EP0080134B1 - Handbetätigter Wascher für Mehrlochplatten - Google Patents

Handbetätigter Wascher für Mehrlochplatten Download PDF

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Publication number
EP0080134B1
EP0080134B1 EP82110470A EP82110470A EP0080134B1 EP 0080134 B1 EP0080134 B1 EP 0080134B1 EP 82110470 A EP82110470 A EP 82110470A EP 82110470 A EP82110470 A EP 82110470A EP 0080134 B1 EP0080134 B1 EP 0080134B1
Authority
EP
European Patent Office
Prior art keywords
liquid supply
channel
pipes
wells
suction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
EP82110470A
Other languages
English (en)
French (fr)
Other versions
EP0080134A1 (de
Inventor
Jorgen Cappelen
Borge Nielsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nunc AS
Original Assignee
Nunc AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8138846&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP0080134(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Nunc AS filed Critical Nunc AS
Priority to AT82110470T priority Critical patent/ATE20196T1/de
Publication of EP0080134A1 publication Critical patent/EP0080134A1/de
Application granted granted Critical
Publication of EP0080134B1 publication Critical patent/EP0080134B1/de
Expired legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/02—Cleaning by the force of jets or sprays
    • A—HUMAN NECESSITIES
    • A47—FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
    • A47L—DOMESTIC WASHING OR CLEANING; SUCTION CLEANERS IN GENERAL
    • A47L17/00—Apparatus or implements used in manual washing or cleaning of crockery, table-ware, cooking-ware or the like
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L13/00—Cleaning or rinsing apparatus
    • B01L13/02—Cleaning or rinsing apparatus for receptacle or instruments
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates

