EP0285646A4 - Intrazellular geregelte beförderung von expressionsprodukten. - Google Patents

Intrazellular geregelte beförderung von expressionsprodukten.

Info

Publication number
EP0285646A4
EP0285646A4 EP19870906870 EP87906870A EP0285646A4 EP 0285646 A4 EP0285646 A4 EP 0285646A4 EP 19870906870 EP19870906870 EP 19870906870 EP 87906870 A EP87906870 A EP 87906870A EP 0285646 A4 EP0285646 A4 EP 0285646A4
Authority
EP
European Patent Office
Prior art keywords
gene
region
peptide
sequence
transit peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19870906870
Other languages
English (en)
French (fr)
Other versions
EP0285646A1 (de
Inventor
Luca Comai
David M Stalker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Calgene LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene LLC filed Critical Calgene LLC
Publication of EP0285646A1 publication Critical patent/EP0285646A1/de
Publication of EP0285646A4 publication Critical patent/EP0285646A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/08—Fusion polypeptide containing a localisation/targetting motif containing a chloroplast localisation signal
EP19870906870 1986-09-26 1987-09-22 Intrazellular geregelte beförderung von expressionsprodukten. Withdrawn EP0285646A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US91240886A 1986-09-26 1986-09-26
US912408 1986-09-26

Publications (2)

Publication Number Publication Date
EP0285646A1 EP0285646A1 (de) 1988-10-12
EP0285646A4 true EP0285646A4 (de) 1989-12-18

Family

ID=25431868

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19870906870 Withdrawn EP0285646A4 (de) 1986-09-26 1987-09-22 Intrazellular geregelte beförderung von expressionsprodukten.

Country Status (4)

Country Link
EP (1) EP0285646A4 (de)
JP (1) JPH01501038A (de)
AU (1) AU620317B2 (de)
WO (1) WO1988002402A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5538878A (en) * 1988-08-08 1996-07-23 Calgene, Inc. Superoxide dismutase expression in plants
WO1990002172A1 (en) * 1988-08-18 1990-03-08 Calgene, Inc. Plant elongation factor, promoters, coding sequences and uses
AU644619B2 (en) * 1989-12-21 1993-12-16 Advanced Technologies (Cambridge) Limited Modification of plant metabolism
ATE212670T1 (de) * 1990-06-18 2002-02-15 Monsanto Technology Llc Erhöhter stärkegehalt in pflanzen
FR2673643B1 (fr) * 1991-03-05 1993-05-21 Rhone Poulenc Agrochimie Peptide de transit pour l'insertion d'un gene etranger dans un gene vegetal et plantes transformees en utilisant ce peptide.
USRE36449E (en) * 1991-03-05 1999-12-14 Rhone-Poulenc Agro Chimeric gene for the transformation of plants
WO1998010074A2 (de) * 1996-09-04 1998-03-12 Basf Aktiengesellschaft Adenylosuccinat synthetase
DE10014412A1 (de) * 2000-03-24 2001-10-04 Mpb Cologne Gmbh Molecular Pla Expressionsvektoren zur Anreicherung eines rekombinant hergestellten Proteins in unterschiedlichen Zellkompartimenten
US7105718B2 (en) 2000-03-31 2006-09-12 The Regents Of The University Of Colorado Compositions and methods for regulating metabolism in plants
AU2001251132A1 (en) * 2000-03-31 2001-10-15 University Technology Corporation Expression of uncoupling protein (ucp) in plants
FR2841247B1 (fr) 2002-06-21 2004-10-22 Genoplante Valor Peptide d'adressage plastidial
FR2961375B1 (fr) 2010-06-16 2016-05-13 Inst De Rech Pour Le Dev (Ird) Surproduction d'acide jasmonique dans des plantes transgeniques
FR2984076A1 (fr) 2011-12-15 2013-06-21 Inst Rech Developpement Ird Surproduction de jasmonates dans des plantes transgeniques

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0189707A2 (de) * 1984-12-28 1986-08-06 Plant Genetic Systems N.V. Rekombinante DNA, die in pflanzliche Zellen eingebracht werden kann

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0189707A2 (de) * 1984-12-28 1986-08-06 Plant Genetic Systems N.V. Rekombinante DNA, die in pflanzliche Zellen eingebracht werden kann

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
SCIENCE, vol. 233, 25th July 1986, pages 478-481; D.M. SHAH et al.: "Engineering herbicide tolerance in transgenic plants" *
See also references of WO8802402A1 *
THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 263, no. 29, 15th October 1988, pages 15104-15109, The American Society for Biochemistry and Molecular Biology, US; L. COMAI et al.: "Chloroplast transport of a ribulose bisphosphate carboxylase small subunit-5-enolpyruvyl 3-phosphoshikimate synthase chimeric protein requires part of the mature small subunit in addition to the transit peptide" *
TIBTECH, vol. 5, no. 2, February 1987, & 7TH INTERNATIONAL SYMPOSIUM ON THE STRUCTURE AND FUNCTION OF PLANT LIPIDS, University of California, Davis, California, US, 27th July - 1st August 1986, pages 40-47, Elsevier Science Publishers, B.V., Amsterdam, NL; V.C. KNAUF: "The application of genetic engineering to oilseed crops" *
WORLD BIOTECH: REPORT, vol. 2, 1985, pages 97-108; B.J. MAZUR et al.: "Cloning herbicide resistance genes into and out of plants" *

Also Published As

Publication number Publication date
AU8079787A (en) 1988-04-21
EP0285646A1 (de) 1988-10-12
AU620317B2 (en) 1992-02-20
WO1988002402A1 (en) 1988-04-07
JPH01501038A (ja) 1989-04-13

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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17P Request for examination filed

Effective date: 19880616

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH DE FR GB IT LI LU NL SE

RIN1 Information on inventor provided before grant (corrected)

Inventor name: STALKER, DAVID, M.

Inventor name: COMAI, LUCA

Inventor name: KNAUF, VIC, CHARLES

A4 Supplementary search report drawn up and despatched

Effective date: 19891218

17Q First examination report despatched

Effective date: 19910920

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 19920925