EP0180591B1 - Dispensing device for liquids - Google Patents

Dispensing device for liquids Download PDF

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Publication number
EP0180591B1
EP0180591B1 EP19850901444 EP85901444A EP0180591B1 EP 0180591 B1 EP0180591 B1 EP 0180591B1 EP 19850901444 EP19850901444 EP 19850901444 EP 85901444 A EP85901444 A EP 85901444A EP 0180591 B1 EP0180591 B1 EP 0180591B1
Authority
EP
European Patent Office
Prior art keywords
dispensing device
duct
filling
block
filling ducts
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
EP19850901444
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German (de)
French (fr)
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EP0180591A1 (en
Inventor
Eike BÖHME
Peter Rubenzer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Prolic AG
Original Assignee
Prolic AG
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Filing date
Publication date
Application filed by Prolic AG filed Critical Prolic AG
Publication of EP0180591A1 publication Critical patent/EP0180591A1/en
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Publication of EP0180591B1 publication Critical patent/EP0180591B1/en
Expired legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0203—Burettes, i.e. for withdrawing and redistributing liquids through different conduits
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/8593—Systems
    • Y10T137/86381—Head-establishing standpipe or expansion chamber [e.g., surge tanks]

Definitions

  • the invention relates to a dispensing device for liquids, in particular for loading cavities in titer plates, with a horizontal distributor pipe connected to a pump via a line, from which at least two, preferably eight, filling pipes lead radially away.
  • the Elisa method enzyme linked immunosorbent assay
  • the various methods of this system are used to measure antigens and / or antibodies.
  • antibodies are absorbed on the titer plate in a first process step, then the titer plate is washed, then in a further process step the addition of a residual solution containing the antigen is carried out, followed by washing again, followed by enzyme-labeled specific antibodies the titer plate is given, then the titer plate is washed again, after which the enzyme substrate is added.
  • the actual measurement is carried out in a photometer.
  • microtiter plates currently in use have 96 cavities, each of which corresponds practically to a cuvette.
  • a dispenser of the type mentioned in the opening paragraph is described in US-A-3357606.
  • a block is connected to a feed line and filling tubes lead away from this block.
  • a ball valve is provided in each fill tube, and attempts have been made to achieve dispensing levelness by precisely adjusting these valves.
  • the object of the invention is to significantly improve the dispensing levelness. No special adjustment work should be necessary.
  • a quantity of 0.4 ml is dispensed from the conventional piston stroke pumps per pump cycle, so that 50 11 liters of test liquid are dispensed per cavity of the microtiter plate in a dispensing device with eight filling tubes. It has proven to be advantageous for the delivery accuracy if the line between the distributor pipe and the pump is made of elastic material in order to be able to somewhat compensate for the relatively hard pump shocks. It should also be pointed out that in the dispensing device according to the invention, the liquid in the filling pipes is withdrawn by about 1-2 mm at the end of each pumping process, which reliably prevents dripping.
  • An embodiment of the invention provides that the diversion areas of the filling pipes are horizontally straight.
  • a particularly advantageous embodiment of the invention is characterized in that between the distributor pipe and the compensating pipe are two sections of the filling pipes leading upwards and leading away from the compensating pipe more than two diversion areas with the subsequent drainage areas of the filling pipes.
  • the drainage areas of the filling tubes are advantageously formed by metallic needles.
  • Another exemplary embodiment provides that the distributor pipe, the sections leading upwards and the discharge areas of the filling pipes and possibly the compensating pipe are incorporated in a common block in which the needles are held.
  • the block can be made of both plastic and stainless steel.
  • the block is advantageously worked in two parts, the parting plane between the block parts being a cut through the pipes.
  • the block has a lower holding strip for the needle.
  • FIG. 1 shows a section through a block according to the invention with a distributor pipe, compensating pipe and filling pipe
  • FIG. 2 shows a section along the line 111-111 of FIG. 1
  • FIG. 3 shows a section along the line IV-IV of FIG . 1.
  • the remaining parts of the dispensing device such as the pump, control device and liquid container, are not particularly shown in the figures of the drawing, they are manufactured according to the conventional state of the art.
  • a line 2 leads from the pump 1 to the connection 3 in the block 4 according to the invention and in this the liquid is first led into a horizontally oriented distributor pipe 5.
  • two filling tubes 6 lead upward into a compensating tube 7 from the distributor tube within the block 4.
  • the diversion areas 8 are incorporated in block 4.
  • the drainage areas 9 of the filling tubes 6 are formed in the exemplary embodiment by separate needles.
  • the block 4 is formed in two parts, the distributor pipe 5, the compensating pipe 7 and the two sections of the filling pipes 6 leading upwards being formed in a part 4 ', while in the second part 4 "the horizontal ones Redirection areas 8 of the filling pipes 6.
  • the needles, which form the drainage areas 9, are also stored in the second part 4 "of the block 4, which is provided at the top and bottom with a retaining strip 10 for the needles.
  • the needles in the exemplary embodiment shown have a diameter of 0.6 mm and a length of 21 mm.
  • the speed of the liquid in the needles is approximately 0.7 m / sec.