Definitions

  • This invention relates to a manually operable apparatus for simultaneously washing a row of wells of a multi well plate, said apparatus comprising means for simultaneously supplying wash liquid to said wells and means for simultaneously discharging liquid from these wells.
  • This need is fulfilled with the washer of the type mentioned above which is disclosed in FR-A-2 358 206.
  • This prior art washer comprises means for supplying liquid to the wells of a micro test plate, which means perform their function independently however of the function of liquid discharge means for discharging liquid from the above-mentioned wells.
  • the prior art apparatus is based on the use of separate devices for introducing wash liquid into the chambers to be washed and for discharging liquid therefrom.
  • this object is achieved in that it comprises a manifold comprising a liquid supply channel which is connected with a source of wash liquid via a valve and which extends substantially parallel with a suction channel, the liquid supply channel being connected with a row of parallel liquid supply pipes and the suction channel being connected with a corresponding number of suction pipes, each suction pipe being mounted essentially parallel with and adjacent to a liquid supply pipe and in such a manner that a pair of pipes can be introduced into a well simultaneously.
  • washing liquid may be supplied to the liquid supply pipes and from these pipes it flows into the wells to be washed.
  • the washer In the initial phase of a washing procedure the washer is preferably held at such a distance above the wells that the free ends of the suction pipes are located at the level of the upper edges of the wells so as to prevent excessive amounts of washing liquid from flowing from one well into an adjacent well.
  • the valve When the wells have been filled, the valve is closed so as to stop the supply of liquid and the apparatus of the invention is moved downwardly to a position in which the lower ends of the suction pipes are located a short distance above the bottoms of the wells. During this movement liquid is continuously sucked out of the wells and the wells ordinarily are empty when the above mentioned lower position has been reached. The apparatus is then raised to the starting position and the washing operation may be repeated e.g. 2-3 times.
  • the ends of the suction pipes which pipes preferably are made from stainless steel, are preferably located at a longer distance from the manifold than the ends of the liquid supply pipes so as to allow the ends of the suction pipes to be moved down to the bottoms of wells having round bottoms without risking contact between the ends of the liquid supply pipes and the rounded bottom parts of said wells.
  • the manifold preferably is a block, e.g. of a plastics material, in which a bore forms a liquid supply channel which is located parallel to another bore forming the suction channel.
  • the liquid supply pipes e.g. thin pipes of stainless steel, are preferably mounted in the lower part of distributor channels in the form of bores extending from the top surface through the liquid supply channel to the bottom surface of the manifold block, the upper ends of said distributor channels being closed by removable closure means.
  • the closure means are preferably screws, such as Umbraco @ screws, having an external thread corresponding to an internal thread in the upper ends of said distributor channels.
  • a distributor channel can readily be cleaned in case a liquid supply pipe is blocked during use.
  • the screw in the upper end of the distributor channel is merely removed and a wire or a similar thin member is introduced into the distributor channel and the blocked liquid supply pipe.
  • liquid supply pipes may vary within broad ranges but when the washer of the invention is to be used in connection with multi well plates of the above mentioned type, 8 or 12 pipes are preferably employed.
  • the washer of the invention preferably comprises at least one and preferably two spacers provided at the underside of the manifold block. These spacers may consist of a fixed cylindrical core and an interchangeable bushing mounted on said core.
  • An 0-ring is preferably provided on the cylindrical cylinder surface of the core in order to maintain the bushing thereon in a fixed position.
  • the valve controlling the liquid supply to the liquid supply channel is preferably a spring activated valve which in its position of rest prevents washing liquid from flowing into the liquid supply channel and which can be opened by a slight finger pressure.
  • the valve is preferably mounted in a further bore in the manifold block and is maintained in said bore by a bottom screw which is screwed into said bore.
  • the means for connecting the liquid supply channel with the source of washing liquid and the means for connecting the suction channel with a source of vacuum are preferably pipe stubs made from a plastics material and inserted into bores in the manifold block.
  • a further important advantage of the apparatus of the invention is that in case the liquid supply channel or a liquid supply pipe is blocked, it can be cleaned simply by connecting the pipe stub communicating with the liquid supply channel with the source of vacuum.
  • a suitable source of vacuum is a water jet pump.
  • the washer illustrated in the drawings comprises a manifold in the form of a block 1 comprising two internal longitudinally extending channels, viz. a liquid supply channel 2 and a suction channel 3.
  • the liquid supply channel 2 is at one end connected with a bore 4 having provided therein a valve which will be described below.
  • the opposite end of the liquid supply channel 2 is closed by a screw 5 having an external thread corresponding to an internal thread in said channel.
  • the block 1 comprises 8 channels 6 extending from the top surface of the block 1 through the channel 2 and to the bottom surface of the block and perpendicular to the channel 2.
  • each channel 6 located above the channel 2 comprises an internal thread, and a screw 8 having an external thread is screwed into said portion 7.
  • a liquid supply pipe 9 extending some distance out from the block 1 is inserted in the lower portion of each channel 6.
  • a channel 13 connects the bore 4 with the exterior side of the block 1 and a pipe stub 14 is inserted in said channel 13.
  • Another channel 15 connects one end of the suction channel 3 with the exterior side of the block 1, and a pipe stub 16 is inserted in the channel 15.
  • the opposite end of the suction channel is closed by a screw 17 having an external thread corresponding to the internal thread of said channel.
  • the block 1 further comprises 8 channels extending perpendicularly from the suction channel 3 and each having inserted therein a suction pipe 18 extending a few millimetres longer out from the block 1 than the corresponding liquid supply pipes 9.
  • valve body 19 having a lower portion 20 of a greater diameter than the upper portion 21.
  • zone 22 In the zone between the upper portion 21 and the lower portion 20 of the valve body it comprises a zone 22 of reduced diameter.
  • An annular groove in which an O-ring 23 is mounted is provided on the exterior surface of the upper portion 21 of the valve body.
  • the underside of the valve body is hollow and the upper end of a coil spring 24 is inserted in said hollow portion.
  • the lower end of the coil spring 24 rests on a bottom screw 25 which is screwed into the lower portion of the bore 4 and which is sealed to the block 1 by means of an O-ring 26.
  • the lower portion of the bore 4 has a greater diameter than its upper portion and the lower portion of the valve body 19 cannot move upwardly through the bore 4.
  • the block 1 is sealed relative to the lower portion 19 of the valve body by means of an O-ring 27 which is provided in the transition zone between the upper and the lower portion of the bore 4.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Baking, Grill, Roasting (AREA)
  • Washing And Drying Of Tableware (AREA)
  • Electric Stoves And Ranges (AREA)
  • Electrical Discharge Machining, Electrochemical Machining, And Combined Machining (AREA)
  • Bolts, Nuts, And Washers (AREA)
  • Laminated Bodies (AREA)
  • Cleaning By Liquid Or Steam (AREA)
  • Water Treatment By Sorption (AREA)
  • Telephone Function (AREA)
  • Paper (AREA)

Claims (5)

