EP0148333B1 - Disposable pipette tip - Google Patents

Disposable pipette tip Download PDF

Info

Publication number
EP0148333B1
EP0148333B1 EP84112251A EP84112251A EP0148333B1 EP 0148333 B1 EP0148333 B1 EP 0148333B1 EP 84112251 A EP84112251 A EP 84112251A EP 84112251 A EP84112251 A EP 84112251A EP 0148333 B1 EP0148333 B1 EP 0148333B1
Authority
EP
European Patent Office
Prior art keywords
tip
pipette
collar
disposable
pipette tip
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
EP84112251A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP0148333A1 (en
Inventor
Fred Gardner Williams
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corp filed Critical Cetus Corp
Priority to AT84112251T priority Critical patent/ATE45304T1/de
Publication of EP0148333A1 publication Critical patent/EP0148333A1/en
Application granted granted Critical
Publication of EP0148333B1 publication Critical patent/EP0148333B1/en
Expired legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means

Definitions

  • the present invention relates to disposable tips for a pipette to take up and discharge accurately minute liquid quantities at any given level.
  • a pipette tip that may be automatically picked up by and ejected from a reciprocable pipette.
  • Such tip is preferably formed of a nonwetting plastic with a conical take up tip portion terminating at its base end in a generally cylindrical portion to mate with the pipette barrel.
  • the cylindrical portion has (1) a stop, preferably a shoulder at its junction with the conical tip, that defines a seat for the end of the pipette barrel and establishes a known and repeatable distance from the end of the pipette barrel to the intake and discharge level of the tip and (2) an integral, internal circumferential seal between the stop and the open end of the cylinder to impose a low friction, air-tight seal around the pipette barrel.
  • Pipettes to handle precise small amounts of liquid are known in the chemical, biological and biochemical fields. They are particularly useful for dispensing and transferring microtiter quantities of culture media, diluents, and other liquids from one location to another.
  • pipettes include a syringe with a reciprocable pump plunger operating in a barrel to aspirate liquid from or expel liquid into test tubes or wells in a microliter tray through a replaceable tip.
  • Such pipettes have generally been used either single or as a "ganged" group for hand manipulation.
  • An automatic liquid transfer system that employs pipettes has recently been developed by applicant.
  • the system is particularly directed to pick up and dispose of a replaceable tip after each liquid transfer to avoid cross-contamination or liquid carryover from one liquid volume, or sample, to another. Because the entire system is adapted to sequence automatically through a number of steps, each of which requires (1) an air-tight connection between the tip and the pipette nose or barrel, with a predeterminable friction for easy installation and removal and (2) a given, fixed distance from the inlet end of the tip to the end of the pipette barrel.
  • US-A-3 760 639 relates to a manually operable pipette assembly.
  • the assembly includes a housing having a pipette support, which terminates at its free end in a reduced diameter portion.
  • the reduced diameter portion is adapted to removable receive an expendable hollow pipette tip.
  • the pipette tip may be a disposable tip of high density, rigid polymeric plastic materials such as polypropylene.
  • the pipette tip has a conical exterior and a cylindrical hollow interior.
  • the upper end of the tip forms a cylindrical collar.
  • the diameter and the length of this collar correspond to the dimensions of the reduced diameter portion.
  • FR-A-2 021 566 relates to a manually operable pipette assembly comprising a pipette which may receive pipette tips at its forward end portion.
  • This end portion has a general cylindrical shape and comprises a circumferential groove in which an O-ring is received.
