EP0299183A2 - Verfahren zum Steigern des Milcheiweissgehaltes bei Wiederkäuern - Google Patents

Verfahren zum Steigern des Milcheiweissgehaltes bei Wiederkäuern Download PDF

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Publication number
EP0299183A2
EP0299183A2 EP88108549A EP88108549A EP0299183A2 EP 0299183 A2 EP0299183 A2 EP 0299183A2 EP 88108549 A EP88108549 A EP 88108549A EP 88108549 A EP88108549 A EP 88108549A EP 0299183 A2 EP0299183 A2 EP 0299183A2
Authority
EP
European Patent Office
Prior art keywords
bacteria
milk
animal
lactic acid
ruminants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP88108549A
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English (en)
French (fr)
Other versions
EP0299183B1 (de
EP0299183A3 (de
Inventor
Endre Kvanta
Mats Fischier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medipharm AB
Original Assignee
Medipharm AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medipharm AB filed Critical Medipharm AB
Publication of EP0299183A2 publication Critical patent/EP0299183A2/de
Publication of EP0299183A3 publication Critical patent/EP0299183A3/de
Application granted granted Critical
Publication of EP0299183B1 publication Critical patent/EP0299183B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus

Definitions

  • Such animals are herein also called polystomachic animals or ruminants.
  • living or viable lactic acid producing bacteria in many cases can replace antibiotic substances in admixture with feed compositions.
  • the purpose of using this principle is to stabilize the intestine channel of the animal by an antagonistic effect based on a fight between lactic acid producing bacteria and pathogenic bacteria.
  • SE-A-6913996-2 discloses the use of a preparation containing living bacteria in connection with domestic animals.
  • Such preparations are generally called probiotics.
  • the predominant part of probiotics are bacteria belonging to lactic acid producing lactobacills or streptococcs.
  • a small part belongs to the genus Bacillus, for example Bacillus subtilis or Leuconostoc. The majority of these are homofermentative lactic acid fermenters. Bacillus completely diverges from lactic acid bacteria both as to a species and function; members thereof are to a great extent used for enzym production, for example amylase and protease. Nevertheless Bacillus is used in the form of spores for the same purpose as lactobacills, i.e. to stabilize the intestine flora and thus increase the yield by an improved feed rate and an improved meat yield.
  • LBC Lactiferm
  • Adjulac lactic acid bacteria
  • Toyocerin spores of Bacillus
  • probiotics are not administered to milk producing ruminants. The reason is that such animals are considered to be very stable as to their intestinal function.
  • the intestinal micro flora of ruminants is further very complicated and those skilled in the art have the general opinion that it is not possible to affect the bacterial balance in the rumen by way of preparations containing bacteria added externally but are of the opinion that said balance is controlled by the composition of the feed as a primary factor. In certain cases efforts have been made to use probiotics even in rumi­nants, i.e. against mastitis.
  • US-A 4 591 499 discloses a method of controlling mastitis in mammals com­prising introducing into the mammary gland an amount of non-patho­genic lactic acid producing live bacteria.
  • This treatment is based on the utilization of a bacterial antagonism between non-pathogenic lactic acid producing bacteria and pathogenic bacteria, e.g. coli bacteria, inhibiting the growth of the pathogenic bacteria.
  • a possible way of supplying ruminants with lactic acid bacteria might be with the aid of a silage, i.e. green feed, which has been fermented with lactic acid bacteria. However, during storage of silage the lactic acid bacteria die. Thus, in silage there is no probiotic effect of naturally occuring or added so called ensilaging inoculants (such products are available on the market) consisting of living lactic acid bacteria.
  • the present invention is based on an effect of lactic acid producing bacteria belonging to the genera Lactobacillus, Pediococcus, Streptococcus and Leuconostoc, and of amylase and protease producing Bacillus, (preferably B subtilis and B toyoi), which effect resides in the fact that after administration of a culture of bacteria belonging to any of these genera or mixtures thereof, the composition of the milk is so affected that a significant daily increase of the protein contents of the milk is obtained (a certain modest increase of the fat contents of the milk is also noticed).
  • the contents of the bacteria or mixtures thereof is not critical as long as the desired effect is obtained. However, a range of appro­ximately 103-108 CFU (colony forming unit) per animal and day has proved appropriate. The lower limit is of course dictated by the milk protein increasing effect and the upper limit is dictated by the desired absence of pathologic effects. Once the technical effect according to the invention has been established those skilled in the art are able to determine the contents which is suitable in each case.
  • an effective quantity of one or more of the lactic acid producing bacteria is supplied to the milk producing animal.
  • effective quantity is meant a sufficient number of CFU to produce the desired increase of the protein contents of the milk obtained from the milk producing animal.
  • a composition comprising a vehicle or a carrier, which is known per se, and which is so selected that it will promote the optimal growth of the bacteria in the stomach-­intestine system of the animal.
  • carrier is also used, which is quite conventional, to facilitate the supply of the bacteria in question.
  • there are two main categories of carriers or vehicles i.e. those based on proteins and those based on carbohydrates.
  • protein based carriers there are mentioned milk powder and amino acids and as non-limiting examples of carbohydrate based carriers there are mentioned starch, cellulose and derivatives thereof, e.g. ethylhydroxyethylcellulose, carbohydroxymethylcellulose, etc., and sugars, such as glucose, fructose, dextrose, etc.
