PA product.
EP88114583A 1987-09-16 1988-09-07 Process for purifying plasminogen activator Withdrawn EP0307774A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9703587A 1987-09-16 1987-09-16
US97035 1998-06-12

Publications (2)

Publication Number Publication Date
EP0307774A2 EP0307774A2 (en) 1989-03-22
EP0307774A3 true EP0307774A3 (en) 1990-02-28

Family

ID=22260492

Family Applications (1)

Application Number Title Priority Date Filing Date
EP88114583A Withdrawn EP0307774A3 (en) 1987-09-16 1988-09-07 Process for purifying plasminogen activator

Country Status (2)

Country Link
EP (1) EP0307774A3 (en)
JP (1) JPH01108985A (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1981001417A1 (en) * 1979-11-13 1981-05-28 S Husain Isolation of plasminogen activators useful as therapeutic and diagnostic agents
FR2614037A1 (en) * 1987-04-20 1988-10-21 Arche Srl Lab Process for extracting plasminogen tissue activator from pregnant sow ovary

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1981001417A1 (en) * 1979-11-13 1981-05-28 S Husain Isolation of plasminogen activators useful as therapeutic and diagnostic agents
FR2614037A1 (en) * 1987-04-20 1988-10-21 Arche Srl Lab Process for extracting plasminogen tissue activator from pregnant sow ovary

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, vol. 98, no. 1, 3rd January 1983, page 199, no. 2151x, Columbus, Ohio, US; P. WALLEN et al.: "Purification and identification of two structural variants of porcine tissue plasminogen activator by affinity adsorption on fibrin" & BIOCHIM. BIOPHYS. ACTA 1982, 719(2), 318-28 *
PROC. NATL. ACAD. SCI. USA, vol. 78, no. 7, July 1981, pages 4265-4269, Washington, DC., US; S.S. HUSAIN et al.: "Rapid purification of a high-affinity plasminogen activator from human blood plasma by specific adsorption on fibrin/celite" *

Also Published As

Publication number Publication date
EP0307774A2 (en) 1989-03-22
JPH01108985A (en) 1989-04-26

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