  • grease is applied to the O-ring. Then the pipette tip is placed on this end portion by twisting and pushing.
  • the pipette has a conical shaped interior followed by a cylindrical portion and a funnel shaped receiving portion. At the transmission from the cylindrical portion to the funnel shaped receiving portion a step is formed. When the pipette tip is placed on the end portion the O-ring reaches the step.
  • the invention is a disposable pipette tip comprising an elongated conical tube, the small end of which defines a passageway for liquid and the large end of which receives the end of a pipette
  • the tip includes a generally cylindrical collar for receiving the end of the pipette, the collar being axially aligned with and opening into the large end of the conical tube and having a stop for engaging the pipette to fix an accurately controlled distance from the end of the pipette to the small end of the pipette tip characterized in that the collar comprises an internal circumferential seal rib for forming a friction fit and seal with the sidewall of the pipette.
  • the stop of the collar provides a seat for the end of the pipette to provide a fixed and known length for the overall assembly.
  • capillary distances on the order of several hundredths of a centimeter, between the inlet end of the tip and the liquid surface can be machine controlled when the tip is withdrawn.
  • the inlet end of the tip may be accurately positioned at any other desired or given level in a microtiter plate well, such as at an oil-water interface.
  • the circumferential seal between the lumen of the collar and the pipette barrel assures minimum friction with assured air sealing for tips within reasonable manufacturing tolerances.
  • the circumferential rib seal also assures easy removal of the tip for disposal after a single use, particularly where a number of pipettes are ganged together and a single stripper simultaneously ejects the tips from their respective pipettes.
  • disposable pipette tip 10 includes an elongated, hollow conical tube section 11 terminating at its intake and discharge end 12 at a right angle to the axis of conical section 11.
  • Base end 13 of conical tube 11 merges into a cylindrical mounting collar 14.
  • collar 14 provides a mounting and sealing arrangement with nose 15 of pipette barrel 16 carried by mounting block 18.
  • a pump plunger 22 is arranged to reciprocate in bore 24 of pipette barrel 16 to aspirate or discharge liquid through end 12 of tip 10.
  • lower end 19 of nose 15 seats on ledge 17 formed by the transition between conical section 11 and cylindrical bore 20 of collar 14. Such seating of end 19 on ledge 17 fixes a pecise distance from block 18 to tip intake-discharge port 12.
  • radial projections other than a ledge or collar may be used to provide an axial stop for the end of the pipette barrel and that the projection need not be positioned at the transition between the conical and cylindrical section.
  • the stop may be defined by a rim at the mouth of the cylindrical section that is adapted to engage a radial collar or other radial member on the exterior of the pipette.
  • bore 20 of collar 14 includes an integral circumferential rib or ring 30.
  • rib 30 preferably has an axially deformed V-shape in cross section.
  • Such construction permits a low friction fit between the sidewalls of nose 15 and bore 20 of tip 10 both for insertion and removal while accommodating large manufacturing tolerance (several thousandths of a centimeter) in diameter and roundness of bore 20.
  • Such tolerances are particularly vital in molding of tips 10 preferably formed of thermoplastic or thermosetting materials, such as polypropylene, polyvinylchloride, and fluorocarbon polymers.
  • the internal sidewall of the collar may have a slow taper that does not interfere with the action of the stop but causes the pipette to form a seal with the collar sidewall.