1. Handbetätigbare Vorrichtung zum gleichzeitigen Waschen einer Lochreihe einer Mehrlochplatte, bestehend aus einer Einrichtung zum gleichzeitigen Zuführen einer Waschflüssigkeit zu den Löchern und einer Einrichtung zum gleichzeitigen Ableiten der Flüssigkeit von diesen Löchern, gekennzeichnet durch einen Verteiler (1) mit einem Flüssigkeitszuführkanal (2), der über ein Ventil (19) mit einer Waschflüssigkeitsquelle verbunden ist und sich im wesentlichen zu einem Saugkanal (3) erstreckt, wobei der Flüssigkeitszuführkanal mit einer Reihe paralleler Flüssigkeitszuführrohre (9) verbunden ist und wobei der Saugkanal mit einer entsprechenden Anzahl von Saugrohren (18) verbunden ist, von denen jedes im wesentlichen parallel und neben einem Flüssigkeitszuführrohr derart angeordnet ist, daß ein Paar Rohre (9, 18) gleichzeitig in ein Loch einführbar ist.
2. Vorrichtung nach Anspruch 1, dadurch gekennzeichnet, daß die Enden der Saugrohre (18) in einem größeren Abstand vom Verteiler (1) angeordnet sind als die Enden der Flüssigkeitszuführrohre (9).
3. Vorrichtung nach Anspruch 1, dadurch gekennzeichnet, daß der Verteiler aus einem Block (1) besteht, in dem eine Bohrung einen Flüssigkeitszuführkanal (2) bildet, der zu einer anderen, den Saugkanal (3) bildenden Bohrung parallel angeordnet ist, und in dem die Flüssigkeitszuführrohre (9) in die unteren Enden von Verteilungskanälen (6) eingesetzt sind, die sich von der Oberseite des Blockes (1) durch den Flüssigkeitszuführkanal (2) hindurch zur Unterseite des Blockes erstrecken, wobei der obere Bereich (7) der Verteilerkanäle (6) durch lösbare Verschlußteile (8) verschlossen ist.
4. Vorrichtung nach Anspruch 3, dadurch gekennzeichnet, daß zylindrische Distanzkerne (10) mit austauschbaren Umfangsbuchsen (12) an der Unterseite des Blockes (1) angeordnet sind.
5. Vorrichtung nach Anspruch 1, dadurch gekennzeichnet, daß das Ventil (19) in einer Bohrung (4) des Blockes (1) angeordnet ist, wobei die Bohrung mit dem Flüssigkeitszuführkanal (2) verbunden ist.
EP82110470A 1981-11-13 1982-11-12 Handbetätigter Wascher für Mehrlochplatten Expired EP0080134B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT82110470T ATE20196T1 (de) 1981-11-13 1982-11-12 Handbetaetigter wascher fuer mehrlochplatten.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK503381A DK147254B (da) 1981-11-13 1981-11-13 Manuelt betjeneligt skylleapparat til skylning af broende i en mikrotestplade
DK5033/81 1981-11-13

Publications (2)

Publication Number Publication Date
EP0080134A1 EP0080134A1 (de) 1983-06-01
EP0080134B1 true EP0080134B1 (de) 1986-06-04

Family

ID=8138846

Family Applications (1)

Application Number Title Priority Date Filing Date
EP82110470A Expired EP0080134B1 (de) 1981-11-13 1982-11-12 Handbetätigter Wascher für Mehrlochplatten

Country Status (7)

Country Link
EP (1) EP0080134B1 (de)
JP (1) JPS5892486A (de)
AT (1) ATE20196T1 (de)
CA (1) CA1181319A (de)
DE (1) DE3271585D1 (de)
DK (1) DK147254B (de)
NO (1) NO151919B (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8522872D0 (en) * 1985-09-16 1985-10-23 Flow Lab Multi-cavity washing apparatus
JPS63128259A (ja) * 1986-11-18 1988-05-31 Yasunobu Tsukioka 血液等の検査における反応ビ−ズの洗滌方法と洗滌装置
US5620894A (en) * 1995-06-16 1997-04-15 Glaxo Wellcome Inc. Apparatus for automated biological cell harvesting
CN1153004C (zh) 1999-06-18 2004-06-09 株式会社三协精机制作所 旋转式缸装置
JP6216298B2 (ja) * 2014-08-29 2017-10-18 株式会社日立ハイテクノロジーズ 自動分析装置及びそれを用いた分析方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1582434A (en) * 1976-05-13 1981-01-07 Secr Social Service Brit Analytical apparatus and methods incorporating cuvette cleaning and cleanliness testing
BE856748A (fr) * 1976-07-16 1977-10-31 Dynatech Worldmed Procede et dispositif pour le nettoyage de plaques de micro-test
DE3028746C2 (de) * 1980-07-29 1985-11-28 Guido 7919 Bellenberg Oberdorfer Hochdruck-Reinigungsanlage
DE3029440C2 (de) * 1980-08-02 1983-01-13 Ernst 4030 Ratingen Fooke Vorrichtung zum Waschen von Festkörpern

Also Published As

Publication number Publication date
NO822317L (no) 1983-05-16
DE3271585D1 (en) 1986-07-10
JPS5892486A (ja) 1983-06-01
DK147254B (da) 1984-05-28
CA1181319A (en) 1985-01-22
DK503381A (da) 1983-05-14
ATE20196T1 (de) 1986-06-15
NO151919B (no) 1985-03-25
EP0080134A1 (de) 1983-06-01

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