  • starch cellulose and derivatives thereof, e.g. ethylhydroxyethylcellulose, carbohydroxymethylcellulose, etc.
  • sugars such as glucose, fructose, dextrose, etc.
  • the choice of carrier is not at all critical and is within the area of those skilled in the art.
  • the bacteria or expressed more correctly, the culture of the bacteria is according to a preferred embodiment supplied to the milk producing animal in the form of a paste, a dry powder, in solution, preferably an aqueous solution, or in another suitable form, for example in the form of a feed composition.
  • the culture of bacteria is administered directly into the rumen for example by way of a syringe.
  • the culture is injected admixed with a suitable carrier, for example in the form of an oil emulsion based on corn oil.
  • composition of the preparation con­taining the culture of the lactic acid producing bacteria can be varied broadly and that in principle every combination of the culture and any carrier known per se can be used as long as the intended increase of the protein contents of the milk is produced.
  • One skilled in the art can easily find the most appropriate combination.
  • the administration method is of course also dependent on the type of animal and the age thereof.
  • lactic acid producing bacteria there are various known strains of non-pathogenic lactic acid producing bacteria including the entire genera Lactobacillus, Strepto­coccus, Pediococcus, Leuconostoc and including some species of the genus Bacillus.
  • lactic acid producing species within these categories the following may be mentioned which have proved to be effective in performing the method of the present invention. They must not be regarded as limiting the invention.
  • Lactobacillus acidophilus Lactobacillus bulgaricus, Lactobacillus casei, Lacto­bacillus cellobiosus, Lactobacillus confusus, Lactobacillus fermentum, Lactobacillus lactis, Lactobacillus leichmannii, Lacto­bacillus ruminis, Lactobacillus thermophilus, Streptococcus lactus, Streptococcus cremoris, Streptococcus thermophilus, Streptococcus faecium, Bacillus subtilis and Bacillus toyoi.
  • the culture of the bacteria in question or mixtures thereof in a most preferred embodiment is used in the form of a freeze-dried composition comprising conventional additives, such as cryoprotectant compounds, vitamins, trace elements and agents promoting free-flowing properties of the composition.
  • a characterizing feature of the bacteria providing a significant increase of milk protein is that they are isolated from other organisms than milk producing ruminants or are isolated from fresh green fodder or soil and that they are cultivated in vitro in such a way that a feasible amount of a mass of bacteria is obtained.
  • the mass of bacteria thus obtained is used either as such in appropriate dosis or in admixture with other masses of bacteria, or is dried in an optional way and is supplied to the animal (orally) either in the form of a paste, a powder, a solution or mixed into feed.
  • Cows were fed twice a day and each time they received 1 deciliter of a powdery preparation containing 108 living freeze-dried bacteria either in a pure form belonging to one of the genera Lactobacillus, Pediococcus Streptococcus, Leuconostoc or spores of Bacillus (subtilis or toyoi) or mixtures thereof, and further containing a carrier or a vehicle, the purpose of which being to support the growth of the bacteria in the rumen.
  • the effect of the bacteria thus added is not observed until after about 4 days, the milk protein contents gradually increasing within a couple of days to a significantly higher level. On the other side, the effect of the bacteria decreases about 4 days after the supply has been finished.
  • Dairy cows were fed 4-5 kg (DM) grass silage, 6 kg hay, 2 kg straw and a concentrate mix (grain and beet pulp) according to milk yield. Feeding was twice daily and all feeds were offered simultaneously.
  • Dairy cows were fed 2 kg (DM) grass silage, hay ad lib. and 8 kg commercial concentrate mix.
  • the cows were chosen based on an expected milk production of more than 25 kg milk per day.
  • the cows on all farms were of the Swedish Red and White Breed which has a milk composition of approximately 3.5 % protein and 4.0 % fat.
  • Milk samples were taken 3 days a week, morning and evening, and analyzed on "Milco-scan 133" (infra-red scanning) for protein, fat and lactose.
  • the most important dependent variable was assumed to be milk protein. Assuming an average of 3.5 % milk protein, an expected increase of 0.1 units, coefficient of variation of 0.6 and 90 % certainty for Type I and Type II errors, eight cows were needed on each farm to show differences both within and between farms. Fourteen cows were chosen as a safety margin. 90 % certainty was chosen as this was primarily a pilot study. The low number of cows needed can be attributed to the small variation seen in milk protein content. 0ther parameters, such as fat, have higher variations and the statistical model was not chosen to show significant differences in these, even if FCM, milk fat and milk yield were analyzed as dependent variables.
  • the protein production is increased by 40 g per day when the cows received bacteria preparations. corresponding to 2 x 108 living bacteria daily.
  • the above examples show a completely new and unexpected effect i.e. a significant increase of the milk protein of ruminants which is brought about by bacteria preparations supplied to the milk producing animal, preferably on a daily base.
  • a daily increase of the protein contents of the milk amounting to about 40 g per cow or about 0.25 % in the case of all ruminants, i.e. cows, sheep or goats, without a simultaneous increased feed consumption is of vital economical importance especially from a global point of view.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Physiology (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
EP88108549A 1987-06-11 1988-05-28 Verfahren zum Steigern des Milcheiweissgehaltes bei Wiederkäuern. Expired - Lifetime EP0299183B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE8702435A SE8702435L (sv) 1987-06-11 1987-06-11 Saett att oeka proteinhalten i mjoelk hos mjoelkproducerande djur
SE8702435 1987-06-11