  • upper end 32 preferably has a bell-shaped mouth that terminates in a reinforced upper rim portion 34.
  • the bell mouth permits easy access for the end 19 of nose 15 so that if the tip is not in the center of hole 31, as in Figure 1, the cylindrical portion of nose 15 and bore 20 will readily align with each other.
  • Further rim portion 34 provides a pressing surface radially displaced from pipette nose 15 which is readily engageable by a stripper member on an automatic stripper arm 36.
  • Replaceable tips in accordance with the present invention also include a plurality of three or more, preferably four, mounting splines 37, formed integral with the upper potion of conical section 11.
  • Splines 37 also provide thrust absorbing means so that the force of barrel 16 entering the collar and seating on ledge 17 does not place deforming stresses on intake-discharge end 12 of the tip.
  • splines 37 desirably extend axially along the exterior of the section 11 from transition ring 39, just below collar or ledge 17, and each terminates in the upper quarter or less of the conical section to form support legs to hold the tip, as shown.
  • spline ends 38 are at substantially right angles to the axis of the tip to hold the tip in such a vertical position when seated over hole 31 formed in a plate, tube or box support, such as 40, having a flat top.
  • hole 31 in box 40 is slightly larger than the greatest diameter of conical section 11 at the level of spline ends 38, but small enough to prevent wedging of splines 37 in hole 31.
  • splines 37 also provide minimum friction areas for absorbing thrust by ring 39 or cylindrical portion 14.
  • the length of splines 37 is such that their bottoms 38 are just above the axial center of gravity of tip 10 so that the tip does not wobble excessively in response to horizontal accelerations.
  • Such horizontal accelerations are important in high speed automatic liquid transfers and replacement of the tips on pipette nose 15. This is because excess wobble of the tip when it is being loaded onto nose 15 would require a pause sufficient to assure alignment.
  • the cylindrical portions such as bore 20 and splines 37 will be formed with a draft angle of less than 2° relative to the axis.
  • nose portion 15 generally includes a small draft angle along its cylindrical portion to assure easy entrance and release with respect to bore 20 as well as for sealing action around rib 30. Because rib 30 is capable of radially deforming to conform to irregularities in the diameter or roundness of the side wall of nose 15, an air-tight seal is obtained without reliance solely upon deformation of the sidewall of cylindrical portion 14 or pressure between nose end 19 and collar 17.
  • FIG. 6 illustrates alternate embodiments in the sealing and seating arrangements of the present invention.
  • seating ledge 17 may be formed as a plurality of ledges 47 which are molded into tip 10 to form a fixed end seat for end 19. Desirably three or more, preferably four, ledges 47 are used in such an " embodiment.
  • sealing between bore 20 and the cylindrical sidewall of nose 15 may be provided by an 0-ring 48 seated in groove 49 formed in nose 15.
  • Figure 6 also illustrates that nose 15 may be formed with a slightly tapered end portion 50 which may be spherical in configuration. Alternatively the taper of portion 50 may be conical and the end slightly chamferred to permit easy alignment and passage of nose end 19 into tip 10, including an integrally formed sealing rib.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Cold Cathode And The Manufacture (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • External Artificial Organs (AREA)
  • Materials For Medical Uses (AREA)
EP84112251A 1983-10-13 1984-10-11 Disposable pipette tip Expired EP0148333B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT84112251T ATE45304T1 (de) 1983-10-13 1984-10-11 Einmalig verwendbare pipettenspitze.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US54167883A 1983-10-13 1983-10-13
US541678 1990-06-21