Abstract

A dispensing device enables a liquid to be delivered to the cavities of a titration plate. In order to obtain optimum quality of delivery, charging pipes lead from a distributing pipe first upwards, and then, after passing a bypass area, downwards to a discharge area.

Description

Die Erfindung bezieht sich auf eine Dispensiereinrichtung für Flüssigkeiten, insbesondere für das Beschicken von Kavitäten von Titerplatten, mit einem über eine Leitung an eine Pumpe angeschlossenen horizontalen Verteilerrohr, von dem mindestens zwei, vorzugsweise acht, Füllrohre radial wegführen.The invention relates to a dispensing device for liquids, in particular for loading cavities in titer plates, with a horizontal distributor pipe connected to a pump via a line, from which at least two, preferably eight, filling pipes lead radially away.

In der medizinischen Labortechnik wird beispielsweise zur Messung von Antigenen und/oder Antikörpern das Elisa-Verfahren angewendet (Enzym linked immunosorbent assay), bzw. die verschiedenen Verfahren dieses Systems. Es werden beispielsweise zur Messung von Antigenen in einem ersten Verfahrensschritt Antikörper an der Titerplatte absorbiert, anschließend wird die Titerplatte gewaschen, dann erfolgt in einem weiteren Verfahrensschritt die Beimengung einer Restlösung, welche das Antigen enthält, darauf erfolgt wiederum eine Waschung, worauf enzymmarkierte spezifische Antikörper auf die Titerplatte gegeben werden, dann erfolgt wieder ein Waschen der Titerplatte, worauf die Beimengung des Enzymsubstrates erfolgt.In medical laboratory technology, for example, the Elisa method (enzyme linked immunosorbent assay) or the various methods of this system are used to measure antigens and / or antibodies. For example, to measure antigens, antibodies are absorbed on the titer plate in a first process step, then the titer plate is washed, then in a further process step the addition of a residual solution containing the antigen is carried out, followed by washing again, followed by enzyme-labeled specific antibodies the titer plate is given, then the titer plate is washed again, after which the enzyme substrate is added.

Die eigentliche Messung erfolgt in einem Photometer.The actual measurement is carried out in a photometer.

Die derzeit in Gebrauch befindlichen Mikrotiterplatten weisen 96 Kavitäten auf, von denen jede praktisch einer Küvette entspricht.The microtiter plates currently in use have 96 cavities, each of which corresponds practically to a cuvette.

Mit den bekannten Dispensiereinrichtungen werden jeweils acht Kavitäten auf einmal gefüllt.With the known dispensing devices, eight cavities are filled at a time.

Eines der wesentlichen Probleme, die dabei auftreten, ist, daß die Füllmenge für jede Kavität möglichst gleich bleiben soll und daß weiters sowohl die Bildung von Luftblasen als auch ein Nachtropfen absolut sicher verhindert werden muß.One of the main problems that arise is that the filling quantity for each cavity should remain as constant as possible and that both the formation of air bubbles and dripping must be prevented with absolute certainty.

Das Problem der gleichmäßigen Abgabe wird noch dadurch erschwert, daß mit ein und derselben Vorrichtung bei verschiedenen Testvorgängen Flüssigkeiten unterschiedlicher Viskosität abgegeben werden müssen.The problem of uniform dispensing is made even more difficult by the fact that liquids and different viscosities have to be dispensed with one and the same device in different test processes.

Eine Dispensiereinrichtung der eingangs erwähnten Art ist in der US-A-3357606 beschrieben. Dabei ist ein Block an eine Zuführleitung angeschlossen und von diesem Block führen Füllröhrchen weg. In jedem Füllrohr ist ein Kugelventil vorgesehen, und es wurde versucht, die Abgabeegalität durch genaue Einstellung dieser Ventile zu erreichen.A dispenser of the type mentioned in the opening paragraph is described in US-A-3357606. A block is connected to a feed line and filling tubes lead away from this block. A ball valve is provided in each fill tube, and attempts have been made to achieve dispensing levelness by precisely adjusting these valves.