Publications (3)

Publication Number Publication Date
EP0299183A2 true EP0299183A2 (de) 1989-01-18
EP0299183A3 EP0299183A3 (de) 1990-08-29
EP0299183B1 EP0299183B1 (de) 1994-06-01

Family

ID=20368824

Family Applications (1)

Application Number Title Priority Date Filing Date
EP88108549A Expired - Lifetime EP0299183B1 (de) 1987-06-11 1988-05-28 Verfahren zum Steigern des Milcheiweissgehaltes bei Wiederkäuern.

Country Status (6)

Country Link
US (1) US5093121A (de)
EP (1) EP0299183B1 (de)
AT (1) ATE106190T1 (de)
DK (1) DK317988A (de)
FI (1) FI882707A (de)
SE (1) SE8702435L (de)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU652815B2 (en) * 1991-04-08 1994-09-08 Quest International B.V. Probiotic
WO1997029645A1 (en) * 1996-02-14 1997-08-21 Biofeed (Thailand) Co., Ltd. Animal feed
KR100391267B1 (ko) * 2001-03-19 2003-07-12 학교법인 건국대학교 남은 음식물을 이용한 발효사료 및 이의 제조방법
US10961559B2 (en) 2016-04-26 2021-03-30 The State of Israel Ministry of Agriculture & Rural Development, Agricultural Research Organization (ARO) (Volcani Center) Regulation of feed efficiency and methane production in ruminating animals
US11951138B2 (en) 2013-03-14 2024-04-09 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Microbial compositions comprising rumen microflora and uses thereof

Families Citing this family (19)

