EP0307774A3 - Process for purifying plasminogen activator - Google Patents
Process for purifying plasminogen activator Download PDFInfo
- Publication number
- EP0307774A3 EP0307774A3 EP88114583A EP88114583A EP0307774A3 EP 0307774 A3 EP0307774 A3 EP 0307774A3 EP 88114583 A EP88114583 A EP 88114583A EP 88114583 A EP88114583 A EP 88114583A EP 0307774 A3 EP0307774 A3 EP 0307774A3
- Authority
- EP
- European Patent Office
- Prior art keywords
- plasminogen activator
- fibrin
- washing
- purifying plasminogen
- purifying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
Tissue plasminogen activator (t-PA) is isolated from a
source material (i.e., conditioned cell culture media,
tissue preparations or body fluids) by contacting with
fibrin that has been pretreated by washing with t-PA
elution buffer to remove elutable proteins, separating the
fibrin-bound t-PA, then washing and eluting the t-PA.
Salts and excess buffer are removed by dialysis to yield a
highly purified t-PA product.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US9703587A | 1987-09-16 | 1987-09-16 | |
US97035 | 1987-09-16 |
Publications (2)
Publication Number | Publication Date |
---|---|
EP0307774A2 EP0307774A2 (en) | 1989-03-22 |
EP0307774A3 true EP0307774A3 (en) | 1990-02-28 |
Family
ID=22260492
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP88114583A Withdrawn EP0307774A3 (en) | 1987-09-16 | 1988-09-07 | Process for purifying plasminogen activator |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP0307774A3 (en) |
JP (1) | JPH01108985A (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1981001417A1 (en) * | 1979-11-13 | 1981-05-28 | S Husain | Isolation of plasminogen activators useful as therapeutic and diagnostic agents |
FR2614037A1 (en) * | 1987-04-20 | 1988-10-21 | Arche Srl Lab | Process for extracting plasminogen tissue activator from pregnant sow ovary |
-
1988
- 1988-09-07 EP EP88114583A patent/EP0307774A3/en not_active Withdrawn
- 1988-09-14 JP JP22888888A patent/JPH01108985A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1981001417A1 (en) * | 1979-11-13 | 1981-05-28 | S Husain | Isolation of plasminogen activators useful as therapeutic and diagnostic agents |
FR2614037A1 (en) * | 1987-04-20 | 1988-10-21 | Arche Srl Lab | Process for extracting plasminogen tissue activator from pregnant sow ovary |
Non-Patent Citations (2)
Title |
---|
CHEMICAL ABSTRACTS, vol. 98, no. 1, 3rd January 1983, page 199, no. 2151x, Columbus, Ohio, US; P. WALLEN et al.: "Purification and identification of two structural variants of porcine tissue plasminogen activator by affinity adsorption on fibrin" & BIOCHIM. BIOPHYS. ACTA 1982, 719(2), 318-28 * |
PROC. NATL. ACAD. SCI. USA, vol. 78, no. 7, July 1981, pages 4265-4269, Washington, DC., US; S.S. HUSAIN et al.: "Rapid purification of a high-affinity plasminogen activator from human blood plasma by specific adsorption on fibrin/celite" * |
Also Published As
Publication number | Publication date |
---|---|
JPH01108985A (en) | 1989-04-26 |
EP0307774A2 (en) | 1989-03-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US5147790A (en) | Serum-independent human cell lines, process for producing same, and processes for producing proteins therefrom | |
IE36670B1 (en) | Method for isolation of antithrombin from animal material | |
IL79957A0 (en) | Purification and activation of proteins from insoluble inclusion bodies | |
ZA91500B (en) | Process for the production of thrombin and high purity thrombin preparations thereby obtained | |
ES2000030A6 (en) | Method of purifying factor VIII:C. | |
CA1267497C (en) | Protein recovery | |
EP0233013A3 (en) | Modified enzyme | |
FI893519A (en) | Method for preparing Lys plasminogen | |
ES8601236A1 (en) | Peptide derivatives and their salts, pharmaceutical preparations containing them, process and intermediates for their production. | |
DE3475871D1 (en) | Process for the preparation of a composition containing a factor viii(ahf) | |
DE3479503D1 (en) | Process for the preparation of an antithrombin iii-heparin, e.g. an antithrombin iii-heparinoid concentrate | |
EP0307774A3 (en) | Process for purifying plasminogen activator | |
CA2178208A1 (en) | Purification of vitamin-k dependent proteins by membrane chromatography | |
US4550080A (en) | Process for the preparation of a plasminogen activator | |
ES8703522A1 (en) | Process for the purification of plasminogen activators. | |
ZA88990B (en) | A process for the inactivation of viruses having envelopes in protein preparations prepared in vitro with the aid of a cell | |
EP0163751B1 (en) | Process for the preparation of a plasminogen activator | |
GB1293795A (en) | Improvements relating to anticoagulants | |
EP0201800A3 (en) | A cholesterol-rich fraction, process therefor and use thereof in cell culture | |
US2871165A (en) | Process for preparing pankrin | |
JPS5618591A (en) | Production of substance capable of differentiation and multiplication of stem cells of human granulocyte | |
CA811697A (en) | Process in the recovery of plasminogen from animal serum or animal plasma, preferably serum or plasma from pig's blood or ox blood | |
HUT49151A (en) | Process for production of biotinilated proteins | |
CA803983A (en) | Process for the recovery of plasminogen from animal blood serum or animal blood plasma, preferably from pig's blood | |
SU1356289A1 (en) | Method for separating staphylococcus enterotoxin a |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
AK | Designated contracting states |
Kind code of ref document: A2 Designated state(s): AT BE CH DE ES FR GB GR IT LI LU NL SE |
|
PUAL | Search report despatched |
Free format text: ORIGINAL CODE: 0009013 |
|
AK | Designated contracting states |
Kind code of ref document: A3 Designated state(s): AT BE CH DE ES FR GB GR IT LI LU NL SE |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 19900829 |