Mit den herkömmlichen Kolbenhub- oder Membranpumpen wird eine auf 5 % genaue Abgabeegalität erreicht. Aufgabe der Erfindung ist es, die Abgabeegalität wesentlich zu verbessern. Dabei sollen keine besonderen Einstellarbeiten notwendig sein.With conventional piston stroke or diaphragm pumps, delivery levelness is accurate to 5%. The object of the invention is to significantly improve the dispensing levelness. No special adjustment work should be necessary.

Dies wird erfindungsgemäß dadurch erreicht, daß am oberen Ende des nach oben führenden Abschnittes der Füllrohre ein parallel zum Verteilerrohr ausgerichtetes Ausgleichsrohr ist, an das die Umleitungsbereiche der Füllrohre anschließen.This is achieved according to the invention in that at the upper end of the section of the filling pipes leading upwards there is a compensating pipe aligned parallel to the distributor pipe, to which the diversion areas of the filling pipes connect.

Mit der erfindungsgemäßen Einrichtung werden Volumensunterschiede bei den Füllungen der Kavitäten von nur ± 1 % erzielt.With the device according to the invention, volume differences in the fillings of the cavities of only ± 1% are achieved.

In diesem Zusammenhang ist zu beachten, daß nicht die Gesamtmenge der in eine Titerplatte abgefüllten Flüssigkeitsmenge von Bedeutung ist und diesbezügliche Volumensschwankungen, sondern das Volumensverhältnis der Flüssigkeiten in den einzelnen Kavitäten zueinander.In this context, it should be noted that it is not the total amount of liquid filled into a titer plate and the relevant volume fluctuations that are important, but the volume ratio of the liquids in the individual cavities to one another.

Von den herkömmlichen Kolbenhubpumpen wird pro Pumpenzyklus eine Menge von 0,4 ml abgegeben, so daß bei einer Dispensiereinrichtung mit acht Füllrohren 50 111 Testflüssigkeit pro Kavität der Mikrotiterplatte abgegeben werden. Es hat sich dabei als vorteilhaft für die Abgabegenauigkeit erwiesen, wenn die Leitung zwischen dem Verteilerrohr und der Pumpe aus elastischen Material ist, um die relativ harten Pumpenstöße etwas ausgleichen zu können. Es ist noch darauf hinzuweisen, daß bei der erfindungsgemäßen Dispensiereinrichtung am Ende jedes Pumpvorganges die Flüssigkeit in den Füllrohren um etwa 1 - 2 mm zurückgezogen wird, wodurch ein Nachtropfen sicher verhindert wird.A quantity of 0.4 ml is dispensed from the conventional piston stroke pumps per pump cycle, so that 50 11 liters of test liquid are dispensed per cavity of the microtiter plate in a dispensing device with eight filling tubes. It has proven to be advantageous for the delivery accuracy if the line between the distributor pipe and the pump is made of elastic material in order to be able to somewhat compensate for the relatively hard pump shocks. It should also be pointed out that in the dispensing device according to the invention, the liquid in the filling pipes is withdrawn by about 1-2 mm at the end of each pumping process, which reliably prevents dripping.

Ein Ausführungsbeispiel der Erfindung sieht vor, daß die Umleitungsbereiche der Füllrohre horizontal geradlinig sind.An embodiment of the invention provides that the diversion areas of the filling pipes are horizontally straight.

Ein besonders vorteilhaftes Ausführungsbeispiel der Erfindung ist dadurch gekennzeichnet, daß zwischen dem Verteilerrohr und dem Ausgleichsrohr zwei nach oben führende Abschnitte der Füllrohre sind und vom Ausgleichsrohr mehr als zwei Umleitungsbereiche mit den anschließenden Abflußbereichen der Füllrohre wegführen.A particularly advantageous embodiment of the invention is characterized in that between the distributor pipe and the compensating pipe are two sections of the filling pipes leading upwards and leading away from the compensating pipe more than two diversion areas with the subsequent drainage areas of the filling pipes.

Die Abflußbereiche der Füllrohre werden vorteilhaft von metallischen Nadeln gebildet.The drainage areas of the filling tubes are advantageously formed by metallic needles.