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Publication number Priority date Publication date Assignee Title
GB9200891D0 (en) * 1992-01-16 1992-03-11 Mann Stephen P Formulation of microorganisms
US5534271A (en) * 1994-11-16 1996-07-09 Nutrition Physiology Process for improving the utilization of feedstuffs by ruminants
US5529793A (en) * 1994-11-16 1996-06-25 Nutrition Physiology Corporation Compositions for improving the utilization of feedstuffs by ruminants
US5656268A (en) * 1995-04-21 1997-08-12 Sorodsky; Michael Biological product
US7074603B2 (en) 1999-03-11 2006-07-11 Zeachem, Inc. Process for producing ethanol from corn dry milling
PL207932B1 (pl) 1999-03-11 2011-02-28 Zeachem Inc Sposób wytwarzania etanolu
US20040028665A1 (en) * 2002-01-08 2004-02-12 Garner Bryan E. Compositions and methods for inhibiting pathogenic growth
US7291326B2 (en) * 2003-01-06 2007-11-06 Nutrition Physiology Corporation Compositions and methods for reducing the pathogen content of meat and meat products
CA2500916A1 (en) * 2002-10-08 2004-04-08 Leanne Gail Robinson Substitute for animal protein in cattle feed
AU2004269388B2 (en) * 2003-08-29 2009-09-17 Nutrition Physiology Company, Llc. Methods for detecting and quantifying specific microorganisms
US20050048515A1 (en) * 2003-08-29 2005-03-03 Garner Bryan E. Methods for detecting and quantifying specific probiotic microorganisms in animal feed
MX306561B (es) * 2004-01-29 2013-01-09 Zeachem Inc Recuperacion de acidos organicos.
WO2008036719A2 (en) * 2006-09-19 2008-03-27 Powlen Owen J Animal feed supplements and uses thereof
CN101536732B (zh) * 2008-03-18 2012-07-04 安琪酵母股份有限公司 用作奶牛饲料添加剂的含酵母组合物
WO2015061789A2 (en) 2013-10-25 2015-04-30 Nch Corporation Delivery system and probiotic composition for animals and plants
US10766799B2 (en) 2014-05-23 2020-09-08 Nch Corporation Method for improving quality of aquaculture pond water using a nutrient germinant composition and spore incubation method
WO2015179788A1 (en) 2014-05-23 2015-11-26 Nch Corporation Method for improving quality of aquaculture pond water
WO2016060935A2 (en) 2014-10-08 2016-04-21 Novozymes A/S Compositions and methods of improving the digestibility of animal feed
US11401500B2 (en) 2018-08-29 2022-08-02 Nch Corporation System, method, and composition for incubating spores for use in aquaculture, agriculture, wastewater, and environmental remediation applications

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB357498A (en) * 1929-03-22 1931-09-24 Georgio Forti New biochemical product for the rationalization of cattle foods and the process of manufacture thereof
GB757630A (en) * 1954-01-22 1956-09-19 Tetsuo Katsube Animal fodder
US3875306A (en) * 1969-11-24 1975-04-01 Wenner Gren Medical Lab Aktieb Animal feed supplement
US3956482A (en) * 1973-06-25 1976-05-11 W. R. Grace & Co. Milk production

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07115643A (ja) * 1993-10-19 1995-05-02 Toyota Motor Corp 車載用撮像装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB357498A (en) * 1929-03-22 1931-09-24 Georgio Forti New biochemical product for the rationalization of cattle foods and the process of manufacture thereof
GB757630A (en) * 1954-01-22 1956-09-19 Tetsuo Katsube Animal fodder
US3875306A (en) * 1969-11-24 1975-04-01 Wenner Gren Medical Lab Aktieb Animal feed supplement
US3956482A (en) * 1973-06-25 1976-05-11 W. R. Grace & Co. Milk production

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JOURNAL OF DAIRY SCIENCE, vol. 69, suppl. 1, 1986, page 140, Champaign, US; R.J. GRANT et al.: "Performance of dairy cows fed alfalfa silage inoculated with lactobacillus microorganisms" *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU652815B2 (en) * 1991-04-08 1994-09-08 Quest International B.V. Probiotic
WO1997029645A1 (en) * 1996-02-14 1997-08-21 Biofeed (Thailand) Co., Ltd. Animal feed
KR100391267B1 (ko) * 2001-03-19 2003-07-12 학교법인 건국대학교 남은 음식물을 이용한 발효사료 및 이의 제조방법
US11951138B2 (en) 2013-03-14 2024-04-09 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Microbial compositions comprising rumen microflora and uses thereof
US10961559B2 (en) 2016-04-26 2021-03-30 The State of Israel Ministry of Agriculture & Rural Development, Agricultural Research Organization (ARO) (Volcani Center) Regulation of feed efficiency and methane production in ruminating animals
US11939620B2 (en) 2016-04-26 2024-03-26 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Regulation of feed efficiency and methane production in ruminating animals

Also Published As

Publication number Publication date
SE8702435L (sv) 1988-12-12
DK317988D0 (da) 1988-06-10
EP0299183B1 (de) 1994-06-01
FI882707A (fi) 1988-12-12
FI882707A0 (fi) 1988-06-08
ATE106190T1 (de) 1994-06-15
DK317988A (da) 1988-12-12
SE8702435D0 (sv) 1987-06-11
EP0299183A3 (de) 1990-08-29
US5093121A (en) 1992-03-03

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