Publications (2)

Publication Number Publication Date
EP0148333A1 EP0148333A1 (en) 1985-07-17
EP0148333B1 true EP0148333B1 (en) 1989-08-09

Family

ID=24160607

Family Applications (1)

Application Number Title Priority Date Filing Date
EP84112251A Expired EP0148333B1 (en) 1983-10-13 1984-10-11 Disposable pipette tip

Country Status (9)

Country Link
EP (1) EP0148333B1 (enrdf_load_stackoverflow)
JP (1) JPS60137443A (enrdf_load_stackoverflow)
AT (1) ATE45304T1 (enrdf_load_stackoverflow)
AU (1) AU565474B2 (enrdf_load_stackoverflow)
DE (2) DE148333T1 (enrdf_load_stackoverflow)
DK (1) DK490984A (enrdf_load_stackoverflow)
FI (1) FI843976L (enrdf_load_stackoverflow)
NO (1) NO844093L (enrdf_load_stackoverflow)
NZ (1) NZ209865A (enrdf_load_stackoverflow)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4863695A (en) * 1987-04-28 1989-09-05 Hewlett-Packard Company Pipette assembly
GB8808583D0 (en) * 1988-04-12 1988-05-11 Flow Lab Pipette tip pickup apparatus
DE3824767A1 (de) * 1988-07-21 1990-02-01 Eppendorf Geraetebau Netheler Aufsteckbare pipettenspitze in form eines entsprechend einem aufsteckkopfstueck, insbesondere konus einer pipette, wenigstens abschnittsweise ausgefuehrten gefaesses
US5200151A (en) * 1990-05-21 1993-04-06 P B Diagnostic Systems, Inc. Fluid dispensing system having a pipette assembly with preset tip locator
US5232669A (en) * 1991-11-08 1993-08-03 Abbott Laboratories Pipette tip with self-aligning and self-sealing features
US5496523A (en) * 1994-05-06 1996-03-05 Sorenson Bioscience Filtered micropipette tip for high/low volume pipettors
EP1876451A3 (en) 1998-07-27 2012-02-29 Hitachi, Ltd. Handling method of body fluid sample and analysis apparatus using the same
US6168761B1 (en) * 1998-11-06 2001-01-02 Rainin Instrument Co., Inc. Pipette with improved pipette tip and mounting shaft
WO2000027529A1 (en) * 1998-11-06 2000-05-18 Rainin Instrument Co., Inc. Pipette with improved pipette tip and mounting shaft
DE19917375C2 (de) 1999-04-16 2001-09-27 Hamilton Bonaduz Ag Bonaduz Pipettiereinheit
US6596240B2 (en) 2001-01-12 2003-07-22 Porex Corporation Pipette tip for easy mounting and ejecting from a pipette
US6973845B2 (en) * 2001-01-18 2005-12-13 Beckman Coulter, Inc. Low insertion force tip/mandrel
EP1329262B1 (de) 2002-01-16 2009-08-19 Brand Gmbh + Co Kg Pipette und Verfahren zur Herstellung einer Pipette
DE10222511B4 (de) * 2002-05-22 2005-03-17 Eppendorf Ag Pipettenspitze
EP1862219B1 (de) * 2006-05-29 2018-02-07 Qiagen GmbH Vorrichtung zur halterung von pipettenspitzen sowie pipettiervorrichtung
US7662344B2 (en) 2006-10-24 2010-02-16 Viaflo Corporation Locking pipette tip and mounting shaft
US7662343B2 (en) 2006-10-24 2010-02-16 Viaflo Corporation Locking pipette tip and mounting shaft
US8277757B2 (en) 2009-09-29 2012-10-02 Integra Biosciences Corp. Pipette tip mounting shaft
JP6780338B2 (ja) * 2016-07-22 2020-11-04 ぺんてる株式会社 ピペットチップとそのピペットチップに装着されるノズルとの連結構造
CN114904074A (zh) * 2022-05-17 2022-08-16 温州医科大学附属第二医院（温州医科大学附属育英儿童医院） 小鼠腹腔细胞提取装置

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3646817A (en) * 1968-10-25 1972-03-07 Bio Dynamics Inc Pipette
FI46328C (fi) * 1970-05-15 1973-03-12 Kone Oy Pipettikärkisarja erityisesti koneelliseen nesteannosteluun.
US3760639A (en) * 1972-10-20 1973-09-25 Bio Data Corp Pipette assembly
FR2305713A1 (fr) * 1975-03-25 1976-10-22 Faure Jean Distributeurs de volumes mesures de liquides
DE7518809U (de) * 1975-06-12 1978-09-14 Eppendorf Geraetebau Netheler & Hinz Gmbh, 2000 Hamburg Pipettenspitze

Also Published As

Publication number Publication date
JPH0457377B2 (enrdf_load_stackoverflow) 1992-09-11
AU3418284A (en) 1985-04-18
FI843976A0 (fi) 1984-10-10
JPS60137443A (ja) 1985-07-22
AU565474B2 (en) 1987-09-17
NO844093L (no) 1985-04-15
FI843976A7 (fi) 1985-04-14
DK490984D0 (da) 1984-10-12
DK490984A (da) 1985-04-14
FI843976L (fi) 1985-04-14
ATE45304T1 (de) 1989-08-15
EP0148333A1 (en) 1985-07-17
DE148333T1 (de) 1986-01-16
DE3479298D1 (en) 1989-09-14
NZ209865A (en) 1988-02-12

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