Ein weiteres Ausführungsbeispiel sieht vor, daß das Verteilerrohr, die nach oben führenden Abschnitte und die Ableitungsbereiche der Füllrohre und gegebenenfalls das Ausgleichsrohr in einem gemeinsamen Block eingearbeitet sind, in dem die Nadeln gehalten sind. Der Block kann sowohl aus Kunststoff als auch aus Edelstahl gefertigt sein.Another exemplary embodiment provides that the distributor pipe, the sections leading upwards and the discharge areas of the filling pipes and possibly the compensating pipe are incorporated in a common block in which the needles are held. The block can be made of both plastic and stainless steel.

Vorteilhaft ist der Block zweiteilig gearbeitet, wobei die Trennungsebene zwischen den Blockteilen gleich ein Schnitt durch die Rohre ist.The block is advantageously worked in two parts, the parting plane between the block parts being a cut through the pipes.

Vorteilhaft ist vorgesehen, daß der Block eine untere Halteleiste für die Nadel aufweist.It is advantageously provided that the block has a lower holding strip for the needle.

Nachfolgend wird ein Ausführungsbeispiel der Erfindung anhand der Figuren der beiliegenden Zeichnung beschrieben. Fig. 1 zeigt einen Schnitt durch einen erfindungsgemäßen Block mit Verteilerrohr, Ausgleichsrohr und Füllrohr, die Fig. 2 zeigt einen Schnitt nach der Linie 111 - 111 der Fig. 1, die Fig. 3 zeigt einen Schnitt nach der Linie IV - IV der Fig. 1.An exemplary embodiment of the invention is described below with reference to the figures in the accompanying drawing. 1 shows a section through a block according to the invention with a distributor pipe, compensating pipe and filling pipe, FIG. 2 shows a section along the line 111-111 of FIG. 1, FIG. 3 shows a section along the line IV-IV of FIG . 1.

In den Figuren der Zeichnung sind die restlichen Teile der Abgabevorrichtung, wie Pumpe, Steuereinrichtung und Flüssigkeitsbehälter nicht besonders gezeigt, sie sind nach dem herkömmlichen Stand der Technik gefertigt.The remaining parts of the dispensing device, such as the pump, control device and liquid container, are not particularly shown in the figures of the drawing, they are manufactured according to the conventional state of the art.

Von der Pumpe 1 führt eine Leitung 2 zum Anschluß 3 im erfindungsgemäßen Block 4 und in diesem wird die Flüssigkeit zuerst in ein horizontal ausgerichtetes Verteilerrohr 5 geführt.A line 2 leads from the pump 1 to the connection 3 in the block 4 according to the invention and in this the liquid is first led into a horizontally oriented distributor pipe 5.

Im gezeigten Ausführungsbeispiel führen innerhalb des Blockes 4 vom Verteilerrohr zwei Füllrohre 6 nach oben in ein Ausgleichsrohr 7.In the exemplary embodiment shown, two filling tubes 6 lead upward into a compensating tube 7 from the distributor tube within the block 4.

Vom Ausgleichsrohr 7 führen acht Füllrohre 6 weg, und zwar zuerst mit horizontalen Umleitungsbereichen 8, schließlich mit vertikal nach unten führenden Abflußbereichen 9.Eight fill pipes 6 lead away from the compensating pipe 7, first with horizontal diversion areas 8 and finally with drainage areas 9 leading vertically downwards.

Die Umleitungsbereiche 8 sind, wie aus der Fig. 1 ersichtlich, im Block 4 eingearbeitet. Die Abflußbereiche 9 der Füllrohre 6 werden im Ausführungsbeispiel von separaten Nadeln gebildet.As can be seen in FIG. 1, the diversion areas 8 are incorporated in block 4. The drainage areas 9 of the filling tubes 6 are formed in the exemplary embodiment by separate needles.

Wie aus der Fig. 1 ersichtlich, ist der Block 4 zweiteilig ausgebildet, wobei in einem Teil 4' das Verteilerrohr 5, das Ausgleichsrohr 7 und die beiden nach oben führenden Abschnitte der Füllrohre 6 ausgebildet sind, während im zweiten Teil 4" Sich die horizontalen Umleitungsbereiche 8 der Füllrohre 6 befinden. Die Nadeln, die die Abflußbereiche 9 bilden, sind ebenfalls im zweiten Teil 4" des Blockes 4 gelagert, der oben und unten mit einer Halteleiste 10 für die Nadeln versehen ist.As can be seen from FIG. 1, the block 4 is formed in two parts, the distributor pipe 5, the compensating pipe 7 and the two sections of the filling pipes 6 leading upwards being formed in a part 4 ', while in the second part 4 "the horizontal ones Redirection areas 8 of the filling pipes 6. The needles, which form the drainage areas 9, are also stored in the second part 4 "of the block 4, which is provided at the top and bottom with a retaining strip 10 for the needles.

Ergänzend sei noch hinzugefügt, daß die Nadeln im gezeigten Ausführungsbeispiel einen Durchmesser von 0,6 mm und eine Länge von 21 mm haben. Die Geschwindigkeit der Flüssigkeit in den Nadeln beträgt ungefähr 0,7 m/sec.In addition, it should be added that the needles in the exemplary embodiment shown have a diameter of 0.6 mm and a length of 21 mm. The speed of the liquid in the needles is approximately 0.7 m / sec.

Claims (5)

1. A dispensing device for liquids, in particular for charging the cavities of titration plates, comprising a horizontal distributor duct (5) which is connected to a pump (1) by way of a conduit (2) and from which at least two and preferably eight filling ducts (6) lead away radially, which filling ducts firstly lead upwardly from the distributor duct (5) and then, downstream of a direction-changing region (8), lead downwardly into a discharge flow region (9), characterised in that provided at the upper end of the upwardly leading portions of the filling ducts (6) is a compensating duct (7) which is oriented parallel to the distributor duct (5) and to which the direction-changing regions (8) of the filling ducts (6) connect.
2. A dispensing device according to claim 1 characterised in that provided between the distributor duct (5) and the compensating duct (7) are two upwardly leading portions of the filling ducts (6) and more than two direction-changing regions (8), with the adjoining discharge flow regions (9) of the filling ducts (6), lead away from the compensating duct (7).
3. A dispensing device according to claim 2 characterised in that the distributor duct (5), the upwardly leading portions and the direction-changing regions (8) of the filling ducts (6) and possibly the compensating duct (7) are provided in a common block (4).
4. A dispensing device according to claim 3 characterised in that the block (4) is in two parts.
5. A dispensing device according to claim 3 characterised in that the block (4) has a lower bar portion (10) for needles forming the discharge flow regions (9).
EP19850901444 1984-04-10 1985-04-04 Dispensing device for liquids Expired EP0180591B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT1196/84 1984-04-10
AT119684A AT379455B (en) 1984-04-10 1984-04-10 DISPENSING DEVICE FOR LIQUIDS

Publications (2)

Publication Number Publication Date
EP0180591A1 EP0180591A1 (en) 1986-05-14
EP0180591B1 true EP0180591B1 (en) 1989-01-25

Family

ID=3508680

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19850901444 Expired EP0180591B1 (en) 1984-04-10 1985-04-04 Dispensing device for liquids

Country Status (6)

Country Link
US (1) US4779769A (en)
EP (1) EP0180591B1 (en)
JP (1) JPS61501868A (en)
AT (2) AT379455B (en)
DE (1) DE3567821D1 (en)
WO (1) WO1985004599A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19911456A1 (en) * 1999-03-08 2000-09-14 Jenoptik Jena Gmbh Multi-channel drop generator

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3357606A (en) * 1965-06-18 1967-12-12 James F Kahlenberg Fluid flow adjustment device for manifolded dispensing nozzles
FR1556997A (en) * 1966-09-01 1969-01-06
US3724984A (en) * 1970-09-22 1973-04-03 Eskimo Pie Corp Extruder nozzle distribution plate with a plurality of valve controlled grooves
SE355076B (en) * 1971-03-26 1973-04-02 Aga Ab
US3818931A (en) * 1972-06-08 1974-06-25 J Carpenter Water hammer arrester
US4488665A (en) * 1982-05-24 1984-12-18 Spraymation, Inc. Multiple-outlet adhesive applicator apparatus and method

Also Published As

Publication number Publication date
JPS61501868A (en) 1986-08-28
DE3567821D1 (en) 1989-03-02
EP0180591A1 (en) 1986-05-14
AT379455B (en) 1986-01-10
ATA119684A (en) 1985-05-15
US4779769A (en) 1988-10-25
WO1985004599A1 (en) 1985-10-24
ATE40309T1 (en) 1989-02-15

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